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Science china chemistry 2010年第6期53卷 1453-1458页

作者： FU YingZi1,CHEN Min1,CUI Xin2,WANG LiLan1,CHEN Qiao1 & ZHOU Juan1 1key laboratory on Luminescence and Real-Time Analysis,ministry of education college of chemistry and chemical engineering,southwest university,chongqing 400715,china 2key laboratory of green chemistry & technology,ministry of education college of chemistry,Sichuan university,Chengdu 610064,china key laboratory on Luminescence and Real-Time Analysis Ministry of Education college of chemistry and chemical engineering Southwest University Chongqing China key laboratory of green chemistry & technology Ministry of Education college of chemistry Sichuan University Chengdu China

The novel nanocomposites were obtained by covalently linking arginine enantiomers with the sidewall of multi-walled carbon nanotubes,and utilized as sensing materials for biomolecular recognition in electrochemical *** were characterized by X-ray photoelectron spectroscopy (XPS),transmission electron microscopy (TEM) and circular dichroism spectroscopy (CD).Nano-gold and Prussian Blue were employed to amplify the analytical signal *** specific antibody/antigen and carcinoembryonic antibody/antigen were used as model *** results showed that the different configurations of nanocomposites could readily facilitate chiral recognition of antibodies,and D-nanocomposites displayed a larger signal.

关键词： arginine multi-walled carbon nanotubes chiral nanocomposites recognition electrochemical immunoassay

Application of L-thiazolidine-4-carboxylic acid monolayer in electrochemical determination of copper(Ⅱ)

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Science china chemistry 2010年第1期53卷 257-262页

作者： CUI Xin1,FU YingZi1,2,LI MiaoSi1,CHEN Min2,HE Xuan1,LIU XiaoHua1 & FENG XiaoMing1 1key laboratory of green chemistry & technology,ministry of education college of chemistry,Sichuan university,Chengdu 610064,china 2key laboratory of Luminescence and Real-Time Analysis,ministry of education college of chemistry and chemical engineering,southwest university,chongqing 400715,china key laboratory of green chemistry & technology Ministry of Education college of chemistry Sichuan University Chengdu China key laboratory of Luminescence and Real-Time Analysis Ministry of Education college of chemistry and chemical engineering Southwest University Chongqing China

L-Thiazolidine-4-carboxylic acid monolayer was prepared on gold electrodes through the self-assembly *** novel thioether-based monolayer could efficiently preconcentrate Cu2+,which provided a simple,stable and reproducible method for the determination of Cu2+.The modified electrodes were stable enough to be continuously used for one week(more than 30 times regeneration) with lower than 10% decrease in the *** retained their initial activity for more than one month if used once a *** calibration curve was linear for Cu2+ from 0.6 to 158.8 μg L?1 with a detection limit of 0.38 μg L?*** relative standard deviation was 3.2% for a series of six successive *** proposed method was applied in the determination of Cu2+ in mineral water and human hair samples.

关键词： L-thiazolidine-4-carboxylic acid copper ions gold electrode thioether monolayer

The interaction between furosemide-palladium (Ⅱ) chelate and basic triphenylmethane dyes by resonance Rayleigh scattering spectra and resonance non-linear scattering spectra and their analytical applications

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Science china chemistry 2010年第8期53卷 1767-1777页

作者： LI CuiXia,LIU ShaoPu,LIU ZhongFang & HU XiaoLi key laboratory on Luminescence and Real-Time Analysis,ministry of education School of chemistry and chemical engineering,southwest university,chongqing 400715,china key laboratory on Luminescence and Real-Time Analysis Ministry of Education School of chemistry and chemical engineering Southwest University Chongqing China

In pH 4.5-7.0 Britton-Robinson buffer solution,furosemide (FUR) reacted with Pd (II) to form a 1:1 anionic *** chelate could further react with such basic triphenylmethane dyes (BTPMD) as ethyl violet (EV),crystal violet (CV),methyl violet (MV),methyl green (MeG) and brilliant green (BG) to form 1:1 ion-association *** not only resulted in the change of absorption spectra,but also led to the significant enhancement of resonance Rayleigh scattering (RRS),second-order scattering (SOS) and frequency doubling scattering (FDS).The maximum RRS wavelengths were located at 324 nm for the EV,CV and MV system,and 340 nm for the BG and MeG *** maximum SOS wavelengths were located at 550 nm for the EV,CV,BG and MeG system,and 530 nm for the MV *** maximum scattering peaks of all the systems were at 392 nm for *** enhanced RRS,SOS and FDS intensities were directly proportional to the concentration of *** detection limits for the different dye systems were 0.3-4.9 ng mL-1 for the RRS method,3.2-33.1 ng mL-1 for the SOS method and 9.0-85.7 ng mL-1 for the FDS *** methods could be used for the determination of trace amounts of *** effects of the formation of ternary ion-association complexes on the spectral characteristics and intensities of absorption,RRS,SOS and FDS have been *** optimum conditions of these reactions,the influencing factors and the analytical properties have been *** influences of coexisting substances were tested by RRS method and the results showed that this method exhibited a high *** on the aforementioned research,the highly sensitive,simple and rapid methods for the determination of trace amounts of FUR by resonance light scattering technique have been established,which could be applied to the determination of FUR in tablet,injection,human serum and urine *** composition and structure of the ternary ion-association complex and the reaction mechanism were discusse

