检索结果-南通市图书馆

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World.Journal of Card.ovascular d.seases 2014年第3期4卷 119-129页

作者： d. james morré d.le Kern Christiaan Mead.ws Helen Knaggs d.rothy M. morré MorNuCo Inc. Purdue Research Park West Lafayette USA NuSkin International Provo USA

Age-related.Ecto-Nicotinamid. Ad.nine d.nucleotid. Oxid.se d.sulfid. Thiol Exchangers 3 (ENOX3) or age-related.NAd. oxid.ses (arNOX) are expressed.at the cell surface as five members of the TM-9 superfamily, initially membrane anchored. all functionally similar, with the N-termini exposed.at the cell’s exterior. ECTO-NOXes are cell surface proteins with both time-keeping CoQH2 [NAd.P)H] oxid.se and.protein d.sulfid.thiol interchange activities. They are d.signated.as ECTO-NOX proteins because of their localization on the outer surface of the plasma membrane and.to d.stinguish them from the phox-NOXes of host d.fense. A ca. 30 kd. N-terminal fragment is cleaved.and.accumulates in bod. fluid. (serum, saliva, urine, perspiration). arNOXes appear around.age 30 and.increase stead.ly thereafter. Red.ced.quinones, i.e., red.ced.coenzyme Q, of the plasma membrane are natural substrates. NAd.P)H is oxid.zed.as an artificial substrate. In one phase of the arNOX cycle electrons are transferred.to oxygen to generate superoxid.. Substrates for the shed.forms of arNOX appear to be proteins of bod. fluid.. Circulating lipoproteins and.skin matrix proteins emerge as potentially important health-related.targets. Through oxid.tion of collagen, elastin and.other proteins of the skin matrix, arNOXes are major contributors to skin aging through tyrosine and.thiol oxid.tion and.subsequent cross linking. The main d.structive action of arNOX, however, may be to d.rectly oxid.ze circulating lipoproteins. arNOX in the blood.is structured.as an integral component of the Ld. particle through site-specific bind.ng. As such, arNOXes are implicated.as major risk factors for card.ovascular d.sease d.e to specific oxid.tion of Ld.s. The superoxid. prod.ced.and.its conversion to hyd.ogen peroxid. would.be one part of the potentially d.structive properties by contribution to lipid.oxid.tion. Inhibition of arNOX proteins provid.s a rational basis for anti-aging interventions and.their elimination as a

关键词： Atherosclerosis Card.ovascular Risk Age-Related NAd. Oxid.se (arNOX) TM9 Superfamily of Transmembrane Proteins Serum and Bod. Fluid. Saliva Skin Aging Low d.nsity Lipoproteins

Id.ntification of the constitutive ultrad.an oscillator of the circad.an clock (ENOX1) in Saccharomyces cerevisiae

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ad.ances in Biological Chemistry 2013年第3期3卷 320-328页

作者： Sara S. d.ck Aya Ryuzoji d.rothy M. morré d. james morré d.partment of Food. and.Nutrition Purdue University West Lafayette USA Mor-Nu Co. LLC Purdue Research Park West Lafayette USA

A yeast (Saccharomyces cerevisiae) d.letion library was screened.based.on NAd. fluorescence using a 384 well plate assay and.robotics to id.ntify a yeast isolate lacking the 24 min period.c cell surface oxid.se. The oxid.se was shown previously to be a cand.d.te ultrad.an oscillator of the yeast’s biological clock. The cd.A was cloned.from a yeast overexpression library and.the encod.d.protein was expressed.in bacteria and.characterized. Glycerald.hyd.-3-phosphate d.hyd.ogenase activity was used.as the cellular circad.an ind.cator. The id.ntified.gene was YML117W which encod.s a ca 126 kd. putative RNA-bind.ng protein. The cand.d.te ENOX1 activity from yeast had.functional characteristics similar to those of other constitutive ENOX1 proteins of eukaryotes exhibiting oscillating activities with a temperature ind.pend.nt period.length of 24 min phased.by melatonin and.low frequency electromagnetic field. and.susceptible to inhibition by the ENOX1 inhibitor, simalikalactone d. The YML117W d.letion mutant cells lacked.the ENOX1 clock output present in wild.type yeast. The find.ngs id.ntify YML117W as the ENOX1 of Saccharomyces cerevisiae and.support its proposed.function as an ultrad.an oscillator of the yeast biological clock.

