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Prediction of effective RNA interference targets and pathway-related genes in lepidopteran insects by RNA sequencing analysis

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Insect Science 2018年第3期25卷 356-367页

作者： Ruo-Bing Guan hai-Chao Li Xue-xia Miao Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

When using RNA interference （RNAi） to study gene functions in Lepidoptera insects, we discovered that some genes could not be suppressed; instead, their expression levels could be up-regulated by double-stranded RNA （dsRNA）. To predict which genes could be easily silenced, we treated the Asian corn borer （Ostriniafilrnacalis） with dsGFP （green fluorescent protein） and dsMLP （muscle lim protein）. A transcriptome sequence analysis was conducted using the cDNAs 6 h after treatment with dsRNA. The results indicated that 160 genes were up-regulated and 44 genes were down-regulated by the two dsRNAs. Then, 50 co-up-regulated, 25 co-down-regulated and 43 unaffected genes were selected to determine their RNAi responses. All the 25 down-regulated genes were knocked down by their corresponding dsRNA. However, several of the up-regulated and unaffected genes were up-regulated when treated with their corresponding dsRNAs instead of being knocked down. The genes up-regulated by the dsGFP treatment may be involved in insect immune responses or the RNAi pathway. When the immune-related genes were excluded, only seven genes were induced by dsGFP, including ago-2 and dicer-2. These results not only provide a reference for efficient RNAi target predications, but also provide some potential RNAi pathway-related genes for further study.

关键词： Ostrinia furnacalis RNA interference RNAi targets RNA-seq

Predicting ^(28)Si projectile fragmentation cross sections with Bayesian neural network method

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Chinese Physics C 2024年第12期48卷 129-137页

作者： Ying-Hua dang Jun-Sheng Li Dong-hai Zhang Institute of Modern Physics Shanxi Normal UniversityTaiyuan 030031China Shanxi University of Electronic Science and Technology Linfen 041000China

This study utilizes the Bayesian neural network(BNN)method in machine learning to learn and predict the cross-sectional data of ^(28)Si projectile fragmentation for different targets at different energies and to quantify the *** detailed modeling process of the BNN is presented,and its prediction results are compared with those of the Cummings,Nilsen,EPAX2,EPAX3,and FRACS models and experimental measurement *** results reveal that,compared with other models,the BNN method achieves the smallest root-mean-square error(RMSE)and the highest agreement with the experimental *** the BNN method and FRACS model show a significant odd-even staggering effect;however,the results of the BNN method are closer to the experimental ***,the BNN method is the only model capable of reproducing data features with low cross-section values at Z=9,and the average ratio of the predicted to experimental values of the BNN is close to *** results indicate that the BNN method can accurately reproduce and predict the fragment production cross sections of ^(28)Si projectile fragmentation and demonstrate its ability to capture key data characteristics.

关键词： Bayesian neural network(BNN) heavy ion collision projectile fragmentation cross section

Immunoglobulin G4-related lymph node disease with an orbital mass mimicking Castleman disease:A case report

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World Journal of Clinical Cases 2021年第35期9卷 10999-11006页

作者： Feng-Yun Hao Feng-xia Yang hai-Yan Bian xia Zhao Department of Pathology The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Department of Abdominal Ultrasound The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Department of Hematology The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina

