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Analysis of phosphorylation sites on autophagy proteins

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Protein & Cell 2015年第9期6卷 698-701页

作者： Wenzhi Feng Wenhao Zhang Hui Wang Lili Ma Di Miao Zexian liu yu Xue Haiteng Deng Li yu PTN Program College of Life SciencePeking UniversityBeijing100871China Center for Biomedical Analysis Medical SchoolTsinghua UniversityBeijing100084China Key Laboratory of Cardiovascular Remodeling and Function Research Department of CardiologyQilu Hospital of Shandong UniversityJinan250012China Department of Biomedical Engineering College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan430074China State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University–Peking University Joint Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing100084China

Autophagy is a lysosome-based degradation pathway,characterized by formation of a double-membrane vesicle named the *** autophagy,various cargos are engulfed and delivered to lysosomes(or the vacuole in yeast)for *** is an evolutionarily conserved pathway and plays important roles in various physiological and pathological conditions such as develop-ment,immunity,cancer and neuronal degeneration(Jiang and Mizushima,2014;Martin et al.,2014).

关键词：蛋白磷酸化自噬位点分析降解途径溶酶体双层膜液泡进化

Neuroprotective effects of cromakalim on cerebral ischemia-reperfusion injury in rats Correlation with hippocampal metabotropic glutamate receptor 1 alpha and glutamate transporter 1

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Neural Regeneration Research 2010年第9期5卷 678-682页

作者： Shilei Wang Junchao liu Qingxian Chang yu Li, Yan Jiang Shiduan Wang Department of Anesthesiology Affiliated Hospital of Qingdao University Medical CollegeQingdao 266003Shandong ProvinceChina

BACKGROUND：Studies have reported that potassium channel openers exhibit a protective effect on cerebral ischemia-reperfusion injury and inhibit glutamate excitotoxicity in ***,the effects of the glutamate receptor 1α and glutamate transporter 1 remain poorly ***：To investigate the prophylactic use of the adenosine triphosphate-sensitive potassium channel opener cromakalim on neurological function and cerebral infarct size,as well as glutamate receptor 1α and glutamate transporter 1 expression,in rats with cerebral ischemia-reperfusion injury,and to explore action mechanisms underlying reduced glutamate excitotoxicity and neuroprotection in ***,TIME AND SETTING：Randomized,controlled,animal experiment was performed at the Brain Institute,Qingdao University Medical College,Between July 2008 and April ***：Cromakalim was purchased from Sigma,USA; rabbit anti-glutamate receptor 1α polyclonal antibody was offered by Wuhan Boster,China; rabbit anti-glutamate transporter 1 polyclonal antibody was offered by Santa Cruz Biotechnology,***：Sixty male,Wistar rats,aged 6 months,were randomly assigned to three groups （n =20）：sham-surgery,model,and *** thread methods were used to establish middle cerebral artery occlusion in rats from the model and cromakalim *** from the sham-surgery group were subjected to exposed common carotid artery,external carotid artery,and internal carotid artery,without *** （10 mg/kg） was administered 30 minutes prior to middle cerebral artery occlusion,but there was no intervention in the model and sham-surgery *** OUTCOME MEASURES：At 24 hours post-surgery,neurological behavioral functions were evaluated using Bederson＇s test,cerebral infarction volume was determined following tetrazolium chloride staining,and glutamate receptor 1a and glutamate transporter 1 expressions were detected using ***：Following cerebral ischemia-repe

关键词： cerebral ischemia-reperfusion cromakalim glutamate receptor 1α glutamate transporter 1

Prognostic value of c-Met in colorectal cancer:A metaanalysis

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World Journal of Gastroenterology 2015年第12期21卷 3706-3710页

作者： Yan liu Xiao-Feng yu Jian Zou Zi-Hua Luo Department of Gastroenterology Huadong Hospital Fudan University

AIM: To assess the prognostic value of c-Met status in colorectal cancer. METHODS: We conducted a search in Pub Med, Web of Science, and the Cochrane Library covering all published papers up to July 2014. Only studies assessing survival in colorectal cancer by c-Met status were included. This meta-analysis was performed by using ***: Ultimately, 11 studies were included in this analysis. Meta-analysis of the hazard ratios(HR)indicated that patients with high c-Met expression have a significantly poorer overall survival(OR)(HR = 1.33, 95%CI: 1.06-1.59) and progression-free survival(PFS)(HR = 1.47, 95%CI: 1.03-1.91). Subgroup analysis showed a significant association between high c-Met expression and poorer overall survival in the hazard ratio reported(HR = 1.41, 95%CI: 1.08-1.74).CONCLUSION: The present meta-analysis indicated that high c-Met expression was associated with poor prognosis in patients with colorectal cancer.

