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Noninvasive and nondestructive sampling for avian microsatellite genotyping: a case study on the vulnerable Chinese Egret (Egretta eulophotes)

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Chinese Birds 2015年第4期6卷 276-284页

作者： yufei dai Qingxian Lin Wenzhen Fang Xiaoping Zhou Xiaolin Chen Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems School of Life SciencesCollege of the Environment and EcologyXiamen University

Background: Noninvasive and nondestructive DNA sampling techniques are becoming more important in genetic studies because they can provide genetic material from wild animals with less or even without disturbance,which is particularly useful for the study of endangered species,i.e.,***,nondestructively and noninvasively sampled DNA may,in some cases,be inadequate in the amount and quality of the material collected,which can lead to low amplification success rates and high genotyping ***: In this study,noninvasive(eggshell swab,shed feather and feces),nondestructive(plucked feather and buccal swab) and invasive(blood) DNA samples were collected from the vulnerable Chinese Egret(Egretta eulophotes).DNA concentrations,PCR amplification success and microsatellite genotyping errors of different sample types were evaluated and compared to determine whether noninvasive and nondestructive samples performed as well as invasive samples in our experimental ***: A total of 159 samples were collected in the *** the different sample types,the highest DNA concentrations(154.0–385.5 ng/μL) were obtained from *** extracted from fecal samples were the lowest,ranging from 1.25 to 27.5 ng/μ*** all of the DNA samples,i.e.,95.59 %,were successfully amplified for mt DNA(n = 152) and 92.76 % of mt DNA samples were successfully genotyped for at least five of the nine microsatellite loci tested(n = 141).Blood samples and buccal swabs produced reliable genotypes with no genotyping errors,but in feces,allelic dropouts and false alleles occurred in all nine loci,with error rates ranging from 6.67 to 38.10 % for the dropouts and from 6.06 to 15.15 % for the false ***: These results indicate that both nondestructive and noninvasive samplings are suitable for avian microsatellite genotyping,save for fecal ***,we should remain cautious of the appearance of genotyping errors,especially when using noninvasive material.

关键词： Genotyping Noninvasive sampling Nondestructive sampling Microsatellite PCR Avian genetics Chinese Egret

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An overview of cetacean strandings, bycatches and rescues along the western coast of the Taiwan Strait, China: 2010–2015

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Acta Oceanologica Sinica 2017年第12期36卷 31-36页

作者： ZHAO Liyuan ZHU Qian MIAO Xing XU Min WU Fuxing dai yufei TAO Cuihua MOU Jianfeng WANG Xianyan Laboratory of Marine Biology and Ecology Third Institute of OceanographyState Oceanic Administration Ocean College Shandong University (Weihai)

The analysis of cetacean strandings can provide fundamental information about species diversity and composition in a particular region. The present study collected and analyzed cetacean strandings, bycatches and rescues along the western coast of the Taiwan Strait, China, from 2010 to 2015. In total, 48 records, including 37 strandings, 8 bycatches and 3 rescues, involving 13 known species were collected. Among them, Indo-Pacific humpback dolphins（Sousa chinensis） and finless porpoises（Neophocaena phocaenoides） were the most common species, consisting of 31.3% and 25%, respectively. Notably, 10 out of the 48（20.8%） records were collected from the Pingtan Island and included 3 species of Mysticeti and 4 species of Odontoceti. Finally, we compared the cetacean species composition between the western and eastern coasts of the Taiwan Strait; 31 cetacean species occurred in the Taiwan Strait, indicating a relatively high cetacean diversity in this region. Systematic field surveys are urgently needed to explore the cetacean species composition, population stock and the related habitat status in the Taiwan Strait, which may improve conservation management in the future.

关键词： cetacean stranding bycatch rescue species diversity Taiwan Strait

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Utility Evaluation of HLA-B*13:01 Screening in Preventing Trichloroethylene Induced Hypersensitivity Syndrome in a Prospective Cohort Study

Utility Evaluation of HLA-B*13:01 Screening in Preventing Tr...

