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Heterosis and combining ability evaluation for growth traits of blunt snout bream(Megalobrama amblycephala)when crossbreeding three strains

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Chinese Science Bulletin 2014年第9期59卷 857-864页

作者： Wei Luo cong zeng Shaokui Yi Nicholas Robinson Weimin Wang Zexia Gao College of Fisheries Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education/Key Laboratory of Freshwater Animal Breeding Ministry of Agriculture Huazhong Agricultural University Nofima PO Box 5010 1432 s Norway Collaborative Innovation Center for Healthy Freshwater Aquaculture of Hubei Province

The blunt snout bream(Megalobrama amblycephala)is a major aquaculture species in the Chinese freshwater polyculture *** this study,complete diallel crossing of three strains(i.e.,Liangzi[LZ],Poyang[PY]and Yuni[YN])was used to evaluate the combining ability and heterosis effect of intraspecific crossbreeding on the growth performance(i.e.,body weight,total length,body length,body height,and Fulton’s coefficient of condition).The offspring produced from the three strains were reared in a communal pond for 20 months,and nine microsatellites were used to assess their *** the749 offspring,708(94.53%)could be assigned directly to a single parental *** differences were observed in body weight(BW)among the different combinations,indicating that the combining ability of BW from each strain was significantly *** general combining ability(GCA)of BW from the sire was much higher than that from the *** GCA for the dam and sire of the LZ and YN were the largest and the smallest,*** special combining ability of YN$9 PY#was the largest,and YN$9 PY#showed significantly positive heterosis effects on all F1growth traits,except for Fulton’s condition coefficient(K)(P\0.05).In addition,significant positive linear correlations were found between the mean BW of corresponding progeny and the H E of the parental generation(y=600.7x-29.472,r=0.8651,P=0.0026),H O(y=1206.9x-518.14,r=0.7436,P=0.0216),and the polymorphism information content(y=1021.3x-331.31,r=0.8245,P=0.0063)as determined by a correlative *** findings obtained from the study will be important for future considerations of *** stock improvement programs.

关键词：杂种优势效应特殊配合力生长性状双列杂交团头鲂平均体重评价线性相关关系

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Two missense STK11 gene variations impaired LKB1/adenosine monophosphate-activated protein kinase signaling in Peutz-Jeghers syndrome

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1532-1546页

作者： Jin Liu Si-cong zeng An Wang Hai-Ying Cheng Qian-Jun Zhang Guang-Xiu Lu Hunan Guangxiu Hospital Hunan Normal UniversityChangsha 410000Hunan ProvinceChina Scientific Research Department Reproductive and Genetic Hospital of Citic-XiangyaChangsha 410000Hunan ProvinceChina

BACKGROUND Peutz-Jeghers syndrome(PJS)is a rare hereditary neoplastic disorder mainly associated with serine/threonine kinase 11(STK11/LKB1)gene *** genetic testing can protect a patient’s offspring from mutated genes;however,some variations in this gene have been interpreted as variants of uncertain significance(VUS),which complicate reproductive decision-making in genetic *** To identify the pathogenicity of two missense variants and provide clinical *** Whole exome gene sequencing and Sanger sequencing were performed on the peripheral blood of patients with PJS treated at the Reproductive and Genetic Hospital of *** was employed to predict the protein structure,conservation,and pathogenicity of the two missense variation sites in patients with ***,plasmids were constructed and transfected into HeLa cells to observe cell *** differences in signal pathway expression between the variant group and the wild-type group were compared using western blot and *** analysis was performed using one-way analysis of variance.PG(***297Gly)and c.733C>T(***245Phe)]in 9 unrelated PJS families who were seeking reproductive *** two missense VUS were located in the catalytic domain of serine/threonine kinase,which is a key structure of the liver kinase B1(LKB1)*** vitro experiments showed that the phosphorylation levels of adenosine monophosphate-activated protein kinase(AMPK)at Thr172 and LKB1 at Ser428 were significantly higher in transfected variation-type cells than in wild-type *** addition,the two missense STK11 variants promoted the proliferation of HeLa *** immunohistochemical analysis showed that phosphorylated-AMPK(Thr172)expression was significantly lower in gastric,colonic,and uterine polyps from PJS patients with misse

