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A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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Bio-design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.dos Santos p.J.dijkstra c.Mota J.Bauer H.Ferreira Rui L.Reis N.Neves S.camarero-Espinosa M.B.Baker L.Moroni department of complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal IcVS/3B’s-pT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal pOLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(pcL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight pcL75k at different *** porous three-dimensional(3d)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight pcL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMp-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMScs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMp receptor II(BMpRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density click chemistry Human

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The dallas donation after circulatory death Transplantation Summit:expanding donation after circulatory death procedures through process improvement,broader utilization,and innovation

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Hepatobiliary Surgery and Nutrition 2024年第5期13卷 824-836,I0009页

作者： Michele Finotti Anji Wall Anthony d’Alessandro Gary Schwartz chris Sonnenday david Goldberg Ashish Shah peter Friend Jeff p.Orlowski Greg McKenna Steve Newton Brad Adams William c.chapman Amit Mathur Marwan Abouljoud Tim pruett Amelia Hessheimer James F.Trotter Sumeet K.Asrani Giuliano Testa Simmons Transplant Institute Baylor University Medical CenterDallasTXUSA Hepatobiliary and General Surgery Unit Regional Hospital TrevisoDISCOGUniversity of PaduaPaduaItaly division of Transplantation Department of SurgeryUniversity of Wisconsin School of Medicine and Public HealthMadisonWIUSA department of Thoracic Surgery and Lung Transplantation Baylor University Medical CenterCenter for Advanced Heart and Lung DiseaseDallasTXUSA department of Surgery University of MichiganAnn ArborMIUSA division of digestive Health and Liver diseases Department of MedicineUniversity of Miami Miller School of MedicineMiamiFLUSA department of cardio-Thoracic Surgery Vanderbilt University Medical CenterNashvilleTNUSA Nuffield department of Surgical Sciences University of OxfordOxfordUK LifeShare Transplant donor Services of Oklahoma Oklahoma CityOKUSA Administration Baylor University Medical CenterDallasTXUSA Southwest Transplant Alliance DallasTXUSA department of Surgery/Transplantation Washington UniversitySt.LouisMOUSA division of Transplant Surgery Department of SurgeryMayo Clinic ArizonaAlix School of MedicinePhoenixAZUSA divisions of Transplant and Hepatobiliary Surgery Henry Ford HospitalDetroitMIUSA department of Surgery University of MinnesotaMinneapolisMNUSA General and digestive Surgery Hospital Universitario La PazIdiPAZMadridSpain

despite a significant increase in utilization over the past decade,the number of donation after circulatory death(dcd)organs that are procured and transplanted in the United States(US)remains well below its *** is still room for expansion,as utilizing dcd organs to the fullest extent is currently the most viable solution to the persistent mismatch between supply and demand in *** convened a multidisciplinary transplantation summit to examine various aspects of dcd,with faculty members from around the world with clinical and academic interest in dcd donation and transplantation,including abdominal and cardiothoracic surgeons,organ procurement organization directors,hepatologists,and *** conference focused on identifying barriers to dcd organ utilization and strategies to overcome these *** divide the barriers to dcd utilization into three mains categories:(Ⅰ)policy and process variation;(II)logistical and transportation challenges;and(Ⅲ)higher risk perceptions related to dcd *** each barrier,we proposed a variety of solutions,providing an overview of the status of dcd donation in the US and suggestions on how to increase the use of *** is a specific focus on ex situ machine perfusion,normothermic regional perfusion,and other opportunities to expand dcd utilization without negatively impacting recipient outcomes.

关键词： donation after circulatory death donation(dcd donation) marginal graft donor pool machine perfusion(Mp)

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Leveraging the potential of big genomic and phenotypic data for genome-wide association mapping in wheat

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The crop Journal 2024年第3期12卷 803-813页

作者： Moritz Lell Yusheng Zhao Jochen c.Reif Leibniz Institute of plant Genetics and crop plant Research(IpK) Corrensstraße 3D-06466 SeelandGermany

Genome-wide association mapping studies(GWAS)based on Big data are a potential approach to improve marker-assisted selection in plant *** number of available phenotypic and genomic data sets in which medium-sized populations of several hundred individuals have been studied is rapidly *** these data and using them in GWAS could increase both the power of QTL discovery and the accuracy of estimation of underlying genetic effects,but is hindered by data heterogeneity and lack of *** this study,we used genomic and phenotypic data sets,focusing on central European winter wheat populations evaluated for heading *** explored strategies for integrating these data and subsequently the resulting potential for *** interoperability between data sets was greatly aided by some overlapping genotypes and a linear relationship between the different phenotyping protocols,resulting in high quality integrated phenotypic *** this context,genomic prediction proved to be a suitable tool to study relevance of interactions between genotypes and experimental series,which was low in our *** to expectations,fewer associations between markers and traits were found in the larger combined data than in the individual experimental ***,the predictive power based on the marker-trait associations of the integrated data set was higher across data ***,the results show that the integration of medium-sized to Big data is an approach to increase the power to detect QTL in *** results encourage further efforts to standardize and share data in the plant breeding community.

