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HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana

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Chemical Research in Chinese Universities 2015年第1期31卷 38-43页

作者： WANG Hui WANG Jiayuan WU Xiaopeng SHEN Haiyan LUO Ying dai haofu MEI Wenli Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine Key Laboratory of Tropical Crop Biotechnology Ministry of Agriculture Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 P. R. China

Dragon＇s blood is a traditional medicine and used in many countries with different cultures because of its various therapeutic properties. The main bioactive constituents of dragon＇s blood are flavonoids, which exhibit various pharmaceutical activities, such as haemostatic, analgesic, anticoagulant, and antimicrobial activities and so on, and have attracted the attention of researchers on the development of new drugs. However, the formation process of dragon＇s blood in nature is very slow, which cannot meet the demand of pharmaceutical uses. Dracaena cambodiana （D. cambodiana） is one of the resource plants of dragon＇s blood na, 6-benzylaminopurine was added in the medium, then red During the course of tissue culture of D. cambodiasecretion was discovered in the cultured medium. Twelve compounds（1--12）, including 11 flavonoids, were determined via the analysis of constituents of the cultured medium by high performance liquid chromatography-electrospray ionization-mass spectrometry（HPLC-ESI-MS） and the results were compared with that of the standards isolated from dragon＇s blood. Among compounds 1-12, 4,4＇- dihydroxy-2-methoxydihydrochalcone（5）, 4,4＇-dihydroxy-2,6-dimethoxydihydrochalcone（6） and （2S）-7,3＇-dihydroxy- 4＇-methoxyflavane（9） were major compounds of the red secretion, with contents of 15.70, 7.10 and 57.23 mg/L, respectively. Therefore, it is promising that flavonoids from dragon＇s blood can be obtained from the tissue culture of its resource plants for the purpose of drug development.

关键词： Flavonoid Dragon's blood High performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS) Dracaena cambodiana Tissue culture

Qualitative and Quantitative Analysis of Flidersiachromones in Three Agarwood Samples by HPLC-MS/MS

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Chemical Research in Chinese Universities 2018年第3期34卷 389-396页

作者： YANG Jinling DONG Wenhua CHEN Huiqin KONG Fandong WANG Jun MEI Wenli dai haofu Hainan Engineering Research Center of Agarwood Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 P. R. China

HPLC/ESI/MS2 was used in the qualitative and quantitative analysis of flidersiachromones（FTPECs） in three agarwood samples ofAquilaria sinensis, including an artificial holing agarwood, a wild agarwood, and a high quality agarwood named ＂Qi Nan＂. From these three agarwood samples, totally forty-six FTPECs were identified, and twenty-five of which were tentatively identified by the analysis of MS fragmentation ions, the others were indentiffed via comparing the retention time and MS fragmentation ions of them with those of the reference compounds. Besides, quantitative determination of five characteristic FTPECs（F14, F16 and F18-F20） in the three agarwoods was carried out, and it was found that the absolute content of each compound of these five FTPECs showed positive correlation with the quality of agarwood samples. Due to the wide distribution of four of these FTPECs（F16 and F18-F20） in the chemical constituents of agarwood, it is suggested that the total absolute content of these four FTPECs may be set as one of basis for agarwood quality evaluation.

关键词： Agarwood Aquilaria sinensis HPLC/DAD/ESI/MS/MS Flidersiachromone Quantitative determination

Isolation and identification of multidrug-resistant Staphylococcus haemolyticus from a laboratory-breeding mouse

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Asian Pacific Journal of Tropical Medicine 2011年第6期4卷 421-425页

作者： Fengying Huang Qiuping Meng Guanghong Tan Yonghao Huang Hua Wang Wenli Mei haofu dai Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical College Agriculture School Hainan University Biological Technology Institute Chinese Academy of Tropical Agricultural Sciences

Objective:To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away from a ***:Phenotype of the isolate was investigated by conventional microbiological methods,including Gram-staining,colony morphology,tests for haemolysis, catalase,coagulase,and antimicrobial susceptibility *** meek and 16S rRNA genes were amplified by the polymerase chain reaction(PCR) and *** base sequence of the PCR product was compared with known 16S rRNA gene sequences in the CenBank database by phylogenetic analysis and multiple sequence ***:The isolate in this study was a gram positive,coagulase negative,and catalase positive *** isolate was resistant to oxacillin,methicillin,penicillin,ampicillin,cefazolin,cipr of loxacin erythromycin,et *** results indicated that the isolate was meek gene positive and its 16S rRNA was 1465 *** analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus,and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the ***: 16S rRNA gene sequencing is a suitable technique for non-specialist *** animals are possible sources of lethal pathogens,and researchers must adapt protective measures when they manipulate animals.

