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New rat to mouse xenograft transplantation of endometrium as a model of human endometriosis

Animal Models and Experimental Medicine 2021年第3期4卷 268-277页

作者： Amir Abdolmaleki Cyrus Jalili Kamran Mansouri Mitra Bakhtiari Department of Anatomical Sciences Faculty of MedicineKermanshah University of Medical SciencesKermanshahIran Medical Biology Research Center Health Technology InstituteKermanshah University of Medical SciencesKermanshahIran

Background:Endometriosis can lead to infertility.Since there is no definitive treat-ment for endometriosis,animal modelling seems necessary to examine the possible treatments.Mouse endometrium cannot be separated for endometriosis induc-tion.In addition,transplantation of uterus into the abdominal viscera to induce endometriosis causes organ damage.In this study,we defined a new model of en-dometriosis leading to separability of endometrium and a safe anatomical region for transplantation.Methods:Forty female mice were allocated to 5 groups:1,sham;2,allograft uterus transplantation of mice to anterior abdominal wall of mice;3,allograft uterus trans-plantation of mice to mesentery of mice;4,xenograft endometrial transplantation of rat to anterior abdominal wall of mice;5,xenograft endometrial transplantation of rat to mesentery of mice.Adult female rats with a previous pregnancy experience were selected and placed in the vicinity of male rats for 2 weeks to induce estrogen secre-tion and increase endometrial thickness.Results:In the 4th group of animals,compared to sham,the peritoneal concentrations of VEGF-A,TNF-α,NO,MDA,and serum levels of CA-125 and IL-37 were increased and total body weight was decreased,while weight and size of endometrial lesions were increased significantly(P<.05).Genes expression of HOXA10 and HOXA11 were decreased significantly(P<.05)in groups 2 and 4 compared to sham.Conclusions:Xenograft transplantation of endometrium from rat to anterior abdomi-nal wall of mice can potentially mimic human endometriosis morphologically,histo-logically,and genetically.

关键词： endometriosis endometrium mice rat xenograft transplantation

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Royal-jelly-based apitherapy can attenuate damages to male reproductive parameter following nicotine administration

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Animal Models and Experimental Medicine 2022年第2期5卷 133-140页

作者： Mahsa Nazar-Zadeh Cyrus Jalili Amene Nikgoftar Fathi Ali Ghanbari Mitra Bakhtiari Department of Anatomical Sciences Medical SchoolKermanshah University of Medical SciencesKermanshahIran Medical Biology Research Center Kermanshah University of Medical SciencesKermanshahIran Department of Anatomical Sciences Medical SchoolArak University of Medical SciencesArakIran

Background:Nicotine administration can generate severe oxidative stress and lipid peroxidation.Royal jelly,with its antioxidant properties,acts as a scavenger of reactive oxygen species.This study describes the apitherapy effects of royal jelly on testicular damage following nicotine administration.Methods:Forty-eight male BALB/c mice were divided into 8 groups(n=6):saline,3 different doses of royal jelly(100,150,and 200 mg/kg body weight(BW)per day),nicotine(1.5 mg/kg),and 3 different groups of Nic+Roy(1.5 mg/kg of Nic+100,150,and 200 mg/kg BW per day of royal jelly).Nicotine was administrated intraperitoneally,and royal jelly was prescribed orally for 10 consecutive days.Serum levels of hormones(testosterone,luteinizing hormone,and follicle-stimulating hormone),total antioxidant capacity,nitric oxide(NO)status,malondialdehyde levels,sperm DNA fragmentation,sperm parameters,histopathological changes(H&E staining),immunohistochemistry against apoptotic proteins,and gene expression of Bcl-2,p53,Caspase-3,and Nrf2(real-time PCR)were assessed to evaluate the molecular and histological changes.Results:Hormone levels,sperm parameters,and status of antioxidants were decreased significantly(p<.05)following nicotine administration.Moreover,royal jelly treatment normalized hormonal and antioxidant characteristics,decreased apoptotic gene expression,increased Nfr2 gene expression,and restored histopathological alteration to the physiological status significantly(p<.05).Conclusion:Royal jelly upregulates the antioxidant status,inhibits the mitochondrialdependent apoptosis pathway,and increases the rate of proliferation.This therapeutic agent effectively protected the testis against nicotine-associated damages by antioxidant and anti-apoptotic effects.

