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Detection of Mycoplasmas in Patients with Amyotrophic Lateral Sclerosis

Advances in Microbiology 2014年第11期4卷 712-719页

作者： constantino gil Alma Aurora Sánchez González Isidro Lecona León Antonio Rivera Rayo Santellán Olea Lilia Cedillo Centro de Investigaciones en Ciencias Microbiológicas Instituto de Ciencias Benemérita Universidad Autónoma de Puebla Edificio 103J Ciudad Universitaria Col. San Manuel Puebla Pue México Escuela de Biología Benemérita Universidad Autónoma de Puebla Edificio 112A Ciudad Universitaria Col. San Manuel Puebla Pue México Servicio de Neurología Centro Médico Nacional Manuel ávila Camacho IMSS Puebla México

Amyotrophic Lateral Sclerosis (ALS) is a progressive degenerative disease of the motor neurons and the cause is unknown. Diverse factors such as genetic defects, nutritional deficiencies, head trauma, environmental toxin, autoimmune responses and viral and bacterial infections are involved. Mycoplasmas have been implicated as causal agents of different illnesses in human. The purpose of this study was to investigate the presence of mycoplasmas in the bloodstream of patients with ALS. Patients with ALS and healthy individuals were included in the study. A blood sample was taken in tubes with or without anticoagulant. Mycoplasmas were detected by culture or direct PCR, and the presence of antibodies IgM and IgG against LAMPs of these microorganisms by Western blot. Cultures for aerobic facultative bacteria were also done. Blood samples from 13 patients and 44 healthy individuals were screened. All blood cultures for non-fermentative mycoplasmas and aerobic facultative bacteria were negative. Cultures for fermentative mycoplasmas were considered positive after identification of mycoplasmal DNA by PCR. Mycoplasma sp. was detected by culture or direct PCR in 6/13 (46%) patients and in 4/44 (9%) of healthy individuals. M. fermentans was detected by PCR using specific primers in six patients and in two healthy individuals. IgM against LAMPs of M. fermentans were detected in 6/13 (46%) blood samples from patients and in 13/44 (30%) from healthy individuals, while. IgG was detected in 4/13 (31%) patients and in 3/44 (7%) healthy individuals. The results of this study show that mycoplasmas cause a systemic infection and could play a role in the origin or progression of the ALS.

关键词： Amyotrophic Lateral Sclerosis Mycoplasmas Immune Response

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Presence of Mycoplasma *** Patients with Asthma or Allergic Rhinitis

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Advances in Microbiology 2014年第11期4卷 720-725页

作者： Munoz-Zurita Guillermo Paz Martínez David Yánez Santos Jorge Antonio gil Juárez constantino Cedillo Ramírez María Lilia Alumno de Doctorado del Posgrado en Ciencias Ambientales Benemérita Universidad Autónoma de PueblaPueblaMexico Hospital Universitario de Puebla Benemérita Universidad Autónoma de PueblaPueblaMexico Facultad de Estomatología Benemérita Universidad Autónoma de PueblaPueblaMexico Centro de Investigaciones en Ciencias Microbiológicas Benemérita Universidad Autónoma de PueblaPueblaMexico Centro de Detección Biomolecular VIEPBenemérita Universidad Autónoma de PueblaPueblaMexico

Objective: To compare the presence of Mycoplasma spp. in pharyngeal swabs of patients with asthma, allergic rhinitis and controls, also to compare the reactivity to allergens in the three groups. Material and Methods: Throat swabs of 120 patients with asthma, 211 allergic rhinitis patients and 171 healthy persons were cultured in E media. Positive cultures were identified by PCR. Susceptibility of patients positive for isolation of Mycoplasma spp. to indoor, forest and undergrowth allergens were determined. Results: Mycoplasma spp. was isolated in 26.6% of samples from asthmatic patients, 36% in patients with allergic rhinitis and 2.9% in the group of healthy persons. Positive patients for Mycoplasma with asthma and allergic rhinitis showed susceptibility tomite, grass and thunder mainly. Conclusion: There is a higher incidence of Mycoplasma spp. in asthmatic and allergic rhinitis patients than healthy persons. Only patients with asthma or allergic rhinitis showed reactivity to environmental allergens.

关键词： Asthma Allergic Rhinitis Allergens Mycoplasma Spp.

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