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中国毒理学会第九次全国毒理学大会

作者： yufei dai Chinese Center for Disease Control and Prevention National Institute of Occupational Health and Poison Control

Introduction Trichloroethylene（TCE）-induced hypersensitivity syndrome（TIHS） is a dose-independent and potentially life threatening disease. Several genetic susceptibility biomarkers have been found to associate with TIHS, however, no systematic prospective study has been conducted to evaluate the utility of these genetic susceptibility biomarkers in preventing the disease. METHODS 1764 newly hired TCE-exposed workers were recruited in this cohort study, 1651 of them went through HLA-B*13:01 genotyping and 3-month follow-up procedure. All workers were monitored for adverse reaction by telephone interview every week. The workers with early symptoms of TIHS were asked to go to the hospital immediately for further examination, diagnosis, and treatment. The medical expense record data of patients with TIHS was collected for cost-effectiveness analysis. RESULTS Among 1651 workers, 158（9.57%） were found to carry the HLA-B*13:01 allele and 16（0.97%） were diagnosed with TIHS. HLA-B*13:01 allele was significantly associated with an increased TIHS risk（RR=28.4, 95% CI=9.2～86.8, P＜0.0001）. As a risk predictor of TIHS, HLA-B*13:01 testing had a sensitivity of 75%, a specificity of91.1%, and an AUC of 0.83（95% CI=0.705-0.955）, the positive predictive value and negative predictive value were 7.6% and 99.7%, respectively. The incidence of TIHS was significantly decreased in HLA-B*13:01 non-carriers（0.27%） compared with all workers（0.97%, P=0.014）. Cost-effectiveness analysis showed that HLA-B*13:01 screening could produce an economic saving of $4604 per TIHS avoided. CONCLUSIONS Prospective HLA-B*13:01 screening may significantly reduce the incidence of TIHS and could be a cost effective option for preventing the disease in TCE-exposed workers

关键词： Trichloroethylene Hypersensitivity syndrome Prospective cohort study Cost-effectiveness analysis

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Early divergence and differential population histories of the Indo-Pacific humpback dolphin in the Pacific and Indian Oceans

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Integrative Zoology 2021年第4期16卷 612-625页

作者： Liyuan ZHAO Watchara SAKORNWIMON Wenzhi LIN Peijun ZHANG Rachawadee CHANTRA yufei dai Reyilamu AIERKEN Fuxing WU Songhai LI Kongkiat KITTIWATTANAWONG Xianyan WANG Laboratory of Marine Biology and Ecology Third Institute of OceanographyMinistry of Natural ResourcesXiamenChina Marine and Coastal Resources Research Center The Middle Gulf of ThailandChumphonThailand Institute of Deep-sea Science and Engineering Chinese Academy of SciencesSanyaChina Marine and Coastal Resources Research Center The Upper Gulf of ThailandSamut SakhonThailand Fujian Provincial Key Laboratory of Marine Ecological Conservation and Restoration XiamenChina Phuket Marine Biological Research Center PhuketThailand

The currently recognized Indo-Pacific humpback dolphin occurs in estuaries and surrounding shallow waters from the South China Sea to the Asian coast of the Indian ***,a recent study suggested that the humpback dolphin from the Bay of Bengal may represent a distinct phylogenetic *** this study,we sequenced 915-bp mtDNA segments from five geographic populations in both Chinese and Thai waters;together with previously published sequences,these data revealed that the ancestral Indo-Pacific humpback dolphin might have split during the transition from the Oligocene to Miocene(23.45 Mya,95%HPD:16.65–26.55 Mya),and then dispersed along the Pacific and Indian Ocean coasts of *** differentiation was detected between most of the examined populations,except for only a few pairwise populations in the northern South China *** differentiation/distance between the humpback dolphins from the northern and southern South China Sea met the sub-species threshold value proposed for marine mammals,whereas that between the humpback dolphins in the Pacific and the Indian Ocean was above the species *** inference of historic gene flow indicated low but constant northward gene flow along the Indian Ocean coast;however,there was a recent abrupt increase in gene flow in the Pacific region,likely due to the shortening coastline at the low stand of sea *** results revealed that the current taxonomic classification of Indo-Pacific humpback dolphins may not reflect their phylogeography.

