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Comparison of Blood Group Molecular Genotyping to Traditional Serological Phenotyping in Patients with Chronic or Recent Blood Transfusion

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Journal of Biosciences and Medicines 2016年第3期4卷 1-8页

作者： Zhan Ye Daoping Zhang Leonard Boral counts liz John May Department of Pathology and Laboratory Medicine University of Kentucky Lexington KY USA Kentucky Blood Center 3121 Beaumont Centre Circle Lexington KY USA

Objectives: Accurately identifying the Antigens (Ags) on recipient red blood cells (RBCs) is critical in prevention of RBC alloimmunization in chronically transfused patients. The goal of this study was to compare RBC molecular genotyping to serological phenotyping in those patients. Methods: Serological phenotyping and molecular genotyping methods were used to study blood samples from 18 healthy blood donors and 16 transfused patients. Reticulocyte harvesting or hypotonic cell separation was added to recheck RBC phenotypes of the patients with discrepancies between phenotyping and genotyping. Results: No discrepancies were found between the two genotyping methods in all the donors and patients. 1 of 9 sickle-cell disease (SCD) patients and all 3 thalassemia patients demonstrated discrepancies in multiple blood groups between phenotyping and genotyping, which were not corrected by reticulocyte harvesting or hypotonic cell separation. Conclusions: These findings suggest that RBC molecular genotyping is superior to serological phenotyping in chronically transfused SCD or thalassemia patients.

关键词： Blood Group Molecular Genotyping Serological Phenotyping Chronic Blood Transfusion

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Litopenaeus vannamei immunestimulated with Macrocystis pyrifera extract:improving the immune response against Vibrio campbellii

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Journal of Coastal Life Medicine 2014年第8期 617-624页

作者： Liliana Noemi Sánchez Campos Fernando Díaz Herrera Ana Denisse Re Araujo Ricardo Alberto Gonzalez Sánchez Marcial Leonardo lizárraga Partida Manuel de Jesús Acosta Ruiz Alexei Fedorovish Licea Navarro Molecular Immunology Laboratory Marine Biotechnology DepartmentCenter for Scientific Research and Higher Education of Ensenada(CICESE)3918 Ensenada Tijuana Road Ensenada Baja CaliforniaMexico Ecophysiology of Aquatic Organisms Laboratory Marine Biotechnology DepartmentCenter of Scientific Research and High Education of Ensenada Baja California3918 Ensenada Tijuana Road Ensenada Baja CaliforniaMexico Molecular Ecology Laboratory Marine Biotechnology DepartmentCenter of Scientific Research and High Education of Ensenada Baja California3918 Ensenada Tijuana Road Ensenada Baja CaliforniaMexico Functional Nutrition Laboratory Marine Biotechnology DepartmentCenter of Scientific Research and High Education of Ensenada Baja California3918 Ensenada Tijuana Road Ensenada Baja CaliforniaMexico

Objective:To examine the immune responses of Litopenaeus vannamei after different treatments with a hot water extract of Macrocystis pyrifera(***)and a subsequent challenge with Vibrio campbellii(***).Methods:A total of 184 adult white shrimp that were infected with ***(1×10^(6) CFU/shrimp)were immunostimulate by the hot-water extract from *** via either injection(10μg)or immersion(350 mg/L),the experimental controls were injected with either saline solution or ***(1×10^(6) CFU/shrimp).The bacterial DNA depuration rate,antimicrobial activity and total hemocyte count were evaluated in hemolymph samples at 2,6,12,24,48 and 72 h ***:Injected shrimp(10μg *** extract)demonstrated the best clearance of bacterial infection,with 82%survival at 72 h post-infection(cellular response).Hemolymph from the immersed organisms had the best antimicrobial activity against Escherichia coli growth;specifically,the most efficient antimicrobial activity was observed at 24 h *** types of immunostimulated shrimp had similar total hemocyte counts at 24 h post-infection(1.63-1.59 million/mL);however,after 72 h,injected shrimp had higher total hemocyte counts than immersed animals(2.59 v.s.0.56 million/mL).Conclusions:The injection of the *** hot-water extract facilitated a more efficient response to *** infection due to the stimulation of the hemocytes of the *** other words,the cellular immune response was more efficient to eliminate bacterial infection than the humoral response in shrimp.

关键词： Immune stimulation Hot-water extract Clearance rate Antimicrobial activity Seaweed Vibrio campbellii Litopenaeus vannamei Macrocystis pyrifera PCR

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