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Journal of Environmental Sciences 2022年第3期34卷 356-364页

作者： hu cui Yang Ou Lixia Wang Baixing Yan Yingxin Li Meiwen Bao Key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China Jilin Provincial Engineering Center of CWs Design in Cold Region&Beautiful Country Construction Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China University of Chinese Academy of Sciences Beijing 100049China

Considerable research efforts have been devoted to increase phosphorus(P)availability during aerobic ***,there is little discussion weather the dissolved organic carbon(DOC)controls the transformation among ***,we investigated the changes in DOC compositions and P-fractions during biochar-amended composting(wet weight basis,5%and 10%).TP content continuously increased since the'concentration effect'during aerobic ***_(3)-Pi,NaOH-Pi and HCl-Pi were main P-fractions,and biochar can improve P-bioavailability by transforming NaOH-Pi and HCl-Pi into NaHCO_(3)-*** equation models(SEMs)indicated that biochar enhanced the P-bioavailability through regulating the decomposition of *** results at least hint that the activation mechanism on P under the influence of DOC during biochar-amended composting.

关键词： Co-composting Biochar Dissolved organic carbon Phosphorus fractions

Magnetism and Sound Velocities of Iron Carbide （Fe3C） under Pressure

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Chinese Journal of Chemical Physics 2014年第3期27卷 297-301页

作者： Zhao-yi Zeng cui-e hu Xun Liu Ling-cang Cai College of Physics and Electronic Engineering Chongqing Normal University Chongqing 400047China National Key Laboratory for Shock Wave and Detonation Physics Research Institute of Fluid Physics Chinese Academy of Engineering Physics Mianyang 521900 China Institute of Pulsed Power Science Kumamoto University Kurokami Kumamoto 860-8555 Japan

The elastic property and sound velocity of FeaC under high pressure are investigated by using the spin-polarized generalized gradient approximation within density-functional theory. It is found that the magnetic phase transition from the ground ferromagnetic （FM） state to the nonmagnetic （NM） state occurs at ～73 GPa. Based on the predicted hugoniot of Fe3C, we calculate the sound velocities of FM-Fe3C and NM-Fe3C from elastic constants. Compared with pure iron, NM-FeaC provides a better match of compressional and shear sound velocities with the seismic data of the inner core, supporting carbon as one of the light elements in the inner core.

关键词： Alloy Sound velocity Density functional theory

Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第2期12卷 93-102页

作者： Jia-ying Zhu Qi FANG Gong-yin YE cui hu State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathology and Insects of Ministry of Agriculture Institute of lnsect Sciences Zhejiang University Hangzhou 310029 China Key Laboratory of Forest Disaster Warning and Control of Yunnan Province and Key Laboratory of Southwest Mountain Forest Resources Conservation and Utilization of Ministry of Education Southwest Forestry University Kunming 650224 China

Parasitism by the endoparasitoid wasp Pteromalus puparum causes alterations in the plasma proteins of Pieris rapae. Analysis of plasma proteins using a proteomic approach showed that seven proteins were differentially expressed in the host pupae after 24-h parasitism. They were masquerade-like serine proteinase homolog (MSPH), enolase (Eno), bilin-binding protein (BBP), imaginal disc growth factor (IDGF), ornithine decarboxylase (ODC), cellular retinoic acid binding protein (CRABP), and one unknown function protein. The full length cDNA sequences of MSPH, Eno, and BBP were successfully cloned using rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that the transcript levels of MSPH and BBP in the fat bodies of host pupae were inducible in response to the parasitism and their variations were consistent with translational changes of these genes after parasitism, while the transcript levels of Eno and IDGF were not affected by parasitism. This study will contribute to the better understanding of the molecular bases of parasitoid-induced host alterations associated with innate immune responses, detoxification, and energy metabolism.

