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A fully automated centrifugal microfluidic system for sample-to-answer viral nucleic acid testing

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Science China Chemistry 2020年第10期63卷 1498-1506页

作者： Fei Tian Chao Liu Jinqi deng Ziwei Han lu Zhang Qinghua Chen Jiashu Sun CAS Key Laboratory of Standardization and Measurement of Nanotechnology CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Department of Chemistry Capital Normal UniversityBeijing 100048China

The outbreak of virus-induced infectious diseases poses a global public-health *** acid amplification testing(NAAT)enables early detection of pandemic viruses and plays a vital role in preventing onward ***,the requirement of skilled operators,expensive instrumentation,and biosafety laboratories has hindered the use of NAAT for screening and diagnosis of suspected *** we report development of a fully automated centrifugal microfluidic system with sample-in-answer-out capability for sensitive,specific,and rapid viral nucleic acid *** release of nucleic acids and the subsequent reverse transcription loop-mediated isothermal amplification(RT-LAMP)were integrated into the reaction units of a microfluidic *** whole processing steps such as injection of reagents,fluid actuation by rotation,heating and temperature control,and detection of fluorescence signals were carried out automatically by a customized *** validate the centrifugal microfluidic system using oropharyngeal swab samples spiked with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)armored RNA *** estimated limit of detection for armored RNA particles is 2 copies per reaction,the throughput is 21 reactions per disc,and the assay sample-to-answer time is approximately 70 *** enclosed and automated microfluidic system efficiently avoids viral contamination of aerosol,and can be readily adapted for virus detection outside the diagnostic laboratory.

关键词： nucleic acid testing virus microfluidics sample-to-answer automation

2014~2019年广州地区血液肿瘤儿童血培养病原菌分布及耐药性特点分析

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现代检验医学杂志 2020年第5期35卷 82-86页

作者：王洁琳周珍文高飞高秀蓉邓露露梁秉绍朱素菲广州市妇女儿童医疗中心检验科广州510623

目的探讨本中心儿童血液肿瘤患者血培养病原菌的分布及耐药特点,为临床合理诊疗提供参考。方法对该中心2014年~2019年儿童血液肿瘤科室送检血培养样本所分离的病原菌以及药敏结果进行回顾性分析。结果9396例患儿血培养标本共分离出病原... 详细信息

目的探讨本中心儿童血液肿瘤患者血培养病原菌的分布及耐药特点,为临床合理诊疗提供参考。方法对该中心2014年~2019年儿童血液肿瘤科室送检血培养样本所分离的病原菌以及药敏结果进行回顾性分析。结果9396例患儿血培养标本共分离出病原菌538株,其中革兰阴性菌253株(47.03%)、革兰阳性菌242株(44.98%)、真菌43株(7.99%)。革兰阴性菌中以肺炎克雷伯菌(11.90%)、大肠埃希菌(10.04%)和铜绿假单胞菌(5.39%)为主,三者对氨苄西林及一代头孢均表现出极高的耐药率,而厄他培南、美罗培南等碳青霉烯类抗生素对三种革兰阴性菌保持了较好的抗菌活性。2014~2019年肺炎克雷伯菌对氨苄西林/舒巴坦、头孢他啶、头孢吡肟、亚胺培南的耐药率差异有统计学意义(χ^2=11.328~15.203,P<0.05)。革兰氏阳性菌以凝固酶阴性葡萄球菌(17.47%)、缓症链球菌(10.60%)和金黄色葡萄球菌(4.28%)为主;耐甲氧西林金黄色葡萄球菌分离率为30.80%,对青霉素G的耐药率高达100%,但对万古霉素、利奈唑胺及喹奴普汀均表现为敏感。真菌中热带假丝酵母菌(6.69%)占主要比例。结论儿童血液肿瘤患者感染的病原菌种类较多,多重耐药感染情况比较常见。明确病原菌种类及药敏结果,合理应用抗生素,对有效控制血液感染有重要意义。

关键词：儿童血液肿瘤败血症耐药性

Enhanced performance triboelectric nanogenerator based on porous structure C/MnO_(2)nanocomposite for energy harvesting

