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Comprehensive Evaluation on Agricultural Environment in Western China

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Journal of Mountain Science 2005年第3期2卷 244-254页

作者： TU Jianjun deng yulin School of Resources and Environmental Science Southwest University Beibei 400715 Chongqing China Sichuan Agricultural University Ya＇an 625014 Sichuan China

Taking provinces as study cases, 15 comprehensive factors, involving natural geography, agricultural production, economic and social information, were formulated to evaluate the agricultural environment in western China by factor analysis method. The results showed that comprehensive scores were distinct among different cases. The score of Sichuan was the highest, that of Chongqing second and that of Guangxi the third. Qinghai got the lowest score, ranking the twelfth. The general environment quality was better in moist southern regions than that in frigid northern regions in the provinces of western China. Therefore, the appropriate eco-environmental construction measures at provincial level should be proposed to increase vegetation coverage, control water and soil loss, and prevent desertification and wind erosion. To develop eco-agriculture is an inevitable selection for environment construction and improvement in western China.

关键词：中国西部地区农业环境农业生态生态系统

Effects of cadmium,17 β-estradiol and their interaction in the male Chinese loach (Misgurnus anguillicaudatus)

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Chinese Science Bulletin 2012年第8期57卷 858-863页

作者： LÜ XueFei LIU FeiYuan ZHOU XiaoPing ZHOU QunFang deng yulin School of Life Science Beijing Institute of TechnologyBeijing 100081China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy ofSciencesBeijing 100085China Beijing Centre for Physical and Chemical Analysis Beijing 100094China

To assess the interaction effect between cadmium (Cd) and 17-estradiol (E2),male Chinese loaches (Misgurnus anguillicaudatus) were exposed to E2 (1 g/L) and Cd (50 or 500 g/L) alone and in combination using a semi-static waterborne exposure system.The effects of E2 on the accumulation and distribution of Cd,as well as the effects of Cd on vitellogenin (Vtg) synthesis induced by E2,were investigated.Cd mainly accumulated in the kidneys,liver,intestines,and gills,with little amounts in muscles,bones,and gonads.Co-exposure with E2 did not change the main targets for Cd.E2 could induce Vtg synthesis in male Chinese loaches,and co-exposure with 50 or 500 g/L Cd could inhibit the Vtg induced by 1 g/L E2.Compared with the results reported in the literature,it can be concluded that factors such as fish species,Cd dosage,and manner of exposure might make contributions to the interaction between Cd and E2.Our results also suggested that male Chinese loaches are susceptible to Cd,and can be recommended as a potential sentinel species to study the ecotoxicology of heavy metals.

关键词：镉(Cd) 泥鳅中国雌二醇卵黄蛋白原生态毒理学互动效应曝光系统

Space Life Science of China in 2013

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空间科学学报 2014年第5期34卷 747-756页

作者： LEI Runhong ZHOU Guangming MA Hong ZHUANG Fengyuan deng yulin School of Life Science Beijing Institute of Technology Department of Space Radiobiology Key Laboratory of Heavy Ion Radiation Biology and MedicineInstitute of Modern Physics Chinese Academy of Sciences

In the past two years, space life science research in China is characterized by a wide area of basic researches for providing foundation for the future China Space Station. The effect of microgravity and radiation was further studied from physiology phenomena to the level of bio-molecule mechanisms. Chinese space life science is maturing in a new era of comprehensive development.Here, we review and summarize researches on space life sciences which were contributed by Chinese scientists.