关键词： Proteomics Plasma Parasitism Gene cloning Pteromalus puparum Pieris rapae

Detection of food source by PCR analysis of the gut contents of Aldrichina grahami （Aldrich）（Diptera： Calliphoridae） during post-feeding period

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Insect Science 2007年第1期14卷 47-52页

作者： KAI LI GONG-YIN YE JIA-YING Zhu cui hu Institute of Insect Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou China

The last meal of sarcophagous maggots may be useful in identifying the species on whose flesh they have fed （the＂host＂species）. The DNA profile of the host species may indeed be detectable in the＂last meal＂. In this paper, mitochondrial DNA analysis of gut contents was used to identify the prior host of post-feeding larvae of Aldrichina grahami （Aldrich）（Diptera： Calliphoridae）. A modified logistic equation was fitted to estimate the probability of identifying the host under five different constant temperatures （16, 20, 24, 28 and 32 ℃）. Our results shows that the detected time ranged from a maximum of 24 h at 32℃ to 42 h at 16℃ and a minimum of 12 h at 32~C to 30 h at 16℃. Furthermore, the host detection time was also calculated to give the maximal time after larval hatching from the egg. These results indicate that, in criminal cases where the maggots stray from the corpse, the last meal of the larvae should not be overlooked as potentially critical evidence.

关键词： Cytochrome b gene detectable time forensic entomology gut post-feeding

Proteome changes in the plasma of Papilio xuthus (Lepidoptera:Papilionidae):effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera:Pteromalidae)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第6期10卷 445-453页

作者： Jia-ying Zhu Gong-yin YE Qi FANG cui hu State Key Laboratory of Rice Biology Institute of Insect Sciences Zhejiang University Hangzhou 310029 China

Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects.

关键词： Proteomics Plasma Parasitism Immune response Pteromaluspuparum Papilio xuthus

Heat shock protein genes （hsp20, hsp75 and hsp90） from Pieris rapae： Molecular cloning and transcription in response to parasitization by Pteromalus puparum

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Insect Science 2013年第2期20卷 183-193页

作者： Jia-Ying Zhu Guo-Xing Wu Gong-Yin Ye cui hu Key Laboratory of Forest Disaster Warning and Control of Yunnan Province Southwest Forestry University Kunming College of Plant Protection Yunnan Agricultural University Kunming Key laboratory of Molecular Biology of Crop Pathology and Insects of Ministry of Agriculture Institute of Insect Sciences Zhejiang University Hangzhou China

Most molecular work on the roles of heat shock proteins （hsps） in host-parasite interaction has focused on vertebrates, rather than invertebrates. Here the full length complementary DNA （cDNA） sequences of three hsp genes （hsp20, hsp75 and hsp90） were amplified from Pieris rapae, and their transcriptional responsiveness to parasitization by the endoparasitic wasp Pteromalus puparum were investigated. The cDNA sequence analysis of hsp20, hsp75 and hsp90 revealed open reading frames of 531, 2328 and 2 157 bp in length, which encode proteins with calculated molecular weights of 19.5, 75.48 and 82.7 kDa, respectively. The comparison of amino acid sequences showed that P rapae hsp20 shared highly divergent homology to that of other insects, while hsp75 and hsp90 showed high homology to their counterparts of other species. The expression analysis indicated that these three genes were influenced in response to parasitization by P. puparum. The hsp20 transcripts in parasitized pupae were higher compared to non- parasitized pupae. The expression of hsp75 and hsp90 were down-regulated following parasitization. The results indicate that hsps are involved in host-parasitoid interactions.

关键词： gene expression heat shock protein parasitization

Effects and complications of placement of motility coupling post in porous polyethylene orbital implants