关键词： Missense STK11 Peutz-Jeghers syndrome Rare disease Genetic counseling Assisted reproductive technique

Impact of anthropogenic activities on changes of ichthyofauna in the middle and lower Xiang River

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Aquaculture and Fisheries 2022年第6期7卷 693-702页

作者： cong zeng Yao Wen Xinhua Liu Jianbo Yu Binsong Jin Deliang Li College of Animal Science and Technology Hunan Agricultural UniversityChangsha410128China School of Oceanography Shanghai Jiao Tong UniversityShanghai200030China National Ecosystem Research Station of Jiangxi Poyang Lake Wetland Institute of Life ScienceNanchang UniversityNanchang330031China

With the population increasing,fish communities,as important components in the river system,were inescapably impacted by human *** investigate these impacts,we characterized the fish community’s composition by environmental DNA metabarcoding in the urban and suburban sites along Xiang River in central *** results showed that 32 species were identified,and most species were from *** analysis indicated no significant differences in fish communities among the three urban ***,significant differences were observed between the bank solidified and non-solidified areas,which indicated that the channelization significantly influenced the biodiversity and *** alien species,Clarias gariepinus,was discovered in all examined sites,and it was negatively associated with ten native ***,human populations and acidification were also negatively related to *** findings clearly showed that the consequences of anthropogenic activities shaped ichthyofauna and caused biodiversity loss in the urban river.

关键词： Environmental DNA Human disturbances Fish communities Xiang river Biodiversity Conservation

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基于机器视觉的轻型梁三维振动测量方法

北京航空航天大学学报 2021年第2期47卷 207-212页

作者：彭聪缪卫东曾聪南京航空航天大学自动化学院南京211106

振动测量是状态检测和故障诊断的方法之一,针对传统接触式测量方法中存在负载效应等问题,对基于机器视觉的三维振动测量方法进行了研究。首先,基于视频相位的二维振动测量方法,提取出相机所采集图像中被测目标的二维振动数据。然后,在... 详细信息

振动测量是状态检测和故障诊断的方法之一,针对传统接触式测量方法中存在负载效应等问题,对基于机器视觉的三维振动测量方法进行了研究。首先,基于视频相位的二维振动测量方法,提取出相机所采集图像中被测目标的二维振动数据。然后,在二维振动测量方法的基础上,结合双目立体视觉,设计了一种基于机器视觉的三维振动测量方法。最后,进行了悬臂梁的振动测量实验。结果表明:所提方法可以实现无接触和无标记的振动测量,并能准确测量出三维的振动信息。

关键词：振动测量三维振动信号机器视觉图像处理轻质结构

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Susceptibility mapping of ground collapse caused by anthropogenic activities

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Journal of Earth Science 2022年

作者： Zhong Min Mao Yu Yong Jiao Fei Tan Xin Qi cong zeng Faculty of Engineering， China University of Geosciences Wuhan Center， China Geological Survey

Frequent ground collapses resulted by anthropogenic activities occur due to rapid urbanization. Accurate susceptibility mapping is critical for disaster prevention and control. In this study， 1198 ground collapse cases were collected from 2017 to 2020 in Shenzhen. After multicollinearity testing， eight effective factors （elevation， relief， clay proportion， average annual precipitation， distance from water， land use type， building density， and road density） were selected to construct an evaluation index system. Ground collapse susceptibility was analyzed and mapped using the normalized frequency ratio （NFR）， logistic regression （LR）， and NFR-LR coupling models. Finally， the rationality and performance of the three models were compared using the frequency ratio （FR） and receiver operating characteristic （ROC） curve， respectively. All three models could effectively evaluate the ground collapse susceptibility (area under the ROC curve （AUC） > 0.7)， though the result of NFR-LR model was more rational and had the highest performance （AUC = 0.791） among the three models. Shenzhen possessed ground collapses mainly in built-up areas， the greater the intensity of anthropogenic activity in a location， the higher the likelihood of a disaster. The high and very high susceptibility zones covered a total area of 546.76 km;and involved the Nanshan， Luohu， and Futian Districts， as well as some areas of the Baoan， Guangming， and Longgang Districts.

