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Bioactive Compounds Recovery from Larrea tridentata by Green Ultrasound- Assisted Extraction

Phyton-International Journal of Experimental Botany 2024年第11期93卷 3091-3107页

作者： Muyideen Olaitan Bamidele Jose Sandoval-Cortes Maria Liliana Flores-Lopez Olga Berenice Alvarez Perez Monica Lizeth Chavez Gonzalez cristobal noe.aguilar Bioprocesses and Bioproducts Research Group Food Research DepartmentSchool of ChemistryUniversidad Autonoma de CoahuilaSaltillo25070Mexico Centro de Investigacion e Innovacion Cientifica y Tecnologica Universidad Autonoma de CoahuilaSaltillo25070Mexico

Postharvest losses remain a signiﬁcant challenge,particularly in developing countries that have inadequate *** plants offer an eco-friendly and sustainable solution for managing diseases and pests in agri-cultural *** plants are rich in bioactive compounds,such as alkaloids,ﬂavonoids,terpenoids,phenolics,and essential oils,many of which have proven antimicrobial,antifungal,insecticidal,and antioxidant *** characteristics make medicinal plants viable candidates for postharvest disease and pest *** these,Larrea tridentata(creosote bush)is particularly notable for its bioactive compounds with strong antifungal *** potential applications include agriculture,food preservation,and *** study aimed to evaluate how different solvent mixtures affect the efﬁciency of ultrasound-assisted extraction,total polyphenol content,antioxidant capacity,and antifungal activity of *** leaves and ***ﬁndings revealed that the 60%ethanol ultrasound-assisted extract of *** leaves(ULL 60%)contained the highest concentration of bioactive compounds,including hydrolysable tannins(690.2 mg GAE/100 g)and condensed tan-nins(329.9 mg CE/100 g).All extracts demonstrated notable antioxidant activity in ABTS,DPPH,and FRAP assays,with ethanol-based extracts showing greater antioxidant potential than their aqueous *** terms of antifungal efﬁcacy,100%ethanol leaf extract exhibited the strongest inhibition against Fusarium oxyspor-um(60.03%),whereas 50%ethanol extract effectively inhibited Alternaria alternata(53.61%).Six major polyphe-nolic compounds were identiﬁed using reverse-phase high-performance liquid chromatography coupled with electrospray ionization mass spectrometry(RP-HPLC-ESI-MS).These include quercetin,luteolin,3,4-dihydrox-yphenylethanol,elenolic acid,nordihydroguaiaretic acid(NDGA),and *** compounds are known to have antibacterial,antifungal and antioxidant propert

关键词： Phytopathogens bioactive compounds desert plants Fusarium oxysporum Alternaria alternata

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In-vitro assessment for the control of Fusarium species using a lactic acid bacterium isolated from yellow pitahaya(Selenicereus megalanthus(K. Schum. Ex Vaupel Moran))

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Journal of Integrative Agriculture 2021年第1期20卷 159-167页

作者： Leidy J.VALENCIA-HERNANDEZ Karina LÓOPEZ-LÓPEZ Eyder D.GÓMEZ-LÓPEZ Liliana SERNACOCK cristobal N.aguilar Universidad Nacional de Colombia Palmira CampusValle 763533Colombia Food Research Department School of ChemistryAutonomous University of CoahuilaSaltillo CampusCoahuila 25280Mexico

The fungistatic activity of a lactic acid bacterium, which had been isolated from yellow pitahaya cultures, against fungi associated with basal rot(Fusarium oxysporum and Fusarium fujikuroi) was measured in the present study. Its activity was assessed in three fractions: fermented(S1), metabolic products(S2), and biomass(S3), using two fermentation substrates: Man Rogosa Sharpe agar(MRS) and potato dextrose agar(PDA). The bacterium was molecularly identified as Lactobacillus plantarum. S3 reduced F. fujikuroi growth by 100% over 48 h of fermentation, which occurred during the stationary phase of bacterial growth. The three fractions' fungistatic activity against F. fujikuroi depended on the substrate employed. The fermentation kinetic parameters for L. plantarum indicated that its specific growth rate was 0.46 h^–1, with 93.63% substrate consumption, 0.045 kg kg^–1 cell yield, and 0.54 kg kg^–1 product yield. The kinetic parameters calculated will allow for bacteria production scaling. These in-vitro trials reveal L. plantarum's possible application as a biocontrol agent for diseases associated with Fusarium. However, further ex-vivo and in-vivo researches are required to demonstrate its behavior in crops.