关键词： 16S rRNA Gene sequences analysis Staphylococcus haemolyticus Multidrug resistant

New indole derivatives from endophytic fungus Colletotruchum sp. HK-08 originated from leaves of Nerium indicum

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Chinese Herbal Medicines 2024年第2期16卷 235-238页

作者： Huiqin Chen Hao Zheng Caihong Cai Hao Wang Cuijuan Gai Zhiqiong Tan haofu dai Wenli Mei Hainan Key Laboratory of Research and Development of Natural Product from Li Folk Medicine Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101China Hainan Institute for Tropical Agricultural Resources Haikou 571101China School of Life and Pharmaceutical Sciences Hainan UniversityHaikou 570228China

Objective: To study secondary metabolites from endophytic fungus Colletotruchum sp. HK-08 originated from the leaves of Nerium ***: The compounds were isolated by various column chromatographic techniques, and their structures were elucidated by spectroscopic techniques [high resolution electrospray ionization mass spectroscopy(HRESIMS), one-dimensional(1D) and two-dimensional(2D) nuclear magnetic resonance spectroscopy(NMR)], as well as comparison with literature data. The Ellman method was used to determine the acetylcholinesterase(ACh E) inhibitory ***: Four indole derivatives were identified from Colletotruchum sp. HK-08, including 6’-hydroxymonaspiloindole(1), 2-(2-oxoindolin-3-yl)ethyl 2-(4-hydroxyphenyl) acetate(2), 2-(2-oxoindolin-3-yl)ethyl 2-(2-hydroxyphenyl)acetate(3), and monaspiloindole(4). Compound 4 presented weak ACh E inhibitory activity with IC50value of(69.30 ± 6.27) μmol/L [tacrine as the positive control, with IC50value of(0.61 ± 0.07) μmol/L].Conclusion: Compounds 1–3 were new compounds, and compound 4 had weak ACh E inhibitory activity.

关键词： Colletotruchum sp. endophytic fungus indole derivatives Nerium indicum Mill. secondary metabolites

Catechol-derived Inhibitors against Quorum Sensing from Retiboletus kauffmanii

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Medicinal Plant 2021年第1期12卷 68-70页

作者： Liman ZHOU Lin WANG Fandong KONG Qingyun MA Qingyi XIE haofu dai Youxing ZHAO Haikou Key Laboratory for Research and Utilization of Tropical Natural Products Institute of Tropical Bioscience and BiotechnologyCATASHaikou 571101China Hainan Academy of Tropical Agricultural Resource CATASHaikou 571101China

[Objectives]This study aimed to screen anti-quorum sensing(QS)activity from the constituent of Retiboletus kauffmanii fruit body.[Methods]Chromatographic technology was used to isolate compounds,while well diffusion assay was applied to screen anti-quorum sensing activity.[Results]12 phenolic compounds were purified from extracts,three catechol-derived compounds at sub-inhibitory concentrations were found to inhibit the production of violacein in Chromobacterium violaceum for the first time.[Conclusions]Three catechol analogs isolated from *** fruit body extract were indicated as quorum sensing inhibitors against ***.

关键词： Chromobacterium violaceum Quorum sensing inhibitor Retiboletus kauffmanii Virulence factors

Chemical constituents from agarwood of Aquilaria filaria

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Journal of Chinese Pharmaceutical Sciences 2021年第6期30卷 505-517页

作者： Chengneng Mi Wenli Mei Jingzhe Yuan Wenxing Long haofu dai Hainan Engineering Research Center of Agarwood Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101China Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine Haikou 571101China Institute of Tropical Agriculture and Forestry Hainan UniversityHaikou 570228China

Investigations on the chemical constituents from the ethanol extract of the agarwood originating from Aquilaria filaria led to the isolation of 26 compounds by column chromatography,including silica gel,Sephadex LH-20,and semi-preparative high-performance liquid *** structures were determined to beβ-sitostenone(1),24α-ethyl-cholestan-3α-ol(2),ergosta-4,6,8(14),22-tetraene-3-one(3),gypsogenin(4),hederagenin(5),17αH-trisnorhopanone(6),lupeol(7),friedelin(8),(-)-episyringaresinol(9),(-)-syringaresinol(10),5-hydroxy-7,4′-dimethoxyflavone(11),5,4’-dihydroxy-7,3′-dimethoxyflavone(12),2′-hydroxy-4,3′,6′,4′′-tetramethoxy-p-terphenyl(13),scopularide A(14),4-(4-hydroxyphenyl)butan-2-one(15),zingerone(16),coniferaldehyde(17),scopoletin(18),acetovanillon(19),isovanillin(20),vanillin(21),p-hydroxybenzaldehyde(22),p-hydroxybenzoic acid(23),protocatechuic acid(24),vanillic acid(25)and 4,5-dihydroxy-3-methoxybenzoic acid(26)by using spectroscopic *** compounds were isolated from agarwood of *** for the first time,and this was also the first report about the isolation of compounds 2,4,6,7,9,10,13-15,17-20,22,24 and 26 from agarwood and Aquilaria plants.

