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Modeling and analysis of nano-sized gMRs based on Co,NiFe and Ni materials

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Science China(Information Sciences) 2014年第2期57卷 216-229页

作者： YIN cong JIA ze MA WeiChao REN TianLing Institute of Microelectronics Tsinghua National Laboratory for Information Science and TechnologyTsinghua University

Magnetic simulation method is introduced to analyze.giant magnetoresistances(gMRs)in nanoscale for nano-sized biosensors.A spin valve model with special gridding corresponding to the exchange interaction length is proposed to study the influence of easy axes,exchange coefcients,pinning fields and feature widths on magnetization reversals and hysteresis characteristics of nano-sized gMRs with diferent pinned layer and free layer materials of Co,NiFe and *** switching field is found to be almost linear with the pinning field and decrease with the absolute exchange coefcients and the feature widths for the nano-sized *** increase rate of each depends on the spin valve *** investigations into variations of the magnetization distribution reveal that the initial magnetization distribution and the magnetization reversal mode depend greatly on easy axes and materials The dependence on easy axes based mainly on the magnetocrystalline anisotropy is illustrated in detail.

关键词： nano-sized spin valve magnetization reversal switching field hysteresis antiferromagnetic coupling

RT-qPCR normalization of reference genes in different lifehistory stages of gracilaria vermiculophylla(Rhodophyta)

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Journal of Oceanology and Limnology 2023年第5期41卷 1910-1917页

作者： Yingyue ZHANg Jinxin YANg ze YANg cong QI Di XU Key Laboratory of Marine genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China

The quantitative realtime polymerase chain reaction(RT-qPCR)is a powerful and sensitive method to measure expression of targeted gene but it highly relies on the use of suitable reference genes for data *** evaluated the expressions of 8 housekeeping genes:18S ribosomal rDNA(18S rDNA),28S ribosomal r DNA(28S rDNA),rubisco large subunit(rbc-L),β-actin(ACT),glyceraldehyde-3-phosphate dehydrogenase(gAPDH),elongation factor 1(EF1),β-tubulin(Tub B),and P-phycoerythrin B(PEB),to select the suitable reference genes for different life-history stages(tetrasporophyte,carposporophyte,and male/female gametophyte)of gracilaria vermiculophylla by absolute quantitative *** geNorm and BestKeeper were used to verify the results acquired from copy number *** show that the expression of identified reference genes varied in comparing groups composed of different type of life *** is suggested that 18S rDNA and TubB could be used for highly complex samples composed of mixed ploidy and phases.18S rDNA and 28S rDNA were also preferred for using among the matured isomorphic *** for samples with different maturities,TubB and ACT were recommended for tetrasporophytes and gametophytes respectively.

关键词： reference gene gracilaria vermiculophylla life-history stage quantitative real-time polymerase chain reaction(RT-qPCR) red algae

Magnetoresistive behavior and magnetization reversal of NiFe/Cu/CoFe/IrMn spin valve gMRs in nanoscale

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International Journal of Minerals,Metallurgy and Materials 2013年第7期20卷 700-704页

作者： cong Yin ze Jia Wei-chao Ma Tian-ling Ren Institute of Microelectronics & Tsinghua National Laboratory for Information Science and Technology Tsinghua University

The magnetoresistance behavior and the magnetization reversal mode of NiFe/Cu/CoFe/IrMn spin valve giant magnetoresistance （SV-gMR） in nanoscale were investigated experimentally and theoretically by nanosized magnetic simulation methods. Based on the Landau-Lifshitz-gilbert equation, a model with a special gridding was proposed to calculate the giant magnetoresistance ratio （MR） and investigate the magnetization reversal mode. The relationship between MR and the external magnetic field was obtained and analyzed. Studies into the variation of the magnetization distribution reveal that the magnetization reversal mode, that is, the jump variation mode for NiFe/Cu/CoFe/IrMn, depends greatly on the antiferromagnetic coupling behavior between the pinned layer and the antiferromagnetic layer. It is also found that the switching field is almost linear with the exchange coefficient.

