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Non-exhaustive double effort test is reliable and estimates the firs ventilatory threshold intensity in running exercise

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Journal of Sport and Health Science 2018年第2期7卷 197-203页

作者： Lucas Dantas Maia Forte Fúlvia barros Manchado-Gobatto Roberta Cunha Matheus Rodrigues maria Ceclia Gallani Claudio Alexandre Gobatto School of Applied Science University of Campinas School of Nursing University of Campinas Faculty of Nursing Laval University

Purpose: The present study aimed to investigate the reliability of the non-exhaustive double effort(NEDE) test in running exercise and its associations with the ventilatory thresholds(VT_1 and VT_2) and the maximal lactate steady state(MLSS).Methods: Ten healthy male adults(age: 23 ± 4 years, height: 176.6 ± 6.4 cm, body mass: 76.6 ± 10.7 kg) performed 4 procedures:(1) a ramp test for VT_1 and VT_2 determinations measured by ratio of expired ventilation to O_2 uptake(VE/VO_2) and expired ventilation to CO_2 output(VE/VCO_2) equivalents, respectively;(2) the NEDE test measured by blood lactate concentration(NEDE_(LAC)) and heart rate responses(NEDE_(HR));(3) a retest of NEDE for reliability analysis; and(4) continuous efforts to determine the MLSS intensity. The NEDE test consisted of4 sessions at different running intensities. Each session was characterized by double efforts at the same running velocity(E1 and E2, 180 s), separated by a passive recovery period(90 s rest). LAC and HR values after E1 and E2(in 4 sessions) were used to estimate the intensity equivalent to"null delta" by linear fit. This parameter represents, theoretically, the intensity equivalent to maximal aerobic ***: The intraclass correlation coefficient indicated significant reliability for NEDE_(LAC)(0.93) and NEDE_(HR)(0.79)(both p < 0.05). There were significant correlations, no differences, and strong agreement with the intensities predicted by NEDE_(LAC)(10.1 ± 1.9 km/h) and NEDE_(HR)(9.8 ± 2.0 km/h) to VT_1(10.2 ± 1.1 km/h). In addition, despite significantly lower MLSS intensity(12.2 ± 1.2 km/h), NEDE_(LAC) and NEDE_(HR) intensities were highly correlated with this parameter(0.90 and 0.88, respectively).Conclusion: The NEDE test applied to running exercise is reliable and estimates the VT_1 intensity. Additionally, NEDE intensities were lower but still correlated with VT_2 and MLSS.

关键词： Aerobic exercise Anaerobic threshold Maximal lactate steady state Non-exhaustive protocol Training prescription Ventilatory threshold

Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin,TGF-β1,IL-13 and oxidative stress

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World Journal of Gastroenterology 2017年第28期23卷 5146-5157页

作者： Veruska Cintia Alexandrino de Souza Thiago Almeida Pereira Valéria Wanderley Teixeira Helotonio Carvalho maria Carolina Accioly Brelaz de Castro Carolline Guimaraes D’assuncao Andreia Ferreira de barros Camila Lima Carvalho Virgínia maria barros de Lorena Vláudia maria Assis Costa Alvaro Aguiar Coelho Teixeira Regina Celia Bressan Queiroz Figueiredo Sheilla Andrade de Oliveira Centro de Pesquisas Aggeu Magalhaes Fundacao Oswaldo Cruz Immunopathogenesis Section Laboratory of Parasitic DiseasesNational Institute of Allergy and Infectious DiseaseNational Institutes of HealthBethesdaMDUSA and Laboratório de Doencas Parasitárias e infecciosasFaculdade de MedicinaUniversidade Federal de Minas Gerais Departamento de Morfologia e Fisiologia Animal Universidade Federal Rural de Pernambuco Departamento de Biofísica e Radiobiologia Universidade Federal de Pernambuco Centro Acadêmico de Vitória Universidade Federal de Pernambuco Departamento de Medicina Tropical Universidade Federal de Pernambuco

To evaluate the therapeutic effects of bone marrow-derived CD11b+CD14+ monocytes in a murine model of chronic liver *** liver damage was induced in C57BL/6 mice by administration of carbon tetrachloride and ethanol for 6 mo. Bone marrow-derived monocytes isolated by immunomagnetic separation were used for therapy. The cell transplantation effects were evaluated by morphometry, biochemical assessment, immunohistochemistry and enzyme-linked immunosorbent ***11b+CD14+ monocyte therapy significantly reduced liver fibrosis and increased hepatic glutathione levels. Levels of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6 and IL-1β, in addition to pro-fibrotic factors, such as IL-13, transforming growth factor-β1 and tissue inhibitor of metalloproteinase-1 also decreased, while IL-10 and matrix metalloproteinase-9 increased in the monocyte-treated group. CD11b+CD14+ monocyte transplantation caused significant changes in the hepatic expression of α-smooth muscle actin and *** therapy is capable of bringing about improvement of liver fibrosis by reducing oxidative stress and inflammation, as well as increasing anti-fibrogenic factors.

