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Ultrasensitive label-free electrochemiluminescence immunosensor based on N-(4-aminobutyl)-N-ethylisoluminol-functionalized graphene composite

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Science China Chemistry 2015年第3期58卷 425-432页

作者： Jiangnan Shu Wen Shen hua cui CAS Key Laboratory of Soft Matter Chemistry Department of Chemistry University of Science and Technology of China

The electrochemiluminescence(ECL) behavior of N-(4-aminobutyl)-N-ethylisoluminol(ABEI)-functionalized graphene composite(ABEI-GC) modified on an indium tin oxide(ITO) electrode was studied. ABEI-GC exhibited excellent ECL activity. On this basis, a label-free ECL immunosensor was developed for the sensitive detection of human immunoglobulin G(h Ig G) by using ABEI-GC as the ECL nano-interface via a layer-by-layer assembly technique. ABEI-GC was first assembled onto an ITO electrode. Positively charged chitosan was then electrostatically adsorbed to the modified electrode. Finally, negatively charged antibody-coated gold nanoparticles were attached to the surface to form the ECL immunosensor. In the presence of h Ig G, h Ig G was captured by its antibody. In addition, an ECL signal was detected in the presence of H2O2 when a double potential was applied. The ECL immunosensor for the determination of h Ig G showed a linear range of 1.0×10-13–1.0×10-8 g/mL with a detection limit of 5.0×10-14 g/m L. This immunosensor has high sensitivity, wide linearity and good reproducibility. The superior sensitivity of the proposed ECL immunoassay mainly derives from the incorporation of ABEI-GC, which not only improves the ECL intensity, response speed, and stability, but also provides a large specific surface for high levels of protein loading. This work reveals that ABEI-GC is good nano-interface for the construction of ECL biosensors. Our strategy is promising for protein detection and may open up a new avenue for ultrasensitive label-free immunoassays.

关键词： N-(aminobutyl)-N-(ethylisoluminol)(ABEI) graphene electrochemiluminescence immunosensor

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Highly chemiluminescent magnetic mesoporous carbon composites Fe_3O_4@void@C with yolk-shell structure

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Science China Chemistry 2018年第9期61卷 1143-1150页

作者： Rui Yang Shaomin Liu hua cui CAS Key Laboratory of Soft Matter Chemistry Collaborative Innovation Center of Chemistry for Energy Materials (iChEM)Department of Chemistry University of Science and Technology of China

In this work,highly chemiluminescent magnetic mesoporous carbon with yolk-shell structure was synthesized by encapsulating N-(4-aminobutyl)-N-ethylisoluminol(ABEI)and Co^(2+)into the magnetic mesoporous carbon composites(Co^(2+)-ABEI-Fe_3O_4@void@C).The synthetic Co^(2+)-ABEI-Fe_3O_4@void@C showed a good magnetic separation property,which could remove residual ABEI molecules and Co^(2+)in less than 3 min under an external ***,the synthetic Co^(2+)-ABEI-Fe_3O_4@void@C demonstrated good chemiluminescence(CL)property and good stability when interacted with alkaline H_2O_*** CL intensity of such Co^(2+)-ABEI-Fe_3O_4@void@C was about 120 times higher than that of ABEI-Fe_3O_4@void@*** Co^(2+)-ABEI-Fe_3O_4@void@C also exhibited good electrochemiluminescence(ECL)property in alkaline *** outstanding CL/ECL performance of the Co^(2+)-ABEI-Fe_3O_4@void@C was attributed to the Co^(2+)immobilized in the Co^(2+)-ABEI-Fe_3O_4@void@C,which catalyzed the decomposition of H_2O_2to generate O_2^(·-)and HO~·,expediting the CL/ECL *** synthetic Co^(2+)-ABEI-Fe_3O_4@void@C may be of great application for the development of new methodologies in bioanalysis.

关键词： N-(4-aminobutyl)-N-ethylisoluminol(ABEI) chemiluminescence(CL) yolk-shell magnetic mesoporous carbon

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Preparation of Chlorinated Poly(propylene carbonate) and Its Distinguished Properties

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Chinese Journal of Polymer Science 2017年第9期35卷 1086-1096页

作者： Xi-hua cui Jing Jin Jie cui 赵桂艳姜伟 State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China University of Science and Technology of China Hefei 230026 China

Poly（propylene carbonate）（PPC）, the copolymerization product of carbon dioxide and propylene oxide, was chlorinated for the first time in our laboratory. Nuclear magnetic resonance （NMR） spectroscopy and ion chromatography test showed that chlorine atoms were successfully introduced onto the polymer chains of PPC. We named this newborn polymer material as chlorinated poly（propylene carbonate）（CPPC）. It is worth noting that the reaction conditions of the chlorination of PPC were quite mild, which could be easily and simply realized at industrial level. What is more important is that CPPC possessed many more distinguished properties in solubility, wettability, adhesiveness, and gas barrier compared with PPC. For example, the bonding strength of CPPC as thermal adhesive is nearly four times higher than that of PPC for wood, stainless steel and glass. The oxygen permeability coefficient of CPPC exhibits a decrease of 33% compared with that of PPC. Moreover, CPPC is quite stable in air, whereas it could be well biodegraded in soil compared with PPC. These results indicated that CPPC could be widely used in the fields of coating, adhesive, barrier materials and so on, which could greatly promote the development of PPC industry.