关键词： giant magnetoresistance (gMR) spin valves nanoscale magnetization reversal

Effects of acid-rock reaction on physical properties during CO_(2)-rich industrial waste gas(CO_(2)-rich IWg)injection in shale reservoirs

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Petroleum Science 2024年第1期21卷 272-285页

作者： Yi-Fan Wang Jing Wang Hui-Qing Liu Xiao-cong Lv ze-Min Ji National Key Laboratory of Petroleum Resources and Engineering China University of PetroleumBeijing102249China MOE Key Laboratory of Petroleum Engineering China University of PetroleumBeijing102249China Research Institute of Exploration and Development PetroChinaBeijing100083China

"Carbon peaking and carbon neutrality"is an essential national strategy,and the geological storage and utilization of CO_(2)is a hot issue ***,due to the scarcity of pure CO_(2)gas sources in China and the high cost of CO_(2)capture,CO_(2)-rich industrial waste gas(CO_(2)-rich IWg)is gradually emerging into the public's ***_(2)has good adsorption properties on shale surfaces,but acidic gases can react with shale,so the mechanism of the CO_(2)-rich IWg-water-shale reaction and the change in reservoir properties will determine the stability of geological ***,based on the mineral composition of the Longmaxi Formation shale,this study constructs a thermodynamic equilibrium model of water-rock reactions and simulates the regularity of reactions between CO_(2)-rich IWg and shale *** results indicate that CO_(2)consumed 12%after reaction,and impurity gases in the CO_(2)-rich IWg can be dissolved entirely,thus demonstrating the feasibility of treating IWg through water-rock *** IWg inhibits the dissolution of CO_(2),the optimal composition of CO_(2)-rich IWg is 95%CO_(2)and 5%IWg when CO_(2)geological storage is the main *** contrast,when the main goal is the geological storage of total CO_(2)-rich IWg or impurity gas,the optimal CO_(2)-rich IWg composition is 50%CO_(2)and 50%*** the CO_(2)-rich IWg-water-shale reaction,temperature has less influence on the water-rock reaction,while pressure is the most important ***2 has the greatest impact on water-rock reaction in *** minerals,clay minerals such as illite and montmorillonite had a significant effect on water-rock *** overall reaction is dominated by precipitation and the volume of the rock skeleton has increased by 0.74 cm3,resulting in a decrease in shale porosity,which enhances the stability of CO_(2)geological storage to some *** the reaction between CO_(2)-rich IWg-water-shale at simulated temperatures and pressures,precipitation

关键词： CO_(2)-rich industrial waste gas geological storage Acid-rock reaction Shale geochemical modelling

Nitrogen acquisition,fixation and transfer in maize/alfalfa intercrops are increased through root contact and morphological responses to interspecies competition

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Journal of Integrative Agriculture 2021年第8期20卷 2240-2254页

作者： SHAO ze-qiang ZHENg cong-cong Johannes APOSTMA LU Wen-long gAO Qiang gAO Ying-zhi ZHANg Jin-jing College of Resources and Environmental Sciences Jilin Agricultural University/Key Laboratory of Sustainable Utilization of Soil Resources in the Commodity Grain Bases in Jilin ProvinceChangchun 130118P.R.China College of Resource and Environment Engineering Jilin Institute of Chemical TechnologyJilin 132022P.R.China Forschungszentrum Jülich gmbH Institute of Bio-and Geosciences-Plant Sciences(IBG-2)Jülich 52428Germany Key Laboratory of Vegetation Ecology Northeast Normal UniversityChangchun 130024P.R.China

Nitrogen(N)fixation by legumes and nitrogen transfer to cereals have been considered as important pathways for overyielding and higher N use efficiency in cereal/legume intercropping ***,the extent to which root morphology contributes to N fixation and transfer is unclear.A two-factorial greenhouse experiment was conducted to quantify the N fixation,transfer and root morphology characteristics of the maize/alfalfa intercropping system in two consecutive years using the 15N-urea leaf labeling method,and combining two N levels with three root separation techniques.N application could inhibit N fixation and transfer in a maize/alfalfa intercropping *** of the N application level,compared with plastic sheet separation(PSS),no separation(NS)and nylon mesh separation(NNS)significantly increased the total biomass(36%)and total N content(28%),while the N fixation rate also sharply increased by 75 to 134%,and the amount of N transferred with no root barrier was 1.24–1.42 times greater than that with a mesh *** analysis(RDA)showed that the crown root dry weight(CRDW)of maize and lateral root number(LRN)of alfalfa showed the strongest associations with N fixation and *** results highlight the importance of root contact for the enhancement of N fixation and transfer via changes in root morphology in maize/alfalfa intercropping systems,and the overyielding system was achieved via increases in maize.growth,at the cost of smaller decreases in alfalfa biomass production.