关键词： antioxidant apitherapy apoptosis damage male nicotine reproduction royal jelly testis

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Genistein Improves Liver Damage in Male Mice Exposed to Morphine

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Chinese Medical Journal 2018年第13期131卷 1598-1604页

作者： Mohammad Reza Salahshoor Shiva Roshankhah Payman Hosseni Cyrus Jalili Department of Anatomical Sciences University of Kermanshah School of Medicine Kermanshah Taghbostan *** Iran

Background： Morphine is commonly used to treat severe pain. This substance is significantly metabolized in the liver and causes disturbing effects. Genistein is an isoflavone and has antioxidant properties. The aim of this study was to evaluate the effects of genistein against morphine damages on mouse liver. Methods： Between May 2017 and March 2018, 48 male mice were divided into six groups （n = 8 in each group）. Various doses of genistein （25 and 50 mg/kg） and morphine plus genistein （25 and 50 mg/kg） were administered intraperitoneally to 48 male mice for 20 consequent days. Aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, alkaline phosphatase （ALP）, serum nitric oxide （NO） levels, liver weight, and the diameter of hepatocytes and central hepatic vein were studied and compared using one-way analysis of variance. Results： Morphine administration significantly increased the mean diameter of the central hepatic vein （22.76 ± 1.9 μm vs. 15.04 ± 0.60μm, x^2= 21.814, P = 0.001） and hepatocytes （3.03 ± 0.10 μm vs. 1.10 ± 0.05 μm, x^2 = 9.873, P = 0.001） respectively, blood serum NO level （38.00% ± 2.09% vs. 18.72% ±4.40%, x^2 = 20.404, P 〈 0.001 ）, liver enzyme level （AST： 111.80 ± 5.10 ng/ml vs. 81.93 ±2.20 ng/ ml, x^2 = 32.201, P 〈 0.0001; ALT： 45.14 ± 4.10 ng/ml vs. 35.49 ± 2.50 ng/ml, x^2= 18.203, P 〈 0.0001; and ALP： 3.28 ± 0.20 ng/ml vs. 2.14± 0.10, x^2= 5.04, P 〈 0.0001, respectively）, and decreased liver weight （18.50 ± 0.90 g vs. 27.15 ± 0.50 g, x^2 = 22.415, P=0.001 ） compared to saline group （0.535±3.750, P 〈 0.0001）. However, administration of genistein plus morphine significantly enhanced liver weight （25 mg/kg： 21.15 ±2.13 g vs. 18.50 ±0.90 g, x^2= 19.251 P 〈 0.0001 ; 50 mg/kg： 21.20 ±1.00 g vs. 18.5± 0.9 g, x^2= 19.502, P 〈 0.0001, respectively） and reduced the mean diameter of hepatocyte （25 mg/kg： 2.17±0.30 μm vs. 3.03 ± 0.10 μm, x^2 = 22.780, P =0.001 ; 50 mg/kg： 2.01± 0.20 μm vs. 3.03 ±0.10 μm x^2 = 7.120, P = 0.001, respectively）, central hepatic vein （25 mg/kg： 19.53 ± 1.00 μm vs

关键词： Genistein Liver Damage Morphine

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Effects of Carbophos on Rat Testopathy Following Resveratrol Administration:An Experimental Study

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Reproductive and Developmental Medicine 2019年第4期3卷 222-229页

作者： Cyrus Jalili Shiva Roshankhah Azita Faramarzi Mohammad Reza Salahshoor Medical Biology Research Center Kermanshah University of Medical SciencesKermanshahIran Department of Anatomical Sciences Medical SchoolKermanshah University of Medical SciencesKermanshahIran