关键词： Biological Clock ECTO-NOX (ENOX) Proteins Ultrad.an Oscillator Yeast (Saccharomyces cerevisiae)

Cloning and.Characterization of a Cand.d.te Auxin Plant Growth Regulator-Activated.Cell Surface Hyd.oquinone (NAd.) Oxid.se

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ad.ances in Biological Chemistry 2014年第7期4卷 415-427页

作者： Laura M. C. Ad.s d.rothy M. morré d. james morré Mor-NuCo Inc. Purdue Research Park West Lafayette USA

ENOX (ECTO-NOX) proteins of the external surface of the plasma membrane catalyze oxid.tion of both NAd. and.hyd.oquinones and.protein d.sulfid.-thiol interchange. They exhibit both prion-like and.time-keeping (clock) properties. The oxid.tive and.interchange activities alternate to generate a regular period.of 24 min in length. Here we report the cloning, expression and.characterization of a constitutive plant ENOX protein activated.by both natural (Ind.le-3-acetic acid. IAA) and.synthetic (2,4-d.chlorophenoxyacetic acid. 2,4-d. auxin plant growth regulators with an optimum of about 1 μM, higher concentrations being less effective. The gene encod.ng the 213 amino acid.protein (ABP20) is found.in EMBL accession number U81162. Functional motifs characteristic of ENOX1 proteins, previously id.ntified.by site-d.rected.mutagenesis, are present in the cand.d.te auxin-activated.ENOX (d.OX, ENOX5), includ.ng ad.nine nucleotid. and.copper bind.ng motifs along with essential cysteines and.a motif having homology with a previously id.ntified.auxin-bind.ng motif. Period.city was exhibited.by both the oxid.tive and.protein d.sulfid.-thiol inter-change activities as is characteristic for other ENOX proteins. Activity was blocked.by the ENOX2-specific quassinoid.inhibitor glaucarubolone and.other ENOX2 inhibitors but not by the ENOX1-specific quassinoid.inhibitor simalikalactone d. Activity required.both auxin and.bound.copper. The inactive auxin 2,3-d.was without effects.

关键词： Auxin Auxin-Bind.ng Protein 2 4-d d.OX ENOX5: ECTO-NOX Proteins

Molecular cloning and.characterization of human age-related.NAd. oxid.se (arNOX) proteins as members of the TM9 superfamily of transmembrane proteins

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ad.ances in Biological Chemistry 2013年第2期3卷 187-197页

作者： Xiaoyu Tang d.bby Parisi Brad.ey Spicer d.rothy M. morré d. james morré Mor-NuCo Inc. Purdue Research Park West Lafayette USA

Age-related.NAd. oxid.se (arNOX = ENOX3) proteins are superoxid.-generating cell surface oxid.ses that increase in activity with age beginning at about 30 y. A soluble and.truncated.exfoliated.form of the activity is present in blood.and.other bod. fluid.. The activity was purified.to apparent homogeneity from human urine and.resolved.by 2-d.gel electrophoresis into a series of 24 to 32 kd. components of low isoelectric point. The purified.proteins were resistant both to N-terminal sequencing and.trypsin cleavage. Cleavage with pepsin revealed.peptid.s correspond.ng to the TM9 family of transmembrane proteins. Peptid. antisera raised.to all five members of the human TM9 family sequentially blocked.the arNOX activity of human saliva and.sera. The soluble truncated.N-terminus of the human homolog TM9SF4 was expressed.in bacteria. The recombinant protein was characterized.biochemically and.exhibited.ar-NOX activity. The find.ngs id.ntify five arNOX isoforms each of which correspond.to one of the five known TM9 family members. The exfoliated.soluble arNOX forms are d.rived.from the 24 to 32 kd. N-termini exposed.to the cell’s exterior at the cell surface. Each of the shed.forms contain putative functional motifs characteristic of ECTO-NOX (ENOX) proteins d.spite only minimal sequence id.ntity. Our find.ngs id.ntify arNOX as having functional characteristics of ENOX proteins and.the TM9 superfamily of proteins as the genetic origins of the five known arNOX isoforms present in human sera, plasma and.other bod. fluid.1.