关键词： historic gene flow Indo-Pacific region phylogenetic species concept phylogeography Sousa chinensis

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The Effect of 2,5-hexanedione on Myelin Protein Zero Expression,and Its Mitigation Using Ginkgo Biloba Extract

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Biomedical and Environmental Sciences 2011年第4期24卷 374-382页

作者： ZHAO Lei LIU QingJun CHEN Hong DUAN HuaWei BIN Ping LIU Qing NIU Yong dai yufei ZHENG YuXin National Institute for Occupational Health and Poison Control and Key laboratory of Chemical Safety and Health Chinese Center for Disease Control and Prevention Beijing 100050 China

Objective To investigate the role of myelin protein zero （P 0） in 2,5-hexanedione （2,5-HD）-induced peripheral nerve injury,and the protective effect of Ginkgo biloba extract （Egb761） on 2,5-HD-induced toxic peripheral *** After 4 weeks of treatment with 2,5-HD at different doses （50,100,200,400 mg/kg） in rats,changes in the levels of P 0 in rat sciatic nerves was investigated,and the effect of Egb761 on 2,5-HD-induced toxic peripheral neuropathy was *** The blood-nerve barrier （BNB） permeability of the sciatic nerve increased,and the expression of P 0 mRNA and P 0 protein decreased in a dose-dependent manner after treatment with 2,5-HD for 4 *** with Egb761 protected against BNB interruption,and inhibited P 0 mRNA and protein reduction during 2,5-HD *** with Egb761 significantly reduced loss of body weight （P0.01） and mitigated gait abnormalities （2.85±0.22） induced by 400 mg/kg 2,5-HD （P0.01）.It also reduced the signs of neurotoxicity induced by 2,*** 2,5-HD inhibited the expression of P 0 in a dose-dependent manner,and this may be an important mechanism by which toxic peripheral neuropathy is induced by 2,***761 has a protective effect against 2,5-HD-induced peripheral neurotoxicity in rats.

关键词： Myelin protein zero 2 5-hexanedione Ginkgo biloba extract

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Polycyclic aromatic hydrocarbons and metals co-exposure induce ribosomal DNA copy number amplification

Polycyclic aromatic hydrocarbons and metals co-exposure indu...

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中国毒理学会第十次全国毒理学大会

作者： Yanhua Wang Liya Zhang Huige Yuan Zhizhou He Yong Niu yufei dai Huawei Duan National Institute for Occupational Health and Poison Control Chinese Center for Disease Control and Prevention

Background: The ribosomal DNA(rDNA) are highly repetitive in eukaryotic genomes and plays an essential role in maintaining genome stability. So far, alterations of rDNA from airborne pollutants exposure remain unclear. Nasal epithelial cell has been an accessible surrogate for evaluating respiratory impairment from the multiple classes of pollutants in the real word. METHODS We developed a mixture-centered biomarkers study among 768 subjects from a particle emission in Eastern China. We identified the mixed exposure of PAHs and metals by environmental and biological monitoring. We selected urinary 8-hydroxy-2’-deoxyguanosine(8-OHdG) as the marker of DNA oxidative damage, collected nasal samples, and measured their rDNA copy number(rDNA CN) in our population. We used the multiple linear regression to explore the response between each exposure and rDNA CN. The adaptive elastic net(adENET) regression and bayesian kernel machine regression(BKMR) models were performed to pick out the most significant exposure factors, and assess the hybrid effect and interactions of multiple pollutants. Mediation analyses were set out to assess the roles of 8-OHdG in the associations between exposure analytes and rDNA CN. RESULTS The exposure-response between the mixed PAHs and metals emission on nasal 5S and 45S rDNA CN amplification were found. PAHs and Ni in the mixture played a substantial role in expanding nasal rDNA. In addition, the 8-OHdG partially mediated impacts of PAHs and Ni exposure on nasal rDNA CN amplification. CONCLUSION Our findings suggested that DNA oxidative stress might trigger rDNA instability induced by inhaled PAHs and metals.

