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2014年全国桑树病虫防控学术研讨会

作者： Fanwei Dai Guoqing Luo Zhenjiang Wang cuiming tang Sericulture ＆ Agri-Food Research Institut Guangdong Academy of Agricultural Sciences

Bacterial wilt caused by Ralstonia solanacearum is a major disease causes severe yield and quality losses in mulberry（Morusatropurpurea）,but little is known about the molecular mechanism of *** RNA-seq technique,we analyzed transcriptional changes of resistance（KQ10 and YS283） and susceptible（YSD10）mulberry cultivars at the early interaction in response to *** *** observed 798 genes were regulated specifically in both resistant cultivars but not in susceptible cultivar after infection with ***,including 502 up-regulated and 296 down-regulated genes,***,we found a large amount of genes（61 genes） associated with cell wall modification were regulated specifically in resistant cultivars,which were divided into ten major *** largest group was glucosyltransferase family,following pectinesterase inhibitor,glucanase,and glycoprotein families,suggesting that cell wall modifications play a major role on resistance levels in *** transcriptional analysis paves the way forelucidating the molecular mechanisms in the resistance response to *** in mulberry.

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Phenolic Profiles and Antioxidant Capacities of Mulberry（Morus atropurpurea Roxb.） Fruit of Different Cultivars

Phenolic Profiles and Antioxidant Capacities of Mulberry（Mo...

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全国桑树产业发展学术研讨会

作者： Zhenjiang Wang cuiming tang Fanwei Dai Gengsheng Xiao Guoqing Luo Sericultural ＆ Agri-Food Research Institute Guangdong Academy of Agricultural Sciences

The total phenolic contents and antioxidant capacities of 274 mulberry fruit samples from fourprovinces in China were 412.55–1471.86 mg/L gallic acid equivalent and 37.90–361.75 mmol/mLferrous sulfate equivalent,respectively.A cluster analysis separated the cultivars into twogroups;One consisting of samples from Guangdong,Guangxi and Hainan Provinces,which hadhigh phenolic contents and antioxidant capacities,and the other consisting ofsamples from Xinjiang Province,which hadlow phenolic contents and antioxidant *** activity and phenolic content were correlated.A high-performance liquid chromatography with diode-array detection quadrupole time-of-flight tandem mass spectrometry analysis was established for the identification of therepresentative mulberry fruit cultivar Da10 to identify the major *** the main polyphenols of ’Da10’.Additionally,other kinds of bound phenolic compounds,notjust thefree forms,were *** general,’45-7’,’qianshan3’ and ’guoxuan04-129’ were superiorcultivars based on theirhigh phenolic contents and antioxidant capacities.

关键词： mulberry fruit polyphenols antioxidant capacity HPLC-DAD-Q-TOF-MS/MS

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Genetic diversity among mulberry genotypes from seven countries

Genetic diversity among mulberry genotypes from seven countr...

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中国蚕学会第九届青年学术研讨会

作者： Zhenjiang Wang Fanwei Dai Guoqing Luo Gengsheng Xiao cuiming tang Sericultural ＆ Agri-Food Research Institute Guangdong Academy of Agricultural Sciences

Mulberry（Morus alba） is an economically and ecologically important,widespread woody *** has served human beings for over hundreds of years,and it is still widely used in pharmaceuticals,food industry and farming *** modern techniques,deeper understanding in classification and conservation resources of mulberry leads to higher-efficiency hybrids among *** polymorphisms among 42 mulberry genotypes from seven countries over Asia and South America were detected using 17 inter simple sequence repeat（ISSR） primers.A dendrogram was constructed using the similarity matrix among genotypes and a principal component analysis（PCA） was carried out to further identify and cluster the mulberry *** the 42 genotypes,175 distinct bands were displayed,among which 169 were polymorphic bands（96.57%）.The polymorphic information content of 17 ISSR primers ranged from 0.2921 to 0.3746 with the mean of *** Nei‘s index and Shanon‘s information index averaged 0.116 and 0.174,respectively,indicating low diversity of *** further study,cluster analysis and PCA were carried out and the results were similar.42 genotypes were grouped,showing some hybridized ***,a connection between mulberry diseases and their genotypes was noted,which indicates possible application for ISSR in studying disease resistance of mulberry.

关键词： Genetic diversity Inter simple sequence repeat Mulberry Cluster analysis Principal component analysis

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HPLC determination of phenolic compounds in different solvent extracts of mulberry leaves and antioxidant capacity of extracts

HPLC determination of phenolic compounds in different solven...

