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Genetic mapping and survey of powdery mildew resistance in the wild Central Asian ancestor of cultivated grapevines in Central Asia

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Horticulture Research 2020年第1期7卷 1444-1458页

作者： Summaira Riaz cristina m.menéndez Alan Tenscher Daniel Pap m.Andrew Walker Department of Viticulture and Enology University of CaliforniaDavisCA 95616USA Instituto de Ciencias de la Vid y del Vino(ICVV) Universidad de La Rioja-CSICGobierno de La RiojaCarretera de Burgos Km6Finca La GrajeraLogroñoLa Rioja 26007Spain

Cultivated grapevines(Vitis vinifera)lack resistance to powdery mildew(Pm)with few *** to this pathogen within *** has been reported in earlier studies and identified as the Ren1 locus in two Central Asian table grape *** Pm-resistant cultivated varieties and accessions of the wild ancestor *** *** were soon identified raising questions regarding the origin of the *** this study,F1 breeding populations were developed with a Pm susceptible *** *** breeding line and a Pm-resistant *** *** was carried out with five Ren1 locus linked SSR markers.A Pm resistance locus explaining up to 96%of the phenotypic variation was identified in the same genomic position,where the Ren1 locus was previously *** SSR marker alleles linked with the resistance locus were *** report results of Pm resistance in multiple accessions of *** collected as seed lots or cuttings from five countries in the Caucasus and Central Asia.A total of 20 females from 11 seed lots and 19 males from nine seed lots collected from Georgia,Armenia,and Azerbaijan were resistant to *** male and one female plant collected as cuttings from Afghanistan and Iran were also resistant to *** analysis of markers linked with the Ren1 locus in conjunction with disease evaluation data found a high diversity of allelic haplotypes,which are only possible via recombination events occurring over a long time *** analysis of two alleles of the SSR marker that cosegregates with the resistance found SNPs that were present in the wild progenitor and in cultivated *** levels of Pm resistance among the tested accessions were also *** lines of evidence suggest that the powdery mildew fungus may have been present in Asia for a longer time than currently thought,giving the wild progenitor *** *** time to coevolve with and develo

关键词： breeding resistance Central

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Liquid biopsy for gastric cancer:Techniques,applications,and future directions

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World Journal of Gastroenterology 2024年第12期30卷 1680-1705页

作者： cristina Díaz del Arco m Jesús Fernández Aceñero Luis Ortega medina Department of Surgical Pathology Health Research Institute of the Hospital Clínico San CarlosHospital Clínico San CarlosMadrid 28040Spain Department of Legal medicine Psychiatry and PathologyUniversidad Complutense de MadridMadrid 28040Spain

After the study of circulating tumor cells in blood through liquid biopsy(LB),this technique has evolved to encompass the analysis of multiple materials originating from the tumor,such as nucleic acids,extracellular vesicles,tumor-educated platelets,and other ***,research has extended to include the examination of samples other than blood or plasma,such as saliva,gastric juice,urine,or *** techniques are diverse,intricate,and *** must be highly sensitive,and pre-analytical,patient,and tumor-related factors significantly influence the detection threshold,diagnostic method selection,and potential ***,the implementation of LB in clinical practice still faces several *** potential applications of LB range from early cancer detection to guiding targeted therapy or immunotherapy in both early and advanced cancer cases,monitoring treatment response,early identification of relapses,or assessing patient *** the other hand,gastric cancer(GC)is a disease often diagnosed at advanced *** recent advances in molecular understanding,the currently available treatment options have not substantially improved the prognosis for many of these *** application of LB in GC could be highly valuable as a non-invasive method for early diagnosis and for enhancing the management and outcomes of these *** this comprehensive review,from a pathologist’s perspective,we provide an overview of the main options available in LB,delve into the fundamental principles of the most studied techniques,explore the potential utility of LB application in the context of GC,and address the obstacles that need to be overcome in the future to make this innovative technique a game-changer in cancer diagnosis and treatment within clinical practice.

关键词： Liquid biopsy Gastric cancer Circulating tumor cells Cell-free DNA Circulating tumor DNA molecular

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Unveiling sinus venosus atrial septal defect and partial anomalous pulmonary venous return in an elderly patient

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Journal of Geriatric Cardiology 2024年第4期21卷 462-464页

作者： cristina m Font Anamarys Blanco Fernandez Dinesh Kadariya Department of medicine Division of CardiologyUniversity of Florida College of MedicineJacksonvilleFloridaUSA

Sinus venosus defect(SVD) accounts for 4%–11% of all atrial septal defects(ASD) and may present with dyspnea, heart failure(HF), palpitations, or angina.^[1] The malformation typically involves an interatrial communication due to a deficiency in the common wall connecting the right pulmonary veins and the superior vena cava(SVC) and often accompanies anomalous pulmonary venous connection of some or all of the pulmonary veins.

