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The 3～(rd) International Conference on Natural Products :Natural Products -a Must for Human Survival ABSTRACTS

作者： Franoise Guéritte Institute de Chimie des Substances Naturelles CNRS 91198 Gif-sur-Yvette

Cytotoxic compounds are essential during finding new leads of anti-tumor drugs. Recently, a cytotoxic natural ester, Sintenin (1), possessing selective cytotoxicity with an ED50 value of 0.21 μg/ml to P-388 cells was isolated from the leaves and stems of Piper sintenense scattered in *** on our interests of searching for cytotoxic natural products and their derivatives, we have designed a convenient synthetic route with a total yield of 49% (4 steps) to prepare this natural ester and six of its analogues were also prepared. Furthermore, to check the preliminary structure-activity relationship (SAR) of this type of esters, we have also prepared its another seven analogues owing unsaturated double bonds conjugated to the aromatic rings, while possesing some different substituents on the aromatic *** of the synthetic compounds were passed through the cytotoxicity screening on human tumor cell lines such as PC-3, Hela, A549, BEL7404, CNE and KB. Some of the esters exhibited moderate inhibitory effects on these tumor cell lines. The results provided a preliminary knowledgement for further SAR investigation on this type of natural-based esters.

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Cloning and Characterization of Eukaryotic Translation Initiation Factor 4E (eIF4E) Gene Family in Ipomoea batatas L. (Lam) for Understanding Hexaploid Sweetpotato-Virus Interactions

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American Journal of Molecular Biology 2022年第4期12卷 203-244页

作者： Adrianne P. A. Brown Marceline Egnin Foaziatu Bukari Osagie Idehen Inocent ritte.Desmond Mortley Gregory Bernard Deloris Alexander Conrad Bonsi Plant Biotech and Genomics Research Lab Department of Agriculture and Environmental Sciences College of Agriculture Environment and Nutrition Sciences (CAES) Tuskegee University Tuskegee USA Integrative Biosciences PhD Program Tuskegee University Tuskegee USA Heliae Development LLC Gilbert USA

Characterization of genes related to sweetpotato viral disease resistance is critical for understanding plant-pathogen interactions, especially with feathery mottle virus infection. For example, genes encoding eukaryotic translation initiation factor (eIF)4E, its isoforms, eIF(iso)4E, and the cap-binding protein (CBP) in plants, have been implicated in viral infections aside from their importance in protein synthesis. Full-length cDNA encoding these putative eIF targets from susceptible/resistant and unknown hexaploid sweetpotato (Ipomoea batatas L. Lam) were amplified based on primers designed from the diploid wild-type relative Ipomoea trifida consensus sequences, and designated IbeIF4E, IbeIF(iso)4E and IbCBP. Comparative analyses following direct-sequencing of PCR-amplified cDNAs versus the cloned cDNA sequences identified multiple homeoalleles: one to four IbeIF4E, two to three IbeIF(iso)4E, and two IbCBP within all cultivars tested. Open reading frames were in the length of 696 bp IbeIF4E, 606 bp IbeIF(iso)4E, and 675 bp IbCBP. The encoded single polypeptide lengths were 232, 202, and 225 amino acids for IbeIF4E, IbeIF(iso)4E, and IbCBP, with a calculated protein molecular mass of 26 kDa, 22.8 kDa, and 25.8 kDa, while their theoretical isoelectric points were 5.1, 5.57, and 6.6, respectively. Although the homeoalleles had similar sequence lengths, single nucleotide polymorphisms and multi-allelic variations were detected within the coding sequences. The multi-sequence alignment performed revealed a 66.9% - 96.7% sequence similarity between the predicted amino acid sequences obtained from the homeoalleles and closely related species. Furthermore, phylogenetic analysis revealed ancestral relationships between the eIF4E homeoalleles and other species. The outcome herein on the eIF4E superfamily and its correlation in sequence variations suggest opportunities to decipher the role of eIF4E in hexaploid sweetpotato feathery mottle virus infection.

关键词： Ipomoea batatas Eukaryotic Translation Initiation Factors eIF4E CBP eIF(iso)4E Sweetpotato Viral Diseases

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Cloning and Characterization of Eukaryotic Translation Initiation Factor 4E (eIF4E) Gene Family in Ipomoea batatas L. (Lam) for Understanding Hexaploid Sweetpotato-Virus Interactions

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American Journal of Molecular Biology（美国分子生物学期刊） 2022年第4期

作者： adrianne p. a. brown marceline egnin foaziatu bukari osagie idehen inocent ritte.desmond mortley gregory bernard deloris alexander conrad bonsi

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