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Myelin protein zero and its antibody in serum as biomarkers of n-hexane-induced peripheral neuropathy and neurotoxicity effects

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Chinese Medical Journal 2014年第8期127卷 1536-1540页

作者： Jia Xiaowei Liu Qingjun Zhang Yanshu dai yufei Duan Huawei Bin Ping Niu Yong Liu Jie Zhong Liuzhen Guo Jisheng Liu Xiaofeng Zheng Yuxin Key Laboratory of Chemical Safety and Health National Institute for Occupational Health and Poison Control Chinese Center for Disease Control and Prevention Beijing 100050 China DSM Regulatory Affairs Depart-ment DSM (China) Ltd. Beijing 100000 China College of Public Health Hebei United University Tangshan Hebei 063009 China Suzhou Fifth People's Hospital Suzhou Jiangsu 215007 China Xixiang Health Inspection of Bao'an Distract Shenzhen Guangzhou Guangdong 500000 China

Background Chronic exposure to n-hexane can lead to peripheral neuropathy that no effective treatment regimen could be applied presently. This study investigated whether myelin protein zero （P0） protein and its antibody could be used to distinguish n-hexane intoxication and protect workers from peripheral neuropathy. Methods We compared P0 protein and its antibody among three levels of n-hexane-exposed groups, which included 18 patients with n-hexane-induced peripheral neuropathy as case group, 120 n-hexane-exposed workers as n-hexane- exposed control group, and 147 non-hexane-exposed participants used as control group. ELISA method was applied to detect P0 protein and its antibody. Results P0 protein in serum was significantly higher in the case group and n-hexane-exposed control group in comparison with the control group （P〈0.01）. Compared with the n-hexane-exposed control group, the case group also had significant increase of P0 protein （P〈0.01）. After 6 months therapy, P0 protein was observed to decrease significantly in the case group （P〈0.01）. The P0 antibody in serum was significantly higher in the n-hexane-exposed control group than in the control group （P〈0.01）, but not significantly different between cases and controls. Conclusions P0 antibodies in serum may be a short-term effect biomarker for n-hexane exposure. P0 protein in serum may be an early effective biomarker for peripheral nerve neuropathy and its biological limit value needs investigation in the future study.

关键词： n-hexane marker peripheral neuropathy myelin protein zero

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Identification of the HLA-B*1301-bound peptides

Identification of the HLA-B*1301-bound peptides

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生命的分子机器及其调控网络——2012年全国生物化学与分子生物学学术大会

作者： Jun Zhang Haijun Yang Haishan Li Feng Liu Qiang Jia Huawei Duan Yong Niu Ping Bin Yuxin Zheng yufei dai Key laboratory of Chemical Safety and Health Chinese Centre for Disease Control and Prevention.National Institute for Occupational Health and Poison ControlChinese Centre for Disease Control and Prevention Department of Preventive Medicine College of Basic Medical SciencesHubei University of Chinese Medicine

Trichloroethylene(TCE) induced hypersensitivity dermatitis is one of the critical occupational disease among workers in China which is unpredictable,dose-independent and life *** mechanisms by which TCE causes the disease are still not well *** previous genetic association studies have identified a strong linkage between TCE-induced hypersensitivity dermatitis and the HLA-B*1301 *** this study,we searched for peptides bound to the HLA-B*1301 molecules that might be involved in the *** peptides were recovered by recombinant expression of HLA-B* 1301 mo-lecules from the C1R-HLA -B*1301 transfectant cells and analyzed using liquid chromato-graphy-tandem mass spectrometry.A total of 57 HLA-B*1301- bound peptides from the C1R-HLA-B*1301 transfectant cells were identified,and these peptides ranged from 9-23 amino acids(AAs) *** aforementioned peptides were mainly 9(22/57,38.60%),10(15/57,26.32%),11(13/57,22.81%) AAs long;in addition,4 peptides were 12 AAs long,and 3 peptides were each 16,20,23 AAs *** analyze the characteristics of HLA-B*1301-bound peptides,the frequency of AA residues at each position were calculated by an algorithm which originally accommodated only 9-12-mer *** these pep-tides,there was a strong preferences for AA residues L(38.89%),Q(33.33%) at the anchoring residue P2 and L(35.19%),F(24.07%),I(14.81%),W(11.11%) at the P9(Pc) ***-estingly,hydrophobic AA residues were of high specificity at Pc,accounting for 92.59%.In addition,AAs R(25.93%) and A(18.52%) were found more frequently at P1,D (29.63%) at P3,E(24.07%) at P6,and R(24.07%) and K(20.37%) at ***-B* 1301-bound peptides were identified for the first time in our research,the results of which could greatly contrib-ute to the HLA binding peptides database and enhance the mechanistic understanding of TCE-induced hypersensitivity dermatitis.