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Defects in phospholipase C zeta cause polyspermy and low fertilization after conventional IVF:not just ICSI failure

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Asian Journal of Andrology 2024年第2期26卷 175-182页

作者： Jian-Fang Che Hui-Xia Wu Si-cong zeng Yue-Ren Wu Jing Dai De-Hua Cheng Fei Gong Guang-Xiu Lu Ge Lin Can Dai School of Medicine Hunan Normal UniversityChangsha 410013China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410008China Clinical Research Center for Reproduction and Genetics in Hunan Province Changsha 410078China National Engineering and Research Center of Human Stem Cell Changsha 410205China

Phospholipase C zeta(PLC)is a key sperm-borne oocyte-activating factor that triggers Ca^(2+)oscillations and the subsequent block to polyspermy following gamete *** in PLCZ1,the gene encoding PLCζ,cause male infertility and intracytoplasmic sperm injection(ICSI)fertilization failure;and PLCζ expression and localization patterns are significantly correlated with ICSI fertilization rate(FR).However,in conventional in vitro fertilization(cIVF),whether and how sperm PLCζ affects fertilization remain ***,we identified one previously reported and two novel PLCZ1 mutations associated with polyspermy in vitro that are characterized by excessive sperm-zona binding and a delay in pronuclei(PN)*** staining and oocyte activation testing revealed that virtually all spermatozoa from patients lacked functional PLCζ and were thus unable to evoke Ca^(2+) *** with an artificial oocyte activation treatment successfully rescued the polyspermic phenotype and resulted in a live ***,we analyzed PLCζ in an additional 58 males after cIVF treatment in the Reproductive and Genetic Hospital of CiTiC-Xiangya(Changsha,China)between February 2019 and January *** found that the proportion of spermatozoa that expressed PLCζ was positively correlated with both 2PN rate and total *** optimal cutoff value below which males were likely to experience low FR(total FR≤30%)after clVF was 56.7%for the proportion of spermatozoa expressing *** study expands the mutation and the phenotypic spectrum of PLCZ1 and further suggests that PLCζ constitutes a promising biomarker for identifying low FRs cases in cIVF due to sperm-related oocyte activation deficiency and that sperm PLCζ analysis may benefit the widermale population and not onlymen with IcsI failure.

关键词： conventional IVF gene variants PLCζ polyspermy

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论个人信息与数据的位阶保护模式

中国特色社会主义研究 2022年第5期 131-142页

作者：曾聪中国政法大学刑事司法学院

个人信息与数据存在区别。个人信息属于私法权利,强调可识别某个自然人的内容要素,适配以同意原则为中心构建的私法控制模式;数据属于公法权益,强调对内容要素的规模化抓取与数字化处理过程,适配以合理利用原则为中心构建的公法保护模... 详细信息

个人信息与数据存在区别。个人信息属于私法权利,强调可识别某个自然人的内容要素,适配以同意原则为中心构建的私法控制模式;数据属于公法权益,强调对内容要素的规模化抓取与数字化处理过程,适配以合理利用原则为中心构建的公法保护模式。应当根据个人信息转化为数据的发展过程,采取位阶式的综合保护模式:个人信息规模化、数字化之前,以私法控制模式为主;个人信息规模化、数字化过程中,整合私法控制的端口式保护与公法规制的过程式保护;个人信息规模化、数字化之后,以公法利用模式为主。

关键词：个人信息数据控制模式利用模式位阶式保护

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生成式人工智能与“不可解释性”危害结果的客观归属

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华中科技大学学报（社会科学版） 2024年第5期38卷 53-62,112页