关键词： Agarwood of Aquilaria filaria Chemical constituents Triterpene Steroid

草豆蔻种子化学成分及其NF-κB的激活抑制作用与抗肿瘤活性

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中国中药杂志 2010年第13期35卷 1710-1714页

作者：唐俊李宁戴好富王开金安徽大学生命科学学院安徽省中药研究与开发重点实验室安徽合肥230039 中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室海南海口571101

目的:研究草豆蔻Aplinia katsumadai化学成分及其NF-κB的激活抑制作用与抗肿瘤活性。方法:采用硅胶和Sephadex LH-20等色谱手段进行化合物的分离纯化,通过波谱方法结合理化性质鉴定其化学结构,采用High-Content Screening(image-based... 详细信息

目的:研究草豆蔻Aplinia katsumadai化学成分及其NF-κB的激活抑制作用与抗肿瘤活性。方法:采用硅胶和Sephadex LH-20等色谱手段进行化合物的分离纯化,通过波谱方法结合理化性质鉴定其化学结构,采用High-Content Screening(image-based)免疫荧光法对所得到化合物进行TNF诱导人肺癌细胞A549的NF-κB的激活抑制实验,采用MTT法进行体外抗肿瘤活性检测。结果:从草豆蔻乙酸乙酯部位分离得到12个化合物,分别鉴定为(3S,5S)-trans-3,5-dihydroxy-1,7-diphenyl-hept-1-ene(1),(3R,5S)-trans-3,5-dihydroxy-1,7-diphenyl-hept-1-ene(2),5-hydroxy-1,7-diphenyl-hepta-6-en-3-one(3),豆蔻明(4),山姜素(5),乔松素(6),球松素(7),柚皮素(8),(+)-儿茶素(9),白杨素(10),芦丁(11),2,4-二羟基-6-苯乙基-苯甲酸甲酯(12);化合物1～4具有NF-κB激活抑制作用,IC50值分别为14.8,16.5,23.2,7.5μmol.L-1;化合物4对人白血病K562和肝癌SMMC-7721的IC50分别为3.2,3.5 mg.L-1;化合物6对肝癌SMMC-7721显示中等活性,IC50为18.3 mg.L-1。结论:化合物7～12均首次在该种中分离得到,12为山姜素属中首次发现,查耳酮类化合物4具有较强的NF-κB激活抑制作用和细胞毒活性,二苯基庚烷类成分(1～3)具有一定NF-κB激活抑制作用。

关键词：草豆蔻化学成分 NF-κB激活抑制抗肿瘤活

Chemical constituents and their antitumor activities from Ganoderma tropicum(Jungh.) Bres.

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Chemical constituents and their antitumor activities from Ga...

中国化学会第9届天然有机化学学术会议

作者： HU Lili,MA Qingyun,HUANG Shengzhuo,dai haofu,ZHAO Youxing~* Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China

The family Ganodermataceae comprising more than 200 fungi species is mainly distributed in the tropical and subtropical areas of Asia,Australia,Africa and America,among which more than 100 species grow in China and 78 wild species were found in Hainan *** chemical investigation on the fruiting bodies of Ganoderma tropicum(Jungh.) Bres.,wild "Lingzhi"genus in Hainan Province,led to the isolation of thirty two compounds (1-32) including four new compounds by column chromatography on silica gel and sephadex *** structures mainly including triterpenes,steroids and xanthones were elucidated by NMR analysis as well as comparison with those reported in the literatures,and were reported for the first time from Ganoderma *** was found for the first time from Ganoderma species,which maybe one of the important natural pigment contributed to the yellow color of the fruiting bodies of *** bioassay of cytotoxic activities of nine compounds was evaluated against five human tumor cell lines HL-60,SMMC-7721, A-549,MCF-7,and ***(1) displayed obvious cytotoxic activities on HL-60, SMMC-7721,A-549,MCF-7 and SW-480 with IC50 values of 7.49,9.04,4.44,21.61 and 14.02μmol.L,respectively.22E-7α-methoxy -5α,6α-epoxyergosta-8(14),22-dien-3β-ol(10) also exhibited definite cytotoxic activities on HL-60,MCF-7,and SW-480.

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海南龙血树抗肿瘤新用途研究

中药材 2005年第10期28卷 12-14页

作者：梅文莉戴好富吴娇庄令洪葵中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室海口571101

目的:筛选海南龙血树的抗肿瘤活性部位。方法:分别用溶剂萃取法和大孔吸附树脂柱层析法对海南龙血树的95%乙醇提取物进行分段处理,利用MTT法测定各提取部位体外抗肿瘤活性。结果:氯仿提取物、乙酸乙酯提取物和正丁醇提取物对体外肿瘤细... 详细信息

目的:筛选海南龙血树的抗肿瘤活性部位。方法:分别用溶剂萃取法和大孔吸附树脂柱层析法对海南龙血树的95%乙醇提取物进行分段处理,利用MTT法测定各提取部位体外抗肿瘤活性。结果:氯仿提取物、乙酸乙酯提取物和正丁醇提取物对体外肿瘤细胞表现出较强的抑制活性,甲醇洗脱物显示了弱活性,水提取、水洗脱物和50%甲醇-水洗脱物则没有活性。在各提取物中氯仿提取物活性最强。结论:氯仿提取部位是海南龙血树主要的体外抗肿瘤活性部位。

关键词：海南龙血树抗肿瘤活性 MTT