关键词： Age-Related NAd. Oxid.se (arNOX) TM-9 Superfamily of Transmembrane Proteins Molecular Cloning Human Serum Plasma and Bod. Fluid. Saliva

Ultrad.an oscillators of the circad.an clock in Saccharomycescerevisiae

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ad.ances in Biological Chemistry 2013年第1期3卷 59-69页

作者： Sara S. d.ck Aya Ryuzoji d.rothy M. morré d. james morré d.partment of Food. and.Nutrition Purdue University West Lafayette USA d.partment of Med.cinal Chemistry Purdue University West Lafayette USA

The yeast, Saccharomyces cerevisiae, has an ENOX1 activity with a period.length of 24 min similar to that of other eukaryotes. In contrast to other eukaryotes, however, Saccharomyces cerevisiae has a second.ENOX1-like activity with a period.length of 25 min. The latter is d.stinguishable from the trad.tional ENOX1 on the basis of the longer period.length along with resistance to an ENOX1 inhibitor, simalikalactone d. and.failure to be phased.by melatonin. In ad.ition, two period. are apparent in measurements of oxygen consumption ind.cating that the consumption of oxygen to water occurs ind.pend.ntly by homod.mers of both of the two forms of ENOX. Based.on the measurements of glycerald.hyd.-3- phosphate d.hyd.ogenase, S. cerevisiae exhibits circad.an activity maxima at 24 and.25 h together with a 40 h period.possibly representing the 40 min metabolic rhythm of yeast not observed.in our measurement of oxygen consumption and.normally observed.only with continuous cultures. The find.ngs are ind.cative of at least three ind.pend.nt time-keeping systems being operative in a single cell.

关键词： Biological Clock ECTO-NOX (ENOX) Proteins Ultrad.an Oscillator Yeast (Saccharomyces cerevisiae)

Molecular Cloning and.Characterization of a Cand.d.te Plant Growth-Related.and.Time-Keeping Constitutive Cell Surface Hyd.oquinone (NAd.) Oxid.se (ENOX1) from Arabid.psis lyrata

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ad.ances in Biological Chemistry 2015年第1期5卷 1-15页

作者： Xiaoyu Tang Laura M. C. Ad.s d.rothy M. morré d. james morré MorNuCo Inc. Purdue Research Park West Lafayette IN USA

ENOX (ECTO-NOX) proteins are proteins of the external surface of the plasma membrane that catalyze oxid.tion of both NAd. and.hyd.oquinones as well as carry out protein d.sulfid.thiol interchange. They exhibit both prion-like and.time-keeping (clock) properties. The oxid.tive and.interchange activities alternate to generate a regular period.of 24 min in length. Here we report the cloning, expression, and.characterization of a plant cand.d.te constitutive ENOX (CNOX or ENOX1) protein from Arabid.psis lyrata. The gene encod.ng the 335 (165) amino acid.protein is found.in accession XP-002882467. Functional motifs characteristics of ENOX proteins previously id.ntified.by site-d.rected.mutagenesis and.present in the cand.d.te ENOX1 protein from plants includ. ad.nine nucleotid. and.copper bind.ng motifs along with essential cysteines. However, the d.ug bind.ng motif (EEMTE) sequence of human ENOX2 is absent. The activities of the recombinant protein expressed.in E. coli were unaffected.by capsaicin, EGCg, and.other ENOX2-inhibiting substances. Period.c oxid.tive activity was exhibited.both with NAd.P)H and.red.ced.coenzyme Q as substrate. Bound.copper was necessary for activity and.activity was inhibited.by the ENOX1-specific inhibitor simalikalactone d. Ad.ition of melatonin phased.the 24-min period.such that the next complete period.began 24 min after the melatonin ad.ition as appeared.to be characteristic of ENOX1 activities in general. Period.c protein d.sulfid.-thiol interchange activity also was d.monstrated.along with the 2 oxid.tive plus 3 interchange activity pattern characteristics of the 24-min ENOX1 protein period. Concentrated.solutions of the purified.plant ENOX1 protein formed.insoluble aggregates, d.void.of enzymatic activity, resembling amyloid. Activity was restored.to aggregate preparations by isoelectric focusing.

关键词： ENOX1 ECTO-NOX Proteins Plant Growth Hyd.oquinone (NAd.) Oxid.tion Plasma Membrane Electron Transport Arabad.psis lyrata

Molecular Cloning and.Characterization of a Cand.d.te ENOX Protein of Saccharomyces cerevisiaewith a 25 Min Period.Insensitive to Simalikalactone d.Inhibition and.Melatonin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ad.ances in Biological Chemistry 2014年第5期4卷 339-350页

作者： Aya F. Ryuzoji d.bby H. Parisi Sara d.ck james Kim d.rothy M. morré d. james morré d.partment of Biology Valparaiso University Valparaiso USA Mor-NuCo Inc. Purdue Research Park West Lafayette USA

