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LncRNA4667 is Dispensable for Spermatogenesis and Fertility in Mice

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Reproductive and Developmental Medicine 2019年第1期3卷 18-23页

作者： Yu-bing dai Yu Lin Ning Song Fei Sun Department of Human Anatomy Histology and EmbryologySchool of Basic MedicineQingdao UniversityQingdao 266071ShandongChina Institute of Embryo-Fetal Original Adult Disease International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200030China Shanghai Key Laboratory for Reproductive Medicine School of MedicineShanghai Jiao Tong UniversityShanghai 200030China Institute of Reproductive Medicine School of MedicineNantong UniversityNantong 226001China

Objective:Spermatogenesis is a complex process which is of vital importance for sexual *** many studies of spermatogenesis,the mRNAs,protein-coding genes,as well as small noncoding RNAs(ncRNAs)have been well ***,there remain numerous questions despite previously characterized molecular *** ncRNAs(lncRNAs)are a relatively new addition to our knowledge of *** studies have examined the function of lncRNAs in ***,we selected a testis-specific lncRNA,lncRNA4667,to analyze its potential role in spermatogenesis and male ***:In situ hybridization and quantitative reverse transcription polymerase chain reaction analyses were used to confirm testis-specific expression of ***4667 knockout mice were generated using CRISPR/Cas9 ***,sperm counts,sperm motility,body parameters,and fertility were compared between wild-type and knockout mice(n=8/group).Results:Expression analysis showed that lncRNA4667 was testis specific and localized to round spermatids in seminiferous tubules of adult mouse *** homozygous for a null mutation of lncRNA4667 displayed normal spermatogenesis and fertility compared with wild-type ***:These data indicate that lncRNA4667 is dispensable for spermatogenesis and fertility in mice,and the localization of lncRNA4667 makes it a useful marker for the identification of round spermatids in mice.

关键词： Long Noncoding RNA Male Infertility Spermatogenesis

Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme‑Induced Proximity Labeling

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Nano-Micro Letters 2024年第6期16卷 14-37页

作者： Junji Ren Zibin Zhang Shuo Geng Yuxi Cheng Huize Han Zhipu Fan Wenbing dai Hua Zhang Xueqing Wang Qiang Zhang bing He Department of Pharmaceutics School of Pharmaceutical Sciences Peking University38 Xueyuan RdHaidian DistrictBeijing 100191People’s Republic of China

Achieving increasingly finely targeted drug delivery to organs,tissues,cells,and even to intracellular biomacromolecules is one of the core goals of *** the delivery destination is refined to cellular and subcellular targets,it is essential to explore the delivery of nanomedicines at the molecular ***,due to the lack of technical methods,the molecular mechanism of the intracellular delivery of nanomedicines remains unclear to ***,we develop an enzyme-induced proximity labeling technology in nanoparticles(nano-EPL)for the real-time monitoring of proteins that interact with intracellular ***(lactic-co-glycolic acid)nanoparticles coupled with horseradish peroxidase(HRP)were fabricated as a model(HRP(+)-PNPs)to evaluate the molecular mechanism of nano delivery in *** adding the labeling probe biotin-phenol and the catalytic substrate H_(2)O_(2)at different time points in cellular delivery,nano-EPL technology was validated for the real-time in situ labeling of proteins interacting with ***-EPL achieves the dynamic molecular profiling of 740 proteins to map the intracellular delivery of HRP(+)-PNPs in macrophages over *** on dynamic clustering analysis of these proteins,we further discovered that different organelles,including endosomes,lysosomes,the endoplasmic reticulum,and the Golgi apparatus,are involved in delivery with distinct participation *** importantly,the engagement of these organelles differentially affects the drug delivery efficiency,reflecting the spatial–temporal heterogeneity of nano delivery in *** summary,these findings highlight a significant methodological advance toward understanding the molecular mechanisms involved in the intracellular delivery of nanomedicines.

关键词： Enzyme-induced proximity labeling Intracellular delivery Nano-protein interaction Dynamic molecule profiling Macrophages

Implementing a “free” tuberculosis (TB) care policy under the integrated model in Jiangsu, China: practices and costs in the real world

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Infectious Diseases of Poverty 2016年第1期5卷 1-8页

作者： Xinxin Jia Jiaying Chen Siyuan Zhang bing dai Qian Long Shenglan Tang Center for Health Policy Studies Nanjing Medical UniversityHanzhong Road 140210029 NanjingChina The Second Affiliated Hospital of Soochow University SuzhouJiangsu ProvinceChina Zhenjiang Center for Disease Control and Prevention ZhenjiangJiangsu ProvinceChina Duke Global Health Institute Duke UniversityDurhamNCUSA Global Health Research Center Duke Kunshan UniversityKunshanChina