关键词： lactic acid bacteria Fusarium fungistatic activity biomass yellow pitahaya

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Development and characterization of whey protein films incorporated with tarbush polyphenols and candelilla wax

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Food Bioscience 2022年第1期45卷 1-7页

作者： Olga B.Alvarez-Perez Janeth M.Ventura-Sobrevilla Cristian Torres-Leon Romeo Rojas-Molina Raúl Rodríguez-Herrera Miguel A.aguilar-Gonzalez cristobal N.aguilar Bioprocesses and Bioproducts Research Group Food Research DepartmentSchool of ChemistryUniversidad Autonoma de Coahuila25280SaltilloCoahuilaMexico School of Health Sciences Universidad Autonoma de Coahuila26090Piedras NegrasCoahuilaMexico School of Agronomy Research Center and Development for Food IndustryUniversidad Autonoma de Nuevo Leon6050General EscobedoNuevo LeonMexico Center for Research and Advanced Studies of the National Polytechnique Institute Parque Industrial Saltillo-Ramos ArizpeCoahuilaMexico

Edible films were prepared with whey protein incorporating candelilla wax,and glycerol in aqueous *** polyphenols were added as bioactive *** optimization based on the water vapor permeability(WVP)of the formulation was performed using a Box-Behnken *** films were characterized by the determination of WVP,moisture,solubility,color,antioxidant and antifungal *** electron microscopy(SEM)was also performed to observe the surface and cross-section *** lowest permeability values were found with concentrations of 1.27,0.3 and 0.5% of protein,wax and glycerol *** was proved that the addition of polyphenols into films causes a significant effect only on solubility and transparency *** films have suitable properties as a barrier for the goods they could be applied *** is proposed for use in foods such as fresh or minimally processed fruits and vegetables.

关键词： Edible films Whey protein Shelf life Candelilla wax Euphorbia antisyphilitica Fluorensia cernua polyphenols

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Bacteriocins as antimicrobial and preservative agents in food:Biosynthesis,separation and application

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Food Bioscience 2022年第2期46卷 749-771页

作者： Deepak Kumar Verma Mamta Thakur Smita Singh Soubhagya Tripathy Alok Kumar Gupta Deepika Baranwal Ami R.Patel Nihir Shah Gemilang Lara Utama Alaa Kareem Niamah M´onica L.Ch´avez-Gonz´alez Carolina Flores Gallegos cristobal noe.aguilar Prem Prakash Srivastav Agricultural and Food Engineering Department Indian Institute of Technology KharagpurKharagpur721302West BengalIndia Department of Food Technology School of SciencesITM UniversityGwalior474001Madhya PradeshIndia Department of Nutrition and Dietetics University Institute of Applied Health SciencesChandigarh UniversityChandigarh140413PunjabIndia Division of Post-harvest Management ICAR-Central Institute for Subtropical Horticulture(Ministry of Agriculture and Farmers WelfareGovernment.of India)Lucknow(UP)226101India Department of Home Science Arya Mahila PG CollegeBanaras Hindu UniversityVaranasiUttar Pradesh221005India Division of Dairy Microbiology Mansinhbhai Institute of Dairy and Food Technology-MIDFTDudhsagar Dairy CampusMehsana384002GujaratIndia Faculty of Agro-Industrial Technology Universitas PadjadjaranSumedang45363Indonesia Center for Environment and Sustainability Science Universitas PadjadjaranBandung40132Indonesia Department of Food Science College of AgricultureUniversity of BasrahBasra CityIraq Bioprocesses and Bioproducts Research Group and Nanobioscience Group.Food Research Department School of Chemistry.Autonomous University of CoahuilaSaltillo Campus25280CoahuilaMexico