关键词： atrial septal venous

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In‑depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1027-1055页

作者： maria A.Gil Josep m.Cambra Heriberto Rodriguez‑martinez cristina Cuello Inmaculada Parrilla Emilio A.martinez Department of medicine and Animal Surgery International Excellence Campus for Higher Education and Research“Campus Mare Nostrum”University of MurciaMurciaSpain Institute for Biomedical Research of murcia(ImIB-Pascual Parrilla) MurciaSpain Technical University of munich MunichGermany Department of Biomedical&Clinical Sciences(BKV) BKH/Obstetrics&GynaecologyLinkoping UniversityLinkopingSweden

Background Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo *** this study,the proteome of the endometrium and cho-rioallantoic membrane was characterized in pregnant sows(PS)during early gestation(d 18 and 24 of gestation)and in the endometrium of non-pregnant sows(NPS)during the same days using LC-mS/mS *** UniProtKB database and ClueGO were used to obtain functional Gene Ontology annotations and biological and functional networks,*** Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS,respectively;of these,1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS,*** addition,we iden-tified 3,968 proteins in the extraembryonic membranes of *** analyses of function revealed some proteins had relevance for the immune system process and biological adhesion in endometrium while the embryonic chorion displayed abundance of proteins related to cell adhesion and cytoskeletal organization,suggesting they dominated the m.ment of endometrial remodeling,implantation and adhesion of the lining *** are available via Pro-teomeXchange with identifier *** This is the first in-depth proteomic characterization of the endometrium and extraembryonic mem-branes during weeks 3 to 4 of gestation;data that contribute to the molecular understanding of the dynamic environ-ment during this critical period,associated with the majority of pregnancy losses.

关键词： Endometrium Extraembryonic membranes Implantation Pig Proteome

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Synthesis and applications of lanthanoid complexes of pentadentate and hexadentate N_(5) and N_(6) macrocycles:A review

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Journal of Rare Earths 2024年第1期42卷 1-15,I0001页

作者： Julio Corredoira-Vázquez cristina González-Barreira Paula Oreiro-martínez Ana m.García-Deibe Jesús Sanmartín-matalobos matilde Fondo Departamento de Química Inorganica Facultade de QuímicaUniversidade de Santiago de CompostelaCampus Vida15782Santiago de CompostelaSpain Phantom-g CICECO-Aveiro Institute of MaterialsDepartment of PhysicsUniversity of Aveiro3810-193AveiroPortugal Institute of materials(imATUS) Universidade de Santiago de Compostela15782Santiago de CompostelaSpain

In this paper,the lanthanoid complexes of N_(5) and N_(6) macrocycle ligands,without pendant arms or additional heteroatoms,are *** review covers the period from 2015 to the current date,since in 2014 Schiff base macrocyclic ligands incorporating the pyridine moiety,and their complexes,were revised,and in 2015 the chemistry of pentaaza macrocycle ligands with rare earth metals was also *** and phthalocyanine ligands are not included in this review,which primarily focuses on complexes with Schiff bases and amines without pendant arms or any additional donor other than *** synthetic methods,structural characterization,based on single X-ray crystal data,and properties of the lanthanoid complexes,with special attention to magneto-structural correlations,are presented herein.

关键词： Lanthanoid N_(5),N_(6)macrocycle Coordination chemistry Chirality Biological activity molecule magnet

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Extreme drought triggers parallel shifts in wood anatomical and physiological traits in upper treeline of the mediterranean Andes

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Ecological Processes 2024年第1期13卷 132-143页

作者： Luiz Santini Jr. Dylan Craven Daigard Ricardo Ortega Rodriguez manolo Trindade Quintilhan Stephanie Gibson-Carpintero cristina Aravena Torres Fidel A.Roig Ariel A.mu?oz Alejandro Venegas-Gonzalez Hémera Centro de Observación de la Tierra Universidad Mayor GEmA Center for Genomics Ecology and Environment Universidad Mayor Data Observatory Foundation ANID Technology Center Depart-ment of Forest Sciences ESALQ-University of Sao Paulo Department of Plant Biology Institute of Biology University of Campinas Instituto de Geografía Universidad Nacional Autónoma de México (UNAM) Argentine Institute of Nivology Glaciology and Environmental Sciences(IANIGLA CONICET ) Institute of Geography Pontificia Universidad Católica de Valparaíso Instituto de Ciencias Agroalimentarias Animales y Ambientales (ICA3) Universidad de O'Higgins