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Nano Research 2022年第8期15卷 7163-7171页

作者： Honghao Zhang Ping Zhang Pengfei Li lu deng Weikang Zhang Baocheng Liu Zhengchun Yang School of Electrical and Information Engineering Tianjin UniversityTianjin 300072China School of Integrated Circuit Science and Engineering Adv.Mater.and Printed Electronics CenterTianjin Key Laboratory of Film Electronic&Communication DevicesTianjin University of TechnologyTianjin 300384China

Triboelectric nanogenerator(TENG)can realize a variety of mechanical energy collections in the environment,which has great potential in the field of wearable ***,many TENGs could rarely be satisfactory to wearable electronics promotion because of their expensive raw materials and complex manufacturing *** this study,a type of porous structure carbon powder/manganese dioxide(C/MnO_(2))nanocomposite is *** material adopts low-cost,high-yield carbon powder,can be prepared in one step through a simple,economical,and environmentally friendly hydrothermal preparation process,and has high economic *** power generation performance was obtained by modulating the charge trapping ability and storage capacity of polydimethylsiloxane@C/MnO_(2)(PDMS@C/MnO_(2))film based on variations in the weight-loading of C/MnO_(2).The maximum output voltage of carbon powder/manganese dioxide TENG(CM-TENG)is 63 V,which is 2.1 times that of PDMS-TENG and 1.86 times that of carbon powder TENG(C-TENG)and can easily light up 53 ***,CM-TENG can convert biological motion energy into electrical signals to detect human hand *** CM-TENG self-powered system can successfully drive various microelectronic devices,such as electronic watches,liquid crystal displays(LCDs),and *** study provides a reliable,low-cost,high-performance,and widely applicable electronic system that shows great potential in future fields such as wearable devices and micro-sensing systems.

关键词： porous structure carbon powder/manganese dioxide(C/MnO_(2)) nanocomposite triboelectric nanogenerator widely applicable microelectronic devices

Advances in Delivering Oxidative Modulators for Disease Therapy

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Research 2023年第2期2022卷 115-138页

作者： Wei Yang Hua Yue Guihong lu Wenjing Wang Yuan deng Guanghui Ma Wei Wei StateKey Laboratoryof BiochemicalEnginring Institute of Proess EngineeringChinese Academyof SciencesBejingChina School of Chemical Engineering University of Chinese Academy of SciencesBeijingChina Department of Orthopedics Fourth Medical CenterGeneral Hospital of Chinese PLABeijingChina

Oxidation modulators regarding antioxidants and reactive oxygen species(ROS)inducers have been used for the treatment of many ***,a systematic review that refers to delivery system for divergent modulation of oxidativ... 详细信息

关键词： oxidative system Oxidation

Design and Fabrication of Reflective Phase Shifter for Two-Dimensional Terahertz Beam-Scanning Reflectarray

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Chinese Journal of Electronics 2022年第3期31卷 581-588页

作者： YANG Jun YIN Mingjun GAO Haoyu YIN Zhiping YE Yang deng Guangsheng lu Hongbo Special Display and Imaging Technology Innovation Center of Anhui Province Academy of Opto-electric TechnologyHefei University of Technology Guangxi Key Laboratory of Wireless Wideband Communication and Signal Processing Guilin University of Electronic Technology Space Star Technology Co. Ltd.

A reflective phase shifter is proposed to realize the two-dimensional beam-scanning *** reflectarray is composed of double-dipole resonant elements in which the phase shift is implemented by applying a driving voltage to the liquid crystal(LC). A 30 ×30 phase shifter sample is fabricated on a quartz substrate with a 480 μm thickness and a 4 cm × 4 cm *** to the experimental results, 0 to 360° phase shift can be achieved within the bandwidth of 5 GHz. In order to accomplish two-dimensional beam-scanning and reduce the negative impact of driving lines on the reflectarray, a new LC driving method is developed. To verify the accuracy of the proposed approach, three samples of different driving line numbers are designed using the above-mentioned phase shifter, which all have achieved a0-360° phase shift in the bandwidth of 4 GHz. Considering the influence of LC anisotropy and inhomogeneity, an improved calculation result is obtained and compared with experimental data.

