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Journal of Integrative Agriculture 2016年第5期15卷 1046-1058页

作者： HUANG Yao LI Ji-kun QIANG Sheng dai wei-min SONG Xiao-ling Weed Research Lab Nanjing Agricultural University

Stacked（insect and herbicide resistant） transgenic rice T1c-19 with cry1C＊/bar genes, its receptor rice minghui 63（herein MH63） and a local two-line hybrid indica rice Fengliangyou Xiang 1（used as a control） were compared for agronomic performance under field conditions without the relevant selection pressures. Agronomic traits（plant height, tiller number, and aboveground dry biomass）, reproductive ability（pollen viability, panicle length, and filled grain number of main panicles, seed set, and grain yield）, and weediness characteristics（seed shattering, seed overwintering ability, and volunteer seedling recruitment） were used to assess the potential weediness without selection pressure of stacked transgene rice T1c-19. In wet direct-seeded and transplanted rice fields, T1c-19 and its receptor MH63 performed similarly regarding vegetative growth and reproductive ability, but both of them were significantly inferior to the control. T1c-19 did not display weed characteristics; it had weak overwintering ability, low seed shattering and failed to establish volunteers. Exogenous insect and herbicide resistance genes did not confer competitive advantage to transgenic rice T1c-19 grown in the field without the relevant selection pressures.

关键词： stacked transgenic rice T1c-19 agronomic traits reproductive ability weediness

Fitness of F1 hybrids between stacked transgenic rice T1c-19 with cry1C*/bar genes and weedy rice

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Journal of Integrative Agriculture 2019年第12期18卷 2793-2805页

作者： HUANG Yao WANG Yuan-yuan QIANG Sheng SONG Xiao-ling dai wei-min Weed Research Laboratory College of Life Sciences Nanjing Agricultural University

Compared to single-trait transgenic crops, stacked transgenic plants may be more prone to become weedy, and transgene flow from stacked transgenic plants to weedy relatives may pose a potential environmental risk because these hybrids could be more advantageous under specific environmental conditions. Evaluation of the potential environmental risk caused by stacked transgenes is essential for assessing the environmental consequences caused by crop-weed transgene flow. The agronomic performance of fitness-related traits was assessed in F1+(transgene positive) hybrids(using the transgenic line T1 c-19 as the paternal parent) in monoculture and mixed planting under presence or absence glufosinate pressure in the presence or absence of natural insect pressure and then compared with the performance of F1–(transgene negative) hybrids(using the non-transgenic line minghui 63(MH63) as the paternal parent) and their weedy rice counterparts. The results demonstrated that compared with the F1– hybrids and weedy rice counterparts, the F1+ hybrid presented higher performance(P0.05) under natural insect pressure, respectively, lower performance(P0.05) in the absence of insect pressure in monoculture planting, respectively. And compared to weedy rice counterparts, the F1+ hybrid presented higher performance(P0.05) in the presence or absence of insect pressure in mixed planting, respectively. The F1+ hybrids presented nonsignificant changes(P>0.05) under the presence or absence glufosinate pressure under insect or non-insect pressure in monoculture planting. The all F1+ hybrids and two of three F1– hybrids had significantly lower(P<0.05) seed shattering than the weedy rice counterparts. The potential risk of gene flow from T1 c-19 to weedy rice should be prevented due to the greater fitness advantage of F1 hybrids in the majority of cases.

关键词： weedy rice hybrids stacked transgenes safety assessment fitness

Application of human bone marrow-derived mesenchymal stem cells in the treatment of radiation-induced gastrointestinal syndrome

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Science China(Life Sciences) 2014年第12期57卷 1177-1182页

作者： YANG Chao dai wei min CHEN Hai Xu WU Ben Yan Gastrointestinal Department of Southern Building General Hospital of Chinese PLA Department of Thoracic Surgery General Hospital of Chinese PLA Institute of Gerontology and Geriatrics General Hospital of Chinese PLA

Nuclear accidents and terrorism present a serious threat for mass *** or intended radiation exposure leads to radiation-induced gastrointestinal(GI)***,currently there are no approved medical countermeasures for GI ***,developing novel treatments for GI syndrome is *** stem cells(MSCs)derived from bone marrow are a subset of multipotent adult somatic stem cells that have the ability to undergo self-renewal,proliferation and pluripotent *** have advantages over other stem cells;they can be easily isolated from patients or donors,readily expanded ex vivo,and they possess reparative and immunomodulatory ***,MSCs have been shown to be powerful tools in gene therapy and can be effectively transduced with vectors containing therapeutic ***,the therapeutic potential of MSCs has been brought into the spotlight for the clinical treatment of GI *** this review,we discuss the possible role of MSCs in radiation-induced GI syndrome.