作者：曾聪中国政法大学刑事司法学院

生成式人工智能的有限自主性源于以大数据为基础的算法决策的不可解释性,意味着其可以独立造成法益侵害。既有理论深陷人工智能体有无责任能力的主观归责问题,却忽视了生成式人工智能犯罪首先面临的是危害结果之客观归属问题。生成式人... 详细信息

生成式人工智能的有限自主性源于以大数据为基础的算法决策的不可解释性,意味着其可以独立造成法益侵害。既有理论深陷人工智能体有无责任能力的主观归责问题,却忽视了生成式人工智能犯罪首先面临的是危害结果之客观归属问题。生成式人工智能不是强人工智能,其所造成的法益侵害无法归属于智能体本身,应当根据《生成式人工智能服务暂行管理办法》等技术规范,在生产者与使用者之间分配生成式人工智能所致法益侵害的风险,以填补刑法处罚漏洞。风险分配的依据是技术规范在科技发展与人类安全之间的价值判断立场,其规定了生产者与使用者对不可解释性算法决策可能存在“最差的情形”的注意义务。风险分配的方式是在准备、运算与生成这三个阶段,结合当前人工智能犯罪的不同类型,在生产者与使用者之间进行不对称的风险分配。为避免过度限制科技发展,应根据下位规则限制危害结果的客观归属,一是根据溯责禁止原则限制对生产者的结果归属,二是根据有限的自我答责原则限制对使用者的结果归属。

关键词：生成式人工智能不可解释性客观归属风险分配

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Analysis and validation of diagnostic biomarkers and immune cell infiltration characteristics in pediatric sepsis by integrating bioinformatics and machine learning

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World Journal of Pediatrics 2023年第11期19卷 1094-1103页

作者： Wen‑Yuan Zhang Zhong‑Hua Chen Xiao‑Xia An Hui Li Hua‑Lin Zhang Shui‑Jing Wu Yu‑Qian Guo Kai Zhang cong‑Li zeng Xiang‑Ming Fang Department of Anesthesiology and Intensive Care School of MedicineThe First Affiliated HospitalZhejiang UniversityQingChun Road 79Hangzhou 310003China Department of Anesthesiology Shaoxing People’s Hospital(Shaoxing HospitalZhejiang University School of Medicine)Shaoxing 312000China Department of Anesthesiology Vagelos College of Physicians and SurgeonsColumbia UniversityNew YorkNYUSA

Background Pediatric sepsis is a complicated condition characterized by life-threatening organ failure resulting from a dysregulated host response to infection in *** is associated with high rates of morbidity and mortality,and rapid detection and administration of antimicrobials have been *** objective of this study was to evaluate the diagnostic biomarkers of pediatric sepsis and the function of immune cell infiltration in the development of this *** Three gene expression datasets were available from the Gene Expression Omnibus ***,the differentially expressed genes(DEGs)were found with the use of the R program,and then gene set enrichment analysis was carried ***,the DEGs were combined with the major module genes chosen using the weighted gene co-expression *** hub genes were identified by the use of three machine-learning algorithms:random forest,support vector machine-recursive feature elimination,and least absolute shrinkage and selection *** receiver operating characteristic curve and nomogram model were used to verify the discrimination and efficacy of the hub *** addition,the inflammatory and immune status of pediatric sepsis was assessed using cell-type identification by estimating relative subsets of RNA transcripts(CIBERSORT).The relationship between the diagnostic markers and infiltrating immune cells was further *** Overall,after overlapping key module genes and DEGs,we detected 402 overlapping *** pediatric sepsis diagnostic indicators,CYSTM1(AUC=0.988),MMP8(AUC=0.973),and CD177(AUC=0.986)were investigated and demonstrated statistically significant differences(P<0.05)and diagnostic efficacy in the validation *** indicated by the immune cell infiltration analysis,multiple immune cells may be involved in the development of pediatric ***,all diagnostic characteristics may correlate with immune cells to varying *** The candida

关键词： Paediatric sepsis Bioinformatics Machine-Learning Biomarkers Immune cell infiltration