关键词： Big data Genome-wide association study data integration Genomic prediction Wheat

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Learning about good nutrition with the 5-color front-of-package label"Nutri-Score":an experimental study

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Food Science and Human Wellness 2024年第3期13卷 1195-1200页

作者： Robin c.Hau Klaus W.Lange University of Applied Sciences Landshut Landshut 84036Germany Faculty of Human Sciences University of RegensburgRegensburg 93040Germany

The Nutri-Score is a 5-color front-of-pack nutrition label designed to provide consumers with an easily understandable guideline to the healthiness of food *** impact that the Nutri-Score may have on consumers'choices is unclear since different experimental paradigms have found vastly different effect *** the present study,we have investigated how student participants change a hypothetical personal 1-daydietary plan after a learning phase during which they learn about the Nutri-Scores of the available food *** were instructed to compose a healthy diet plan in order that the question of whether the NutriScore would improve their ability to compose a healthy dietary plan could be investigated,independent of the question of whether they would apply this knowledge in their ordinary *** found a substantial(cohen's d=0.86)positive impact on nutritional quality(as measured by the Nutrient profiling System score of the Food Standards Agency)and a medium-sized(cohen's d=0.43)reduction of energy *** content reduction was larger for participants who had initially composed plans with higher energy *** results suggest that the Nutri-Score has the potential to guide consumers to healthier food *** remains unclear,however,whether this potential will be reflected in real-life dietary choices.

关键词： Nutri-Score Front-of-package label Nudge Nutrition Health

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Breaking paradigms: Long non-coding RNAs forming gene fusions with potential implications in cancer

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Genes & diseases 2024年第3期11卷 311-323页

作者： david Sanchez-Marin Macrina Beatriz Silva-cazares Fany Iris porras-Reyes Rebeca Garcia-Roman Alma d.campos-parra posgrado en ciencias Biologicas Facultad de MedicinaUniversidad Nacional Autonoma de MexicoCiudad de MexicoC.P.04360Mexico Unidad Academica Multidisciplinaria Region Altiplano Universidad Autonoma de San Luis Potost(UASLP)Carretera a Cedral Km 5+600Ejido San Jose de la TrojesMatehualaSan Luis PotosiC.P.78760Mexico Servicio de Anatomia patologica Instituto Nacional de Cancerologia(INCan)Nino JesuisTlalpanCiudaddeMexicoC.P.14080Mexico Instituto de Salud publica Universidad Veracruzana(UV)Av.Dr LuisDr.Castelazo Ayala s/nCol.Industrial AnimasXalapaVeracruzC.P.91190Mexico

Long non-coding RNAs(lncRNAs)are non-coding RNAs longer than 200 nucleotides with dynamic regulatory *** interact with a wide range of molecules such as dNA,RNA,and proteins to modulate diverse cellular functions through several mechanisms and,if deregulated,they can lead to cancer development and ***,it has been described that IncRNAs are susceptible to form gene fusions with mRNAs or other IncRNAs,breaking the paradigm of gene fusions consisting mainly of protein-coding ***,their biological significance in the tumor phenotype is still ***,their recent identification opens a new line of research to study their biological role in tumor-igenesis,and their potential as biomarkers with clinical relevance or as therapeutic *** present study aimed to review the IncRNA fusions identified so far and to know which of them have been associated with a potential *** address the current challenges to deepen their study as well as the reasons why they represent a future therapeutic window incancer.