关键词： mesenchymal stem cells radiation-induced gastrointestinal syndrome treatment

Effect of tillage and burial depth and density of seed on viability and seedling emergence of weedy rice

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Journal of Integrative Agriculture 2019年第8期18卷 1914-1923页

作者： ZHANG Zheng GAO Ping-lei dai wei-min SONG Xiao-ling HU Feng QIANG Sheng Weed Research Laboratory Nanjing Agricultural University Nanjing 210095 P.R.China College of Resources and Environmental Sciences Nanjing Agricultural University Nanjing 210095 P.R.China

Weedy rice(Oryza sativa f. spontanea) is one of the three worst paddy weeds in most rice growing areas. The unexpected heavy infestation is derived from a persistence of soil seed bank of weedy rice, which the shattered seeds chiefly feed back to. Information on soil seed bank dynamics is imperative to predict the infestation of weeds. In the present paper,the effect of rotary tillage on weedy rice seed bank structure was studied first, and a burial experiment of marked seeds was conducted to observe the overwintering survival, seed viability and seedling emergence of weedy rice. The results showed that the proportion of weedy rice seeds in deeper soil increased but seedling emergence decreased with increasing plowing depth. The viability of weedy rice seeds decreased as the burial duration time extended but more slowly in deeper soil layers. Additionally, there was no significant effect of burial density on seed viability. Moreover, the logistic model fitted well(R^2≥0.95, P≤0.01) with the depressive trends of seed viability with increasing burial time under all burial depths and densities which can provide us further information about seed survival. In field experiments, number of seedling emergence significantly decreased as seed burial depth increased, conversely, proportion of seedling emergence increased as seed burial density decreased. This study has important implications for determining strategies for weedy rice management by exhausting its seed bank through the alteration of tillage practices.

关键词： seed burial depth seed burial density germination wintering survival seed vertical distribution

Study on asymmetric synthesis of steroids——XIV. New synthesis of two optically active steroid CD synthons

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Chinese Journal of Chemistry 1986年第2期4卷 187-188页

作者： dai wei-min ZHOU wei-Shan*, Shanghai Institute of Organic Chemistry, Academia sinica, Shanghai Shanghai Institute of Organic Chemistry Academia sinica Shanghai

1a and 2a are important optically active steroid CD ring synthons used for total synthesisof 19-nor *** now wish to report herein a new synthesis of two optically active

关键词： New synthesis of two optically active steroid CD synthons Study on asymmetric synthesis of steroids XIV CD

Occurrence pattern and morphological polymorphism of Chinese weedy rice

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Journal of Integrative Agriculture 2023年第1期22卷 149-169页

作者： WANG Hao-quan dai wei-min ZHANG Zi-xu LI Meng-shuo MENG Ling-chao ZHANG Zheng LU Huan SONG Xiao-ling QIANG Sheng Weed Research Laboratory College of Life SciencesNanjing Agricultural UniversityNanjing 210095P.R.China

Rice,the main food crop in China,has been sporadically reported to suffer from weedy rice ***,the overall occurrence and distribution pattern of Chinese weedy rice remains unclear because a systematic survey has not been *** order to reveal the infestation of Chinese weedy rice,a field survey was conducted in 999 sampling sites all over the rice-growing regions in China from 2009 to 2016 using seven-scale visual scoring of the level of weed *** rice was found 39%occurrence incidence in a total of 387 *** sampling sites with 50%or higher overall weedy rice infestation index mainly radiated from Jiangsu,Heilongjiang,Ningxia and Guangdong to the whole East China,Northeast China,Northwest China and South China.A total of 45 morphological characters from 287 populations(collected simultaneously with the field survey)out of those occurred sites were observed and analyzed using multivariate analysis in common gardens with the same cultivation conditions in 2017 and *** correlation analysis showed that 45 morphological characters were significantly related to the latitude,mean temperature,minimum temperature,precipitation and mean diurnal range *** 287 weedy rice populations were divided into three morphological groups with climate-dependent geographical differentiation:strong tiller type only in Jiangsu,large leaf type in South China and Central China and large grain type mainly in North *** rice seriously infested rice fields and had a geography,climate and cultivated rice type-dependent morphological and biotype differentiation in *** is suggested to pay attention to the harmfulness of weedy rice and adopt comprehensive control strategies.