BACKGROUND Immunoglobulin(Ig)G4-associated diseases are a group of systemic diseases involving multiple organs and are also known as IgG4-associated sclerosing ***4-associated lymphadenopathy occurring in the lymph nodes is characterized by a lack of specificity due to its clinicopathological characteristics and must be differentiated from a variety of lesions,such as Castleman disease,lymphatic follicular reactive hyperplasia,and *** SUMMARY A 65-year-old male patient,with Guillain-Barre syndrome for 5 years,presented to our hospital complaining of bilateral orbital mass for 2 *** hospitalization,the results of the patient’s laboratory tests showed that immunoglobulin subgroup IgG4 was 33.90 g/L and IgG was 30.30 g/L,but serum interleukin-6 was *** pathological morphology of orbital mass and cervical lymph node were consistent,which showed that a large number of plasma cells and eosinophils were observed in the lymphatic follicles,and the interstitial fibrous tissue was *** showed that CD20(B cells)(+),CD3(T cells)(+),CD38(+),IgG(+),IgG4 positive cells>100/high powered field,and IgG4/IgG>40%.Combined with clinical and immunohistochemical results,lymphadenopathy was consistent with Castleman disease-like IgG4-associated sclerosing *** acetate treatment was given at 40 mg/*** 2 wk,the superficial lymph nodes and orbital masses shrank,and the IgG4 level *** prednisone acetate was regularly used at a reduced dosage,no recurrence of the disease has been *** This case suggested that it is necessary to proceed cautiously in clinical practice with such patients,and immunoglobulin,complement,interleukin-6,C-reactive protein,and other examinations should be performed to confirm the diagnosis.

关键词： IgG4-associated disease Castleman disease Lymphadenopathy Orbital neoplasm Pathological morphology Case report

Liver injury in COVID-19: Known and unknown

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World Journal of Clinical Cases 2021年第19期9卷 4980-4989页

作者： Feng Zhou Jian xia hai-xia Yuan Ying Sun Ying Zhang Department of Endocrinology Puren Hospital of Wuhan University of Science and TechnologyWuhan 430080Hubei ProvinceChina Department of Critical Care Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina

Since the first report of the coronavirus disease 2019(COVID-19)in December 2019 in Wuhan,China,the outbreak of the disease is currently continuously *** studies have shown varying degrees of liver damage in patients with ***,the exact causes of liver injury and the relationship between COVID-19 and liver injury is *** article describes liver injury induced by COVID-19,analyzes its causes,and discusses the treatment and prognosis of liver damage in patients with COVID-19.

关键词： SARS-CoV-2 COVID-19 Liver injury Prognosis Treatment

Estimation of Critical Temperature of Thermal Explosion for Trinitromethyl Explosives by Non-isothermal DSC

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Chemical Research in Chinese Universities 2010年第3期26卷 436-442页

作者： ZHANG hai HU Rong-zu ZHAO Feng-qi GAO Hong-xu MA hai-xia Department of Mathematics Institute of Data Analysis and Computation Chemistry Northwest University Xi ＇an 710069 P. R. China Xi ＇an Modern Chemistry Researeh Institute Xi ＇an 710065 P. R. China College of Chemical Engineering Northwest University Xi ＇an 710069 P. R. China Institute of System and Information Science Xi＇an Jiaotong University Xi ＇an 710049 P. R. China

Two general expressions and their six derived formulae for estimating the critical temperature（Tb） of thermal explosion for energetic materials（EMs） were derived from the Semenov＇s thermal explosion theory and eight non-isothermal kinetic equations via reasonable hypothesis. We can easily obtain the values of the initial temperature（T0i） at which DSC curve deviates from the baseline of the non-isothermal DSC curve of EMs, the onset temperature（Tei）, the exothermic decomposition reaction kinetic parameters and the values of Too and Te0 from the equation Toiorei=Tooore0＋α1β1＋α2β2＋...＋αL-2βi^L-2, i=1, 2, …, L and then calculate the values of Tb by means of the six derived formulae. The results obtained with the six derived calculating methods for six trinitromethyl explosives： bis（2,2,2- trinitroethyl-N-nitro） ethylene diamine（BTNEDA）, 2,2,2-trinitroethyl-4,4,4-trinitrobutyrate（TNETB）, bis（2,2,2- trinitroethyl） formal（BNTF）, bis（2,2,2-trinitroethyl-nitramine）（BTNNA）, 2,2,2-trinitroethyl-2,2,2-trinitroethyl-N- nitroamino acetate（TNTNNA） and tetrakis [2,2,2-trinitroethyl] orthoester（TTNOE） agree well with each other.