关键词： Colorectal cancer Prognosis c-Met Metaanalysis Ove

Description of a Marine Nematode Hopperia sinensis sp. nov.(Comesomatidae) from Mangrove Forests of Quanzhou, China, with a Pictorial Key to Hopperia Species

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Journal of Ocean University of China 2015年第6期14卷 1111-1115页

作者： GUO yuqing CHANG yu CHEN yuzhen LI Yongxiang liu Aiyuan Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment Fisheries CollegeJimei University Library Jimei University

A new free-living marine nematode species Hopperia sinensis sp. nov. from mangrove forests of Fujian Province, China, is identified and illustrated. Hopperia sinensis sp. nov. is characterized by its cephalic setae 2.4–2.8 μm long or 17%–20% head diameter, and amphids of 2.25–2.5 turns. Lateral differentiation appears with larger, more irregularly distributed dots behind 3–5 transverse rows of dots posterior to amphid. Buccal cavity is consisted of a shallow and weakly sclerotized cup-shaped portion with strongly sclerotized walls of 18–21 μm deep. There are three sclerotized and size-equally pointed teeth at the junction between the two parts. Spicules of 41–45 μm long are slightly curved with broadband velum and central strips at the proximal end. The gubernacula, with apparent lateral guiding pieces, are formed by one central tubular piece that is weakly sclerotized with 11–16 μm long dorso-caudally directed apophyses. There are 13–14 fine tubular precloacal supplements. Conico-cylindrical tail gradually tapers till pointed tail tip. Female is similar to male, but have a longer body and tail. Ovaries are opposed and outstretched, with anterior ovary to the left and posterior ovary to the right of the intestine. A pictorial key to all the valid known species in genus Hopperia is given.

关键词： free-living marine nematode new species Hopperia East China Sea mangrove

From molecular dynamics to lattice Boltzmann:a new approach for pore-scale modeling of multi-phase flow

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Petroleum Science 2015年第2期12卷 282-292页

作者： Xuan liu Yong-Feng Zhu Bin Gong Jia-Peng yu Shi-Ti Cui College of Engineering Peking University Sinopec Petroleum Exploration and Production Research Institute Petrochina Tarim Oilfield Exploration and Production Research Institute

Most current lattice Boltzmann （LBM） models suffer from the deficiency that their parameters have to be obtained by fitting experimental results. In this paper, we propose a new method that integrates the molecular dynamics （MD） simulation and LBM to avoid such defect. The basic idea is to first construct a molecular model based on the actual components of the rock-fluid system, then to compute the interaction force between the rock and the fluid of different densities through the MD simulation. This calculated rock-fluid interaction force, combined with the fluid-fluid force determined from the equation of state, is then used in LBM modeling. Without parameter fitting, this study presents a new systematic approach for pore-scale modeling of multi-phase flow. We have validated this ap- proach by simulating a two-phase separation process and gas-liquid-solid three-phase contact angle. Based on an actual X-ray CT image of a reservoir core, we applied our workflow to calculate the absolute permeability of the core, vapor-liquid H20 relative permeability, and capillary pressure curves.

关键词： Molecular dynamics - Lattice BoltzmannMulti-phase flow Core simulation

Structure and Antibacterial Activity of Modified Montmorillonite

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Chemical Research in Chinese Universities 2010年第4期26卷 509-513页

作者： XIE yu-shan TAN Shao-zao LIAO Ma-hua liu Ren-fu Department of Chemistry Jinan University Guangzhou 510632 P. R. China

Tetradecyldimethylbenzyl ammonium salt（TDMBA） cations were intercalated into sodium montmorillonite（Na-MMT） by an ion exchange reaction. The resulting TDMBA-MMT was characterized by Fourier-transform infrared spectroscopy（FTIR）, thermogravimetric analysis（TGA）, and X-ray diffraction（XRD）. Moreover, the antibacterial activity and water-resistance of TDMBA-MMT were investigated. The results show that TDMBA cations are intercalated into the Na-MMT. The onset temperature of decomposition of TDMBA cations in TDMBA-MMT is raised about 60 ℃ higher than that of tetradecyldimethylbenzyl ammonium chloride（TDMBACl）, and the d（001） spacing enlarged from 1.23 nm to 2.10 nm. 0.1 mg/mL TDMBA-MMT is fully enough to kill all the Staphylococci aureus（S, aureus） and more than 99.99% of the Escherichia coli（E, coli） in a sample solution within 6.0 h touch, and the TDMBA-MMT also shows long acting properties.