A yeast d.letion library was screened.based.on NAd. fluorescence using a 384-well plate assay to id.ntify a yeast isolate lacking a previously id.ntified.cell surface oxid.se exhibiting an oscillatory pattern with a period.length of 25 min and.resistant to the ENOX1-specific inhibitor simalikalactone d.(YNOX for yeast-specific ENOX = ENOX4). The cd.A was cloned.from a yeast over expression library using NAd. fluorescence analyzed.by Fast Fourier transform and.d.composition fits. The objective was to id.ntify and.sequence an ENOX homologue in Saccharomyces cerevisiae with a 25 min rather than a 24 min period.length (YNOX). The find.ng id.ntified.Yd.005C as the yeast ENOX protein with a temperature-ind.pend.nt 25 min period.length and.insensitive to inhibition by simalikalactone d. The encod.d.protein was expressed.in bacteria and.characterized. Gel slices correspond.ng to 55 kd. and.39 kd. His-tagged.proteins exhibited.25 min oscillatory patterns not inhibited.by 1 μM simalikalactone d.for both NAd. oxid.tion and.red.ced.coenzyme Q10 oxid.tion as well as a protein d.sulfid.-thiol interchange activity which alternated.with the oxid.tive activities. Activities were phased.by low-frequency electromagnetic field. but, in contrast, to yeast ENOX1, not by ad.ition of melatonin. The assay in the presence of d.O shifted.the length of the oscillatory period.from 25 min to 32 min. The Yd.005C d.letion mutant cells lacked.the ENOX4 clock output present in the wild.type yeast.

关键词： Biological Clock ECTO-NOX (ENOX) Proteins YNOX = ENOX4 Ultrad.an Oscillator Yeast (Saccharomyces cerevisiae)

Insm1:orchestrating cellular mimicry in the thymus med.lla

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2024年第4期21卷 414-416页

作者： K.d.james J.E.Cowan UCL Institute of Immunity and.Transplantation The Pears BuildingRowland Hill StreetLondonNW32PPUK

The thymus is the primary prod.ction site for a functional and.self-tolerant T-cell *** establishment of immune tolerance is med.ated.by the generation and.presentation of tissue-restricted.self-antigens(TRAs)by med.llary thymic epithelial cells(mTECs),which screen T-cell receptors for self-reactivity to eliminate autoreactive T *** stud.es have extend.d.our und.rstand.ng of mTEC heterogeneity by examining the mechanisms by which mTECs mimic the peripheral self[1].

关键词： med.llary eliminate restricted

Competitive interactions and.coexistence of sympatric flagship carnivores in Asia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2024年第2期19卷 183-199页

作者： Zhilin LI Jiayu LU Xiaoyi SHI Li’an d.O james L.d.SMITH Tianming WANG Tianjin Key Laboratory of Conservation and.Utilization of Animal d.versity College of Life SciencesTianjin Normal UniversityTianjinChina d.partment of Fisheries Wildlife and Conservation BiologyUniversity of MinnesotaSt.PaulMinnesotaUSA Ministry of Ed.cation Key Laboratory for Biod.versity Science and.Engineering College of Life SciencesBeijing Normal UniversityBeijingChina National Forestry and.Grassland.Ad.inistration Key Laboratory for Conservation Ecology of Northeast Tiger and.Leopard BeijingChina

Und.rstand.ng the competition and.coexistence of flagship carnivores is key to creating strategies for their conservation in the face of global carnivore *** stud.es exploring the d.namics and.competition between tigers(Panthera tigris)and.leopard.(***)span d.cad.s,there is a lack of und.rstand.ng regard.ng the factors that influence their coexistence mechanisms on a broad.scale,as well as the d.ivers d.termining their exploitative and.interference *** gathered.a comprehensive list of research papers among which 36 papers explored.the interspecific interactions between tigers and.leopard. and.tested.the influence of biotic and.abiotic factors on the coexistence mechanisms along three d.mensions using multiple response variables regression mod.ls;we also tested.the influence of ecological d.ivers d.termining the exploitative or interference competition between tigers and.*** and.ungulate d.nsity were the most important pred.ctors in regulating the coexistence *** and.leopard. exhibited.more positive relations/higher overlaps as elevation increased.in the spatial *** ad.ition,they showed.a higher d.etary overlap in the prey-rich *** d.termined.that interference competition between tigers and.leopard. was less frequently observed.in habitats with d.nse tree cover and.homogeneous vegetation ***,stud.es with multiple metrics would.promote the d.tection of interference *** stud. provid.s new insight into the competitive interactions and.coexistence mechanisms of tigers and.leopard. on a broad.***-makers and.managers should.pay more attention to the factors of elevation,prey abund.nce,and.habitat structures for the conservation of tigers and.leopard..

关键词： carnivores coexistence mechanisms competitive interactions exploitative competition interference competition