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Injection of bone marrow mesenchymal stem cells by intravenous or intraperitoneal routes is a viable alternative to spinal cord injury treatment in mice

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Neural Regeneration Research 2018年第6期13卷 1046-1053页

作者： Bruna dos Santos Ramalho FernANDa Martins de Almeida conrado mendonca sales Silmara de Lima Ana Maria Blanco Martinez Laboratorio de Neurodegeneracao e Reparo Departamento de Patologia - Faculdade de Medicina HUCFF Universidade Federal do Rio de Janeiro Rio de laneiro Brazil

In spite of advances in surgical care and rehabilitation, the consequences of spinal cord injury （SCI） are still challenging. Several experimental therapeutic strategies have been studied in the SCI field, and recent advances have led to the development of therapies that may act on the inhibitory microenvironment. Assorted lineages of stem cells are considered a good treatment for SCI. This study investigated the effect of systemic transplantation of mesenchymal stem cells （MSCs） in a compressive SCI model. Here we present results of the intraperitoneal route, which has not been used previously for MSC administration after compressive SCI. We used adult female C57BL/6 mice that underwent laminectomy at the T9 level, followed by spinal cord compression for 1 minute with a 30-g vascular clip. The animals were divided into five groups： sham （anesthesia and laminectomy but without compression injury induction）, MSC i.p. （intraperitoneal injection of 8×10^5 MSCs in 500 μL of DMEM at 7 days after SCI）, MSC i.v. （intravenous injection of 8 × 10^5 MSCs in 500μL of DMEM at 7 days after SCI）, DMEM i.p. （intraperitoneal injection of 500μL of DMEM at 7 days after SCI）, DMEM i.v. （intravenous injection of 500 μL of DMEM at 7 days after SCI）. The effects of MSCs transplantation in white matter sparing were analyzed by luxol fast blue staining. The number of preserved fibers was counted in semithin sections stained with toluidine blue and the presence of trophic factors was analyzed by immunohistochemistry. In addition, we analyzed the locomotor performance with Basso Mouse Scale and Global Mobility Test. Our results showed white matter preservation and a larger number of preserved fibers in the MSC groups than in the DMEM groups. Furthermore, the MSC groups had higher levels of trophic factors （brain-derived neurotrophic factor, nerve growth factor, neurotrophin-3 and neurotrophin-4） in the spinal cord and improved locomotor performance. Our results indicate that injection of MSC

关键词： spinal cord injury mesenchymal stem cells intravenous route intraperitoneal route functional recovery systemic transplantation neural regeneration

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Effects of blood flow restriction without additional exercise on strength reductions and muscular atrophy following immobilization:A systematic review

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Journal of Sport and Health Science 2020年第2期9卷 152-159页

作者： Mikhail Santos Cerqueira Jose Diego sales Do Nascimento Daniel Germano Maciel Jean Artur mendonca Barboza Wouber Herickson De Brito Vieira Department of Physical Therapy Laboratory of Muscle PerformanceFederal University of Rio Grande do NorteNatalRio Grande do Norte 59072-970Brazil Department of Physical Therapy Neuromuscular Performance Analysis LaboratoryFederal University of Rio Grande do NorteNatalRio Grande do Norte 59072-970Brazil

Purpose:To investigate whether blood flow restriction(BFR) without concomitant exercise mitigated strength reduction and atrophy of thigh muscles in subjects under immobilization for lower ***:The following databases were searched:PubMed,CINAHL,PEDro,Web of Science,Central,and ***:The search identified 3 eligible studies,and the total sample in the identified studies consisted of 38 *** and isometric torque of the knee flexors and extensors was examined in 2 ***-sectional area of thigh muscles was evaluated in 1 study,and thigh girth was measured in 2 *** BFR protocol was 5 sets of 5 min of occlusion and 3 min of free flow,twice daily for approximately 2 *** a whole,the included studies indicate that BFR without exercise is able to minimize strength reduction and muscular atrophy after *** is crucial to emphasize,however,that the included studies showed a high risk of bias,especially regarding allocation concealment,blinding of outcome assessment,intention-to-treat analyses,and group similarity at ***:Although potentially useful,the high risk of bias presented by original stodies limits the indication of BFR without concomitant exercise as an effective countermeasure against strength reduction and atrophy mediated by immobilization.

关键词： Disuse Hypoxia Ischemia Muscle wasting Rehabilitation

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