关键词： Poly(propylene carbonate) Chlorination Biodegradability Adhesive Gas barrier

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Microstructure evolution and phase transformation of traditional cast and spray-formed hypereutectic aluminium-silicon alloys induced by heat treatment

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International Journal of Minerals,Metallurgy and Materials 2010年第3期17卷 297-306页

作者： Long-gang Hou Yuan-hua Cai hua cui Ji-shan Zhang State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing Beijing 100083 China School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China

Microstructural evolution and phase transformation induced by different heat treatments of the hypereutectic aluminium-silicon alloy, Al-25Si-5Fe-3Cu （wt%, signed as 3C）, fabricated by traditional cast （TC） and spray forming （SF） processes, were investigated by differential scanning calorimetry （DSC） and scanning electron microscopy （SEM） combined with energy dispersive X-ray spectroscopy and X-ray diffraction techniques. The results show that A17Cu2Fe phase can be formed and transformed in TC- and SF-3C alloys between 802-813 K and 800-815 K, respectively. The transformation from β-Al5FeSi to δ-Al4FeSi2 phase via peritectic reaction can occur at around 858-870 K and 876-890 K in TC- and SF-3C alloys, respectively. The starting precipitation temperature of δ-Al4FeSi2 phase as the dominant Fe-bearing phase in the TC-3C alloy is 997 K and the exothermic peak about the peritectic transformation of δ-Al4FeSi2→β-Al5FeSi is not detected in the present DSC experiments. Also, the mechanisms of the microstructural evolution and phase transformation are discussed.

关键词： hypereutectic Al-Si alloy spray forming cast microstructure phase transformation

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Innate immunity in tuberculosis: host defense vs pathogen evasion

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Cellular & Molecular Immunology 2017年第12期14卷 963-975页

作者： cui hua Liu Haiying Liu Baoxue Ge CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Savaid Medical School University of Chinese Academy of SciencesBeijing 101408China MOH Key Laboratory of Systems Biology of Pathogens Institute of Pathogen Biologyand Center for Tuberculosis ResearchChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100176China Shanghai Key Lab of Tuberculosis Shanghai Pulmonary HospitalTongji University School of MedicineShanghai 200433China

The major innate immune cell types involved in tuberculosis(TB)infection are macrophages,dendritic cells(DCs),neutrophils and natural killer(NK)*** immune cells recognize the TB-causing pathogen Mycobacterium tuberculosis(Mtb)through various pattern recognition receptors(PRRs),including but not limited to Toll-like receptors(TLRs),Nod-like receptors(NLRs)and C-type lectin receptors(CLRs).Upon infection by Mtb,the host orchestrates multiple signaling cascades via the PRRs to launch a variety of innate immune defense functions such as phagocytosis,autophagy,apoptosis and inflammasome *** contrast,Mtb utilizes numerous exquisite strategies to evade or circumvent host innate *** we discuss recent research on major host innate immune cells,PRR signaling,and the cellular functions involved in Mtb infection,with a specific focus on the host’s innate immune defense and Mtb immune evasion.A better understanding of the molecular mechanisms underlying host–pathogen interactions could provide a rational basis for the development of effective anti-TB therapeutics.

关键词： host–pathogen interactions immune evasion innate immune defense Mycobacterium tuberculosis tuberculosis

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Design and Synthesis of Chiral Molecular Tweezers Based on Deoxycholic Acid

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Chinese Chemical Letters 2001年第5期12卷 413-416页

作者： cui hua XUE Qi Ming MU Shu hua CHEN The Faculty of chemistry Sichuan UniversityChengdu 610064

A series of new chiral molecular tweezers have been designed and synthesized by using deoxycholic acid as spacer and aromatic amines as arms. Instead of using toxic phosgene, the triphosgene was employed in synthesis of the molecular tweezers receptors. These chiral molecular tweezers showed good enantioselectivity for D-amino acid methyl esters.

关键词： Molecular tweezers synthesis deoxycholic acid recognition.