Background Treeline ecotones of mediterranean ecoregions have been affected by the increasing intensity and severity of droughts. Even though the effect of droughts on forest dynamics has been widely documented, knowledge is relatively scarce of how extreme climate episodes affect the hydraulic structure and, therefore, the phys-iology of woody plants. The mediterranean Andes have experienced an uninterrupted period of drought since 2010, including an extremely dry year in 2019 with approximately 80% rainfall deficit. Here, we investigated shifts in wood anatomical and physiological traits of Kageneckia angustifolia, an endemic treeline species, in response to this drought *** We evaluated the xylem plasticity of three K. angustifolia populations across their natural distribution(31–35° SL) based on anatomical(vessel structure and distribution) and physiological(intrinsic water-use efficiency) variables in the tree rings. We focused on the period 2000–2020 that corresponds to before the megadrought(2000–2007),(ii) megadrought(2008–2018) and(iii) hyperdrought(2019–2020). The variables were annualized and analyzed by linear mixed-effects *** Our results provide insights to the anatomical and physiological mechanisms underlying the resilience of treeline forests to persistent droughts in central Chile. We found that the extreme drought in 2019–2020 triggered shifts in vessel size and frequency that increased hydraulic safety. These significant shifts in vessel traits occurred in parallel with a decrease in pit aperture area and an increase in water-use efficiency, further increasing the resilience of K. angustifolia to extreme drought *** Our results revealed coordinated shifts in vessel size and frequency and water-use efficiency in response to the megadrought, thereby reducing vulnerability to hydraulic failure. The apparent resilience of K. angustifolia to extreme droughts suggests that this adaptation to drought stress may incre

关键词： Dendro-anatomy Kageneckia angustifolia Xylem vulnerability Drought Climate change

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Epidemiology,therapy and outcome of hepatocellular carcinoma between 2010 and 2019 in Piedmont,Italy

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 761-772页

作者： Christian Bracco marta Gallarate marco Badinella martini Corrado magnino Salvatore D'Agnano Roberta Canta Giulia Racca Remo melchio cristina Serraino Valentina Polla mattiot Giovanni Gollè Luigi Fenoglio Department of Internal medicine Santa Croce e Carle General HospitalCuneo 12100Italy Department of medical Sciences "City of Health and Science"University HospitalTorino 10100Italy

BACKGROUND Hepatocellular carcinoma(HCC)is the most common primary liver malignancy and the second leading cause of cancer deaths *** is often diagnosed at an advanced stage and therefore its prognosis remains poor with a low 5-year survival *** patients have increasingly complex and constantly changing characteristics,thus up-to-date and comprehensive data are *** To analyze the epidemiology and main clinical characteristics of HCC patients in a referral center hospital in the northwest of Italy between 2010 and *** In this retrospective study,we analyzed the clinical data of all consecutive patients with a new diagnosis of HCC recorded at"Santa Croce e Carle"Hospital in Cuneo(Italy)between 1 January 2010 and 31 December *** highlight possible changes in HCC patterns over the 10-year period,we split the population into two 5-year groups,according to the diagnosis period(2010-2014 and 2015-2019).RESULTS Of the 328 HCC patients who were included(m/F 255/73;mean age 68.9±11.3 years),154 in the first period,and 174 in the *** C virus infection was the most common HCC risk factor(41%,135 patients).The alcoholic etiology rate was 18%,the hepatitis B virus infection etiology was 5%,and the non-viral/non-alcoholic etiology rate was 22%.The Child-Pugh score distribution of the patients was:class A 75%,class B 21%and class C 4%.The average mayo end-stage liver disease score was 10.6±3.7.A total of 55 patients(17%)were affected by portal vein thrombosis and 158(48%)by portal *** average nodule size of the HCC was 4.6±3.1 cm.A total of 204 patients(63%)had more than one nodulemetastatic stage of the *** Barcelona Clinic Liver Cancer(BCLC)staging distribution of all patients was:4%very early,32%early,23%intermediate,34%advanced,and 7%*** survival rate was 1.6±0.3 *** 20%of the patients underwent ***,presence of ascites,BCLC stage and therapy w

关键词： Hepatocellular carcinoma Hepatitis virus Alcoholic hepatitis Non-alcoholic fatty liver disease Diagnosis Etiology Treatment