Background:In the 1990s,China introduced a“free”tuberculosis(TB)care policy under the national TB control ***,as a part of a new TB diagnosis and treatment model,it has been recommended that the integrated model scale *** paper examines whether or not TB designated hospitals in the selected project sites have provided TB care according to the national and local guidelines,and analyzes the actual practices and expenditures involved in completing TB *** also explores the reasons why“free”TB care in China cannot be effectively implemented under the integrated ***:This study was conducted in three counties of Zhenjiang city,Jiangsu *** methods were used,which comprised reviewing the national and local TB control guidelines,conducting TB patient surveys,collecting TB inpatient and outpatient hospital records,and conducting qualitative interviews with *** statistics were used for quantitative data analysis across counties and in order to compare patients who received only outpatient care and those who received both outpatient and inpatient *** chi-square test and analysis of variance were performed where *** data were analyzed using the framework ***:Although the national TB care guidelines recommend outpatient care as a basis for TB treatment in China,we found high hospital admission rates for TB patients ranging from 39%in Yangzhong county to 83%in Dantu *** all outpatient TB patients paid for lab tests and over 80%paid for liver protection drugs and around 70%paid for image *** three components accounted for three-quarters of the total outpatient *** patients who received only outpatient care,the total expenditure upon completion of TB treatment was on average 1,135 Chinese *** patients who received outpatient and inpatient care,the total expenditure upon completion of TB treatment was 11,117 Chinese ***:The“free”

关键词： Tuberculosis Anti-TB treatment practice guidelines Free Expenditure Jiangsu province China

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印度对1964年中国原子弹爆炸的反应

南亚研究 2012年第3期 75-86页

作者：代兵南京政治学院

1964～1968年间,在中国核试验成功后,印度国内围绕核政策选择问题展开讨论。1964～1966年,讨论的焦点是印度是否要发展核武器,结论是印度保有发展和平目的核爆炸装置的权利。1964～1967年,印度政府又试探大国向本国提供核保护伞的可能性... 详细信息

1964～1968年间,在中国核试验成功后,印度国内围绕核政策选择问题展开讨论。1964～1966年,讨论的焦点是印度是否要发展核武器,结论是印度保有发展和平目的核爆炸装置的权利。1964～1967年,印度政府又试探大国向本国提供核保护伞的可能性,但也没有如愿。1967～1968年,印度核政策选择争论的核心转变为是否签署《核不扩散条约》。几经谈判,印度对此条约予以拒绝。

关键词：印度中国核政策选择

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误读三题

山花 2008年第7期 121-126页

作者：戴冰

不存在的分界——读博尔赫斯《门槛旁边的人》据博尔赫斯自己说,小说的起因是阿根廷作家卡萨雷斯从伦敦给他带来一柄印度的三棱形匕首,从而引发了他的想象。故事的情节非常简单:一个伊斯兰城市发生了骚乱,中央政府派出一个叫大卫·亚历... 详细信息

不存在的分界——读博尔赫斯《门槛旁边的人》据博尔赫斯自己说,小说的起因是阿根廷作家卡萨雷斯从伦敦给他带来一柄印度的三棱形匕首,从而引发了他的想象。故事的情节非常简单:一个伊斯兰城市发生了骚乱,中央政府派出一个叫大卫·亚历山大·格兰凯恩的铁腕人物前去恢复秩序。几年之后,秩序恢复了,格兰凯恩却在此时神秘失踪。一名探员,亦即是小说的

关键词：博尔赫斯万里长城卡夫卡《草莽集》终极真理屠格涅夫

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头发的故事

山花 2005年第6期 63-70页

作者：戴冰

中学以前,马天的外号一共有三个:“泡粑”、“踏踢猪”和“闷罐”。三个外号分别跟他的相貌、身材以及由此导致的一声不吭的性格有关。许多邻居都在背后感慨,说小马天怎么就像个垃圾桶似的,把他父母浑身上下的缺点收罗得一干二净。但让... 详细信息

中学以前,马天的外号一共有三个:“泡粑”、“踏踢猪”和“闷罐”。三个外号分别跟他的相貌、身材以及由此导致的一声不吭的性格有关。许多邻居都在背后感慨,说小马天怎么就像个垃圾桶似的,把他父母浑身上下的缺点收罗得一干二净。但让马天的父母聊以自慰的是,他们的儿子长着一头浓密得几乎让人难以置信的头发,而且随着年龄的增长,这一特点变得尤为突出,所以到了初中,马天的外号就变成了“头发”。这个外号乍一听起来有些怪哩怪气的,但所有的人在仔细考虑过之后,都觉得没有比这个外号更贴切的了。马天本人也很乐意别人这样叫他,对

关键词：头发的故事年轻人

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海河交汇区水泥搅拌桩加固技术初探

地基处理 2024年第2期6卷 162-171页

作者：杨文军吴毅航门小雄郭保和刘辉戴北冰刘建坤中铁隧道局集团有限公司广东广州511458 中山大学土木工程学院广东广州510275 珠海市轨道交通有限公司广东珠海519100 南方海洋科学与工程广东省实验室(珠海) 广东珠海519082

本文依托珠海隧道工程,针对超深欠固结淤泥地层,进行一系列不同配比、施工工艺、水灰比和QC-固化剂比例条件下的水泥搅拌桩加固试桩试验,系统研究了多种参数影响下辅道、明挖基坑和海域浅覆土加固试桩的成桩效果,发现掺入固化剂可获得... 详细信息