关键词： weedy rice infestation morphological characters diversity

Allele Types of Rc Gene of Weedy Rice from Jiangsu Province, China

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Rice science 2014年第5期21卷 252-261页

作者： LI Xiao-yan QIANG Sheng SONG Xiao-ling CAI Kun SUN Yi-na SHI Zhi-hua dai wei-min Weed Research Laboratory Nanjing Agricultural University

Weedy rice （Oryza sativa f. spontanea）, the predominant type of which has a red pericarp, seriously inhibits growth and yield of direct-seeded rice in Jiangsu Province, China. In this study, we randomly selected 10 weedy rice accessions from 10 plots in Jiangsu, and then sequenced the full lengths of their Rc genes （approximately 6.4 kb）. In addition, we collected 166 different full-length Rc genes in the Oryza genus from the literature and from GenBank. A collinearity sequence analysis showed that the 10 weedy rice accessions from Jiangsu all had the same wild-type allele of the Rc gene. Single nucleotide polymorphisms indicated that the nucleotide polymorphisms （π= 0.19） and the proportion of segregation sites （ew = 0.28） of the Rc genes in the 10 weedy rice accessions from Jiangsu were higher than those in 56 weedy rice accessions from USA （π = 0.09 and θw = 0.07）. Haplotype and phylogenetic analyses showed that the Rc genes of weedy rice accessions from Jiangsu were not revertants of the rc gene found in Asian cultivated rice （O. sativa） varieties with white pericarp. In addition, Rc gene sequences of the rice varieties Lvdao from Lianyungang, Jiangsu and Tangdao from Anhui were more similar to those of cultivated rice than to the weedy rice from Jiangsu. These findings support the continued quarantine of weedy rice and clarify the evolutionary mechanism of the red pericarp found in the weedy rice of Jiangsu.

关键词： weedy rice red pericarp Rc gene allele type origin

QTL Mapping of Chlorophyll Contents in Rice

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Agricultural Sciences in China 2007年第1期6卷 17-24页

作者： SHEN BO ZHUANG Jie-yun ZHANG Ke-qin dai wei-min LU Ye FU Li-qing DING Jia-ming ZHENG Kang-le National Center for Rice improvement and State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 P.R.China College of Biological Science Hangzhou Normal College Hangzhou 310012 P.R.China

The objectives of this study were to investigate the genetic factors controlling the chlorophyll content of rice leaf using QTL analysis. A linkage map consisting of 207 DNA markers was constructed by using 247 recombinant inbred lines （RILs） derived from an indica-indica rice cross of Zhenshan97B×Milyang 46. In 2002 and 2003, the contents of chlorophyll a and b of the parents and the 247 RILs were measured on the top first leaf, top second leaf, and top third leaf, respectively. The software QTLMapper 1.6 was used to detect quantitative trait loci （QTLs）, additive by environment （AE） interactions, and epistatic by environment （AAE） interactions. A total of eight QTLs in four intervals were detected to have significant additive effects on chlorophyll a and b contents at different leaf positions, with 1.96-9.77% of phenotypic variation explained by a single QTL, and two QTLs with significant AE interactions were detected. Epistasis analysis detected nine significant additive-by-additive interactions on chlorophyll a and b contents, and one pair of QTLs with significant AAE interactions was detected. On comparison with QTLs for yield traits detected in the same population, it was found in many cases that the QTLs for chlorophyll a and b contents and those for yield traits were located in the same chromosome intervals.

关键词： rice quantitative trait loci chlorophyll content yield traits QTL×environment interaction

城市化系统中的资源环境质量综合评价及政策选择——以安徽省为例

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中国软科学 2011年第11期 184-192页

作者：戴为民中国人民大学经济学院 100872 安徽财经大学经济学院 233041

城市化是人口、经济、社会、环境组成的复合系统,论文构建了资源环境质量综合评价指标体系,确定了指标警限的取值标准。基于安徽省1999-2008年的统计数据,对安徽省资源环境质量进行了综合评价。以此为依据,分析了制约安徽省资源环境质... 详细信息

城市化是人口、经济、社会、环境组成的复合系统,论文构建了资源环境质量综合评价指标体系,确定了指标警限的取值标准。基于安徽省1999-2008年的统计数据,对安徽省资源环境质量进行了综合评价。以此为依据,分析了制约安徽省资源环境质量的因素及其政策含义。

关键词：资源环境质量指标体系实证分析

Study on asymmetric syntheses of steroids ⅩⅢ. Synthesis of two new (±)-3-oxa-A-nor steroids 1a and 1b

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Chinese Journal of Chemistry 1986年第3期 230-238页

作者： ZHOU wei-Shan dai wei-min Shanghai Institute of Organic Chemistry, Academia Sinica, Shanghai

This paper deals with the synthesis of two new (±)-3-oxa-A-nor steroids 1a and 1 *** conjugated addition of 5'-methylfurandithane anion 8b to the CD-ring synthon 9 in HMPA-THFafforded 9, 10-seco-3-oxa-A-nor steroid 22. The intramolecular cyclization of 22 in acidic mediumgave 23, which underwent hydrolytic desulfurization at elevated temperature in acidic medium togive 1b. The desulfurization of 23 over Raney nickel gave 1a.

关键词： oxa-A-nor steroids 1a and 1b Study on asymmetric syntheses of steroids