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Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate

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Science China Chemistry 2016年第2期59卷 237-242页

作者： Pengjuan Wang Ying Wan Aldalbahi Ali shengyuan deng Yan Su Chunhai Fan Shulin Yang School of Mechanical Engineering Nanjing University of Science and Technology Nanjing 210094 China School of Envtronmental and Biologtcal and Engmeermg Nanjing University of Science and Technology Nanjing 210094 China Chemistry Department King Saud Universlty Riyadh 1145 Saudt Arabia Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China

Organophosphorous pesticide(OP) contamination has serious adverse effects on human health and the environment. Due to the toxicity of OPs and the threat presented by their accidental or intentional release in populated areas, the determination and monitoring of these OPs in food products and environment is of great importance. OPs are present in very small quantities and therefore, methods for their detection need to be highly sensitive and selective. Here, we aimed to develop a simple and selective aptamer-based colorimetric assay for the detection of omethoate, which is one of the commonly used OPs. The principle of the assay is that single-stranded DNA(ss DNA)-wrapped gold nanoparticles(Au NPs) are resistant to salt-induced aggregation. By employing an "artificial antibody" organophosphorous pesticide-binding aptamer(OBA) as the recognition element, aptamer-wrapped Au NPs(Au-apta) show high selectivity towards omethoate, resulting in the disconnection of aptamers from Au NPs and the aggregation of Au NPs. As there is a significant color change from the interparticle plasmon coupling during the aggregation of Au NPs, the established assay showed good linearity between 0.1 and 10 μmol/L, with a low detection limit of 0.1 μmol/L. Other OPs such as profenofos, phorate, and isocarbophos would not interfere with the detection of omethoate despite having similar structures. Thus, the colorimetric method shows potential for use in the detection of omethoate in real soil samples.

关键词： aptamer DNA gold nanoparticles omethoate

Mobile mini-fluorimeter for antibiotic aptasensing based on surface-plasmonic effect of burlike nanogolds enhanced by digitized imaging diagnosis

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Chinese Chemical Letters 2024年第5期35卷 418-422页

作者： Tiantian Man Fulin Zhu Yaqi Huang Yuhao Piao Yan Su shengyuan deng Ying Wan School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing 210094China Key Laboratory of Metabolic Engineering and Biosynthesis Technology Ministry of Industry and Information TechnologySchool of Environmental and Biological EngineeringNanjing University of Science and TechnologyNanjing 210094China

Antibiotic abuse now poses a grave threat to global ecology and bestirs public concerns about the residue issue in daily *** traceability measurements along supply chain or logistic circulation have become increasingly essential given the labile nature of diverse synthetic residuals on *** an attempt to answer this urgency,here a miniaturized fluorometric aptasensor prototype was contrived that catered to the point-of-care screening norm for two typical additives:chloramphenicol and *** key target-indicating module worked in vitro based on the competitive binding-induced fluorescence recovery of fluorescein-labeled aptamers,which were photobleached beforehand in the format of double helix on burlike nanogold ***“prickly”geometry of the latter not just enriched the capture probes at preferentially substrate-accessible spires;but also contributed to a tip-enhanced surface plasmon effect,sensitizing the signal-on during the duplex dissociation even at nanomolar threshold of the *** the other hand,to encompass a full portable,a set of optical devices were mounted within a 3D-printed cartridge(adaptor)to converge the light beam and route it towards the detector,for which the smartphone camera came up in handy with a home-developed App for calibrating the emissive *** by the high-dynamic-range compression,an imaging diagnostic algorithm was built in to grid and digitize each slide in the album for augmented detection ***,a novel bio-to-silico integration was invented that capable of in situ rapid reporting on the antibiotic presence with high sensitivity and *** field practices in spiked milk on sales proved the precision and rudimentary feasibility of the well-assembled model of appliance,thus holding nice prospects in nonexpert(e.g.,family and local community)utilities for foodborne antibiotic identification.

