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aBIOTECH 2024年第2期5卷 219-224页

作者： Yun Zhang Miao-Miao Cui Run-Nan Ke Yue-dan chen Kabin Xie National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Hubei Key Laboratory of Plant Pathology Huazhong Agricultural UniversityWuhan 430070China

Loss-of-function mutants are fundamental resources for gene function ***,it is difficult to generate viable and heritable knockout mutants for essential ***,we show that targeted editing of the C-terminal sequence of the embryo lethal gene MITOGEN-ACTIVATED PROTEIN KINASES 1(OsMPK1)results in weak *** C-terminal-edited osmpk1 mutants displayed severe developmental defects and altered disease resistance but generated tens of viable seeds that inherited the *** the same C-terminal editing approach,we also obtained viable mutants for a wallassociated protein kinase(Os07g0493200)and a leucine-rich repeat receptor-like protein kinase(Os01g0239700),while the null mutations of these genes were *** data suggest that protein kinase activity could be reduced by introducing frameshift mutations adjacent to the C-terminus,which could generate valuable resources for gene function studies and tune protein kinase activity for signaling pathway engineering.

关键词： CRISPR/Cas9 Lethal mutant Protein kinases Genome editing C-terminus

Engineering disease-resistant plants with alternative translation efficiency by switching uORF types through CRISPR

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Science China(Life Sciences) 2024年第8期67卷 1715-1726页

作者： Jingjing Tian Zhijuan Tang Ruixia Niu Yulu Zhou dan Yang dan chen Ming Luo Rui Mou Meng Yuan Guoyong Xu National Key Laboratory of Crop Genetic Improvement National Centre of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Hybrid Rice Institute for Advanced Studies(IAS)Wuhan UniversityWuhan 430072China Hubei Hongshan Laboratory Wuhan 430070China

Engineering disease-resistant plants can be a powerful solution to the issue of food ***,it requires addressing two fundamental questions:what genes to express and how to control their *** find a solution,we screen CRISPR-edited upstream open reading frame(uORF)variants in rice,aiming to optimize translational control of disease-related *** switching uORF types of the 5′-leader from Arabidopsis TBF1,we modulate the ribosome accessibility to the downstream firefly *** assume that by switching uORF types using CRISPR,we could generate uORF variants with alternative translation efficiency(CRISPR-aTrE-uORF).These variants,capable of boosting translation for resistance-associated genes and dampening it for susceptible ones,can help pinpoint previously unidentified genes with optimal expression *** test the assumption,we screened edited uORF variants and found that enhanced translational suppression of the plastic glutamine synthetase 2 can provide broad-spectrum disease resistance in rice with minimal fitness *** strategy,which involves modifying uORFs from none to some,or from some to none or different ones,demonstrates how translational agriculture can speed up the development of disease-resistant *** is vital for tackling the food security challenges we face due to growing populations and changing climates.

关键词： disease-resistant plants broad-spectrum disease resistance uORF uORF types uORF variants CRISPR

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980 nm Near-Infrared Light-Emitting Diode Using All-Inorganic Perovskite Nanocrystals Doped with Ytterbium Ions

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Tsinghua Science and Technology 2024年第1期29卷 207-215页

作者： Zhenglan Ye Taoran Liu dan chen Yazhou Yang Jiayi Li Yaqing Pang Xiangquan Liu Yuhua Zuo Jun Zheng Zhi Liu Buwen cheng State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Intelligent Network Research Institute Zhejiang LabHangzhou 311100China

