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Expression of L amino acid transport system 1 and analysis of iodine-123-methyltyrosine tumor uptake in a pancreatic xenotransplantation model using fused high-resolution-micro-SPECT-MRI

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Hepatobiliary & Pancreatic Diseases International 2011年第1期10卷 30-37页

作者： corinna von forstner Maaz Zuhayra Ole Ammerpohl Sanjay Tiwari Olav Jansen Holger Kalthoff Eberhard Henze Jan-Hendrik Egberts Department of Nuclear Medicine University Hospital of Schleswig-Holstein Institute for Experimental Cancer Research University Hospital of Schleswig-Holstein Institute of Neuroradiology University Hospital of Schleswig-Holstein Department of General Surgery and Thoracic Surgery University Hospital of Schleswig-Holstein

BACKGROUND:The specificity in discriminating pancreatitis is limited in the positron emission tomography(PET)using ***,PET is not widely available compared to the single photon emission computed tomography(SPECT).Since amino acids play a minor role in metabolism of inflammatory cells,the potential of the SPECT tracer,3-[ 123 I]iodo-L-α-methyltyrosine(123I-IMT),for detecting pancreatic cancer was examined in xenotransplantation models of human pancreatic carcinoma in mice. METHODS: 123 I-IMT was injected to eight mice inoculated with subcutaneous or orthotopic pancreatic *** high-resolution-micro-SPECT(Hi-SPECT)and magnetic resonance imaging were *** gene expression level of L amino acid transport-system 1(LAT1)was analyzed and correlated with tumor uptake of 123 I-IMT. RESULTS:A high uptake of 123 I-IMT was detected in all tumor-bearing *** median tumor-to-background ratio (T/B)was 12.1(2.0-13.2)for orthotopic and 8.4(1.8-11.1)for subcutaneous xenotransplantation,***, the LAT1 expression in transplanted Colo357 cells was increased compared to non-malignant ***:Our mouse model could show a high 123 I-IMT uptake in pancreatic *** MRI scans facilitate precise evaluation of uptake in the specific regions of *** studies are required to confirm these findings in tumors derived from other human pancreatic cancer *** amino acids play a minor role in the metabolism of inflammatory cells,the potential for application of 123 I-IMT to distinguish pancreatic tumor from inflammatory pancreatitis warrants further investigation.

关键词： pancreatic ductal adenocarcinoma iodine-123-methyltyrosine high-resolution-micro-SPECT L amino acid transport-system 1 xenotransplantation model

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Repression of the Arabidopsis thaliana Jasmonic Acid/Ethylene-Induced Defense Pathway by TGA-Interacting Glutaredoxins Depends on Their C-Terminal ALWL Motif

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Molecular Plant 2012年第4期5卷 831-840页

作者： Mark Zander Shuxia Chen Julia Imkampe corinna Thurow Christiane Gatz Albrecht-von-Hailer-Institute for Plant Sciences Georg-August-University Gottingen Julia-Lermontowa-Weg 3 D-37077 Gottingen Germany College of Horticulture Northwest A&F University Taicheng Road 3 Yangling Shaanxi 712100 PR China

Glutaredoxins are small heat-stable oxidoreductases that transfer electrons from glutathione （GSH） to oxi- dized cysteine residues, thereby contributing to protein integrity and regulation. In Arabidopsis thaliana, floral glutare- doxins ROXY1 and ROXY2 and pathogen-induced ROXY19/GRX480 interact with bZIP transcription factors of the TGACG （TGA） motif-binding family. ROXY1, ROXY2, and TGA factors PERIANTHIA, TGA9, and TGA10 play essential roles in floral development. In contrast, ectopically expressed ROXY19/GRX480 negatively regulates expression of jasmonic acid （JA）/ ethylene （ET）-induced defense genes through an unknown mechanism that requires clade II transcription factors TGA2, TGA5, and/or TGA6. Here, we report that at least 17 of the 21 land plant-specific glutaredoxins encoded in the Arabidopsis genome interact with TGA2 in a yeast-two-hybrid system. To investigate their capacity to interfere with the expression of JA/ET-induced genes, we developed a transient expression system. Activation of the ORA59 （OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF-domain protein 59） promoter by transcription factor EIN3 （ETHYLENE INSENSITVE 3） was sup- pressed by co-expressed ROXY19/GRX480. Suppression depended on the L＊＊LL motif in the C-terminus of ROXY19/ GRX480. This putative protein interaction domain was recently described as being essential for the TGA/ROXY interaction. Ten of the 17 tested ROXY proteins suppressed ORA59 promoter activity, which correlated with the presence of the C-terminal ALWL motif, which is essential for ROXY1 function in flower development. ROXY19/GRX480-mediated repres- sion depended on the GSH binding site, suggesting that redox modification of either TGA factors or as yet unknown target proteins is important for the suppression of ORA59 promoter activity.

关键词： Arabidopsis thaliana cross-talk defense ethylene jasmonic acid glutaredoxins salicylic acid.