关键词： liquid crystals phase shifters above-mentioned phase shifter reflective phase shifter double-dipole resonant elements submillimetre wave antennas LC anisotropy two-dimensional terahertz beam-scanning reflectarray driving voltage dipole antenna arrays scanning antennas driving line numbers LC driving method reflectarray antennas

Antimony transformation and mobilization from stibnite by an antimonite oxidizing bacterium Bosea sp. AS-1

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Journal of Environmental Sciences 2022年第1期34卷 273-281页

作者： Li Xiang Chaoyang Liu deng Liu Liyuan Ma Xuan Qiu Hongmei Wang Xiaolu lu School of Environmental Studies China University of Geosciences(Wuhan)Wuhan 430074China State Key Laboratory of Biogeology and Environmental Geology China University of Geosciences(Wuhan)Wuhan 430074China

Soils and waters are heavily contaminated by antimony in Xikuangshan(XKS)mine *** is widely accepted that oxidative dissolution of sulfide minerals and aqueous dissolution are the most prevalent geochemical mechanisms for the release of Sb to the *** ***-1 is an antimonite-oxidizer isolated from the mine slag in Xikuangshan Sb *** genome sequencing revealed the presence of multiple sulfur-oxidizing genes,antimony(Sb)metabolism genes and carbon fixation genes in AS-1 s *** therefore hypothesized that under oxic conditions,AS-1 could mediate the oxidation of sulfide and Sb(Ⅲ)in stibnite(Sb_(2)S_(3))and lead to the release of ***,strain AS-1 was discovered as an autotrophic Sb(Ⅲ)-*** mobilization studies conducted with strain AS-1showed significantly enhanced mobilization of Sb,and complete oxidation of released Sb and sulfur to Sb(V)and *** addition,AS-1 induced a faster release of Sb under heterotrophic condition,and new acicular minerals might *** findings support the hypothesis that microorganisms play an important role in the mobilization and transformation of Sb in XKS mine area and may contribute to our further understanding of the Sb biogeochemical redox cycle in natural environment.

关键词： Antimonite oxidation Bosea sp.AS-1 Stibnite Antimony mobilization Xikuangshan mine area

3D core-shell nanofibers framework and functional ceramic nanoparticles synergistically reinforced composite polymer electrolytes for high-performance all-solid-state lithium metal battery

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Chinese Chemical Letters 2024年第8期35卷 425-432页

作者： Hengying Xiang Nanping deng lu Gao Wen Yu Bowen Cheng Weimin Kang State Key Laboratory of Separation Membranes and Membrane Processes Tiangong UniversityTianjin 300387China School of Textile Science and Engineering Tiangong UniversityTianjin 300387China

Satisfactory ionic conductivity,excellent mechanical stability,and high-temperature resistance are the prerequisites for the safe application of solid polymer electrolytes(SPEs)in all-solid-state lithium metal batteries(ASSLMBs).In this study,a novel poly(m-phenylene isophthalamide)(PMIA)-core/poly(ethylene oxide)(PEO)-shell nanofiber membrane and the functional Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)ceramic nanopar-ticle are simultaneously introduced into the PEO-based SPEs to prepare composite polymer electrolytes(CPEs).The core PMIA layer of composite nanofibers can greatly improve the mechanical strength and thermal stability of the CPEs,while the shell PEO layer can provide the 3D continuous transport channels for lithium *** addition,the introduction of functional LLZTO nanoparticle not only reduces the crys-tallinity of PEO,but also promotes the dissociation of lithium salts and releases more Li^(+)ions through its interaction with the Lewis acid-base of anions,thereby overall improving the transport of lithium ***,the optimized CPEs present high ionic conductivity of 1.38×10^(−4)S/cm at 30℃,signifi-cantly improved mechanical strength(8.5 MPa),remarkable thermal stability(without obvious shrinkage at 150℃),and conspicuous Li dendrites blocking ability(>1800 h).The CPEs also both have good com-patibility and cyclic stability with LiFePO_(4)(>2000 cycles)and high-voltage LiNi_(0.8)Mn_(0.1)Co_(0.1)O_(2)(NMC811)(>500 cycles)*** addition,even at low temperature(40℃),the assembled LiFePO4/CPEs/Li bat-tery still can cycle *** novel design can provide an effective way to exploit high-performance solid-state electrolytes.