关键词： maize/alfalfa intercropping nitrogen fixation and transfer root morphology nitrogen utilization

Effects of hypobaric hypoxia on adenine nucleotide pools,adenine nucleotide transporter activity and protein expression in rat liver

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World Journal of gastroenterology 2006年第13期12卷 2120-2124页

作者： cong-Yang Li Jun-ze Liu Li-Ping Wu Bing Li Li-Fen Chen Department of Pathophysiology Institute of High Altitude Military Medicine Third Military Medical University Chongqing 400038 China Xijing Hospital the Fourth Military Medical University Xi'an 710032 Shaanxi Province China

AIM： To explore the effect of hypobaric hypoxia on mitochondrial energy metabolism in rat liver. METHODS： Adult male Wistar rats were exposed to a hypobadc chamber simulating 5000 m high altitude for 23 h every day for 0 （H0）, 1 （H1）, 5 （H5）, 15 （H15） and 30 d （H30） respectively. Rats were sacrificed by decapitation and liver was removed. Liver mitochondria were isolated by differential centrifugation program. The size of adenine nucleotide pool （ATP ADP, and AMP） in tissue and mitochondria was separated and measured by high performance liquid chromatography （HPLC）. The adenine nucleotide transporter （ANT） activity was determined by isotopic technique. The ANT total protein level was determined by Western blot. RESULTS： Compared with H0 group, intra-mitochondrial ATP content decreased in all hypoxia groups. However, the H5 group reached the lowest point （70.6%）（P〈 0.01） when compared to the control group. Intra-mitochondrial ADP and AMP level showed similar change in all hypoxia groups and were significantly lower than that in H0 group, In addition, extra-mitochondrial ATP and ADP content decreased significantly in all hypoxia groups. Furthermore, extra-mitochondrial AMP in groups H5, H15 and H30 was significantly lower than that in H0 group, whereas HI group had no marked change compared to the control situation. The activity of ANT in hypoxia groups decreased significantly, which was the lowest in H5 group （55.7%）（P〈0.01） when compared to H0 group. ANT activity in H30 group was higher than in H15 group, but still lower than that in H0 group. ANT protein level in H5, H15, H30 groups, compared with H0 group decreased significantly, which in H5 group was the lowest, being 27.1% of that in H0 group （P〈0.01）. ANT protein level in H30 group was higher than in H15 group, but still lower than in H0 group. CONCLUSION： Hypobaric hypoxia decreases the mitochondrial ATP content in rat liver, while mitochondrial ATP level recovers during long-term hypoxia exposure. The lower

关键词： Adenine nucleotide pool Hypoxia Liver Mitochondria

Effects of different ingredients of zedoary on gene expression of HSC-T6 cells

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World Journal of gastroenterology 2005年第43期11卷 6780-6786页

作者： Yuan Jiang ze-Song Li Fu-Sheng Jiang Xin Deng cong-Shun Yao guang Nie Shenzhen Shajing People's Hospital Shenzhen 518104 Guangdong Province China Shenzhen Yishengtang Biology Enterprise Company Shenzhen 518126 Guangdong Province China Shenzhen Donghu Hospital Shenzhen 518020 Guangdong Province China Teaching pharmacology and Research Section of Shenyang Pharmaceutial University Shenyang 110000 Liaoning Province China