关键词： cancer Genefusions IncRNAs ncRNAs Therapy

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STcF conceptual design report (Volume 1): physics & detector

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Frontiers of physics 2024年第1期19卷 1-154页

作者： M.Achasov X.c.Ai L.p.An R.Aliberti Q.An X.Z.Bai Y.Bai O.Bakina A.Barnyakov V.Blinov V.Bobrovnikov d.Bodrov A.Bogomyagkov A.Bondar I.Boyko Z.H.Bu F.M.cai H.cai J.J.cao Q.H.cao X.cao Z.cao Q.chang K.T.chao d.Y.chen H.chen H.X.chen J.F.chen K.chen L.L.chen p.chen S.L.chen S.M.chen S.chen S.p.chen W.chen X.chen X.F.chen X.R.chen Y.chen Y.Q.chen H.Y.cheng J.cheng S.cheng T.G.cheng J.p.dai L.Y.dai X.c.dai d.dedovich A.denig I.denisenko J.M.dias d.Z.ding L.Y.dong W.H.dong V.druzhinin d.S.du Y.J.du Z.G.du L.M.duan d.Epifanov Y.L.Fan S.S.Fang Z.J.Fang G.Fedotovich c.Q.Feng X.Feng Y.T.Feng J.L.Fu J.Gao Y.N.Gao p.S.Ge c.Q.Geng L.S.Geng A.Gilman L.Gong T.Gong B.Gou W.Gradl J.L.Gu A.Guevara L.c.Gui A.Q.Guo F.K.Guo J.c.Guo J.Guo Y.p.Guo Z.H.Guo A.Guskov K.L.Han L.Han M.Han X.Q.Hao J.B.He S.Q.He X.G.He Y.L.He Z.B.He Z.X.Heng B.L.Hou T.J.Hou Y.R.Hou c.Y.Hu H.M.Hu K.Hu R.J.Hu W.H.Hu X.H.Hu Y.c.Hu J.Hua G.S.Huang J.S.Huang M.Huang Q.Y.Huang W.Q.Huang X.T.Huang X.J.Huang Y.B.Huang Y.S.Huang N.Hüsken V.Ivanov Q.p.Ji J.J.Jia S.Jia Z.K.Jia H.B.Jiang J.Jiang S.Z.Jiang J.B.Jiao Z.Jiao H.J.Jing X.L.Kang X.S.Kang B.c.Ke M.Kenzie A.Khoukaz I.Koop E.Kravchenko A.Kuzmin Y.Lei E.Levichev c.H.Li c.Li d.Y.Li F.Li G.Li G.Li H.B.Li H.Li H.N.Li H.J.Li H.L.Li J.M.Li J.Li L.Li L.Li L.Y.Li N.Li p.R.Li R.H.Li S.Li T.Li W.J.Li X.Li X.H.Li X.Q.Li X.H.Li Y.Li Y.Y.Li Z.J.Li H.Liang J.H.Liang Y.T.Liang G.R.Liao L.Z.Liao Y.Liao c.X.Lin d.X.Lin X.S.Lin B.J.Liu c.W.Liu d.Liu F.Liu G.M.Liu H.B.Liu J.Liu J.J.Liu J.B.Liu K.Liu K.Y.Liu K.Liu L.Liu Q.Liu S.B.Liu T.Liu X.Liu Y.W.Liu Y.Liu Y.L.Liu Z.Q.Liu Z.Y.Liu Z.W.Liu I.Logashenko Y.Long c.G.Lu J.X.Lu N.Lu Q.F.Lü Y.Lu Y.Lu Z.Lu p.Lukin F.J.Luo T.Luo X.F.Luo Y.H.Luo H.J.Lyu X.R.Lyu J.p.Ma p.Ma Y.Ma Y.M.Ma F.Maas S.Malde d.Matvienko Z.X.Meng R.Mitchell A.Nefediev Y.Nefedov S.L.Olsen Q.Ouyang p.pakhlov G.pakhlova X.pan Y.pan E.passemar Y.p.pei H.p.peng L.peng X.Y.peng X.J.peng K.peters S.pivovarov E.pyata B.B.Qi Y.Q.Qi W.B.Qian Y.Qian c.F.Qiao J.J.Qin J.J.Qin L.Q.Qin X.S.Qin T.L.Qiu J.Rademacker c.F.Redmer H.Y.Sang Anhui University Hefei 230039China Beihang University Beijing 100191China Budker Institute of Nuclear physics Novosibirsk 630090Russia cAS Key Laboratory of Theoretical physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China cavendish Laboratory University of CambridgeJJ Thomson AveCambridge CB30HEUnited Kingdom central china Normal University Wuhan 430079China central South University Changsha 410083China china University of Geosciences Wuhan 430074China china University of Mining and Technology Xuzhou 221116China École polytechnique Fédérale de Lausanne(EpFL) LausanneSwitzerland Fudan University Shanghai 200433China Goethe University Frankfurt D-60325 Frankfurt am MainGermany Guangxi Normal University Guilin 541004China Guangxi Uninversity Nanning 530004China Hangzhou Institute for Advanced Study UCASHangzhou 310024China Hebei Normal University Shijiazhuang 050024China Hebei University Baoding 071002China Hefei University of Technology Hefei 230601China Helmholtz Institute Mainz Staudinger Weg 18D-55099 MainzGermany Henan Normal University Xinxiang 453007China Henan University Kaifeng 475004China High Energy physics center Chung-Ang UniversitySeoul 06974Korea Higher School of Economy 11 pokrovsky Bulvar Moscow 109028Russia Huangshan University Huangshan 245000China Hubei University of Automotive Technology Shiyan 442002China Hunan Normal University Changsha 410081China Hunan University of Science and Technology Xiangtan 411201China Hunan University Changsha 410082China Indiana University BloomingtonIndiana 47405USA Inner Mongolia University Hohhot 010021China Institute of Advanced Science Facilities Shenzhen 518107China Institute of High Energy physics Chinese Academy of SciencesBeijing 100049China Institute of Modern physics Chinese Academy of SciencesLanzhou 730000China Institute of physics Academia SinicaTaipeiTaiwan 11529China Institute of Theoretical physics Chinese Academy of SciencesBeijing 100190China Jilin University Changch