关键词： Space life sciences Simulated microgravity Ionizing radiation

Analysis of biomarkers for the cross-linkage of formaldehyde with bovine serum albumin peptides

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Chinese Science Bulletin 2008年第21期53卷 3287-3293页

作者： AHMAD Waqar deng yulin LI Bo LI LiLi AHAMD Manzoor IQBAL Zafar PARVEEN Zahida School of Life Science and Technology Beijing Institute of Technology Beijing 100081 China HEJ Research Institute of Chemistry University of Karachi Karachi-75270 Pakistan Department of Pharmacy University of Peshawar Peshawar-25000 Pakistan

Formaldehyde,a well-known environmental toxic hazard,has been found to produce endogenously via semicarbazide-sensitive amine oxidase-catalyzed oxidative deamination of methylamine.In diabetes,the activity of SSAO has been found to increase with a subsequent increase in endogenous formalde-hyde production.It has been postulated that SSAO-induced production of formaldehyde may be in-volved in the alteration of protein structure,which may subsequently cause protein deposition associ-ated with chronic pathological disorders.Formaldehyde has also been found to react(cross-link) with amino group of the N-terminal amino acid residue and with the side-chains of arginine,cysteine,his-tidine and lysine residues.Therefore,formaldehyde may be responsible,at least in part,for protein cross-linkage,oxidative stress and cytotoxicity.The cross-linking of formaldehyde with bovine serum albumin was studied using LC-MS and Mascot database.The peptides sequence for control BSA(un-treated) digested with trypsin was matched in the online database search query by exporting the MS/MS data to online MASCOT database.In this way,a total of twenty-seven peptides were matched in the database search query.These twenty-seven peptides were then searched manually in all of the tryptic BSA samples treated with different concentrations of FA that were incubated in different time intervals.Six formaldehyde-treated BSA peptides(FKDLGEEHFK,HLVDEPQNLIK,KVPQVSTPTLVEVSR,RPCFSALTPDETYVPK,LVNELTEFAK,DAFLGSFLYEYSR) were found to be the possible markers for formaldehyde-protein/peptides adducts.

关键词：甲醛牛血清白蛋白时标生物标志物

Preparation and evaluation of an enzymatic microreactor based on HILIC matrix for digestion and identification of proteins

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Chinese Science Bulletin 2010年第10期55卷 910-914页

作者： WANG ShanShan LI Qin LU XueFei LIU Tong ZHANG WeiBo GENG LiNa deng yulin School of Life Science and Technology Beijing Institute of Technology Beijing 100081 China Analysis and Test Center Shandong Academy of Sciences Jinan 250014 China

In the present study,a new enzymatic microreactor is developed by immobilizing trypsin on the matrix-HILIC (hydrophilic interaction chromatography).The influences of different types of buffer solutions (borate,Tris·HCl,and phosphate),their concentrations and pH values on the immobilizing rate and the activity of immobilized trypsin were investigated.The optimal condition for off-line immobilization of trypsin on matix-HILIC was Tris·HCl buffer (20 mmol/L,pH 8.0),and then,under the optimal condition,an enzymatic microreactor was prepared by trypsin dynamically immobilized on HILIC matrix which was packed in a Peeksil column.It can be found that the microreactor possessed high hydrolytic activity according to the hydrolysis results of N-α-benzoyl-DL-arginine P-nitroanilide (BAPNA),a substrate of trypsin.Moreover,bovine serum albumin (BSA),cytochrome C and myoglobin as standard proteins were chosen to characterize the enzymolysis of the microreactor demonstrating the high enzymolysis efficiency by this enzymatic microreactor.Besides,the deactivated trypsin can be easily eluted from the matrix-HILIC to re-immobilize trysin,which is highly cost-effective in the experiment.All the results show that the new enzymatic microreactor associated with HPLC-MS techniques can realize online enzymolysis of proteins,and the time to analyze and identify the proteins is greatly shortened.

关键词：酶催化反应动态矩阵蛋白质评价鉴定胰蛋白酶活性酶固定化消化制备

西藏中拉萨地块门巴花岗闪长岩成因及深部动力学过程

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地球科学 2024年第2期49卷 577-593页

作者：王旭辉郎兴海梁海辉杜良艺邓煜霖何青董咪成都理工大学地球科学学院四川成都610059 华东冶金地质勘查物探队安徽芜湖241000

为了探讨拉萨地块晚白垩世岩浆作用的深部动力学机制,对中拉萨地块南缘门巴花岗闪长岩开展了岩相学、年代学、岩石地球化学及矿物化学研究.LA-ICP-MS锆石U-Pb定年表明门巴花岗闪长岩侵位于晚白垩世(83.2 Ma±0.9 Ma).岩石地球化学特征... 详细信息