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中国蚕学会2018年学术年会

作者： Zhenjiang Wang Fanwei Dai Gengsheng Xiao Guoqing Luo cuiming tang Sericulture ＆ Agri-Food Research Institute Guangdong Academy of Agricultural Sciences Key Laboratory of Urban Agriculture in South China Ministry of Agriculture

The antioxidant properties and phenolic content of extracts of mulberry leaves obtained using aqueous mixtures of ethanol, methanol and acetone were determined. Both alkaline hydrolysis and acid hydrolysis were used to extract bound *** was used todeterminethe profile of the extracted phenolics. Threeexperimental assays, Fe3+reducing antioxidant power（FRAP）, ABTS and DPPH radical scavenging activity,were used to characterizetheantioxidant activity of the extracts. Acetone proved to be the most efficient solvent for extraction of free phenolics from mulberry leaves and the acetone extract had the highest antioxidant capacity. Alkaline hydrolysis was found to bethe best method for extracting bound phenolics and provided an extract with better antioxidant properties than acid hydrolysis.

关键词： Mulberry leaves phenolic compounds solvent extraction antioxidant activity HPLC

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Phenolic content and antioxidant activity of twenty three representative mulberry leaf varieties grown indifferent regions and countries

Phenolic content and antioxidant activity of twenty three re...

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中国蚕学会2018年学术年会

作者： Zhenjiang Wang Fanwei Dai Guoqing Luo Gengsheng Xiao cuiming tang Sericulture ＆ Agri-Food Research Institute Guangdong Academy of Agricultural Sciences Key Laboratory of Urban Agriculture in South China Ministry of Agriculture

Twenty three major mulberry leaves（Gu 1,Gu 6,Gu 8, Gusangwang of North China;Bei-2-8, R 2,7403, Dashi of South China;Zangjiangxin 1, Zangjiangxin 2, Zangjiangxin 4 of Southwest China;Changguosang of Taiwan;JP4-1, JP5 of Japan;TL6, TL7, BR60, S54, Qingmai of Thailand;Yin 2, Yinximeng 2 of India and Yue 1, Yueda of Vietnam）cultivated in aforementioned regions and countries were assessed for their polyphenolic composition using HPLC, and tested for antioxidant potential by three different assays（FRAP, ABTS ＆ DPPH）. The results showed that Changguosang from Taiwan had the highest quantity of phenol and also became the strongest antioxidant capacity among the twenty three cultivars which was agreed by FRAP, ABTS and DPPH *** chlorogenic acid wasthe predominate one in free phenol content and caffeic acid was the major in bound ***, chlorogenic acid, syringic acid and rutin mainly contributed to the antioxidant capacity of free phenol while syringic acid also was positively correlated with the antioxidant of bound phenol.

关键词： phenolic compounds chlorogenic acid rutin caffeic acid HPLC

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Phenolic Profiles and Antioxidant Capacities of Mulberry（Morus atropurpurea Roxb.） Fruit of Different Cultivars

Phenolic Profiles and Antioxidant Capacities of Mulberry（Mo...

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中国蚕学会第九届青年学术研讨会

作者： Zhenjiang Wang cuiming tang Fanwei Dai Gengsheng Xiao Guoqing Luo Sericultural ＆ Agri-Food Research Institute Guangdong Academy of Agricultural Sciences

关键词： mulberry fruit·polyphenols·antioxidant capacity·HPLC-DAD-Q-TOF-MS/MS

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Proteomic and phosphoproteome analysis of mulberry leaves response to salt-stress

Proteomic and phosphoproteome analysis of mulberry leaves re...

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第十九届中国作物学会学术年会

作者： Wang Yi Dai Fanwei Wang Zhenjiang Luo Guoqing tang cuiming Sericulture & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences Key Laboratory of Urban Agriculture in South China Ministry of Agriculture and Rural Affairs

Salinity is one of the most common abiotic stresses encountered by plants. Reversible protein phosphorylation is involved in plant defense processes against salinity stress. Here, the different physiological responses were studied by comparing their tolerances to 200 m M/L Na Cl concentrations in mulberry for 0, 5 and 10 *** performed global phosphopeptide mapping through enrichment by our synthesized PVA-phosphate-Ti4+ IMAC coupled with subsequent identification by ESI-Q-TOF MS. A total of 104 peptide sequences containing 139 phosphorylation sites were determined from 70 phosphoproteins of the control leaves. In contrast, 124 phosphopeptides containing 143 phosphorylated sites from 92 phosphoproteins were identified in salt-stressed maize leaves. Compared with the control, 47 proteins were phosphorylated, 25 were dephosphorylated, and 45 overlapped. Among the 72 differential phosphoproteins, 35 were known salt stress response proteins and the rest had not been reported in the literature. To dissect the differential phosphorylation, gene ontology annotations were retrieved for the differential phosphoproteins. The results revealed that cell signaling pathway members such as calmodulin and 14-3-3 proteins were regulated in response to 24-h salt stress. Multiple putative salt-responsive phosphoproteins seem to be involved in the regulation of photosynthesis-related processes. These results may help to understand the salt-inducible phosphorylation processes of maize leaves.

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Genetic diversity among mulberry genotypes from seven countries

Genetic diversity among mulberry genotypes from seven countr...