The superτ-charm facility(STcF)is an electron–positron collider proposed by the chinese particle physics *** is designed to operate in a center-of-mass energy range from 2 to 7 GeV with a peak luminosity of 0.5×10^(35) cm^(–2)·s^(–1) or *** STcF will produce a data sample about a factor of 100 larger than that of the presentτ-charm factory—the BEpcII,providing a unique platform for exploring the asymmetry of matter-antimatter(charge-parity violation),in-depth studies of the internal structure of hadrons and the nature of non-perturbative strong interactions,as well as searching for exotic hadrons and physics beyond the Standard *** STcF project in china is under development with an extensive R&d *** document presents the physics opportunities at the STcF,describes conceptual designs of the STcF detector system,and discusses future plans for detector R&d and physics case studies.

关键词： electron–positron collider tau-charm region high luminosity STcF detector conceptual design

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The multi-impurity system in cdSe nanoplatelets:electronic structure and thermodynamic properties

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communications in Theoretical physics 2024年第3期76卷 165-173页

作者： d A Baghdasaryan V A Harutynyan E M Kazaryan H A Sarkisyan Russian-Armenian University H Emin 1230051YerevanArmenia Institute of Applied problems of physics 25 Nersisyan St0014YerevanArmenia

This paper theoretically studies the impurity states and the effects of impurity concentration and configuration on the optical,electrical,and statistical properties of cdSe nanoplatelets(NpLs).An image charge-based model of electron-impurity interaction is *** charge-carrier energy spectra and corresponding wave functions depending on the impurity number and configuration are *** electron binding energies are calculated for different NpL *** is shown that the image charge-based interaction potential that arises due to the dielectric constants mismatch is much stronger than the interaction potential that does not take such a mismatch into ***,it is demonstrated that the binding energies are increasing with the number of *** calculate the canonical partition function using the energy levels of the electron,which in turn is used to obtain the mean energy,heat capacity,and entropy of the non-interacting electron *** thermodynamic properties of the non-interacting electron gas that depend on the geometric parameters of the NpL,impurity number,configuration,and temperature are studied.

关键词： nanoplateletes thermodynamic properties impurity states

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Endoscopic ultrasound-guided portal pressure gradient identifies patients with previously undiagnosed clinically significant portal hypertension

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portal Hypertension & cirrhosis 2024年第2期3卷 67-74页

作者： Jerome c.Edelson Scott F.d.Edelson John G.Quiles cyrus V.Edelson Eric M.Montminy christian L.Horn John p.Magulick don c.Rockey department of Gastroenterology and Hepatology Brooke Army Medical CenterFort Sam HoustonTexasUSA department of Medicine Uniformed Services University of the Health SciencesBethesdaMarylandUSA department of Medicine Brooke Army Medical CenterFort Sam HoustonTexasUSA John H.Stroger Jr.Hospital of cook county ChicagoIllinoisUSA digestive disease Research center Medical University of South CarolinaCharlestonSouth CarolinaUSA