All-inorganic perovskite(CsPbX3)nanocrystals(NCs)have recently been widely investigated as versatile solution-processable light-emitting *** to its wide-bandgap nature,the all-inorganic perovskite NC Light-Emitting Diode(LED)is limited to the visible region(400-700 nm).A particularly difficult challenge lies in the practical application of perovskite NCs in the infrared-spectrum *** this work,a 980 nm NIR all-inorganic perovskite NC LED is demonstrated,which is based on an efficient energy transfer from wide-bandgap materials(CsPbCl3 NCs)to ytterbium ions(Yb3+)as an NIR emitter doped in perovskite *** optimized CsPbCl3 NC with 15 mol%Yb3+doping concentration has the strongest 980 nm photoluminescence(PL)peak,with a PL quantum yield of 63%.An inverted perovskite NC LED is fabricated with the structure of ITO/PEDOT:PSS/poly-TPD/CsPbCl3:15 mol%Yb3+NCs/TPBi/LiF/*** LED has an External Quantum Efficiency(EQE)of 0.2%,a Full Width at Half Maximum(FWHM)of 47 nm,and a maximum luminescence of 182 cd/m?.The introduction of Yb3+doping in perovskite NCs makes it possible to expand its working wavelength to near-infrared band for next-generation light sources and shows potential applications for optoelectronic integration.

关键词： perovskite nanocrystals rare-earth doping Light-Emitting Diode(LED) near-infrared optical interconnection

Utility of real-time 3D visualization system in the early stage of phacoemulsification training

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 577-582页

作者： Zhe Xu dan chen Jing-Wei Xu Yi-Xuan Feng Ce Shi Li Zhang Wen Xu Eye Center the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009Zhejiang ProvinceChina Zhejiang Provincial Key Laboratory of Ophthalmology Hangzhou 310009Zhejiang ProvinceChina Zhejiang Provincial Clinical Research Center for Eye Diseases Hangzhou 310009Zhejiang ProvinceChina Zhejiang Provincial Engineering Institute on Eye Diseases Hangzhou 310009Zhejiang ProvinceChina

●AIM:To determine the teaching effects of a real-time three dimensional(3D)visualization system in the operating room for early-stage phacoemulsification training.●METHODS:A total of 10 ophthalmology residents of the first-year postgraduate were *** the residents were novices to cataract ***-time cataract surgical observations were performed using a custom-built 3D visualization *** training lasted 4wk(32h)in all.A modified International Council of Ophthalmology’s Ophthalmology Surgical Competency Assessment Rubric(ICO-OSCAR)containing 4 specific steps of cataract surgery was *** self-assessment(self)and expert-assessment(expert)were performed through the microsurgical attempts in the wet lab for each participant.●RESULTS:Compared with pre-training assessments(self 3.2±0.8,expert 2.5±0.6),the overall mean scores of posttraining(self 5.2±0.4,expert 4.7±0.6)were significantly improved after real-time observation training of 3D visualization system(P<0.05).Scores of 4 surgical items were significantly improved both self and expert assessment after training(P<0.05).●CONCLUSION:The 3D observation training provides novice ophthalmic residents with a better understanding of intraocular microsurgical *** is a useful tool to improve teaching efficiency of surgical education.

关键词： 3D visualization system phacoemulsification training wet lab

Research and Application of Joint Interpretation Using P-Wave and PS-Wave in Tight Gas Exploration

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Journal of Earth Science 2024年第4期35卷 1402-1406页

作者： Dong Wang Meng Zhang Jing Wang dan chen Yao Zhao Jing Bai BGPInc. China National Petroleum CorporationZhuozhou 072751China Institute of Geophysics Chengdu University of TechnologyChengdu 610059China National Engineering Research Center of Oil and Gas Exploration Computer Software Zhuozhou 072751China

The exploration and development of tight sandstone gas reservoirs are controlled by high-quality river channel sand bodies on a large scale in Sichuan *** order to improve the accu-racy of sand body prediction and characterization,Multi-component exploration technology research has been carried out in Northwest Sichuan ***,based on the array acoustic logging data,a for-ward modeling has been established to analyze the seismic response characteristics of the PS-wave data and P-wave *** result shows that the response characteristics of the P-wave and PS-wave to the sand bodies with different impedance are *** then through the analysis of logging data,the effectiveness of the forward modeling has been *** the sandstone velocity is close to the sur-rounding rocks,the P-wave performs as a weak reflection,which may lead to reduce the identification range of the sand ***,the PS-wave exhibits strong reflection,which can identify this type of sand ***,by comparing and explaining the PS-wave data and P-wave data,and integrat-ing their attributes,the prediction accuracy of sand bodies is *** with the interpreta-tion of a single P-wave,the results can significantly expand the distribution range of sand bodies,lay-ing a foundation for improving the production capacity of single wells and reserve submission.