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全国桑树产业发展学术研讨会

作者： Zhenjiang Wang Fanwei Dai Guoqing Luo Gengsheng Xiao cuiming tang Sericultural ＆ Agri-Food Research Institute Guangdong Academy of Agricultural Sciences

Mulberry（Morus alba） is an economically and ecologically important,widespread woody *** has served human beings for over hundreds of years,and it is still widely used in pharmaceuticals,food industry and farming *** modern techniques,deeper understanding in classification and conservation resources of mulberry leads to higher-efficiency hybrids among *** polymorphisms among 42 mulberry genotypes from seven countries over Asia and South America were detected using 17 inter simple sequence repeat（ISSR） primers.A dendrogram was constructed using the similarity matrix among genotypes and a principal component analysis（PCA） was carried out to further identify and cluster the mulberry *** the 42 genotypes,175 distinct bands were displayed,among which 169 were polymorphic bands（96.57%）.The polymorphic information content of 17 ISSR primers ranged from 0.2921 to 0.3746 with the mean of *** Nei’s index and Shanon’s information index averaged 0.116 and 0.174,respectively,indicating low diversity of *** further study,cluster analysis and PCA were carried out and the results were similar.42 genotypes were grouped,showing some hybridized ***,a connection between mulberry diseases and their genotypes was noted,which indicates possible application for ISSR in studying disease resistance of mulberry.

关键词： Genetic diversity Inter simple sequence repeat Mulberry Cluster analysis Principal component analysis

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De novo assembly, gene annotation and marker development of mulberry（Morus atropurpurea） transcriptome

De novo assembly, gene annotation and marker development of ...

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中国蚕学会第八届青年学术研讨会

作者： Fanwei Dai cuiming tang Zhenjiang Wang Guoqing Luo Li He Liuhui Yao The Sericulture and Agri-Food Research Institute Guangdong Academy of Agricultural Sciences

Mulberry（Morus atropurpurea） is an economically important tree with a long history of extensive cultivation in Asia because it is the exclusive food source for the silkworm（Bombyx mori）. Recently, mulberry has gained additional commercial value as a source for medicinal compounds, as animal fodder, and as landscaping trees. In the present work, the mulberry transcriptome was sequenced using Illumina paired-end sequencing technology. A total of 105 million 90-bp paired-end reads were generated, and60,069 unigenes were assembled with an N50 of 1,219 bp. Based on a sequence similarity search with known proteins, 40,121 genes were identified. Among these genes, 31,548 were annotated with 55 gene ontology functional categories, 7,790 had a Cluster of Orthologous Groups classification, and 23,188 mapped to 128 biological pathways in the Kyoto Encyclopedia of Genes and Genomes pathway database. In addition, 10,268 microsatellites were developed and characterized as potential molecular *** data will accelerate the understanding of mulberry growth and development mechanisms and facilitate gene discovery and functional genomic studies in mulberry.

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基于文献计量分析的根系分泌物研究进展

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中国农学通报 2024年第7期40卷 144-154页

作者：王雨菡陈莲张培珍李高聪王振江唐翠明林森罗国庆钟建武李智毅王圆广东省农业科学院蚕业与农产品加工研究所广州510610 广东海洋大学电子与信息工程学院广东湛江524088 农业农村部华南都市农业重点实验室广州510610

为深入了解根系分泌物研究进展及发展趋势,以Web of Science核心合集数据库2002-2022年根系分泌物的相关文献为数据源,采用文献计量学方法,借助软件VOSviewer和CiteSpace对年发文量、高产发文国家间合作关系、发文机构、期刊分布、文献... 详细信息

为深入了解根系分泌物研究进展及发展趋势,以Web of Science核心合集数据库2002-2022年根系分泌物的相关文献为数据源,采用文献计量学方法,借助软件VOSviewer和CiteSpace对年发文量、高产发文国家间合作关系、发文机构、期刊分布、文献共被引和高频关键词等进行可视化处理,并对不同研究阶段主要研究内容进行分析。结果表明,近20年根系分泌物研究领域年发文量总体呈上升趋势,在国际范围内受重视程度越来越高。中国、美国合作关系密切且合作发文最多,中国科学院与美国科罗拉州立大学致力于该领域研究并做出突出贡献,《Plant and Soil》和《Soil Biology&Biochemistry》为主要载体。生态系统、根际微生物、有机酸、基因、胁迫和植物适应性为根系分泌物研究的核心主题;不同研究阶段主要研究内容由早及近表现为信号传导—养分获取—化感作用—植物修复—植物与微生物互作。在分子水平上揭示植物与根际微生物的互作机理、根系分泌物与根际微生物在植物生长过程中的调控功能及分子机制等更为细化的研究将会成为未来一段时间的新兴研究热点。

关键词：根系分泌物可视化分析文献计量 VOSviewer CiteSpace

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