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Human leukocyte antigen compatibility and incidence of donorspecific antibodies in pediatric liver transplant recipients

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World Journal of Gastroenterology 2024年第33期30卷 3837-3845页

作者： melina U melere Flavia H Feier Jorge Neumann Antônio N Kalil Juliana de m montagner Luiza S Nader Carolina S da Silva marco Aurélio F Junior Gabriela P Coral Guilherme P Bobsin cristina T Ferreira Department of Hepatology and Liver Transplantation Santa Casa de Porto AlegrePorto Alegre 90050170Rio Grande do SulBrazil Department of Hepato-biliary-pancreatic Surgery and Liver Transplantation Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil Postgraduation Program in medicine:Hepatology Federal University of Health Sciences of Porto AlegrePorto Alegre 90050-170Rio Grande do SulBrazil Laboratory of Transplantation Immunology Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil

BACKGROUND Antibody-mediated rejection following liver transplantation(LT)has been increasingly recognized,particularly with respect to the emergence of de novo donor-specific antibodies(DSAs)and their impact on graft *** substantial evidence for adult populations exists,research focusing on pediatric LT outcomes remains *** To investigate the prevalence of human leukocyte antigen(HLA)mismatches and DSA and evaluate their association with rejection episodes after pediatric *** A cohort of pediatric LT recipients underwent HLA testing at Santa Casa de Porto Alegre,Brazil,between December 2013 and December *** patients who survived for>30 days after LT with at least one DSA analysis were *** classes I and II and cross-matches were *** presence of de novo DSA(dnDSA)was evaluated at least 3 months after LT using the Luminex®single antigen bead method,with a positive reaction threshold set at 1000 *** episodes were confirmed by liver *** Overall,67 transplanted children were analyzed;61 received grafts from living donors,85%of whom were related to ***-transplant DSA(class I or II)was detected in 28.3%of patients,and dnDSA was detected in 48.4%.The median time to DSA detection after LT was 19.7[interquartile range(IQR):4.3-35.6]*** rejection occurred in 13 patients at follow-up,with C4d positivity observed in 5/13 Liver *** median time to rejection was 7.8(IQR:5.7-12.8)*** presence of dnDSA was significantly associated with rejection(36%vs 3%,Pmonths were 76%vs 100%and 58%vs 95%for patients with dnDSA anti-DQ vs those without,*** Our findings highlight the importance of incorporating DSA assessment into pre-and post-transplantation protocols for pediatric LT *** implications may include immunosuppression minimization strategies based on this analysis in pediatric LT recip

关键词： Human leukocyte antigens Donor-specific antibodies Liver transplantation Pediatric Rejection

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A Novel and Functional Artificial Bruch’s membrane made of Poly (ε-Caprolactone)/Gelatin for Retinal Pigment Epithelium Restoration

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Advances in Bioscience and Biotechnology 2024年第8期15卷 443-461页

作者： López Edith Silva-Bermudez Phaedra Gonzalez-Salinas Roberto Velasquillo cristina Torres-Romero Abigail López-Colomé Ana maría Pérez Armando Gina Prado-Prone Edgar Ramos-martínez Iván Ramos-martínez Quiroz-mercado Hugo Hernández-Zimbrón Luis Fernando Instituto de Fisiologa Celular Universidad Nacional Autnoma de Mxico Ciudad de Mxico City Mxico Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa Instituto Nacional de Rehabilitación Luis Guiller-mo Ibarra Ibarra Ciudad de México México Departamento de Investigacin Asociacin Para Evitar la Ceguera Ciudad de Mxico Mxico Subdireccin de Investigacin Tecnolgica Instituto Nacional de Rehabilitacin Luis Guillermo Ibarra Ibarra Ciudad de Mxico Mxico Departamento de Biologa Celular y Tisular Facultad de Medicina Universidad Nacional Autnoma de Mxico Ciudad de Mxico Mxico Escuela de Ciencias Universidad Autnoma Benito Jurez de Oaxaca Oaxaca Mxico Departamento de medicina y Zootecnia de Cerdos Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional utnoma de Mxico Mxico Laboratorio de Investigacion Interdisciplinaria rea de Optometria Escuela Nacional de Estudios Superiores Unidad Len Universidad Nacional Autnoma de Mxico Len Guanajuato Mxico Laboratorio Nacional de ptica de la Visin-Centro de Investigaciones en ptica Len Guanajuato Mxico