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第6期9卷 464-469页

作者： Hong-guang cui Rong-rong hu hui-yan LI Wei HAN Department of Ophthalmology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Objective： To investigate the effects and complications of primary and secondary placements of motility coupling post （MCP） in the unwrapped porous polyethylene orbital implant （PPOI） following enucleation. Methods： We investigated 198 patients who received PPOI implantation following the standard enucleation procedure in the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China, from 2002 to 2004. These patients were subgrouped into PPOI-only patients （112 cases, received PPOI following enucleation）, primary MCP patients （46 cases, received primary placement of MCP during PPOI operation）, and secondary MCP patients （40 cases, received secondary placement ofMCP 6 months after the initial surgery）. Effects and complications among these three groups were compared. Results： The PPOI-only patients took shorter treatment course when compared with other two MCP groups （P〈0.001）, without significant difference noted between the two MCP groups. However, the two MCP groups had better prosthetic motility than PPOI-only group （P〈0.001）, without significant difference between the two MCP groups. In the early stage, 2 eyes in the PPOI-only group and l eye in the primary MCP group had PPOI infection. In PPOI-only group, 3 （2.68%） eyes had PPOI exposure, which occurred after fitting the prostheses; 4 eyes （8.70%） in primary MCP group and 1 eye （2.50%） in secondary MCP had PPOI exposure, which occurred before fitting the prostheses. After prosthesis was fit successfully, the excessive discharge and granuloma were 33.9% and 1.79% in PPOI group-only, 53.3% and 8.9% in primary MCP group, and 52.5% and 7.5% in secondary MCP group, respectively. Conclusion： Both primary and secondary placements of MCP into the PPOI following enucleation can help patients to obtain desirable prosthetic motility, but may be associated with more complications. The primary placement of MCP with skilled operation in selected patients is more recommendable than secondary placemen

关键词： Porous polyethylene orbital implant (PPOI) Motility coupling post (MCP)

Hydrogen production via catalytic decomposition of NH3 using promoted MgO-supported ruthenium catalysts

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Science China Chemistry 2019年第12期62卷 1625-1633页

作者： Xiu-cui hu Wei-Wei Wang Rui Si Chao Ma Chun-Jiang Jia Key Laboratory for Colloid and Interface Chemistry Key Laboratory of Special Aggregated MaterialsSchool of Chemistry and Chemical EngineeringShandong UniversityJinan 250100China Shanghai Synchrotron Radiation Facility Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201204China College of Materials Science and Engineering Hunan UniversityChangsha 410082China

Catalytic decomposition of NH3 to high purity hydrogen offers a promising strategy for fuel cells, but presents challenges for high hydrogen yields at comparatively low temperatures due to the lack of efficient catalysts. Here, we report the facile preparation of ultra-fine ruthenium（Ru） species dispersed on Mg O, which show excellent activity and high temperature stability for NH3 decomposition reaction. The hydrogen yield of the prepared Ru/Mg O catalysts reaches ca. 2,092 mmol H2 g–1 Ru min–1 at450 °C, far exceeding that of the previously reported most reactive Ru-based catalysts and the same chemical composition samples prepared by other approaches. Various characterization techniques containing X-ray absorption fine structure（XAFS）,in-situ diffuse reflectance infrared Fourier transform spectroscopy（in-situ DRTFTS） and temperature-programmed reduction/desorption（TPR/TPD） were carried out to explore the structure-function relation of the prepared Ru/Mg O catalysts. We found that the Ru species interact strongly with the Mg O support, which can efficiently protect the Ru species and Mg O support from agglomerating during NH3 decomposition test, maintaining the stability of the catalysts.

关键词： Ru catalyst MgO support ammonia decomposition hydrogen production structure-activity relation

Influence of age on prevalence rates of chronic complications of hospitalized aged patients with hypertension -- analysis of 17,682 cases

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Journal of Geriatric Cardiology 2009年第2期6卷 91-94页

作者： hua cui Yixin hu Li Fan Guoliang hu Wei Dai The second department of the Geriatric Cardiology Chinese PLA General Hospital Beijing 100853China