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Effect of biochar fertilizers on amino acid variability of Secale cereale and Lupinus angustifolius

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Biochar 2019年第2期1卷 187-201页

作者： Katja Wiedner corinna Schimpf Steven Polifka Bruno Glaser Soil Biogeochemistry Institute of Agronomy and Nutritional SciencesMartin Luther University Halle-WittenbergVon-Seckendorff-Platz 306120 Halle(Saale)Germany

Little is known on the effects of biochar on N uptake and amino acid variability in crops such as winter rye and narrow-leafed lupine despite the fact that amino acids are important indicators,for food quality and plant stress.N uptake of both crops showed contrasting results when treated with different biochar *** amino acid contents referred to total nitro-gen generally tend to decrease in rye grains in the presence of biochar;whereas lupine seeds were more or less unaffected by biochar combined with mineral fertilizer or *** lupine seeds,total amino acid contents significantly increased when biochar was mixed with digestate but decreased when mixed with fermented ***,one of the most limiting amino acids in cereals,reached the recommended value of 4 g kg^(−1) in rye grain for most biochar *** lupine seeds,lysine decreased when biochar had been applied but were still in the recommended range when used as animal ***,an indicator for plant stress,significantly decreased(−49%)in rye when 2 Mg biochar ha^(−1) was added in combination with mineral *** contrast,proline increased when biochar was added to organic(digestate and compost)fertilizers(up to 43%).Further biochar research should focus much more on food quality,which is a key challenge for global food production.

关键词： Food quality Protein Lupine Winter rye Yield

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BAG6 restricts pancreatic cancer progression by suppressing the release of IL33-presenting extracellular vesicles and the activation of mast cells

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Cellular & Molecular Immunology 2024年第8期21卷 918-931页

作者： Bilal Alashkar Alhamwe Viviane Ponath Fahd Alhamdan Bastian Dörsam Clara Landwehr Manuel Linder Kim Pauck Sarah Miethe Holger Garn Florian Finkernagel Anna Brichkina Matthias Lauth Dinesh Kumar Tiwari Malte Buchholz Daniel Bachurski Sabrina Elmshäuser Andrea Nist Thorsten Stiewe Lisa Pogge von Strandmann Witold Szymański Vanessa Beutgen Johannes Graumann Julia Teply-Szymanski corinna Keber Carsten Denkert Ralf Jacob Christian Preußer Elke Pogge von Strandmann Institute for Tumor Immunology Philipps-University35043MarburgGermany Core Facility Extracellular Vesicles Philipps-University35043MarburgGermany Department of Anesthesiology Critical Careand Pain MedicineCardiac Anesthesia DivisionBoston Children’s HospitalBostonUSA Department of Immunology and Anaesthesia Harvard Medical SchoolBostonMAUSA Translational Inflammation Research Division&Core Facility for Single Cell Multiomics Philipps-University35043MarburgGermany Core Facility Bioinformatics Philipps-University35043MarburgGermany Clinic for Gastroenterology Endocrinology and MetabolismCenter for Tumor and Immune BiologyPhilipps-University35043MarburgGermany Institute of Systems Immunology Philipps-University35043MarburgGermany Cluster of Excellence on Cellular Stress Responses in Aging-Associated Diseases(CECAD) University of CologneCologneGermany Institute of Molecular Oncology and Genomics Core Facility Member of the German Center for Lung Research(DZL)Philipps-University35043MarburgGermany Institute of Lung Health Justus Liebig University35392GiessenGermany Institute of Translational Proteomics&Core Facility Translational Proteomics Biochemical/Pharmacological CentrePhilipps-University35043MarburgGermany Institute of Pathology Philipps-University Marburg and University Hospital Marburg(UKGM)MarburgGermany Department of Cell Biology and Cell Pathology Philipps-University35043MarburgGermany

Recent studies reveal a critical role of tumor cell-released extracellular vesicles(EVs)in pancreatic cancer(PC)***,driver genes that direct EV function,the EV-recipient cells,and their cellular response to EV uptake remain to be ***,we studied the role of Bcl-2-associated-anthanogene 6(BAG6),a regulator of EV biogenesis for cancer *** used a Cre recombinase/LoxP-based reporter system in combination with single-cell RNA sequencing to monitor in vivo EV uptake and tumor microenvironment(TME)changes in mouse models for pancreatic ductal adenocarcinoma(PDAC)in a Bag6 pro-or deficient *** vivo data were validated using mouse and human organoids and patient *** data demonstrated that Bag6-deficient subcutaneous and orthotopic PDAC tumors accelerated tumor growth dependent on EV ***,this was attributed to mast cell(MC)activation via EV-associated *** MCs promoted tumor cell proliferation and altered the composition of the TME affecting fibroblast polarization and immune cell *** cell proliferation and fibroblast polarization were mediated via the MC secretome containing high levels of PDGF and *** with high BAG6 gene expression and high protein plasma level have a longer overall survival indicating clinical *** current study revealed a so far unknown tumor-suppressing activity of BAG6 in ***6-deficiency allowed the release of EV-associated IL33 which modulate the TME via MC activation promoting aggressive tumor *** depletion using imatinib diminished tumor growth providing a scientific rationale to consider imatinib for patients stratified with low BAG6 expression and high MC infiltration.

关键词： Pancreatic cancer EVs BAG6 Mast cells

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