关键词： Chloramphenicol and enrofloxacin Aptasensor Gold nanoburs Smartphone fluorimetric suite Digitized image postprocessing

Specific Electrochemiluminescence of Aptamer-Functionalized Quantum Dots with Lysozyme and Hemin as Co-Triggers

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Chinese Journal of Chemistry 2016年第3期34卷 331-336页

作者： Xubo Ji Chuanguang Yao Ying Wan Hongxin Song Peng Xin Hongda Cui Chenyu Zheng shengyuan deng School of Environmental and Biological and Engineering Nanjing University of Science and Technology Nanjing Jiangsu 210094 China School of Mechanical Engineering Nanjing University of Science and Technology Nanjing Jiangsu 210094 China State Key Laboratory of Analytical Chemistry for Life Science Department of Chemistry Nanjing University Nanjing Jiangsu 210093 China

This work proposed a label-flee, cost-effective and fairly sensitive electrogenerated chemiluminescence （ECL） strategy for the specific detection of lysozyme based on the hemin/G-quadruplex hybrid. Gold nanoparticles were spread onto the chitosan thin-film as substrate to adsorb thiolated captures： a hairpin-structured DNA, integrating dual head-tail connected functional domains： one aptamer sequence for lysozyme and the other for hemin （iron（II1） proto-porphyrin IX）. In the presence of the both, the hairpin conformation unfolded and transformed into the hemird G-quadruplex motif, which quenched the ECL emission of underlaid quantum dots significantly via the consump- tion of dissolved oxygen as endogenous coreactant. This construction enabled a wide linear response to lysozyme, ranging from 20 μ***-1 to 5μg. mL-1, with a detection limit as low as 4.95 pgo mL-1 （e.g. 9.4 pmol·L^-1）, dem- onstrating the prospective utilization of DNA technologies in bioanalysis.

关键词： aptasensor lysozyme G-quadruplex hemin electrochemiluminescence

A writable lithium metal ink

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Science China Chemistry 2020年第10期63卷 1483-1489页

作者： Wangyan Wu Jian Duan Jiayun Wen Yuwei Chen Xuyang Liu Liqiang Huang Zhengfeng Wang shengyuan deng Yunhui Huang Wei Luo Institute of New Energy for Vehicles School of Materials Science and EngineeringTongji UniversityShanghai 201804China

The recent revival of Li metal anodes(LMA)leads to a renewed interest in LMA as the ultimate choice for rechargeable lithium batteries towards high energy ***,multiple challenges stand in the way of using LMA,of which high reactivity,dendrite growth,the difficulty of fabricating Li thin foils,and the flammability of organic liquid electrolytes are ***,a writable Li metal ink(LMI)prepared by introducing biomass-derived carbon particles into molten Li is *** to the significantly decreased surface tension,LMI is able to directly write on copper foils or other substrates that ultrathin Li foils with a remarkably small thickness(<10μm)can be *** versatility of LMI is further demonstrated in addressing the interface issue between LMA and garnet-type solid-state electrolytes,where directly writing LMI on the garnet offers a perfect contact and enables an extremely low interfacial resistance of 6Ωcm^2,in sharp contrast to 939Ωcm^2 between the pure Li and the *** to the successful partnership with non-flammable solid-state electrolytes,ink-based technology may have a chance to bring us very close to the use of solid-state lithium metal batteries(SSLMBs)with high safety and high energy density.

关键词： lithium metal ink surface tension solid-state batteries interface controllable thickness

Handheld Aptasensor for Sandwiched Detection of Chloramphenicol

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Chemical Research in Chinese Universities 2020年第2期36卷 291-295页

作者： YU Mintong XIA Tian BAI Wanchen JI jinyu WANG Huan HUANG Yaqi deng shengyuan MA Kefeng SU Yan WAN Ying School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing 210094P.R.China Key Laboratory of New Membrane Materials Ministry of Industry and Information TechnologySchool of Environmental and Biological EngineeringNanjing University of Science and Technology/Nanjing 210094R R.China