Purpose: The primary aim of this paper is to design, create, and improve functional and artificial Bruch’s membranes (Bm) using bioengineering techniques, which can be applied in the treatment of maculopathies by supporting the growth and maintenance of retinal pigment epithelium (RPE) cells, thereby potentially enabling subretinal implantation in patients. methods: We fabricated by electrospinning ultrathin 3D nanofibrous membranes from Polycaprolactone (PCL), and different concentrations of gelatin (5%, 15% and 30%). ARPE-19 cells were seeded onto these artificial membranes. mTT assays were performed in order to evaluate ARPE-19 cell viability and cytotoxicity. IF assays were realized to observe the ARPE-19 cells onto each membrane. Ultrastructure of the modified Bruch’s membrane and ARPE-19 morphology after 25 days of culture were studied with transmission and scanning electron microscopy. To evaluate expression changes in markers of ARPE-19 (RPE65 and ZO-1) qRT-PCR assays were realized. Data from three independent experiments were pooled and expressed as the mean SD. A confidence level of P 0.05 was considered to be statistically significant. Results ARPE-19 cells grew on PCL/Gelatin membranes mainly in PCL/30% gelatin combination, which had not cytotoxic effect. RPE65/cytokeratin-18/ and actin-positive ARPE-19 cells formed a correctly orientated monolayer of polygonal cells with morphological polarity. The apical cell surfaces exhibited abundant protruding microfolds. moreover, zones of polygonal border look as if ARPE-19 cells fused among, suggesting the presence of tight junctions. The expression of RPE65 and ZO-1 was unchanged. Conclusions: PCL/30% Gelatine membranes may imitate the natural Bm to such extent that they support RPE-cells and exhibited RPE-like morphology. The engineering of a human RPE monolayer with these artificial Bm, emulating the in vivo retina, arouse their potential subretinal implantation in patients with wet age-related macular degene

关键词： Artificial Bruch’s membrane Electrospinning ARPE-19 Cells Age-Related macular Degeneration

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Variation in SARS-CoV-2 seroprevalence in children in the region of Asturias,Northern Spain

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World Journal of Pediatrics 2022年第12期18卷 835-844页

作者： Elisa García-García mercedes Rodríguez-Pérez David Pérez-Solís Carlos Pérez-méndez cristina molinos-Norniella Ángeles Cobo-Ruisánchez Eva maría Fernández Fernández Noelia García González Laura Calle-miguel Pediatrics Department Centro de Salud LaviadaCalle Juanín de Mieres833207GijónSpain microbiology Department Hospital Universitario Central de AsturiasOviedoSpain Pediatrics Department Hospital Universitario San AgustínAvilésSpain Pediatrics Department Hospital Universitario de CabueñesGijónSpain Pediatrics Department Hospital Carmen y Severo Ochoa.Cangas del NarceaSpain Pediatrics Department Centro de Salud TeatinosOviedoSpain Pediatrics Department Hospital Universitario Central de AsturiasOviedoSpain

Background Updated seroprevalence estimates are important to describe the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)landscape and to guide public health *** aims are to describe longitudinal changes in seroprevalence in children in a region in Northern Spain and to analyze factors associated with SARS-CoV-2 *** Prospective multicenter longitudinal study with subjects recruited from July to September ***(up to 14 years old)were included and followed up until September *** blood samples were collected every six months during three testing rounds and were analyzed for SARS-CoV-2 *** data regarding epidemiological features,contact tracing,symptoms,and virological tests were *** evolution of SARS-CoV-2 seroprevalence during the study and the differences between children with positive and negative SARS-CoV-2 antibody tests were *** Two hundred children were recruited(50.5%girls,median age 9.7 years).The overall seroprevalence increased from round 1[1.5%,95%confidence interval(CI)0.3%–4.3%]to round 2(9.1%,95%CI 4.6%–12.7%)and round 3(16.6%,95%CI 9.5%–19.6%)(Pmain changes occurred in children aged zero to four years(P=0.001)who lived in urban areas(Pme *** multivariable analysis,three variables independently associated with SARS-CoV-2 seropositivity were identified:close contact with coronavirus disease 2019(COVID-19)confirmed or suspected cases[odds ratio(OR)=3.9,95%CI 1.2–12.5],previous positive virological test(OR=17.1,95%CI 3.7–78.3)and fatigue(OR=18.1,95%CI 1.7–193.4).Conclusions SARS-CoV-2 seroprevalence in children has remarkably increased during the time of our *** was the only COVID-19-compatible symptom that was more frequent in seropositive than in seronegative children.

关键词： Antibodies Child COVID-19 Seroepidemiologic studies

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