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Expression, purification, and subcellular localization of phospholipase C in Dunaliella salina

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Journal of Oceanology and Limnology 2019年第4期37卷 1363-1371页

作者： cong Yuting WANG Yuan yue Jinrong XING Zhenyu GAO Xiangnan CHAI Xiaojie Key Laboratory of Hydrobiology in Liaoning Province College of Fisheries and Life Science Dalian Ocean University

Plants possess effective mechanisms to respond quickly to the external environment. Rapid activation of phosphatidylinositol-specific phospholipase C (PLC) enzymes occurs after a stimulus. The PLC in Dunaliella salina plays important roles in growth and stress responses. However, the molecular basis of PLC action in D. salina remains little understood. To gain insight into the potential biological functions of this enzyme, we cloned a phospholipase C gene from D. salina in a previous study, named DsPLC (GenBank No. KF573428). Here, we present the prokaryotic expression, purification, and characterization of the DsPLC gene. The entire coding region of DsPLC was inserted into an expression vector pET32a, and the DsPLC gene was successfully expressed in Escherichia coli. The DsPLC protein was purified and identified using a polyclonal antibody and western blotting. Expressing DsPLC fused with a green fluorescent protein (GFP) in onion showed that DsPLC-GFP was localized to the intracellular membrane. Quantitative real-time PCR analysis revealed that the relative expression of the DsPLC gene was induced significantly by 3.0-mol/L NaCl at 4 h. Our results support the importance of PLC enzymes in plant defense signaling. This study provides a basis for further functional studies of the DsPLC gene and for additional analysis of the potential roles of PLC enzymes in response to abiotic stress.

关键词： Dunaliella salina DsPLC gene prokaryotic expression subcellular localization salt stress

A Marcinkiewicz criterion for L^(p)-multipliers related to Schrodinger operators with constant magnetic fields

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Science China Mathematics 2015年第2期58卷 389-404页

作者： DENG Liu Rui MA bo Lin LIU Shao yue College of Economics and Management Hunan Normal University College of Mathematics and Information Engineering Jiaxing University Department of Mathematics Xiangtan University

In this paper,we follow Dappa’s work to establish the Marcinkiewicz criterion for the spectral multipliers related to the Schrdinger operator with a constant magnetic *** prove that if m and m′are locally absolute... 详细信息

关键词： magnetic Schrodinger operator spectral multiplier Riesz means

Mass segregation of embedded clusters in the Milky Way

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Research in Astronomy and Astrophysics 2013年第3期13卷 277-289页

作者： Xin-yue Er Zhi-bo Jiang Yan-Ning Fu Purple Mountain Observatory Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences

Embedded clusters are ideal laboratories for understanding the early phase of the dynamical evolution of clusters as well as massive star formation. An interesting observational phenomenon is that some of the embedded clusters show mass segregation, i.e., the most massive stars are preferentially found near the cluster center. We develop a new approach to describe mass segregation. Using this approach and the Two Micron All Sky Survey Point Source Catalog （2MASS PSC）, we analyze 18 embedded clusters in the Galaxy. We find that 11 of them are mass-segregated and that the others are not mass-segregated. No inversely mass-segregated cluster is found.

关键词： open clusters and associations: general -- stars: formation -- methods:data analysis

In situ prepared Al-Si alloy matrix composites reinforced by γ-Al_2O_3p

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Journal of University of Science and Technology Beijing 2007年第3期14卷 276-279页

作者： Lan Jiang bo Jiang Zhaofei Wang yue Wang Ji Liu School of Materials and Metallurgy Northeastern University Shenyang 110004 China School of Environmental and Biological Science ＆ Technology Dalian University of Technology Dalian 116024 China

A γ-Al2O3 particles reinforced Al-Si alloy matrix composite was fabricated by adding NH4Al（SO4）2 to molten aluminum alloy. TEM observation shows that in-situ γ-Al2O3 particles are generally spherical and uniformly distributed in the matrix. The results of dry sliding wear tests show that the wear resistance of the composites increases with increasing mass fraction, and the volume loss is considerably lesser than that of the matrix and is lesser than that of the composites by adding γ-Al2O3 particles directly.

关键词： aluminum matrix composite in-situ γ-Al2O3 particles microstructure wear behavior

Optimization of parental RNAi conditions for hunchback gene in Locusta migratoria manilensis （Meyen）

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Insect Science 2010年第1期17卷 1-6页

作者： Zheng-bo He yue-Qing Cao Yu-Xian Xia Genetic Engineering Research Center College of Bioengineering Chongqing University Chongqing Engineering Research Center for Fungal Insecticides and Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission Chongqing China

An investigation on the optimization of parental RNA interference （RNAi） conditions for hunchback （hb） gene in Locusta migratoria manilensis （Meyen） was conducted. Double stranded RNA （dsRNA） corresponding to hb gene was injected into haemocoel of female adults ofL. migratoria manilensis. Embryos developed from the eggs laid by the injected adults on the 7th day after eclosion showed observable effects of RNAi for hb. The silencing effect after delivery treatment of dsRNA for hb gene was maintained for more than 21 days. A significant decrease of hb transcripts was further confirmed by Northern blot analysis. The dose of dsRNA/insect at 2μg could trigger 96.9% RNAi effects, while silencing appeared to have no dependence on the size of dsRNA. Results suggest that parental RNAi could be employed to efficiently identify the developmental gene functions in L. migratoria manilensis.