为了探讨拉萨地块晚白垩世岩浆作用的深部动力学机制,对中拉萨地块南缘门巴花岗闪长岩开展了岩相学、年代学、岩石地球化学及矿物化学研究.LA-ICP-MS锆石U-Pb定年表明门巴花岗闪长岩侵位于晚白垩世(83.2 Ma±0.9 Ma).岩石地球化学特征显示门巴花岗闪长岩为埃达克质岩石,电子探针数据揭示斜长石属于中-奥长石(An:16.2~34.7).综合分析本文认为门巴花岗闪长岩的母岩浆为幔源镁铁质岩浆底侵诱发加厚下地壳熔融并与之发生混合作用的结果.结合晚白垩世岩浆岩成岩环境及时空分布特征,认为拉萨地块南部晚白垩世岩浆作用主要受新特提斯洋脊俯冲控制,软流圈地幔沿洋中脊裂隙板片窗上涌诱引了南拉萨地块南缘晚白垩世大规模岩浆作用,而软流圈物质沿切割洋中脊的转换断层撕裂板片窗上涌诱发了近似垂直前者分布的小规模板内岩浆作用.

关键词：拉萨地块晚白垩世埃达克质岩石岩浆混合洋脊俯冲板片窗岩石学

Breaking pore size limit of metal–organic frameworks:Bio-etched ZIF-8 for lactase immobilization and delivery in vivo

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Nano Research 2022年第6期15卷 5646-5652页

作者： Xiaoyue Qi Qizhe Chen Ziyong Chang yulin deng School of Medical Technology Beijing Institute of TechnologyBeijing 100081China Institute of Engineering Medicine Beijing Institute of TechnologyBeijing 100081China Institute of Chemistry Chinese Academy of SciencesBeijing 100190China Civil and Resource Engineering School University of Science and Technology BeijingBeijing 100083China School of Life Science Beijing Institute of TechnologyBeijing 100081China

Expanding pore size range of metal–organic frameworks(MOFs)promotes their versatility and feasibility for various biomedical applications.However,natural pore size greatly restricts large guest molecule accommodation.Customizing and tailoring pore apertures ranging from micropores to mesopores controllably is desired but still critically challenging.Herein,we developed a facile method with super mildness based on pH-sensitive zeolitic imidazolate framework(ZIF)-8 to increase porosity,providing pore size with maximum 20 nm,which is 8 times larger than average.Glucose oxidase(GOx)was introduced in ZIF-8 for bioetching,benefitted from the resultant acidic microe-nvironment during biocatalytic process.Different synthesis methods were assessed for obtaining different morphologies and size distributions.Reaction time,GOx encapsulation efficiency,and Zn2+concentration was optimized to precisely control the mesopore size distribution of MOFs.It was found that bio-etching strategy was capable of producing stable mesopores which were large enough for loading lactase with good enzymatic activity retained,verified both in vitro and in vivo.This strategy breaks natural pore size limitation of MOFs and thereby facilitates biomolecule delivery,catalysis,and other biomedical applications with enhanced stability and performance.

关键词： metal-organic frameworks enzyme immobilization drug delivery oral biocompatibility

An ultra-fast and highly efficient multiple proteases digestion strategy using graphene-oxide-based immobilized protease reagents

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Science China Chemistry 2014年第5期57卷 695-702页

作者： BAI HaiHong PAN YiTing REN XiaoJun HAO FeiRan deng ShanShan FAN Chao YAN Hui SHEN BingQuan MA Lin TIAN Fang PENG Bo deng yulin QIN WeiJie QIAN XiaoHong School of Life Science and Technology Beijing Institute of Technology National Center for Protein Sciences Beijing State Key Laboratory of Proteomics Beijing Proteome Research CenterBeijing Institute of Radiation Medicine

Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in shotgun-based proteome-analysis strategy.Tandem digestion by two or more proteases is demonstrated to be helpful for increasing digestion efficiency and decreasing missed cleavages,which results in more peptides that are compatible with mass-spectrometry analysis.Compared to conventional solution digestion,immobilized protease digestion has the obvious advantages of short digestion time,no self-proteolysis,and reusability.We proposed a multiple-immobilized proteases-digestion strategy that combines the advantages of the two digestion strategies mentioned above.Graphene-oxide(GO)-based immobilized trypsin and endoproteinase Glu-C were prepared by covalently attaching them onto the GO surface.The prepared GO-trypsin and GO-Glu-C were successfully applied in standard protein digestion and multiple immobilized proteases digestion of total proteins of Thermoanaerobacter tengcongensis.Compared to 12-hour solution digestion using trypsin or Glu-C,14%and 7%improvement were obtained,respectively,in the sequence coverage of BSA by one-minute digestion using GO-trypsin and GO-Glu-C.Multiple immobilized-proteases digestion of the total proteins of Thermoanaerobacter tengcongensis showed 24.3%and 48.7%enhancement in the numbers of identified proteins than was obtained using GO-trypsin or GO-Glu-C alone.The ultra-fast and highly efficient digestion can be contributed to the high loading capacity of protease on GO,which leads to fewer missed cleavages and more complete digestion.As a result,improved protein identification and sequence coverage can be expected.

关键词：固定化蛋白酶消化时间超快速氧化物固定化胰蛋白酶石墨蛋白质组试剂

基于生物信息学分析通用转录因子IIH对前列腺癌预后的影响

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中华医学杂志 2024年第16期104卷 1410-1417页

作者：李劲创朱学进叶剑恒谭泽恒蔡尚桦邓煜麟陈坚田威城罗丹骅钟惟德华南理工大学附属第二医院泌尿外科广州510180 广州医科大学附属肿瘤医院泌尿外科广州510095 澳门科技大学中医药学院中国澳门999078 南方医科大学珠江医院泌尿外科广州510280 广州医科大学附属第一医院广州510230

目的探讨转录起始前复合物中通用转录因子IIH(TFIIH)在前列腺癌(PCa)中的遗传、表达特征及其与PCa预后的关系。方法通过分析癌症基因图谱-PCa数据库(TCGA-PRAD)中495例PCa组织及52例癌旁组织TFIIH亚基的表达特征及临床意义。再通过PCa... 详细信息

目的探讨转录起始前复合物中通用转录因子IIH(TFIIH)在前列腺癌(PCa)中的遗传、表达特征及其与PCa预后的关系。方法通过分析癌症基因图谱-PCa数据库(TCGA-PRAD)中495例PCa组织及52例癌旁组织TFIIH亚基的表达特征及临床意义。再通过PCa微阵列芯片验证TFIIH中的关键因子通用转录因子IIH亚基4(GTF2H4)与临床特征[病理分期、生化复发(BCR)以及格里森评分]的相关性。结果495例PCa患者年龄为(61.01±6.82)岁。ERCC3、GTF2H4、MNAT1在格里森评分≥8的PCa组织中mRNA表达量较高(P0.7],并在额外4个数据库中得到验证。480例PCa样本中,9例发生了TFIIH亚基的突变,占比1.87%。其中3例为ERCC2亚基突变,2例为GTF2H3亚基突变,GTF2H1、GTF2H4、CCNH、CDK7亚基突变各1例。单因素Cox回归分析显示GTF2H4为BCR的危险因素(HR=2.470,95%CI:1.620~3.767,P<0.001),GTF2H5为保护因素(HR=0.506,95%CI:0.336~0.762,P=0.001)。免疫组织化学染色评分结果显示GTF2H4的蛋白表达量与患者临床特征相关,差异均有统计学意义。结论TFIIH中的关键因子GTF2H4在PCa中高表达并提示预后欠佳。

关键词：前列腺肿瘤通用转录因子IIH 生化复发