Emerging evidence shows the trend of using safe and natural preservatives like bacteriocins in food *** increasing demand by the food industry to extend keeping quality and prevent spoilage of various food products has appealed for new preservatives and new methods of ***,to discover the novel spectrum of antimicrobial compounds that could effectively battle food-borne pathogens,bacteriocins have been intensively studied in the last few ***,great attention has been paid to the application of lactic acid-bacterial bacteriocins targeting food spoilage-causing or pathogenic microorganisms with no significant side *** mechanisms of action like pore-formation,retarding cell-wall/nucleic acid/protein synthesis have been proposed and described for diverse ***,the syntheses,purification of bacteriocins in addition to their applications as antimicrobial and preservative agents in food processing are reviewed in *** review will also discuss the preservative effect of bacteriocins and their combinations having multiple modes of action(hurdle approach)to reduce the microbial load as well as antimicrobial resistance.

关键词： Lactic acid bacteria Bacteriocins Preservative Food-borne pathogens Food processing

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Polyurethane as substrate for fungal strains

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Advances in Bioscience and Biotechnology 2011年第2期2卷 52-58页

作者： Araceli Loredo-Trevino Gilberto Garcia Abraham Velasco-Tellez Raul Rodriguez-Herrera cristobal N.aguilar Group of Bioprocesses.School of Chemistry.University of Coahuila.Saltillo Mexico Research and Development Division.Nemak SA GarciaN.L.Mexico Biotechnology Department.School of Chemistry.University of Coahuila.Saltillo Mexico

Polyurethane is a versatile plastic with several industrial applications in the modern life, but it is considered as a very recalcitrant material. Biodegradation of this plastic has been poorly explored, and most of the studies that have been published focus on bacterial enzymes. In this work, some fungi with the capacity of growing with polyurethane foam as nutrient source were isolated from sands contaminated with this plastic and from DIA/UAdeC collection, testing their ability to grow on polyurethane as sole carbon and nitrogen sources and their enzymatic activities were determined in specific media as well as their invasion capacity on polyurethane agar plates. 22 fungal strains demonstrated their capacity of growing on polyurethane. Among the enzymatic activities evaluate, the most common was the urease activity (95% of the strains).Protease, esterase and laccase activities were present in 86%, 50% and 36% respectively. The great ability of the isolated fungal strains to use polyurethane foam as nutrient opens an important opportunity to study at detail the biodegradation of this plastic, with clear implications in cell biology and environmental technology.

关键词： Polyurethane Biodegradation Fungal Enzymes In-Plate Screening

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The optimization of phenolic compounds extraction from cactus pear(Opuntia ficus-indica) skin in a reflux system using response surface methodology

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Asian Pacific Journal of Tropical Biomedicine 2013年第6期3卷 436-442页

作者： Aguirre Joya Jorge De La Garza Toledo Heliodoro Zugasti Cruz Alejandro Belmares Cerda Ruth aguilar Cristóbal No Food Research Department School of ChemistryUniversidad Autonóma de Coahuila Basic Science Department Universidad Autonóma Agraria Antonio Narro

Objective:To extract,quantify,and evaluate the phenolic content in Opuntia ficus-indica skin for their antioxidant capacity with three different methods(ABTS,DPPH,and lipid oxidation)and to optimize the extraction conditions[time,temperature and ethanol concentration)in a reflux ***:The extraction process was done using a reflux system.A San cristobalⅡexperimental design with three variables and three levels was *** variables evaluated were time of extraction(h),concentration of ethanol(%,v/v)and temperature(°C).The extraction process was optimized using a response surface ***:It was observed that at higher temperature more phenolic compounds were extracted,but the antioxidant capacity was *** optimum conditions for phenolic compounds extraction and antioxidant capacity mixing the three methods were as Follows:45%of ethanol,80℃and 2 hours of *** obtained in our results are little higher that other previously ***:It can he concluded the by-products of Opuntia ficus-indica represent a good source of natural antioxidants with possible applications in food,cosmetics or drugs industries.

关键词： Opuntia ficus-indica Reflux Phenolics Antioxidants Prickly pear Response surface methodology

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