Chloramphenicol(CAP)is an antifungal agent approved for use in ***,CAP residue imparts serious adverse effect on human health as well as the ***,keeping track of CAP in aquatic products and its environmental metabolites are of great ***,we developed a novel and neat electrochemical aptasensor for the detection of simulated CAP residual in a sandwich *** screen-printed carbon electrode was employed as the sensing platform and electrodeposited with peculiar nanogolds to enhance the electrochemical *** the specific recognition of such small target,a pair of chloramphenicol-binding aptamers,the primary Aptl and the secondary Apt2,were tailored as an artificial single-chained antibody couple,of which the thi-olated Aptl stood on the coated working substrate to capture the analyte,while the biotinylated Apt2 rallied the signal *** the presence of CAP,Aptl at the lower place cooperated with the upper Apt2 pinched CAP *** that,the reporter avidin-conjugated horseradish peroxidase associated atop the mounted biotin labels,resulting in an electrocatalytic *** whole,this modular biosensing construction would set a paradigm not just for practicable checkpoint quarantine or point-of-care testing,but potentially adaptable for technological fusion with“Internet Plus”and“Internet of Things”.

关键词： Chloramphenicol Screen-printed carbon electrode Electrochemical aptasensor Electrochemical deposi tion Ufkimm

Point-of-Care Testing of Chloramphenicol in Food Production Using Smartphone-Based Electrochemical Detector

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Journal of Analysis and Testing 2023年第1期7卷 33-39页

作者： Changqi Geng Yaqi Huang Bin Li Yueyu Wang Longyi Zhu Yuyan Xu Kejiao Gao Yao Mu Yan Su shengyuan deng Ying Wan School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing 210094China School of Environmental and Biological Engineering Nanjing University of Science and TechnologyNanjing 210094China School of Chemistry and Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094China

Antibiotic abuse in food processing could threaten human health via either direct pathogen or drug-resistant *** curb this situation,it is of vital significance to identify trace level of antibiotics in foodstuffin a point-of-care inspection ***,a smartphone-based electrochemical system was developed for the quantitation of chloramphenicol(CAP).The differential pulse voltammetric reduction of CAP was measured facilely on a piece of gold-deposited screen-printed electrode,which was docked onto a palm-sized detector with remote directives from a mobile APP of Bluetooth handshaking and digital *** optimal conditions,the target CAP could be determined in a range from 1 nmol/L to 5μmol/L with a detection limit of 0.25 nmol/*** tests on emulate samples demonstrated the miniaturized device could handle the food-screening scenarios with accuracy,convenience and quick response.

关键词： Chloramphenicol Electrodeposition Electrochemical detector Screen-printed electrode

Coherent V^(4+)-rich V_(2)O_(5)/carbon aerogel nanocomposites for high performance supercapacitors

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Science China Materials 2022年第7期65卷 1797-1804页

作者： Wenchao Bi shengyuan deng Haisha Tang Yuan Liu Jun Shen Guohua Gao Guangming Wu M.Atif MS AlSalhi Guozhong Cao Departments of Physics College of ScienceUniversity of Shanghai for Science and TechnologyShanghai 200093China Shanghai Key Laboratory of Special Artificial Microstructure School of Physics Science and EngineeringTongji UniversityShanghai 200092China Department of Materials Science and Engineering University of WashingtonSeattleWA 98195-2120United States Research Chair on Laser Diagnosis of Cancers Department of Physics and AstronomyCollege of ScienceKing Saud University11451RiyadhSaudi Arabia Department of Physics and Astronomy College of ScienceKing Saud University11451RiyadhSaudi Arabia

V^(4+)-rich V_(2)O_(5)/carbon aerogel(V^(4+)-V_(2)O_(5)/CA)composites were synthesized as electrode materials for supercapacitors via in-situ hydrolysis-condensation *** aerogel(CA)promotes the deposition of amorphous vanadium oxide and catalyzes the crystallization of V^(4+)-rich V_(2)O_(5)nanosheets at much reduced *** a uniform distribution of V_(2)O_(5)nanosheets in CA,the special structure provides a large specific area and reactive *** also synergistically improves the electrical conductivity and structural *** addition,rich V^(4+) would enhance the intrinsic electrical conductivity of V_(2)O_(5),promote ion diffusion and catalyze the electrochemical ***,V^(4+)-V_(2)O_(5)/CA exhibits much enhanced specific capacitance(405 F g^(-1) at 0.5 A g^(-1)),high energy density(56 W h kg^(-1) with a power density of 250 W kg^(-1))and long cycle life(96%capacitance retention after 40,000 cycles).