关键词： Composite polymer electrolytes Core-shell structured nanofiber Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)ceramic nanoparticle All-solid-state lithium metal batteries Outstanding thermal stability and electrochemical performance

Unusual substructure conformations observed in crystal structures of a dicistrovirus RNA-dependent RNA polymerase suggest contribution of the N-terminal extension in proper folding

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Virologica Sinica 2023年第4期38卷 531-540页

作者： Xiang Fang Guoliang lu Yanchun deng Sa Yang Chunsheng Hou Peng Gong Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan430207China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha410205China University of Chinese Academy of Sciences Beijing100049China Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing100193China Hubei Jiangxia Laboratory Wuhan430207China

The Dicistroviridae is a virus family that includes many insect *** viruses contain a positive-sense RNA genome that is replicated by the virally encoded RNA-dependent RNA polymerase(RdRP)also named 3D^(pol).Compared with the Picornaviridae RdRPs such as poliovirus(PV)3D^(pol),the Dicistroviridae representative Israeli acute paralysis virus(IAPV)3D^(pol) has an additional N-terminal extension(NE)region that is about 40-residue in *** date,both the structure and catalytic mechanism of the Dicistroviridae RdRP have remain *** we reported crystal structures of two truncated forms of IAPV 3D^(pol),namelyΔ85 andΔ40,both missing the NE region,and the 3D^(pol) protein in these structures exhibited three conformational *** palm and thumb domains of these IAPV 3D^(pol) structures are largely consistent with those of the PV 3D^(pol) ***,in all structures,the RdRP fingers domain is partially disordered,while different conformations of RdRP substructures and interactions between them are also *** particular,a large-scale conformational change occurred in the motif B-middle finger region in one protein chain of theΔ40 structure,while a previously documented alternative conformation of motif A was observed in all IAPV *** experimental data on one hand show intrinsic conformational variances of RdRP substructures,and on the other hand suggest possible contribution of the NE region in proper RdRP folding in IAPV.

关键词： Israeli acute paralysis virus(IAPV) Poliovirus RNA-dependent RNA polymerase(RdRP) Crystal structure Catalytic motif

A method for estimating masses of W Ursae Majoris-type binaries

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Research in Astronomy and Astrophysics 2020年第12期20卷 243-253页

作者： Li-Na lu Jin-Zhong Liu deng-Kai Jiang Ya-Hui Wang Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China University of Chinese Academy of Sciences Beijing 100049China Yunnan Observatories Chinese Academy of SciencesKunming 650216China Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy of SciencesKunming 650216China Laboratory of New Energy and Materials Xinjiang Institute of EngineeringUrumqi 830091China

Masses of W Ursae Majoris-type(W UMa) binaries play a critical role in investigating stellar dynamical evolutionary status. In this paper, we combine the PARSEC(PAdova and TRieste Stellar Evolution Code) with the Roche geometric model to provide a method to determine the masses of W UMa systems. To verify the validity of this method, we compile a sample of 140 spectrum binaries from the literature, which includes 76 W-and 64 A-subtype systems with reliable physical parameters. We find that the average fractional difference and the standard deviation(σM) of the residuals for W-subtype and Asubtype approximately amount to 15.66% and 0.1218, 16.03% and 0.2094, respectively. Meanwhile, we also perform detailed analyses in accordance with the orbital period, the effective temperatures and the mass ratio. We find that the method is more applicable to determine masses for W UMa systems with low effective temperature and short period.

关键词： stars:binaries:eclipsing W Ursae Majoris(W UMa)binaries masses