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Electronic structure and properties of FeS_2 with the space groups of Pa3 and P1

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International Journal of Minerals,Metallurgy and Materials 2013年第7期20卷 671-677页

作者： cui-hua Zhao Bo-zeng Wu Jian-hua Chen Yu-qiong Li Ye Chen School of Chemistry and Chemical Engineering Guangxi University China Tin Group Co. Ltd. College of Material Science and Engineering Guangxi University College of Resources and Metallurgy Guangxi University

The electronic structure and properties of FeS2 with the space groups of Pa3 and P1 were studied by the density functional theory. The generalized-gradient approximation exchange-correlation functional was used in conjunction with a plane wave-ultrasoft pseudopotential representation. Calculation results show that differences are observed in electronic structures and properties between Pa3 and P1 crystals. The band gap and energy loss of P1 are smaller than those of Pa3 crystal, while the dielectric constant, conductivity, refractive index, extinction coefficient, and intensity of optical absorption of P1 are larger than those of Pa3. These behaviors are attributed to the differences in symmetry, atomic arrangement, and Mulliken bond population of each unit for Pa3 and P1 crystals.

关键词： pyrites electronic structure optical properties crystal structure density functional theory

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The ubiquitin system： a critical regulator of innate immunity and pathogen-host interactions

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Cellular & Molecular Immunology 2016年第5期13卷 560-576页

作者： Jie Li Qi-Yao Chai cui hua Liu CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China

The ubiquitin system comprises enzymes that are responsible for ubiquitination and deubiquitination, as well as ubiquitin receptors that are capable of recognizing and deciphering the ubiquitin code, which act in coordination to regulate almost all host cellular processes, including host-pathogen interactions. In response to pathogen infection, the host innate immune system launches an array of distinct antimicrobial activities encompassing inflammatory signaling, phagosomal maturation, autophagy and apoptosis, all of which are fine-tuned by the ubiquitin system to eradicate the invading pathogens and to reduce concomitant host damage. By contrast, pathogens have evolved a cohort of exquisite strategies to evade host innate immunity by usurping the ubiquitin system for their own benefits. Here, we present recent advances regarding the ubiquitin system-mediated modulation of host-pathogen interplay, with a specific focus on host innate immune defenses and bacterial pathogen immune evasion.

关键词： apoptosis autophagy bacterial pathogen innate immune signaling phagosomal maturation ubiquitin system

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Pt and Te codoped ultrathin MoS_(2) nanosheets for enhanced hydrogen evolution reaction with wide pH range

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Rare Metals 2022年第2期41卷 378-384页

作者： cui-hua an Wei Kang Qi-Bo Deng Ning Hu National Engineering Research Center for Technological Innovation Method and Tool School of Mechanical EngineeringHebei University of TechnologyTianjin 300401China Key Laboratory of Advanced Energy Materials Chemistry(MOE) College of ChemistryNankai UniversityTianjin 300071China Institute for New Energy Materials&Low-Carbon Technologies School of Materials Science and EngineeringTianjin University of TechnologyTianjin 300384China State Key Laboratory of Reliability and Intelligence Electrical Equipment Hebei University of TechnologyTianjin 300130China

Transition metal dichalcogenides,especially MoS_(2),have been examined as promising catalysts for hydrogen evolution reaction(HER).Meanwhile,MoS_(2)with various nanostructures and exposed more active sites has been broadly explored for boosting the HER performances at wide pH ***,a synergistic Pt and Te codoping strategy has been used to enhance the catalytic performance(including reducing the overpotential and promoting the HER reaction kinetics)of ultrathin MoS_(2)nanosheets in alkaline and acid electrolytes,which has delivered better than single Pt doping or single Te doping.

关键词： doping kinetics synergistic

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Rolling motion behavior of rockfall on gentle slope:an experimental approach

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Journal of Mountain Science 2017年第8期14卷 1550-1562页

作者： cui Sheng-hua PEI Xiang-jun huaNG Run-qiu State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology

The effects of slope surface material, slope gradient, block shape, and block mass conditions on rockfall rolling velocity were estimated with orthogonal test approach. Visual analysis shows that the importance of the factors is slope surface material > slope gradient > block shape > block mass. All the factors except block mass have the F value greater than the critical value, suggesting that these three factors are the key factors affecting the rockfall rolling velocity. Factor interaction analysis shows that the effect of the slope gradient relies largely on the slope surface conditions, and the block shape has little influence if the slope gradient is larger than a critical value. An empirical model considering the three key factors is proposed to estimate the rolling velocity, of which the error is limited to 5% of the testing value. This model is validated by 73 field tests, and the prediction shows excellent correlation with the site test. Thus, this analysis can be used as a tool in the rockfall behavior analysis.

关键词： Rockfall Rolling motion Experiment approach Gentle slope Orthogonal test

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