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村镇复合隔震建筑抗震性能影响因素的研究

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石河子大学学报（自然科学版） 2020年第6期38卷 691-698页

作者：王荣锦袁康代宇飞石河子大学水利建筑工程学院新疆石河子832003 石河子大学新疆兵团高烈度寒区建筑抗震节能技术工程实验室新疆石河子832003

为了研究村镇复合隔震技术相关影响因素对房屋抗震性能的影响,并明确不同烈度区合理的设计参数,本文结合村镇砌体房屋建造实际情况,采用ABAQUS有限元软件建立了复合隔震结构、传统的砌体结构2种模型,进行弹塑性动力时程分析,通过对比这... 详细信息

为了研究村镇复合隔震技术相关影响因素对房屋抗震性能的影响,并明确不同烈度区合理的设计参数,本文结合村镇砌体房屋建造实际情况,采用ABAQUS有限元软件建立了复合隔震结构、传统的砌体结构2种模型,进行弹塑性动力时程分析,通过对比这2种模型得出地震烈度、滑移层摩擦系数、房屋层数及场地类别等因素对复合隔震建筑加速度、位移、上部结构底部剪力等动力响应的影响规律。结果表明:复合隔震体系具有良好的隔震效果,其主要影响因素为地震烈度和滑移层摩擦系数;随着地震烈度的增加、摩擦系数的减小,复合隔震结构的隔震率显著增加;在预设隔震率为40%的条件下,复合隔震结构滑移层摩擦系数在不同抗震设防烈度区的合理取值范围为:7度区(0.1 g)应小于0.2,7度区(0.15 g)应小于0.3,8度区(0.2 g)应小于0.32,8度区(0.3 g)应小于0.35。

关键词：罕遇地震建筑复合隔震体系影响规律设计参数

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活性氧对血红蛋白损伤机理的研究

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卫生研究 1999年第1期28卷 30-32页

作者：戴宇飞常平李桂兰林永齐中国预防医学科学院劳动卫生与职业病研究所吉林大学分子生物学系

研究了超氧负离子（Ｏ－２）和过氧化氢（Ｈ２Ｏ２）对血红蛋白（Ｈｂ）的损伤作用。结果表明二者对Ｈｂ的损伤主要集中在血红素辅基部分，但二者损伤途径不同，Ｏ－２是通过电荷中和破坏了辅基与其周围氨基酸残基之间的静电相互作用，... 详细信息

研究了超氧负离子（Ｏ－２）和过氧化氢（Ｈ２Ｏ２）对血红蛋白（Ｈｂ）的损伤作用。结果表明二者对Ｈｂ的损伤主要集中在血红素辅基部分，但二者损伤途径不同，Ｏ－２是通过电荷中和破坏了辅基与其周围氨基酸残基之间的静电相互作用，从而使辅基完整地脱落；而Ｈ２Ｏ２则是通过Ｆｅｎｔｏｎ反应与血红素辅基中心的Ｆｅ２＋反应产生羟自由基（ＯＨ），ＯＨ在短距离内定点损伤卟啉环结构。

关键词：活性氧血红蛋白血红素

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