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Nuclear Science and Techniques 2010年第6期21卷 347-351页

作者： jin Xiang Lue jie Ma Yuan Mao Peihong Xinjiang Univ Coll Phys & Technol Ctr Ion Beam Biotechnol Urumqi 830008 Peoples R China Xinjiang Univ Coll Resource & Environm Sci Urumqi 830046 Peoples R China Nanjing Univ Technol Coll Life Sci & Pharm Nanjing 210009 Peoples R China

Glycyrrhizic acid(GA) in Glycyrrhiza uralensis(***) is physiologically *** this study,the total DNA of wild *** was randomly transformed into Hansenula anomala by implantation of low-energy Ar^+ and N^+,to produce five recombinant yeast strains relating to biological synthesis of the GA or Glycyrrhetinic acid (GAs).After culturing in liquid medium for 96 h,the resultant GA,18α-GAs and 18β-Gas were determined by reversed-phase high performance liquid chromatography(RP-HPLC),and the corresponding concentrations were 114.49,0.56,and 0.81 mg·L^(-1).After one hundred primers were analyzed with random amplified polymorphic DNA (RAPD),the seven different DNA fragments were produced by the N7059 strain of recombined yeasts,and,the polymerase chain reaction(PCR) verified that one of them came from the genome of ***,indicating a successful transfer of genetic information by ion implantation.

关键词：异常汉逊酵母 DNA转化重组酵母离子注入甘草酸乌拉尔甘草随机扩增多态性DNA 反相高效液相色谱法

Nickel-Promoted Oxidative ipso-Annulation ofN-（p-Methoxyaryl）propiolamides with a-Carbonyl Alkyl Bromides

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Chinese Journal of Chemistry 2017年第3期35卷 299-302页

作者： Meng Li Ren-jie Song jin-Heng Li Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle Nanchang Hangkong University Nanchang Jiangxi 330063 China State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha Hunan 410082 China

A new route to producing 3-alkyl spiro[4,5]trienones via nickel-promoted radical oxidative ipso-annulation of N-(p-methoxyaryl)propiolamides with α-carbonyl alkyl bromides using TBHP oxidant is described. The method allows the formation of three new C—C bonds through a sequence of oxidative C—Br bond cleavage, ipso-cyclization and dearomatization, and represents a new alkyne 1,2-difunctionalization to access to the construction of spiro[4,5]trienones.

关键词： nickel radical ipso-annulation alkynes 3-alkyl spiro[4 5]trienones

Household and Community Assets and Farmers' Adaptation to Extreme Weather Event:the Case of Drought in China

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Journal of Integrative Agriculture 2014年第4期13卷 687-697页

作者： WANG Yang-jie HUANG Ji-kun WANG jin-xia Center for Chinese Agricultural Policy Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of Sciences University of Chinese Academy of Sciences

Under climate change, rising frequency and serious extreme weather events have challenged agricultural production. Designing appropriate adaptation measures to the extreme weather events require rigorous and empirical analysis. The overall goals of this study are to understand physical adaptation measures taken by farmers and the impacts of household and community assets on farmers＇ adaptation when they face drought. The analyses are based on a unique data set collected from a household survey in three provinces in China. The survey results show that though not common on annual basis, some farmers did use physical adaptation measures to fight drought. Regression analysis reveals that both household and community assets significantly affect farmers＇ adaptation behaviors. Improving households＇ social capital and wealth, communities＇ network and access to government＇s anti-drought service can facilitate farmers＇ adaptation to drought. Results indicate that community＇s irrigation infrastructure and physical adaptation taken by farmers can substitute each other. Further analysis shows that the households taking adaptation measures have higher crop yields than those without taking these measures. The paper concludes with several policy implications.

关键词： drought physical adaptation measure household community asset agriculture

Hlder Continuity for a Class of Strongly Degenerate Schrdinger Operator Formed by Vector Fields

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Acta Mathematicae Applicatae Sinica 2013年第2期29卷 281-288页

作者： Yong-yang jin jie-lin L Rong-fei LIN Department of Applied Mathematics Zhejiang University of Technology Zhejiang Vocational College of Commerce Department of Mathematics Taizhou University

In this paper we obtain the H61der continuity property of the solutions for a class of degenerate Schr6dinger equation generated by the vector fields：∑i,j=1^m Xj^＊（aij（x）Xiu）＋bXu=uu=0,where X = {X1,.-. ,Xm} is a family of C^∞ vector fields satisfying the H6rmander condition, and the lower order terms belong to an appropriate Morrey type space.