AIM： To investigate the effects of four different ingredients of zedoary （Curcuma aromatica oil, Curcumol, β-elemence, and Curcumin） on the gene expressions of hepatic stellate cells （HSCs）, and to explore the molecular mechanism of zedoary against hepatic fibrosis at gene network level. METHODS： We detected the mRNA sequences of 50 liver fibrosis-related genes in genBank and designed oligonucleotide probes. We synthesized oligonucleotides with PE8909 DNA synthesizing instrument, and carried out oligonucleotide microarray with OgR-04 dropping instrument and aldehyded glass chip. Cultured HSC-T6 cells were breated wibh different concentrations of Colchicine, Curcuma aromatica oil, Curcumol, β-elemence, and Curcumin. According to the experiment of cell toxicity, we took the appropriate concentrations of medicines that resulted in over 50% of cell survival as experiment concentrations. We collected the cells at 1, 6, 12, and 24 h, and extracted total RNA with TRIzol reagent, then labeled cDNAs with Cy3-dUTP and Cy5-dUTP. These labeled cDNAs were hybridized to an oligonucleotide microarray which was washed several times and scanned by scanner genePix 4000B. Different gene expressions of HSC-T6 cells were analyzed by Imagene 4.2 software. RESULTS： After HSC-T6 cells were cultured in a medium containing 6.25μg/mL Colchicine for 12 h, expression of TIMP-1 decreased 2.2-folds. After HSC-T6 cells were cultured in a medium containing 78.125 μg/mL of Curcuma aromatica oil for 24 h, the expression of TIMP-2 and IL-6 decreased 2.3- and 2.2-folds, respectively. Moreover, after HSC-T6 cells were cultured in a medium containing 1.5625 μg/mL of Curcumol for 12 h, the expression of TgFμ1 and P450a decreased 2.3- and 2.1-folds, respectively. CONCLUSION： Our results may show the possible molecular mechanism of Curcuma aromatica oil and Curcumol against hepatic fibrosis.

关键词： DNA microarray Curcuma aromatica oil Curcumol Hepatic stellate cells Hepatic fibrosis

Theoretical studies of carbenes and carbenoids *** and isomerizations of carbenoid H2C=CLiF

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Chinese Journal of Chemistry 1988年第4期6卷 294-301页

作者： Wang Bing‐ze Deng cong‐Hao Xu Lin‐Xiao Department of Chemistry Shandong University Jinan Department of Chemistry Fudan University Shanghai

The vinylidene carbenoid HC=CLiF is studied with RHF/STO-3g gradient *** equilibrium states and their isomerization transition states are *** lowest energystructure 1 should be detectable in the *** Li and F lie at the same side of C=C bondis a remarkable feature of *** dipole moments,MuUiken populations and frontier molecular or-bitals are also given and analyzed.

关键词： Theoretical studies of carbenes and carbenoids 2.Structures and isomerizations of carbenoid H2C HOMO

Suppression of flux avalanches in YBCO superconducting thin films by coating metal investigated using magneto-optical imaging

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Superconductivity 2024年第3期11卷 4-13页

作者： Qianting Ran ze Jing Lei Shen Jun Zhou cong Liu Zhiwei Zhang Xingyi Zhang Key Laboratory of Mechanics on Disaster and Environment in Western China attached to the Ministry of Education of China Lanzhou UniversityLanzhouGansu 730000PR China Department of Mechanics and Engineering Sciences College of Civil Engineering and MechanicsLanzhou UniversityLanzhouGansu 730000PR China Institute of Extreme Mechanics and School of Aeronautics Northwestern Polytechnical UniversityXi’an 710072PR China

Flux avalanches,prevalently existing in superconducting thin films,can cause catastrophic breakdowns of electromagnetic properties and even irreversible damage to superconducting *** coating is an effective way to suppress the flux avalanches in superconducting thin ***,it is difficult to reveal the suppression mechanisms due to the challenge of effectively separating the simultaneous eddy currents and heat exchange in the metal *** this work,the eddy currents and heat exchange in the Ag metal coating are separated by setting a thermal insulation layer,and its inhibiting effect on the flux avalanches of the YBCO superconducting thin films is *** results indicate that eddy currents play an important part in suppressing magnetic flux avalanches,and their effect strengthens with increasing Ag ***,employing the double-exposure method,the flux avalanche velocity of YBCO superconducting thin films was measured,revealing a significant decrease in the magnetic flux avalanche velocity due to suppression by eddy ***,a theoretical model was established to investigate the influence of eddy currents on the motion of a single vortex,and the calculated results showed good agreement with the *** findings provide a better understanding of the flux avalanches and their suppression in YBCO superconducting thin films.

关键词： YBCO superconducting thin films Flux avalanches Metal coating Eddy currents Double-exposure method