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Tissue Distribution and Purification of Prophenoloxidase in Larvae of Asian Corn Borer, Ostrinia furnacalis Guenée (Lepidoptera: Pyralidae)

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 360-364页

作者： cong-jing feng1,2 and wen-jun fu1* 1institute of plant physiology and ecology. shanghai institute for biological sciences. the chinese academy of sciences. shanghai 200025, china 2The GraduateSchool of the chinese academy of sciences. Beijing 100039, china institute of plant physiology and ecology Shanghai Institute for Biological Sciences the Chinese Academy of Sciences The Graduate School of the chinese academy of sciences

Using ammonium sulphate precipitation, Blue-Sepharose CL-6B, Phenyl-Sepharose CL-4B, prophenoloxidase (PPO) was isolated and purified from hemolymph of Ostrinia furnacalis larvae. This zy- mogen was a heterodimer, and composed of two subunits with the relative molecular mass ranging from 66.2 kD to 97.4 kD determined by SDS-PAGE. Western blotting and indirect immunofluorescence test showed that PPO was present in integument, hemolymph plasma and cell membrane of granular hemocytes and oenocytoids of O. furnacalis larvae.

关键词： Ostrinia furnacalis prophenoloxidase tissue distribution purification

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Role of venom and ovarian proteins in immune suppression of Ostrinia furnacalis （Lepidoptera： Pyralidae） larvae parasitized by Macrocentrus cingulum （Hymenoptera： Braconidae）, a polyembryonic parasitoid

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Insect Science 2007年第2期14卷 93-100页

作者： YONG LI JIAN-feng LU cong-jing feng XIN KE wen-jun fu institute of plant physiology and ecology Chinese Academy of Sciences Shanghai School of Bioteclmology and Food Engineering Hefei University of Technology Hefei School of Horticulture and plant Protection Yangzhou University Yangzhou China

Venom and ovarian proteins in braconid and ichneumonid wasps play an important role in the successful parasitism of their host, especially for immune suppression immediately after oviposition. In this study, we compared the effect of venom and ovarian proteins collected from the female wasps of Macrocentrus cingulum, a polyembryonic parasitoid of the larvae of Ostriniafunacalis, on the encapsulation capacity of Sephadex A- 25 beads at 4 h and 24 h post-injection both in vivo and in vitro. The results showed that the ovarian proteins significantly interfered with the encapsulation capacity of hemocytes in a dose-dependent manner. Spreading and viability of hemocytes in O. furnacalis was not disrupted by venom and ovarian proteins at various concentrations injected. It seems likely that the ovarian proteins from M. cingulum play a role in suppressing the encapsulation capacity of host hemocytes.

关键词： encapsulation Macrocentrus cingulum Ostriniafunacalis ovarian proteins spreading venom

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Cotton genome: challenge into the polyploidy

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Science Bulletin 2017年第24期62卷 1622-1623页

作者： Zhi-wen Chen jun-feng Cao Xiu-Fang Zhang Xiao-Xia Shangguan Ying-Bo Mao Ling-Jian Wang Xiao-Ya Chen National Key Laboratory of plant Molecular Genetics and National Center for plant Gene Research Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences plant Science Research Center Shanghai Chenshan Botanical Garden Shanghai Key Laboratory of Plant Functional Genomics and Resources

Cottons are the most important fiber crops in the *** cotton genus Gossypium has 52 species,including seven allotetraploid species and 45 *** species were domesticated and remain as crops under cultivation today:the New World allopolyploid species *** and ***(2n=52),and the Old World diploid species *** and ***(2n=26).The primary cultivated species is Upland cotton(*** L.),which accounts for more than 90%of global cotton fiber production.

关键词： Cotton genome challenge into the polyploidy

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Morphology and structure of the ovipositor in Macrocentrus cingulum （Hymenoptera： Braconidae）, a polyembryonic parasitoid

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Journal of Life sciences 2007年第1期1卷 34-39页

作者： LI Yong LU Jian-feng KE Xin fu wen-jun institute of plant physiology and ecology Chinese Academy of Sciences Shanghai 200032 China School of Biotechnology and Food Engineering Hefei University of Technology Hefei 230009 China

The ovipositor of parasitic insect is a highly specialized organ and crucial for parasitising. Morphology and structure of the ovipositor in female Macrocentrus cingulum, a polyembryonic parasitoid, are observed in SEM studies. The ovipositor of M. cingulum consists of the valvulae 1, 2 and 3. The valvulae 1 and 2 of ovipositor house in the valvulae 3 at rest and the valvulae 1 and 2 part from the valvulae 3 at probing and ovipositing. The cross-section of valvulae 3 shows that the valvulae 3 is hollow which may reduce the weight of ovipositor and make the ovipositor more flexible. The distal end of stylus （valvulae l, 2） becomes extremely sharp and the piercing tip of valvulae 1 and 2 form the arrangement of tongue-and-groove. This arrangement may support valvulae 2 slide longitudinally along the valvulae 1. Numbers of pores are distributed on the cuticle of the valvulae l and 2. The piecing tip of valvulae 1 and 2 have more pores than proximal and intermediate region. The out side of valvulae 3 is completely covered with chactica sensilla and the distal end of valvulae 3 has more sensilla than proximal and intermediate region. The function and structure of ovipositor of M. cingulum was discussed and compared with other species.

关键词： Macrocentrus cingulum parasitoid ovipositor

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