关键词： Hoe1der continuity degenerate SchrSdinger operator green function

Robust beam splitter with fast quantum state transfer through a topological interface

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Frontiers of physics 2023年第5期18卷 293-309页

作者： Jia-Ning Zhang jin-Xuan Han jin-Lei Wu jie Song Yong-Yuan Jiang Department of Optoelectronics Science Harbin Institute of TechnologyWeihai 264209China School of Physics Harbin Institute of TechnologyHarbin 150001China School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China Key Laboratory of Micro-Nano Optoelectronic Information System Ministry of Industry and Information TechnologyHarbin 150001China Key Laboratory of Micro-Optics and Photonic Technology of Heilongjiang Province Harbin Institute of TechnologyHarbin 150001China

The Su–Schrieffer–Heeger(SSH)model,commonly used for robust state transfers through topologically protected edge pumping,has been generalized and exploited to engineer diverse functional quantum ***,we propose to realize a fast topological beam splitter based on a generalized SSH model by accelerating the quantum state transfer(QST)process essentially limited by adiabatic *** scheme involves delicate orchestration of the instantaneous energy spectrum through exponential modulation of nearest neighbor coupling strengths and onsite energies,yielding a significantly accelerated beam splitting *** to properties of topological pumping and accelerated QST,the beam splitter exhibits strong robustness against parameter disorders and losses of *** addition,the model demonstrates good scalability and can be extended to two-dimensional crossed-chain structures to realize a topological router with variable numbers of output *** work provides practical prospects for fast and robust topological QST in feasible quantum devices in large-scale quantum information processing.

关键词： quantum state transfer beam splitter topological router

Multi-channel microfluidic chip-mass spectrometry platform for cell analysis

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Chinese Chemical Letters 2017年第8期28卷 1625-1630页

作者： Mingsha jie Sifeng Mao Haifang Li jin-Ming Lin State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Department of Chemistry Beijing Key Laboratory of Microanalytical Methods and Instrumentation The Key Laboratory of Bioorganic Phosphorus Chemistry& Chemical Biology Tsinghua University

In this review, we highlight the latest development of multi-channel microfluidic chip-mass spectrometry（chip-MS） in cell analysis and metabolite detection. Following a brief introduction about history and development of multi-channel microchip and MS combination, we will elaborate the key issues of constructing chip-MS platform interface. Then exciting progresses made in this field should be reviewed with well exemplified works, including chip-MS technology for cell introduction, pretreatment of cell secretions and cell metabolite analysis. We will also describe the development of integrated total analysis systems proposed by our group. We hope this brief review will inspire interested readers and provide knowledge about chip-MS platform in the bioanalysis field, particularly in cell analysis and metabolite identifying applications.

关键词： Chip-MS platform Interface technology Cell analysis Metabolite identification

A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication

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Bioactive Materials 2022年第12期7卷 138-150页

作者： Zeyu Zhang Chenming Wu Chengkai dai Qingqing Shi Guoxin Fang Dongfang Xie Xiangjie Zhao Yong-jin Liu Charlie CLWang Xiu-jie Wang Institute of Genetics and Developmental Biology Innovation Academy of Seed DesignChinese Academy of SciencesBeijing100101China Beijing National Research Center for Information Science and Technology Department of Computer Science and TechnologyTsinghua UniversityBeijing100084China Department of Mechanical Aerospace and Civil EngineeringThe University of ManchesterManchesterM204BXUK Faculty of Industrial Design Engineering Delft University of TechnologyDelft2628the Netherlands University of Chinese Academy of Sciences Beijing100049China

Despite the recent advances in artificial tissue and organ engineering,how to generate large size viable and functional complex organs still remains as a grand challenge for regenerative ***-dimensional bioprinting has demonstrated its advantages as one of the major methods in fabricating simple tissues,yet it still faces difficulties to generate vasculatures and preserve cell functions in complex organ ***,we overcome the limitations of conventional bioprinting systems by converting a six degree-of-freedom robotic arm into a bioprinter,therefore enables cell printing on 3D complex-shaped vascular scaffolds from all *** also developed an oil bath-based cell printing method to better preserve cell natural functions after *** with a self-designed bioreactor and a repeated print-and-culture strategy,our bioprinting system is capable to generate vascularized,contractible,and long-term survived cardiac *** bioprinting strategy mimics the in vivo organ development process and presents a promising solution for in vitro fabrication of complex organs.