关键词： multi-component exploration tight gas sand body identification

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“壮族三月三”的育人意蕴与创新路向

广西民族研究 2024年第1期 114-121页

作者：陈丹浙江外国语学院教育学院/教育治理研究中心杭州310023

发挥民族节日仪式的教化功能是中华文明历史演进中的文化传统。“壮族三月三”是广西历史最悠久、参与人数最多、传播面最广的全国性节日。其作为中华上巳节的现代传承,是中华传统文化的个性表达,也是展示中国形象的独特视角。其具有以... 详细信息

发挥民族节日仪式的教化功能是中华文明历史演进中的文化传统。“壮族三月三”是广西历史最悠久、参与人数最多、传播面最广的全国性节日。其作为中华上巳节的现代传承,是中华传统文化的个性表达,也是展示中国形象的独特视角。其具有以文化认同增进民族团结、以文化习俗促进民族繁荣、以文化传承推进民族发展、以文化育人提升个体成长的育人意蕴。在新时代背景下,铸牢中华民族共同体意识、政府社会学校共同参与、节日仪式融入生活常态、精神内核引领呈现方式、信息技术拓展传播领域等创新路向,对于维护壮族文化生态体系、传播中华优秀传统文化具有重要的现实意义。

关键词：壮族三月三文化渊源育人意蕴创新路向

“圣物之灵”的消解与流转:潮汕弃置神像的生命史

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原生态民族文化学刊 2024年第2期16卷 141-152,156页

作者：陈丹广州美术学院广州510000

作为圣物的神像可能面临被弃置、更换的命运。既往对弃置神像的研究聚焦于其仪式仪轨及家庭生活中的弃像行为,缺乏对不同层面的行动者如何参与其中、“圣物之灵”如何运作的追踪。在潮汕乡村,公庙、家庭、造像师等都可能弃置、更换神像... 详细信息

作为圣物的神像可能面临被弃置、更换的命运。既往对弃置神像的研究聚焦于其仪式仪轨及家庭生活中的弃像行为,缺乏对不同层面的行动者如何参与其中、“圣物之灵”如何运作的追踪。在潮汕乡村,公庙、家庭、造像师等都可能弃置、更换神像。在村落集体中,由公庙组织的神像废弃、更新仪式,促成社会秩序的重整与灵的流转;在家庭关系中,神像与祀奉者的生命历程相互纠缠,致使弃像之“灵”退而不散;在生产委托关系中,累代师传的造像师之“神性”与拥有神形神名的神像之“物性”勾连,诱导灵不请自来。因而,神像既为表征意义上的神,也是与相关行动者之身份、体验、情感、经历等缠绕的实践中的物。神像之“灵”的流动生发于表征系统与具体实践相互作用的过程。

关键词：物的社会生命弃像灵圣物

RBM25 depletion suppresses the growth of colon cancer cells through regulating alternative splicing of MNK2

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Science China Life Sciences 2024年

作者： Lili Zhi Chaoqun chen Ge Zhang Tian Huang Wenxia He Jinrui Zhang dan chen Jiayi Liu Jinyao Zhao Yangfan Qi Guiying Wang Wenjing Zhang Yang Wang Sino-US Research Center for Cancer Translational Medicine of the Second Affiliated Hospital of Dalian Medical University&Institute of Cancer Stem Cell Dalian Medical University Department of Immunology College of Basic Medical SciencesDalian Medical University Institute of Cancer Stem Cell Dalian Medical University Department of Pathology the First Affiliated Hospital of Dalian Medical UniversityDalian Medical University Department of General Surgery the Second Hospital of Hebei Medical University The Second Department of General Surgery the Fourth Hospital of Hebei Medical University Hebei Key Laboratory of Etiology Tracing and Individualized Diagnosis and Treatment for Digestive System Carcinoma