Objective To understand the relationship between age and chronic complications in hospitalized aged patients with hypertension, to provide evidence for hypertension prevention and control. Methods To retrospectively analyze the clinical and laboratory data on 17,682 patients with essential hypertension during Jan 1 s,, 1993-Dec 12th, 2008 in PLA general hospital. Results 1） Among all of the inrolled cases, those aged 60-64 account for 27.87%, 65-69 years group account for 26.55%, 70-74 years group accounted for 23.96%, 75-79 years group accounted for 14.14%, 80-84 years group accounted for 5.26%, 85-89 years group accounted for 1.69%, 〉 90 years accounted for 0.41%. 2） The prevalence rate of chronic complications in 60-69 years group were 31.3-31.2% for diabetes and,22.6-27.0% for cerebrovascular disease, 9.5-11.1% for myocardial infarction, 6.7-9.1% for heart failure, 5.8-6.0% for renal dysfanction 4.9-6.8% for atrial fibrillation, 0.1-0.3% for multiple organ dysfunction syndrome （MODS） in the elderly（P 〈0.05 ）. 3） The first four complications of hypertension were diabetes（33.5%）, cerebrovascular disease （31.9%）, myocardial infarction（13.2%） and heart failure（12.3%） in 70-74 years group （P〈0.05）, cerebrovascular disease （42.8%）, diabetes （32.8%）, heart failure （16.5%） and myocardial infarction（15.9%） in 75-79 years group （P〈0.05）, cerebrovascular disease （45.4%）, diabetes （35.0%）, heart failure （21.1%） and myocardial infarction（l 5.9%） in 80-84 years group （P〈0.05）, cerebrovascular disease（42.5%）, diabetes （35.8%）, heart failure （23.1%） and renal dysfanction （17.7%） in 85-89 years group（P〈0.05 ）,and cerebrovascular disease （45. 2%）, heart failure（31.5%） , diabetes （26.0%） and renal dysfanction （20.5%） in patients more than 90 years group （P〈0.05）. Conclusions The prevalence rate and kinds of chronic complications in hospitalized aged patients with hypertension were changed with the increasing age, and the first kind of complication is cerebrovascular disease. It is

关键词： Hypertension stroke myocardial infarction outcome

Regional ecosystem changes under different cascade hydropower dam construction scenarios in the LRGR

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Chinese Science Bulletin 2007年第A02期52卷 106-114页

作者： ZHAI HongJuan cui BaoShan hu Bo WEI GuoLiang LIU ShiLiang State Key Joint Laboratory of Environmental Simulation and Pollution Control School of Environment Beijing Normal UniversityBeijing 100875 China

生态系统上的串联水力发出的电力水坝构造的生态的效果与在纵的范围峡区域膨胀水坝发展规模从科学家获得了许多担心。在 Lancang 河区域和纵的范围峡区域(LRGR ) 的 Yuanjiang River-Red 河区域，一个索引系统因此被建立了估计当前的生... 详细信息

生态系统上的串联水力发出的电力水坝构造的生态的效果与在纵的范围峡区域膨胀水坝发展规模从科学家获得了许多担心。在 Lancang 河区域和纵的范围峡区域(LRGR ) 的 Yuanjiang River-Red 河区域，一个索引系统因此被建立了估计当前的生态的危险并且预言可能的未来在水坝构造以后的这二个河区域的生态的危险。八种串联水力发出的电力水坝构造情形为预报在这份报纸被建立了。生态系统的突然的变化特征在计算被考虑了。象水坝构造的当前的供应，航行和旅游一样的经济好处没在计算被考虑。(1 ) 当前的生态的危险 Lancang 河区域和 Yuanjiang River-Red 河区域珍视的结果表演分别地是 0.34 和 0.43，它将在所有计划水坝的联合工作以后分别地升起到 0.900.91 和 0.860.89；(2 ) 突然的变化将在未来发生在 Lancang 河区域和 Yuanjiang River-Red 河区域身上；(3 ) 有高人的反应的间隔建设为 Lancang 河区域和 Yuanjiang River-Red 河区域，他们的多半生态的危险将分别地在以后升起到 0.560.62 和 0.420.43 被推荐。这些结果提供一个合理方法和理论参考书估计水坝建设的生态的效果，并且也把 prewarnings 给正在被计划构造串联水力发出的电力水坝的纵的范围峡区域的另外的主流。

关键词：纵向岭谷区地区生态系统变化模式瀑布