关键词： Six degree-of-freedom robot 3D bioprinting Artificial organ engineering Print-and-culture Cardiac tissue fabrication

Improving tribological and corrosion resistance of Ti6Al4V alloy by hybrid microarc oxidation/enameling treatments

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Rare Metals 2018年第1期37卷 26-34页

作者： jie jin Xiao-Han Li Ji-Wen Wu Bai-Yang Lou College of Material Science and Engineering Zhejiang University of Technology

A promising duplex coating was prepared by microarc oxidation（MAO） and enameling processes onto polished Ti6A14V alloy. The TiO2 ceramic coating deposited by MAO was characterized and then combined with an enameling treatment in order to improve the tribological and corrosion resistance of Ti6A14V alloy. The morphology, phase composition, and hardness of MAO and MAO/enameling-coated Ti6A14V alloy were evaluated by scanning electron microscopy（SEM）, X-ray diffraction（XRD）, and Vickers microhardness tester, *** tribological performance was investigated using a ballon-disk tribometer. The corrosion resistance was studied using immersion tests and potentiodynamic *** tests show that the enamel coating on the MAOcoated surface causes a reduction in the friction *** tests demonstrate that the duplex coating is more effective in improving the corrosion resistance of Ti6A14V than the MAO coating especially at high temperature（80 ℃）. Potentiodynamic polarization curves reveal that the corrosion potential of the duplex coating increases by about 250 mV and the corrosion current density is slightly lower than that of the MAO coating. The duplex coating is superior to the stand-alone MAO coating in improving the tribological and corrosion behavior of Ti6A14V.

关键词： Ti6Al4V Microarc oxidation Enameling Tribological Corrosion

A novel DPSS filter optimization scheme to reduce the intrinsic interference of FBMC-QAM systems

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High Technology Letters 2024年第1期30卷 68-74页

作者：董琪阳 MA Tianming jin jie WU Zhong School of Electrical and Electronic Engineering Shanghai University of Engineering ScienceShanghai 201620P.R.China

In order to reduce the intrinsic interference of the filter bank multicarrier-quadrature amplitude modulation(FBMC-QAM)system,a novel filter optimization scheme based on discrete prolate spheroidal sequences(DPSS)is ***,a prototype filter function based on DPSS is designed,since the eigenvalue can be used as an indicator of the energy concentration of DPSS,so a threshold is set,and the sequence with the most concentrated energy is selected under the threshold,that is,the sequence with the eigenvalue higher than the threshold,and the prototype filter function is rewritten as a weighted sum function of multiple *** the energy constraints of the filter,the relationship between the eigenvectors and the intrinsic interference function is established,and the function problem is transformed into an optimization problem for the weighted *** the interior point method,the most suitable weight is found to obtain the minimum intrinsic interference *** analysis and simulation results show that compared with the prototype filters such as Type1 and CaseC,the DPSS filter applying the proposed optimization algorithm can effectively suppress the intrinsic interference of the system and obtain a better bit error rate(BER)performance.

关键词： filter bank multicarrier-quadrature amplitude modulation(FBMC-QAM) intrinsic interference discrete prolate spheroidal sequences(DPSS) interior point method

Preliminary screening and identification of stem cell-like sphere clones in a gallbladder cancer cell line GBC-SD

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第4期12卷 256-263页

作者： Bao-bing YIN Shuang-jie WU Hua-jie ZONG Bao-jin MA Duan CAI Department of General Surgery Huashan Hospital Fudan University Shanghai 200040 China

This paper aims to screen and identify sphere clone cells with characteristics similar to cancer stem cells in human gallbladder cancer cell line ***-SD cells were cultured in a serum-free culture medium with different concentrations of the chemotherapeutic drug cisplatin for generating sphere *** mRNA expressions of stem cell-related genes CD133,OCT-4,Nanog,and drug resistance genes ABCG2 and MDR-1 in sphere clones were detected by quantitative real-time polymerase chain reaction (PCR).Stem cell markers were also analyzed by flow cytometry and immunofluorescent *** amounts of sphere clones were injected into nude mice to test their abilities to form *** clones were formed in serum-free culture medium containing cisplatin (30 μmol/L).Flow cytometry results demonstrated that the sphere clones expressed high levels of stem cell markers CD133+ (97.6%) and CD44+ (77.9%) and low levels of CD24+ (2.3%).These clones also overexpressed the drug resistance genes ABCG2 and *** real-time PCR showed that sphere clones expressed stem cell genes Nanog and OCT-4 284 and 266 times,respectively,more than those in the original GBC-SD *** staining showed that sphere clones overexpressed OCT-4,Nanog,and SOX-2,and low expressed MUC1 and *** formation experiments showed that 1×103 sphere clone cells could induce much larger tumors in nude mice than 1×105 GBC-SD *** conclusion,sphere clones of gallbladder cancer with stem cell-like characteristics can be obtained using suspension cultures of GBC-SD cells in serum-free culture medium containing cisplatin.

关键词： Gallbladder cancer Stem cell gene Sphere clone Suspension culture