Increasing evidence suggests that deregulated RNA splicing factors play critical roles in tumorigenesis;however,their specific involvement in colon cancer remains largely *** we report that the splicing factor RBM25 is overexpressed in colon cancer,and this increased expression correlates with a poor prognosis of patients with colon ***,RBM25 ablation suppresses the growth of colon cancer cells both in vitro and in ***,our transcriptome-wide analysis of splicing events revealed that RBM25regulates a large number of cancer-related alternative splicing events across the human genome in colon ***,RBM25regulates the splicing of MNK2 by interacting with the poly G rich region in exon 14a,thereby inhibiting the selection of the proximal 3′splice site (ss),resulting in the production of the oncogenic short isoform,*** of RBM25 leads to an increase in the MNK2a isoform and a decrease in the MNK2b ***,re-expression of MNK2b or blocking the 3′ss of the alternative exon 14a with ASO partially reverses the RBM25 knockdown mediated tumor ***,MNK2b levels were significantly increased in colon cancer tissues,which is positively correlated with the expression level of ***,our findings uncover the critical role of RBM25 as a key splicing factor in colon cancer,suggesting its potential as a prognostic marker and therapeutic target.

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基于SoS核函数调制的超声图像伪影剔除方法

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Journal of Southeast University(English Edition) 2024年第1期40卷 80-88页

作者：宋寿鹏贾慧陈丹江苏大学机械工程学院镇江212013

针对超声图像伪影严重的问题,提出了一种基于SoS核函数调制的超声图像伪影剔除方法,该方法能同时剔除由等声程线扩散、传感器拖尾现象和检测噪声产生的伪影.该方法利用SoS核函数对测得的超声回波信号进行调制,对调制后的信号等间隔低速... 详细信息

针对超声图像伪影严重的问题,提出了一种基于SoS核函数调制的超声图像伪影剔除方法,该方法能同时剔除由等声程线扩散、传感器拖尾现象和检测噪声产生的伪影.该方法利用SoS核函数对测得的超声回波信号进行调制,对调制后的信号等间隔低速率采样来获取离散序列值,再对离散序列进行离散傅里叶变换得到傅里叶系数序列,利用谱估计算法对回波信号参数进行估计,获取检测信号的特征参数,最后对回波信号进行自适应重构,计算声场得到超声图像.试验结果表明,聚焦和直探头对槽和通孔类缺陷的去伪影效果明显,与传统B扫图像相比,峰值信噪比分别达到了24.306、23.213、15.074和16.444 dB,结构相似度分别为0.931、0.932、0.746和0.773,表明该算法有效提高了缺陷图像的质量.

关键词：无损检测超声图像伪影 SoS核函数缺陷

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水下无线光深度自动编码器通信性能

光学学报 2024年第12期44卷 1-10页

作者：陈丹王睿艾菲尔汤林海西安理工大学自动化与信息工程学院陕西西安710048

自动编码器利用深度神经网络联合优化发射机和接收机实现端到端通信。与具有模块化结构的传统通信不同,基于深度学习的通信系统可根据不同编码方案学习最优映射空间。海洋环境中吸收、散射以及湍流效应严重影响水下无线光通信系统性能,... 详细信息

自动编码器利用深度神经网络联合优化发射机和接收机实现端到端通信。与具有模块化结构的传统通信不同,基于深度学习的通信系统可根据不同编码方案学习最优映射空间。海洋环境中吸收、散射以及湍流效应严重影响水下无线光通信系统性能,基于考虑Gamma-Gamma湍流和传输路径损耗影响的水下联合信道模型,针对自动编码器独热矢量数据传输速率有限的问题,提出一种水下自动编码器自适应传输方案,在不同海洋信道以及不同网络训练条件下根据均方误差性能约束选择最优传输矢量,实现传输速率最大化并提高通信性能。仿真结果表明自动编码器与传统通信相比可获得更优的误码率(BER)性能,且不同训练参数集合下自适应传输方案均可获得比传统独热矢量更低的BER与更高的数据速率。

关键词：水下无线光通信自动编码器自适应传输深度学习误码率