关键词： Trinitromethyl explosive Critical temperature Thermal explosion Non-isothermal DSC

Numerical analysis of hydraulic fracture propagation in deep shale reservoir with different injection strategies

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第9期16卷 3558-3574页

作者： Yingjie xia Mingyu Yao Tianjiao Li hai Yang Chun'an Tang School of Civil Engineering Dalian University of TechnologyDalian116024China Deep Underground Engineering Research Center Dalian University of TechnologyDalian116024China CNPC Chuanqing Drilling Engineering Company Limited Chengdu610051China

Deep shale reservoirs are characterized by elevated breakdown pressures,diminished fracture complexity,and reduced modified volumes compared to medium and shallow ***,it is urgent to investigate particular injection strategies that can optimize breakdown pressure and fracturing efficiency to address the increasing demands for deep shale reservoir *** this study,the efficiency of various stimulation strategies,including multi-cluster simultaneous fracturing,modified alternating fracturing,alternating shut-in fracturing,and cyclic alternating fracturing,was ***,the sensitivity of factors such as the cycle index,shut-in time,cluster spacing,and horizontal permeability was ***,the flow distribution effect within the wellbore was *** results indicate that relative to multi-cluster simultaneous fracturing,modified alternating fracturing exhibits reduced susceptibility to the stress shadow effect,which results in earlier breakdown,extended hydraulic fracture lengths,and more consistent propagation despite an increase in breakdown *** alternating shut-in fracturing benefits the increase of fracture length,which is closely related to the shut-in ***,cyclic alternating fracturing markedly lowers breakdown pressure and contributes to uniform fracture propagation,in which the cycle count plays an important *** alternating fracturing demonstrates insensitivity to variations in cluster spacing,whereas horizontal permeability is a critical factor affecting fracture *** wellbore effect restrains the accumulation of pressure and flow near the perforation,delaying the initiation of hydraulic *** simulation results can provide valuable numerical insights for optimizing injection strategies for deep shale hydraulic fracturing.

关键词： Fracture propagation Numerical simulation Hydraulic fracturing Rock failure process analysis(RFPA) Injection strategy

Mechanisms for non-ideal flow in low-power arc-heated supersonic nozzles

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Acta Mechanica Sinica 2015年第4期31卷 500-511页

作者： Cheng-Kang Wu Wen-xia Pan xian Meng hai-Xing Wang State Key Laboratory of High Temperature Gas Dynamics Institute of Mechanics Chinese Academy of Sciences School of Astronautics Beijing University of Aeronautics and Astronautics

The flow in a low-powered arc gas heater com- bined with a supersonic nozzle of throat diameter less than 1 mm is quite complicated and difficult to describe in quan- titative detail. Experiments on arc-heated supersonic jet thrusters of monatomic gases argon and helium have been carried out and their performance measured. The flow charac- teristics are analyzed with the help of numerical simulation. Results show that the viscous effect is the most important factor causing the large difference between ideal and real performance. A large outer section of the exit flow is slow- moving. This is especially pronounced in helium, where 70 % of the exit area of the nozzle might be in subsonic flow. Fric- tion forces can be much larger than the net thrust, reaching several times higher in helium, resulting in very low efficien- cies. Other factors causing the differences between ideal and real flow include： complex flow in the throat region, electric arc extending to the nozzle expansion section, heat transfer to the inlet gas and from the hot plasma, and environmen- tal pressure in the vacuum chamber. It is recognized that the ordinary concepts of supersonic nozzle flow must be greatly modified when dealing with such complicated situations. The general concepts presented in this paper could be helpful in guiding the design and operation of this equipment.