本文依托珠海隧道工程,针对超深欠固结淤泥地层,进行一系列不同配比、施工工艺、水灰比和QC-固化剂比例条件下的水泥搅拌桩加固试桩试验,系统研究了多种参数影响下辅道、明挖基坑和海域浅覆土加固试桩的成桩效果,发现掺入固化剂可获得更好的成桩效果和更优良的抗压性能。经加固处理的芯样其强度大部分在1~3 MPa,部分芯样强度可高达5 MPa,且桩身更为连续和完整。在其他参数相同的条件下,QC-固化剂占比高的芯样具有更好的完整性和强度,而水灰比对成桩效果及抗压强度的影响不大,四搅和两搅的施工工艺在单点土体切削次数达到20次或以上时,其对成桩效果和强度的影响无明显区别。最后对加固后的软土开展了微观电镜扫描试验,对比分析了经QC-固化剂与纯水泥固化剂加固后生成晶体对土体孔隙的填充现象,发现QC-固化剂对土体孔隙具有更好的填充效果。本水泥搅拌桩加固试桩试验在珠海海河交汇区软弱地层取得了较好的加固效果,可为沿海地区类似地质条件下的地基处理提供参考。

关键词：试桩加固淤泥层固化剂水泥搅拌桩软土隧道

“追名弃利”：权力动机与社会存在对亲社会行为的影响

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心理学报 2020年第1期52卷 55-65页

作者：王建峰戴冰成都医学院心理学院四川应用心理学研究中心

目前关于权力动机的研究主要关注权力动机的阴暗面,但是对权力动机的积极面尚不清楚。本研究从公平与合作行为的角度出发,分别采用最后通牒博弈和公共物品博弈任务,探讨不同权力动机水平个体在内隐(眼睛线索)或外显(他人在场)社会存在... 详细信息

目前关于权力动机的研究主要关注权力动机的阴暗面,但是对权力动机的积极面尚不清楚。本研究从公平与合作行为的角度出发,分别采用最后通牒博弈和公共物品博弈任务,探讨不同权力动机水平个体在内隐(眼睛线索)或外显(他人在场)社会存在下的亲社会行为是否不同。结果发现,在眼睛线索或他人在场条件下,相对于低权力动机者,高权力动机者表现出更高的公平与合作水平。然而当没有社会存在线索时,高低权力动机者的亲社会行为没有显著差异。结果提示高权力动机者出于名誉和地位的策略考虑,也会表现出积极的亲社会行为。

关键词：权力动机社会存在名誉公平合作亲社会行为

Rspo2 promotes embryonic stem cells to differentiate towards smooth muscle cells through LGR5/β-catenin pathway

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中国病理生理杂志 2016年第8期32卷 1531-1532页

作者： WU Yu-tao YANG Mei WANG Jing YANG Feng dai bing WANG Wei-na CHEN Ting ZHOU Yi-jiang ZHANG Li Department of Cardiology First Affiliated HospitalSchool of MedicineZhejiang University

AIM: R-spondin 2( Rspo2),one member of R-spondin family which contains four secreted proteins,plays an important role in skeletal muscle development. However,the impact of Rspo2 on vascular smooth muscle cell( SMC) differentiation is little *** study aims at revealing the role and mechanism of Rspo2 on SMC differentiation from embryonic stem cells( ESCs). METHODS:A well-established model for studying SMC differentiation from ESCs were used,in which mouse embryonic stem cells( ES-D3) were seeded on collagen IV-coated asks and cultured in differentiation medium( DM) for 2,4,6 and 8 days. Smooth muscle specific markers,including α-smooth muscle actin( α-SMA),SM22 and smooth muscle myosin heavy chain( SM-MHC),were detected to insure the successful model by q RT-PCR and Western blot. After 3-day pre-differentiation,ESCs were treated with recombinant Rspo2 protein,overexpression plasmid or sh RNA plasmid for 96 h,and the m RNA and protein expression of smooth muscle markers was detected. To explore the role of Rspo2 on SMC differentiation in vivo,3-day predifferentiated ESCs(106in 50 μLα-MEM) incubated with Rspo2-overexpression plasmid were mixed with 50 μL of Matrigel( Becton Dickinson Labware) and then subcutaneously injected into C57 BL /6J mice. After 12 days,mice were sacrificed and the implants were harvested for immunofluorescence staining,q RT-PCR and Western blot. Furthermore,electrophoretic mobility shift assay( EMSA),chromatin immunoprecipitation assay( Ch IP) and luciferase reporter assay were performed to investigate the transcriptional activity of SMC differentiation related transcription factors,including serum response factor( SRF),myocardin( MYO),myocyte-specific enhancer factor 2C( MEF-2C). Involvement of Rspo2 receptor,leucine-rich repeat-containing,G-protein-coupled receptors( Lgr) 4,5,6,and β-catenin pathway during Rspo2-induced MSC differentiation were also uncovered by overexpression or inhibition of the respective protein. RESULTS: Our resu

关键词： stem Rspo2 promotes embryonic stem cells to differentiate towards smooth muscle cells through LGR5 catenin pathway