关键词： V_(2)O_(5) V^(4+) carbon aerogel kinetics supercapacitor

电沉积石墨烯的非共价功能化用于SPR核酸传感的协同增敏

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化工学报 2016年第S2期67卷 245-254页

作者：袁培新郑晨昱崔宏达万莹姚传广宋宏鑫邓盛元南京理工大学环境与生物工程学院江苏南京210094 南京理工大学机械工程学院江苏南京210094

研究了一种高效的表面等离子共振(SPR)基DNA分析方法,利用非共价功能化的石墨烯纳米片作为基底,并以酶催化诱导的聚合作为质量中继。该策略有多方面的目标:首先,通过原位优化的石墨烯薄膜的电生成,该过程由原子力显微拓扑图表征,来敏化... 详细信息

研究了一种高效的表面等离子共振(SPR)基DNA分析方法,利用非共价功能化的石墨烯纳米片作为基底,并以酶催化诱导的聚合作为质量中继。该策略有多方面的目标:首先,通过原位优化的石墨烯薄膜的电生成,该过程由原子力显微拓扑图表征,来敏化总体的SPR输出;其次,用于调制镍离子螯合的胺基三乙酸支架,其吸附在石墨烯支撑的苝基衍生物表面,上端生物素化捕获探针可以自组装,并保有一定的方向;最后,通过辣根过氧化物酶标记的报告单元,实时地将苯胺添加剂转化为聚苯胺沉淀,以协同实现信号的放大。运用上述配置,获得了一个对特异性DNA靶标精确且可重现传感的平台,检测下限达到飞摩尔水平,从而展现了以二维纳米材料为独特SPR基础设施的有益探索和开发。该"自下而上"的建构、与置顶"自上而下"的重量反应器,极有可能拓展并移植用于蛋白质的定量。

关键词：表面等离子体共振脱氧核糖核酸电化学还原石墨烯纳米片微反应器苝接枝胺基三乙酸聚合

Calibration of Discrete Element Heat Transfer Parameters by Central Composite Design

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Chinese Journal of Mechanical Engineering 2017年第2期30卷 419-427页

作者： Zongquan deng Jinsheng CUI Xuyan HOU shengyuan JIANG Department of Mechatronics Engineering Harbin Institute ofTechnologyHarbin 150006China

The efficiency and precision of parameter calibration in discrete element method （DEM） are not satisfactory, and parameter calibration for granular heat transfer is rarely involved. Accordingly, parameter calibration for granular heat transfer with the DEM is studied. The heat transfer in granular assemblies is simulated with DEM, and the effective thermal conductivity （ETC） of these granular assemblies is measured with the transient method in simulations. The measurement testbed is designed to test the ETC of the granular assemblies under normal pressure and a vacuum based on the steady method. Central composite design （CCD） is used to simulate the impact of the DEM parameters on the ETC of granular assemblies, and the heat transfer parameters are calibrated and compared with experimental data. The results show that, within the scope of the considered parameters, the ETC of the granular assemblies increases with an increasing particle thermal conductivity and decreases with an increasing particle shear modulus and particle diameter. The particle thermal conductivity has the greatest impact on the ETC of granular assemblies followed by the particle shear modulus and then the particle diameter. The calibration results show good agreement with the experimental results. The error is less than 4%, which is within a reasonable range for the scope of the CCD parameters. The proposed research provides high efficiency and high accuracy parameter calibration for granular heat transfer in DEM.

关键词： Granular assembly · Parameter calibration · Effective thermal conductivity (ETC) · Discrete element method (DEM) · Central composite design (CCD) · Vacuum