关键词： Arc-heated supersonic jet Low-power Real flow Viscosity effect Low Reynolds number

Near-wellbore fracture initiation and propagation induced by drilling fluid invasion during solid fluidization mining of submarine nature gas hydrate sediments

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Petroleum Science 2021年第6期18卷 1739-1752页

作者： hai-Yan Zhu Yi-Ke dang Guo-Rong Wang Shou-Wei Zhou Qiang Fu State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of TechnologyChengdu610059SichuanChina School of Human Settlements and Civil Engineering Xi'an Jiaotong UniversityXi'an710049ShaanxiChina School of Mechatronic Engineering Southwest Petroleum UniversityChengdu610500SichuanChina China National Offshore Oil Corporation Beijing100010China

Drilling in a natural gas hydrate formation is challenging due to the poor consolidation of the formation and the potential evaporation of the *** unreasonable down-hole pressure of the drilling fluid can not only lead to the wellbore instability,but also change the predrilling condition of the natural gas hydrate formation,thus leading to an instable *** this paper,the integrated discrete element method(DEM)-computational fluid dynamics(CFD)work flow is developed to study the wellbore instability due to the penetration of the drilling fluid into the hydrate formation during crack *** results show that the difference between in-situ stresses and overpressure directly affect the drilling fluid invasion *** lower hydrate saturation leads to an easier generation of drilling fluid flow channels and the lower formation breakdown *** breakdown pressure increases with the increase of hydrate saturation,this also indicates that hydrates can enhance the mechanical properties of the *** induced cracks are initially accompanied with higher pressure of the drilling *** to the rose diagram of the fracture orientation,a wider orientation of the fracture distribution is observed at higher pressure of the invasion fluid.

关键词： Near wellbore Crack initiation and propagation Drilling fluid invasion Natural gas hydrate Wellbore instability

Impact of wetting-drying cycles and acidic conditions on the soil aggregate stability of yellow‒brown soil

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Journal of Mountain Science 2024年第6期21卷 2075-2090页

作者： xia Zhenyao NI Yuanzhen LIU Deyu WANG Di xiaO hai Key Laboratory of Geological Hazards on Three Gorges Reservoir Area Ministry of EducationYichang 443002China College of Civil Engineering&Architecture China Three Gorges UniversityYichang 443002China Engineering Research Center of Eco-environment in Three Gorges Reservoir Region Ministry of EducationChina Three Gorges UniversityYichang 443002China

Soil aggregate is the basic structural unit of soil,which is the foundation for supporting ecosystem functions,while its composition and stability is significantly affected by the external *** study was conducted to explore the effect of external environment(wetting-drying cycles and acidic conditions)on the soil aggregate distribution and stability and identify the key soil physicochemical factors that affect the soil aggregate *** yellow‒brown soil from the Three Gorges Reservoir area(TGRA)was used,and 8 wetting-drying conditions(0,1,2,3,4,5,10 and 15 cycles)were simulated under 4 acidic conditions(pH=3,4,5 and 7).The particle size distribution and soil aggregate stability were determined by wet sieving method,the contribution of environmental factors(acid condition,wetting-drying cycle and their combined action)to the soil aggregate stability was clarified and the key soil physicochemical factors that affect the soil aggregate stability under wetting-drying cycles and acidic conditions were determined by using the Pearson’s correlation analysis,Partial least squares path modeling(PLS‒PM)and multiple linear regression *** results indicate that wetting-drying cycles and acidic conditions have significant effects on the stability of soil aggregates,the soil aggregate stability gradually decreases with increasing number of wetting-drying cycles and it obviously decreases with the increase of ***,the combination of wetting-drying cycles and acidic conditions aggravate the reduction in the soil aggregate *** wetting-drying cycles,acidic conditions and their combined effect imposes significant impact on the soil aggregate stability,and the wetting-drying cycles exert the greatest *** soil aggregate stability is significantly correlated with the pH,Ca^(2+),Mg^(2+),maximum disintegration index(MDI)and soil bulk density(SBD).The PLS‒PM and multiple linear regression analysis further reveal that the soil aggre

关键词： Yellow‒brown soil Wetting-drying cycles Acidic conditions Soil aggregate stability Soil disintegration