Aims:portal hypertension(pH)is a complication of cirrhosis that leads to hepatic *** the portal pressure gradient(ppG)provides valuable information for disease assessment,staging,and *** this study,we aimed to report the utilization of endoscopic ultrasound(EUS)-guided ppG(EUS-ppG)measurements in a real-world ***:This retrospective analysis included patients at a tertiary care center who underwent EUS-ppG between February 2021 and May *** most common indication was to establish or exclude the diagnosis of cirrhosis,followed by an assessment of surgical risk in the setting of suspected *** demographic and clinical data were collected,and statistical analysis was performed using the Student's t ***:Eighteen patients underwent *** success was achieved in 17 of the 18 *** complications were *** mean scores of child-pugh,Model for End-Stage Liver disease-Sodium,and Fibrosis-4 scores were 5.1±0.5,9.0±3.0,and 3.7±4.5,*** the 18 patients,two presented with esophageal varices and six with portal hypertensive *** mean ppG was 5.8±4.5 mmHg,and five patients exhibited clinically significant pH(cSpH).Of the 18 patients,15 underwent concurrent EUS-liver biopsy,which was 100%successful and provided diagnostic histology for all *** ppG data led to changes in the clinical management of 17 ***:EUS-ppG is safe and technically *** child-pugh score did not correlate well with the presence of cSpH;however,ppG measurements resulted in changes in the management of almost all *** studies correlating EUS-ppG with other assessments of liver diseases are required.

关键词： EUS portal hypertension portal pressure gradient

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Effects of interlayer spacing and applied pressure on the lanthanide transport in MoS2-based two-dimensional channels

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International Journal of Smart and Nano Materials 2024年第3期15卷 579-592页

作者： Qinsi Xiong chong Liu George c.Schatz department of chemistry Northwestern UniversityEvanstonILUSA pritzker School of Molecular Engineering University of ChicagoChicagoILUSA

Rare-earth elements(REEs)are critical to modern industry but difficult to separate due to their subtle and monotonic changes in physico-chemical ***2-based two-dimensional(2d)materials offer novel opportunities for enhancing REE separation,exhibiting a distinct volcano-shaped transport performance distribution that peaks at Sm3+.However,the specific contributions of thermodynamic and kinetic factors to ion transport within 2d confinement remain *** this study,we conducted a series of non-equilibrium all-atom molecular dynamics(Md)simulations to explore the effects of interlayer spacing and external pressure on the transport of lanthanide ions inÅ-scale acetate functionalized 2d MoS2(MoS2-cOOH)*** examined ion entry and permeation rates,water flux,dehydration,and binding *** simulation results reveal that the transport trends of lanthanide ions are jointly driven by the dehydration degree and the relative-binding strengths of ions to water and to the acetate within the 2d ***,the dehydration pattern of lanthanide ions during permeation is closely linked to kinetic ***,this study provides a detailed atomistic understanding of the mechanisms under-lying lanthanide ion transport under *** findings point to the significant potential for tuning confinement and chemical functionalization withinÅ-scale channels for more efficient REE separation.

关键词： MoS_(2) 2d channels Lanthanide ions ion transport nanofluidics

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A combined experimental and dEM investigation of grain interlocking in sheared granular assemblies

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particuology 2024年第7期90卷 436-451页

作者： Rui Lu Qiang Luo Tengfei Wang david p.connolly Tao Xie School of civil Engineering Southwest Jiaotong UniversityChengdu 610031China Key Laboratory of High-Speed Railway Engineering(Southwest Jiaotong University) Ministry of EducationChengdu 610031China School of civil Engineering University of LeedsLeeds LS29JTUK department of civil Engineering McMaster UniversityHamiltonONCanada

compacted granular material,integral to geotechnical engineering,undergoes translation,rotation,and interlocking when subject to shear displacements or external *** present study focuses on the interlocking of heterogeneous granular materials,a complex behavior influenced by gradation,compaction,and varying particle geometry,and has consequently received limited attention in existing *** address this research gap,we conducted an analysis on the effect of grain interlocking on the shear resistance of granular assemblies,using a combination of laboratory testing and the discrete element method(dEM).Initially,large-scale direct shear tests were conducted on gravel−sand mixes with varying degrees of compaction and normal *** of the mixes also underwent subsequent shear reversal to explore the differences in grain interlocking between the two shearing processes on the shear *** analyzing the laboratory results,a mesoscopic scale investigation was performed by replicating the test using discrete element *** facilitate this,granular particle geometries were measured using 3d laser scanning based on the physical lab ***,based on these scans,discrete element R-block and ball models were utilized to construct both the coarse and fine particles within the *** vibro-compaction was employed to regulate the degree of *** results indicate that an increase in vertical pressure,coupled with a zero dilatancy angle,results in a rising stress ratio,indicative of grain *** interlocking exhibits a positive correlation with both the coarse content and the degree of compaction,and varies depending on the shear *** interlocking progresses,the shear band,induced by particle movement,expands and is associated with reduced particle rotation near the shear *** study further reveals a consistent positive correlation between interlocking and the principal orientation angle of strong normal c

关键词： Granular material Interlocking Shear resistance direct shear discrete element method Vibro-compaction

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