关键词： Monocytes Bone marrow mononuclear cells Cell therapy Macrophages Glutathione Liver fibrosis

Phytochemical bioprospecting, antioxidant, antimicrobial and cytotoxicity activities of saline extract from Tithonia diversifolia(Hemsl) *** leaves

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Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 245-253页

作者： Bruno Rafael Barboza Bárbara Rafaela da Silva barros Bárbara de Azevedo Ramos Maiara Celine de Moura Thiago Henrique Napoleao maria Tereza dos Santos Correia Luana Cassandra Breitenbach barroso Coelho Iranildo José da Cruz Filho Ana maria Souto Maior Túlio Diego da Silva Leylianne de Cássia Rodrigues Nerys Edson Renan barros de Santana Cláudia Sampaio de Andrade Lima Virgínia maria barros de Lorena Cristiane Moutinho Lagos de Melo Department of Antibiotics Biosciences Centre Federal University of Pernambuco Department of Biochemistry Biosciences Centre Federal University of Pernambuco Center for Strategic Techmologies of the Northeast Department of Biophysics Biosciences Centre Federal University of Pernambuco Aggeu Magalhaes Institute IAM/FIOCRUZ

Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal ***:The saline extract was prepared,with NaCl(0.15 M),by constant stirring of the dried and pulverized leaves,followed by volume reduction by *** extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was *** antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice ***:Fifteen compounds were identified belonging to two main classes terpenoids and *** extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of ***,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these ***:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.

关键词： Tithonia diversifolia Extract Antioxidant Antifungal properties Immunostimulation

What a few hairs can tell us about the resource use of giant armadillos

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Integrative Zoology 2023年第1期18卷 129-142页

作者： Marcelo MAGIOLI Nina ATTIAS Gabriel MASSOCATO Danilo KLUYBER Marcelo Zacharias MOREIRA Katia maria Paschoaletto Micchi de barros FERRAZ Adriano Garcia CHIARELLO Arnaud L.J.DESBIEZ Instituto Pró-Carnívoros AtibaiaSão PauloBrazil Centro Nacional de Pesquisa e Conservação de Mamíferos Carnívoros(CENAP) Instituto Chico Mendes de Conservação da Biodiversidade(ICMBio)AtibaiaSão PauloBrazil Instituto de Conservação de Animais Silvestres(ICAS) Campo GrandeMato Grosso do SulBrazil Instituto de Pesquisas Ecológicas Organização Não GovernamentalNazaréPaulistaSão PauloBrazil Houston Zoo HoustonTexasUSA Naples Zoo at Caribbean Gardens NaplesFloridaUSA Laboratório de Ecologia Isotópica Centro de Energia Nuclear na AgriculturaUniversidade de São PauloPiracicabaSão PauloBrazil Laboratório de Ecologia Manejo e Conservação de Fauna Silvestre(LEMaC)Departamento de Ciências FlorestaisEscola Superior de Agricultura“Luiz de Queiroz”(ESALQ)Universidade de São Paulo(USP)PiracicabaSão PauloBrazil Departamento de Biologia Faculdade de FilosofiaCiências e LetrasUniversidade de São Paulo(USP)Ribeirão PretoSão PauloBrazil RZSS-Royal Zoological Society of Scotland(RZSS) MurrayfieldEdinburghUK

Understanding of resource requirements of vulnerable species is key for conservation ***,we used sta-ble carbon(δ13C)and nitrogen isotopes(δ15N)of hair from giant armadillos(Priodontes maximus)to understand individual resource *** collected hair from 19 giant armadillos analyzing 34 samples from the Brazilian Pan-tanal and compared the resource use between sexes and age *** also assessed the relationship of isotopic values with individuals’body mass and the habitat type *** observed a wide variation in resource use by giant armadillos,showing that individuals consume prey that feeds on distinct resources and occupies different habitat types,indicating that there is individual variation in foraging *** giant armadillos presented a mixed diet(C3/C4 resources,forests/open areas),but a quarter of individuals had strictly C4 diets(open areas).Males are more prone to forage on C4 resources,presenting an isotopic niche 2.6-times larger than that of *** presented diets more associated with habitats with high arboreal cover(C3 resources,forests),while adults foraged more in open areas(C4 resources).This result is mirrored by the positive relationship betweenδ13C values and body mass,suggesting that larger giant armadillos tend to feed more in open *** observed thatδ13C values decreased as individuals increased the use of habitats with high arboreal *** stress the importance of con-serving the natural mosaic in the Pantanal landscape for the species persistence,given that giant armadillos require all habitat types to fulﬁll their dietary,spatial,and developmental needs.