关键词： hunchback Locusta migratoria manilensis parental RNA interference

Effect of fluidity on the manufacturing of open cell magnesium alloy foams

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Journal of Magnesium and Alloys 2016年第1期4卷 1-7页

作者： Xue-Zheng yue Koichi Kitazono Xue-Jie yue bo-Young Hur Graduate School of System Design Tokyo Metropolitan UniversityTokyo 191-0065Japan Department of Architectural Engineering Xinxiang UniversityXinxiang 453000China Gyeongsang National University 501Jinju-daeroJinjuGyeongnamRepublic of Korea

The effect of fluidity on open cell AZ31 metal foam samples fabricated using a solid state space holder method was *** was fabricated by plaster powder and perlite *** were sieved which compacted layer by layer in casting *** which made by granules were then heated to 200℃,300℃,400℃,500℃ under a rough(mechanical)vacuum before pressure infiltration with AZ31 Mg *** were infiltrated using vacuum pressure values range of 8-14 *** conditions of pressure and temperature were performed in order to get different fluidity on manufacturer of open cell AZ31 metal foam specimen *** is found that the fluidity plays significant role to determine the open cell AZ31 alloy *** fluidity is affected by preheat temperature and infiltration vacuum *** macrostructure,the distribution of the pores,microstructure,infiltration length,and porosity were investigated.

关键词： Magnesium alloy Fluidity Open cell Vacuum

Synthesis and Properties of Magnetic Nanoparticles

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Journal of Materials Science & Technology 2006年第5期22卷 659-663页

作者： Sixin LI Jiancheng ZHANG yue SHEN bo NI Jingang ZHANG College of Materials Science and Engineering Shanghai University Shanghai 200072China

The uniform mesoporous SBA-15 consisting of SiO2 with long-range channels Offers an excellent host material to synthesize or assemble the magnetic nanocomposites, such as Fe, Ni. In this paper, highly dispersed and uniform iron nanoparticles were incorporated into the pore channels of SBA-15 through a newly developed strategy in which some kinds of coupling agents were used to entrap the nanoparticles into the silica framework. The X-ray diffraction （XRD）, fourier transmission infrared spectroscopy （FTIR）, high-resolution transmission electronic microscopy （HRTEM） and energy dispersive X-ray spectroscopy （EDX） were performed to further identify the successful incorporation and grafting of iron. Compared with other ordinary non-assembled magnetic nanoparticles, the assembled Fe nanoparticles with the diameter even in the size range of 5～6 nm still have better magnetic properties.

关键词： Mesoporous materials Nanoparticle Magnetic properties

Structural Synthesis of Parallel Mechanisms with High Rotational Capability

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Chinese Journal of Mechanical Engineering 2018年第4期31卷 73-83页

作者： Xiao-Dong Jin yue-Fa Fang Sheng Guo Hai-bo Qu School of Mechanical Electronic and Control EngineeringBeijing

Most parallel mechanisms(PMs) encountered today have a common disadvantage, i.e., their low rotational *** order to develop PMs with high rotational capability, a family of novel manipulators with one or two dimensional rotations is proposed. The planar one-rotational one-translational(1 R1 T) and one-rotational two-translational(1 R2 T)PMs evolved from the crank-and-rocker mechanism(CRM) are presented by means of Lie group theory. A spatial 2 R1 T PM and a 2 R parallel moving platform with bifurcated large-angle rotations are proposed by orthogonal combination of the RRRR limbs. According to the product principle of the displacement group theory, a hybrid 2 R3 T mechanism in possession of bifurcated motion is obtained by connecting the 2 R parallel moving platform with a parallel part, which is constructed by four 3 T1 R kinematic chains. The presented manipulators possess high rotational capability. The proposed research enriches the family of spatial mechanisms and the construction method provides an instruction to design more complex mechanisms.

关键词： Parallel mechanism Rotational capability Lie group theory Bifurcated motion Hybrid mechanism

Rational SU(3) Gaudin Model

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Communications in Theoretical Physics 2001年第2期36卷 139-144页

作者： Cao Jun-Peng Hou bo-Yu yue Rui-Hong Institute of Modern Physics Northwest University Xi'an 710069 China

The Hamiltonians of the SU(3) Gaudin model are constructed based on the nonrelativistic limit of the SU(3) chain. After the quantum determinant being well defined, the eigenvectors and eigenvalues of the Hamiltonians of the SU(3) Gaudin model are given. These results can be generalized to any number of constituting spins (SU( N) ).

关键词：量子力学 SU(3)群自旋链