Objective:Carbophos(CAR)is an organophosphate that is most capable of producing free radicals and inducing alteration in some of the male reproductive parameters.Resveratrol(RES)is an herbal polyphenol and exerts beneficial antioxidant effects.This study aimed to evaluate the effects of RES against CAR-induced alteration in reproductive parameters of male rats.Methods:In this experimental study,48 male rats were randomly assigned to eight groups:Group 1:Normal control(saline)group;Group 2:CAR control(250 mg/kg)group;Groups 3-5:2,8,and 20 mg/kg RES,respectively,groups;Groups 6-8:CAR plus 2,8,and 20 mg/kg RES,respectively,groups.The agents were administered intraperitoneally and by gavage daily for 65 days.Sperm parameters,testis malondialdehyde(MDA)levels,total antioxidant capacity,testosterone levels,and germinal layer height were evaluated and compared among these groups.Results:No significant alterations were observed in RES groups(with different doses)compared to the normal control group(P>0.05).The levels of all parameters except MDA(which decreased)significantly increased in the CAR plus RES(with different doses)groups compared to the CAR control group(P<0.01).Conclusions:RES attenuates the toxic effect of CAR on some of the male reproductive parameters.

关键词： Carbophos Resveratrol Testopathy

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Falcaria vulgaris extract attenuates diabetes-induced kidney injury in rats

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Asian Pacific Journal of Tropical Biomedicine 2019年第4期9卷 150-157页

作者： jalili cyrus Roshankhah Shiva Salahshoor Mohammad Reza Medical Biology Research Center Kermanshah University of Medical Sciences Department of Anatomical Sciences Medical School Kermanshah University of Medical Sciences

Objective: To assess the effects of Falcaria vulgaris(F. vulgaris) as an antioxidant on damage to kidney of diabetic rats. Methods: Diabetic rats were established via streptozotocin(60 mg/kg). Various doses of F. vulgaris extracts(50, 100 and 150 mg/kg) and streptozotocin + F. vulgaris extracts were administered via intraperitoneal(i.p) injection to 48 rats(n=8 per group) for 28 d. Subsequently, ferric ion reducing antioxidant power(FRAP) of renal tissue, thiobarbituric acid reactive species, blood glucose concentrations, insulin, nitrite oxide, the weight of animals, glomeruli characteristics and kidney function were evaluated. Results: Compared with the control rats, diabetic rats showed significant increase in malondialdehyde, blood urea nitrogen, creatinine, blood glucose, nitrite oxide contents in renal tissues, and glomerular diameter. Furthermore, tissue FRAP level, body weight, number of glomeruli and plasma insulin were markedly reduced in diabetic rats when compared with the control group(P < 0.05). However, in all F. vulgaris and F. vulgaris + streptozotocin groups, malondialdehyde level, blood urea nitrogen, creatinine, glomerular diameter, nitrite oxide, and glucose levels were decreased significantly; meanwhile, tissue FRAP level, body weight, glomeruli number and insulin serum level were increased, compared to the control diabetic group(P < 0.05). Conclusions: F. vulgaris extract alleviates renal damage in diabetic rats.

关键词： Antioxidant Diabetes Falcaria vulgaris Kidney

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Improvement of Phaseolus vulgaris on breastfeeding in female rats

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Asian pacific Journal of Reproduction 2019年第2期8卷 70-74页

作者： Shiva Roshankhah Cyrus Jalili Mohammad Reza Salahshoor Department of Anatomical Sciences Medical SchoolKermanshah University of Medical SciencesKermanshahIran Medical Biology Research Center Department of Anatomical SciencesKermanshah University of Medical SciencesKermanshahIran

Objective: To evaluate effect of Phaseolus vulgaris (P. vulgaris) on the breastfeeding in female rats. Methods: This experimental study was done from May 2018 to December 2018 in the Anatomical Department of Medical School in Kermanshah University of Medical Sciences in Iran. In this study, after one-week adaptation and fertilization by male, 40 female rats within 20 days of pregnancy (on average, every mother had 10 newborns) were equally separated into four groups (animals were administrated after delivery of offspring). Group 1 was control group receiving normal saline interaperitoneally, and groups 2, 3, 4 were treatment groups receiving the dose of 20, 50, 100 mg/kg of P. vulgaris interaperitoneally respectively once a day for 60 days. The prolactin hormone was measured by radio immune assay, number and diameter of alveoli via histological and morphometrical examinations, and receptor prolactin gene expression by real-time polymerase chain reaction method. Results: P. vulgaris significantly improved alveoli's number and diameter, prolactin hormone and receptor prolactin expression when compared to the control group (P<0.001). Conclusions: P. vulgaris is helpful to improve the breastfeeding parameters of rats' mammary glands.

关键词： Phaseolus vulgaris Breastfeeding Animal

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