关键词： diet ontogenetic changes resource partitioning stable isotopes telemetry

Understanding the corrosion of Mg alloys in in vitro urinary tract conditions: A step forward towards a biodegradable metallic ureteral stent

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Journal of Magnesium and Alloys 2023年第11期11卷 4301-4324页

作者： Margarida Pacheco Ivo M.Aroso Joana M.Silva Sviatlana V.Lamaka Jan Bohlen maria Nienaber Dietmar Letzig Estêvão Lima Alexandre A.barros Rui L.Reis 3B’s Research Group-Research Institute on Biomaterials Biodegradables and BiomimeticsUniversity of MinhoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimarãesPortugal Institute of Surface Science Helmholtz-Zentrum hereon GmbHGermany Institute of Material and Process Design Helmholtz-Zentrum hereon GmbHGermany School of Health Sciences Life and Health Sciences Research Institute(ICVS)University of MinhoBragaPortugal

Ureteral stents play a fundamental role in modern time urology. However, following the deployment, stent-related symptoms are frequent and affect patient health and quality of life. Using biodegradable metals as ureteral stent materials have emerged as a promising strategy, mainly due to the improved radial force and slower degradation rate expected. Therefore, this study aimed to characterize different biodegradable metals in urinary tract environment to understand their propensity for future utilization as base materials for ureteral stents. The corrosion of 5 Mg alloys - AZ31, Mg-1Zn, Mg-1Y, pure Mg, and Mg-4Ag - under simulated urinary tract conditions was accessed. The corrosion layer of the different alloys presented common elements, such as Mg(OH)_(2), MgO, and phosphate-containing products, but slight variations in their chemical compositions were detected. The corrosion rate of the different metals varied, which was expected given the differences in the corrosion layers. On top of this, the findings of this study highlighted the significant differences in the samples' corrosion and corrosion layers when in stagnant and flowing conditions. With the results of this study, we concluded that Mg-1Zn and Mg-4Ag presented a higher propensity for localized corrosion, probably due to a less protective corrosion layer;Mg-4Ag corroded faster than all the other four alloys,and Mg-1Y stood out due to its distinct corrosion pattern, that showed to be more homogeneous than all the other four samples, making this one more attractive for the future studies on biodegradable metals.

关键词： Biodegradable metals Magnesium alloys Localized corrosion Biodegradable metallic ureteral stent

Classification of Abandoned Areas for Solar Energy Projects Using Artificial Intelligence and Quantum Mechanics

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Journal of Geographic Information System 2021年第3期13卷 318-339页

作者： David Gabriel de barros Franco maria Teresinha Arns Steiner Undergraduate Program in Logistics Universidade Federal do Tocantins (UFT) Araguaína Brasil Graduate Program in Industrial Engineering and Systems Pontifícia Universidade Católica do Paraná (PUC-PR) Curitiba Brasil

The increasing demand for energy has intensified recently, requiring alternative sources to fossil fuels, which have become economically and environmentally unfeasible. On the other hand, the increasing land occupation in recent centuries is a growing problem, demanding greater efficiency, particularly in the reuse of abandoned areas, which has become an alternative. An interesting alternative would be installing energy facilities like solar, wind, biomass, and geothermal, in these areas. The objective of this paper is to develop a classification methodology, based on Artificial Intelligence (AI) and Quantum Theory (QT), to automatically carry out the classification of abandoned areas suitable for the settlement of these power plants. Artificial Neural Networks (ANNs) improved by the hybrid algorithm Quantum-behaved Particle Swarm Optimization (QPSO) together with the Levenberg-Marquardt Algorithm (LMA) were used for the classification task. In terms of Mean Squared Error (MSE), the QPSO-LMA approach achieved a decrease of 19.6% in relation to the classical LMA training with random initial weights. Moreover, the model’s accuracy showed an increase of 7.3% for the QPSO-LMA over the LMA. To validate this new approach, it was also tested on six different datasets available in the UCI Machine Learning Repository and seven classical techniques established in the literature. For the problem of installing photovoltaic plants in abandoned areas, the knowledge acquired with the solar dataset can be extrapolated to other regions.