关键词： Na-MMT TDMBA cation Surface modification Structure Antibacterial activity

DNA barcoding and rapid identification of the precious herb Herba Anoectochili

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Chinese Journal of Natural Medicines 2019年第10期17卷 738-745页

作者： HU Shuai-Jun HU Hao-yu GAO Han liu Xia CHEN Shi-Lin School of Chemistry Chemical Engineering and Life SciencesWuhan University of TechnologyWuhan 430070China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China

Herba Anoectochili is a commonly used medicinal material. However, its adulteration is a serious concern. Due to the similar morphological characteristics of Herba Anoectochili and its adulterants, traditional identification techniques often fail to distinguish between them accurately, which is not conducive to the circulation management and safety of the medicinal materials. To improve the distinction between Herba Anoectochili and its adulterants accurately, this study identified 41 Herba Anoectochili and its adulterant samples based on the ITS2 sequence. Sequence characteristics, Basic Local Alignment Search Tool(BLAST) application, genetic distance, construction of phylogenetic tree, secondary structure prediction, and other methods showed the ITS2 sequence to accurately identify Herba Anoectochili from its adulterants. Furthermore, in this study, we designed a specific primer, based on the ITS2 sequence, and established a real-time PCR detection system for the rapid, sensitive, and specific identification of the original plant of Herba Anoectochili. Compared to DNA barcoding technology, this method has shorter detection time, stronger specificity, and higher sensitivity, which lays the foundation for the rapid identification of Herba Anoectochili.

关键词： Herba Anoectochili ITS2 DNA barcode Real-time PCR Rapid identification

Amplitudes, rates, periodicities and causes of temperature variations in the past 2485 years and future trends over the central-eastern Tibetan Plateau

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Chinese Science Bulletin 2011年第28期56卷 2986-2994页

作者： liu yu CAI QiuFang SONG HuiMing AN ZhiSheng Hans W. LINDERHOLM The State Key Laboratory of Loess and Quaternary Geology Institute of Earth Environment Chinese Academy of Sciences Xi＇an 710075 China Department of Environmental Science and Technology School of Human Settlements and Civil Engineering Xi＇an Jiaotong University Xi＇an 710049 China Graduate University of Chinese Academy of Sciences Beijing 100049 Chin Regional Climate Group Department of Earth Sciences University of Gothenburg SE-40530 Gothenburg Sweden

Amplitudes, rates, periodicities, causes and future trends of temperature variations based on tree rings for the past 2485 years on the central-eastern Tibetan Plateau were analyzed. The results showed that extreme climatic events on the Plateau, such as the Medieval Warm Period, Little Ice Age and 20th Century Warming appeared synchronously with those in other places worldwide. The largest amplitude and rate of temperature change occurred during the Eastern Jin Event (343-425 AD), and not in the late 20th century. There were significant cycles of 1324 a, 800 a, 199 a, 110 a and 2-3 a in the 2485-year temperature series. The 1324 a, 800 a, 199 a and 110 a cycles are associated with solar activity, which greatly affects the Earth surface temperature. The long-term trends (>1000 a) of temperature were controlled by the millennium-scale cycle, and amplitudes were dominated by multi-century cycles. Moreover, cold intervals corresponded to sunspot minimums. The prediction indicated that the temperature will decrease in the future until to 2068 AD and then increase again.

关键词：青藏高原东部温度变化发展趋势周期性幅度速率原因中央

Experimental investigation of p-section concrete beams under contact explosion and close-in explosion conditions

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Defence Technology（防务技术） 2018年第5期14卷 540-549页

作者： Zhen Li Yan liu Jun-bo Yan Wen-li yu Feng-lei Huang State Key Laboratory of Explosion Science and Technology Beijing Institute of Technology Department of Nuclear Engineering Xi'an Research Institute of High-Tech

This paper presents the results of an experimental investigation on explosive breaching of p-section concrete beams. Twenty three p-section concrete beams with a 100 cm length were tested. TNT charges were placed at three positions: contact detonation in the center, contact detonation above the web and close-in detonation in the center. The external and internal breach parameters of the panels were evaluated by measuring the diameter of the ejection crater, spalling crater and breach hole created by the charge detonation. The experimental results were compared to predict values obtained by the analytical models proposed by McVay, Morishita and Remennikov. A modified breach with crater limit line and breach without crater limit line were put forward based on the experimental results. The maximum cross-sectional destruction area ratio(MCDAR) values were used to evaluate the damage degree. The maximum value of MCDAR reached 0.331 corresponding to the C5 experimental condition, of which explosion occurred above the web.

关键词： P-section concrete beam Contact explosion Close-in explosion Explosive breach