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Population differences in susceptibility to stress in Littorina saxatilis from metal contaminated and uncontaminated sites in the Isle of Man

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Journal of Environmental Sciences 2006年第2期18卷 364-368页

作者： daka erema ransome The University of Liverpool Port Erin Marine Laboratory Isle of Man IM9 6 JA United Kingdom Department of Applied and Environmental Biology Rivers S tate University of Science and Technology PMB 5080 Port Harcourt Nigeria.

The population of the intertidal gastropod Littorina saxcailis from Laxey estuary in the Isle of Man is exposed to mine-related contamination of Zn （and is tolerant to Zn and Pb） compared to those of Castletown, Derbyhaven, Peel and Ramsey. Tolerances to salinity and desiccation stress were assayed to test how metal tolerance could affect tolerance to general stress. The winkles were experimentally exposed to salinities of 0, 17 and 60 practical salinity units （psu） with 34 psu as control. Desiccation stress was determined by aerial exposure of the winkles at 24℃. No control mortalities were recorded and mortalities in 17 psu were less than 50% for all populations over a 24-day exposure period. LT50 values （mean±SD, n=5） at 0 psu ranged from 6.6 ± 0.6 to 7.5 ± 1.3 d for winkles from Ramsey and Laxey respectively. The values for 60 psu ranged from 7.0±0.7 to 8.4±0.9 d. No significant differences in LT50 values were obtained for the salinity exposurcs（ANOVA,P〉0.05） but there was a significant difference in tolerance to desiccation （P〈0.01）. The winkles from Laxey showed very high susceptibility to desiccation stress probably indicating a physiological trade-off to metal tolerance.

关键词： tolerance salinity desiccation winkles Isle of Man

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Synergistic Mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera Aqueous Extract: Its Liver and Kidney Benefits in Male Albino Rat Model

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Journal of Biosciences and Medicines 2023年第10期11卷 63-75页

作者： Tamuno-Boma Odinga Cletus Barizoge Lemii Iyaeneomi ransome daka Christine U. Gabriel-Brisibe Sarah K. Enebeli Iyingiala Austin-Asomeji Felicia Ucheawaji Edward Department of Biochemistry Faculty of Science Rivers State University Port Harcourt Nigeria Department of Pharmacology and Therapeutics Faculty of Basic Medical Sciences College of Basic Medical Science Rivers State University Port Harcourt Nigeria Department of Medical Biochemistry Faculty of Basic Medical Sciences College of Medical Science Rivers State University Port Harcourt Nigeria Department of Community Medicine Faculty of Clinical Sciences College of Medical Sciences Rivers State University Port Harcourt Nigeria

Background: The synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract is a common drink in Port Harcourt, Nigeria. It is assumed to have various health benefits. However, the synergistic mixture of the content has not been studied scientifically, hence the need to evaluate its effect on the liver and kidney being part of the body’s metabolic organs. Aim: This study evaluated the synergistic mixture of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera (STCD) aqueous extract in male albino rats. Methods: Acute toxicity LD50 of STCD was carried out, afterwards, fifteen male albino rats were grouped into three groups with 5 rats in each group;Control, 200 mg/kg, 400 mg/kg STCD. The rats were administered STCD orally 24 hourly, for 21 days, with feed and water ad libitum. At the end of the experiment, blood samples were collected for biochemical analysis of the liver and kidney biomarkers, while the liver and kidney tissues were harvested for histopathological examination using standard laboratory methods. Descriptive statistics were computed and expressed as Mean ± SD. One-way ANOVA and Turkeys test was performed. P value ≤0.05 was considered statistically significant. Results: Acute toxicity LD50 of STCD was observed to be ≥2404.2 mg/kg body weight. An increase in the percentage body weight difference of 8.39% and 2.86% was observed for 200 and 400 mg/kg STCD groups. Also, the liver weight was observed to increase in 400 mg/kg (3.92 ± 1.42) in comparison to the control group (3.48 ± 1.61), a decrease in the kidney weight was observed in all groups administered STCD in comparison to the control group. Administration of STCD at both 200 and 400 mg/kg revealed a decrease in the concentration of the hepatic biomarkers AST, ALT, ALP, TP, Albumin, Total and conjugated bilirubin. The kidney biomarker Urea was observed to decrease in concentration for 200 mg/kg STCD (4.60 ± 1.83) and 400 mg/kg STCD (4.76 ±

关键词： Cyperus esculentus (Tiger nuts) Phoenix dactylifera (Dates) and Cocos nucifera (Coconuts) Liver Kidney Male Rats

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