关键词： Solar Energy Soil Reuse Artificial Neural Networks Quantum-Behaved Particle Swarm Optimization

Sediment transport under the presence and absence of emergent vegetation in a natural alluvial channel from Brazil

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International Journal of Sediment Research 2016年第4期31卷 360-367页

作者： Yuri Jacques Agra Bezerra da Silva José Ramon barros Cantalice Vijay P.Singh Cinthia maria Cordeiro Atanázio Cruz Wagner Luís da Silva Souza Soil Conservation Engineering Laboratory Rural Federal University of Pernambuco(UFRPE) Brazil Department of Biological and Agricultural Engineering Texas A & M University College Station

Studies regarding the influence of emergent vegetation on sediment transport are scarce and have mainly focused on flume *** fill this gap and also meet the international need,we aimed to evaluate the influence of emergent vegetation(Echinodorus macrophyllus) on sediment transport of Capibaribe River,*** and suspended sediment measurements were carried out using the US BLH 84 and US DH 48 samplers,*** of stem diameter,stem spacing and plant density were performed in conjunction with flow and sediment field *** on our results,0.45 m swas the threshold of mean flow velocity supported by *** under field *** value can be helpful for other rivers with gravel-bed river to armoured layer ratio(AR=D)/D=12.50)-natural conditions observed in Capibaribe River-or where the vegetation can provide positive effects,such as increase the bed stability,assist water restoration/rehabilitation and decrease water *** results can hopefully be used in engineering practice and ecosystem *** general,both the drag coefficient and drag force varied inversely and directly with the mean flow velocity and vegetation density,*** vegetation resistance force was inversely proportional to the bedload transport owing to the resistance caused by emergent *** finding was supported by the clear decoupling between nonvegetated and emergent vegetated conditions indicated by cluster *** study results provided a reasonable understanding of the interaction between emergent vegetation,water flow and sediment transport in the Capibaribe River.

关键词： Vegetation drag force Vegetation drag coefficient Plant Reynolds number Hierarchical cluster analysis and principal component analysis

Semi-Quantitative PCR for Quantification of Hepatotoxic Cyanobacteria

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Journal of Environmental Protection 2012年第5期3卷 426-430页

作者： Selma Gouvea-barros maria do Carmo Bittencourt-Oliveira Institute of Biological Sciences Sao Paulo State UniversityRio ClaroBrazil Department of Biological Sciences Luiz de Queiroz College of AgricultureUniversity of Sao PauloPiracicabaBrazil Departamento de Gestao Ambiental Instituto Federal de EducacaoCiencia e Tecnologia do Sul de Minas Gerais—Campus InconfidentesInconfidentesBrazil

Blooms of microcystin-producing cyanobacteria are a problem worldwide. Microcystin is a liver hepatotoxin commonly found in bodies of water and is produced mainly by the genus Microcystis. The aim of the present study was to develop and assess a competitive PCR method for the quantification of toxic and non-toxic Microcystis cells using the cpcBA and mcyB genes, which are respectively involved in the formation of phycocyanin and biosynthesis of microcystin. For the acquisition of competitor DNA, amplification sequences were carried out of the “cell DNA equivalent” of microcystin-producing (BCCUSP18) and non-microcystin-producing (BCCUSP03) strains of Microcystis spp. using primers described in the literature as well as others designed for the present study. The method was successfully developed, as competitor DNA was constructed and co-amplified with the target DNA. Competitive PCR proved to be useful in quantifying toxic and non-toxic cells of Microcystis spp. strains, representing a helpful methodology tool to study isolated toxin-producing cyanobacteria.

关键词： Cyanobacteria Microcystis Competitive PCR mcyB cpcBA

The Various Description of Biodiversity in Compulsory Education Textbooks

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Journal of Life Sciences 2011年第10期5卷 856-862页

作者： maria-Jesus Fuentes Silveira Susana Garcia barros Cristina Martinez Losada IES Monte Castelo （High School） Burela 27880 Spain Department of Pedagogy and Didactic University of A Coruha 4 Coruna 15071 Spain

We intend to establish how species diversity is dealt with, within the study of the ecosystem, in books for the 4th year of Compulsory Secondary Education, ESO （15-16 year-old students）. The analysis was carried out using a key issues dossier based on school science, which not only provides a description of reality, but also introduces a theoretical explanatory framework. Regarding species diversity, we focused on the descriptive approach, considering the link between diversity and trophic relationships, ecological succession and ecosystem stability through change, consequences for biodiversity of human activities and mechanisms to face such consequences; and on the explanatory perspective, which provides an interpretative answer for all the aspects previously mentioned. The findings drawn from textbooks show that biodiversity is linked both to the study of the ecosystem and to the impact of human societies on its maintenance. However, such study is particularly focused on the descriptive, rather than on the explanatory level. So, teachers should improve the study of biodiversity foresting a balance between descriptive and explanatory level.

关键词： Biodiversity textbooks secondary education human activity ecosystem.