关键词： furosemide basic triphenylmethane dyes palladium (Ⅱ) resonance Rayleigh scattering resonance non-linear scattering

Aptamer-based competitive electrochemical assay of small biomolecules

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Science china chemistry 2011年第5期54卷 822-826页

作者： ZHANG YuYong,XIANG Yun,CHAI YaQin & YUAN Ruo key laboratory on Luminescence and Real-Time Analysis,ministry of education School of chemistry and chemical engineering,southwest university,chongqing 400715,china key laboratory on Luminescence and Real-Time Analysis Ministry of Education School of chemistry and chemical engineering Southwest University Chongqing China

A convenient aptamer-based competitive electrochemical biosensor for a small biomolecule,adenosine,was described. The sensing surface was fabricated by self-assembly of an aptamer/mercaptohexanol monolayer on a gold disk electrode. The principle of this aptasensor is based on the competition between an adenosine target molecule and a ferrocene-conjugated signaling DNA strand for the aptamer binding site on the sensing surface. Due to the competitive nature of this assay,the electrochemical responses of the surface captured ferrocene are inversely proportional to log[adenosine] in the range from 0.05 to 3.2 μM,with a detection limit of 25 nM. Moreover,the aptasensor also shows high selectivity for adenosine. The proposed aptasensor thus holds great potential for the detection of other small biomolecules.

关键词： aptamer adenosine biosensor competitive assay differential pulse voltammetry

The synthesis of mono-6-thio-β-cyclodextrin capped CdTe QDs and its interaction with neutral red

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Science china chemistry 2010年第6期53卷 1358-1365页

作者： WANG Lei,LIU ShaoPu,PENG JuanJuan & HE YouQiu key laboratory on Luminescence and Realtime Analysis,ministry of education School of chemistry and chemical engineering,southwest university,chongqing 400716,china key laboratory on Luminescence and Realtime Analysis Ministry of Education School of chemistry and chemical engineering Southwest University Chongqing China

This paper presents a novel method for the synthesis of CdTe quantum dots (QDs) capped with β-cyclodextrin in aqueous solution using both TGA and mono-6-thio-β-CD as *** interaction between mono-6-thio-β-CD-CdTe QDs and neutral red (NR) was studied with fluorescence,UV-absorption and the resonance Rayleigh scattering *** its concentration was over 7.5 × 10-6 mol/L,the neutral red began to aggregate on the surface of the mono-6-thio-β-CD-CdTe QDs,which resulted in the mono-6-thio-β-CD-CdTe QDs particle size increasing,the sharply quenched fluorescence,and the marked increase of RRS intensity.

关键词： mono-6-thio-β-Cyclodextrin CdTe quantum dots thioglycolic acid neutral red

Chromo-chemodosimetric detection for Fe^(2+) by Fenton reagent-induced chromophore-decolorizing of halogenated phenolsulfonphthalein derivatives

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Science china chemistry 2010年第6期53卷 1398-1405页

作者： ZHENG Kuan,LAI ChengYue,HE LiPing & LI Fang key laboratory of green chemistry and technology,ministry of education college of chemistry,Sichuan university,Chengdu 610064,china key laboratory of green chemistry and technology Ministry of Education College of Chemistry Sichuan University Chengdu China

A commercially-available sulfonphthalein derivative was demonstrated to be a chemodosimeter for Fe2+ and its sensing behavior was further investigated by UV-vis spectroscopy in aqueous media under the optimum *** the presence of chlorophenol red (CPR) and H2O2,the absorption maximum at 435 nm decreased upon addition of Fe2+,resulting in a significant color change of the CPR solution from yellow to *** chemosensor system did not show significant responses to a series of other metal ions including Al3+,Zn2+,Cd2+,Hg2+,Mn2+,Co2+,Fe3+,Ni2+,Cu2+,La3+,Ce4+,Th4+,Pd2+,Pb2+,Sb3+,Cr3+,Au3+,Ag+,Nd3+,Sm3+,alkali and alkaline earth metal cations,allowing for highly selective naked-eye detection of Fe2+.Quantitative analysis was carried out kinetically for practicable the Fe2+ assay when either fixed time method or the initial rate method was *** the detecting time was set,the decrease of absorbance signal was linear with Fe2+ concentration in the range of 0 to 7.50 × 10-5 mol L-1 and the regression equation was ΔA = 0.00759 + 0.00593CFe with a correlation coefficient r = *** chemodosimetric system has employed an irreversible Fenton reagent-promoted oxidation of the CPR free chromophore and the hydroxyl radicals were generated in the presence of both Fe2+ and hydrogen *** mechanistic interpretation of the signaling process was partially confirmed by the radical scavenging experiment and the FT-IR analysis of the intermediates formed at different reaction periods.

关键词： colorimetric chemosensor ferrous ion sulfonphthalein Fenton reagent

Homochiral expression of proteins:a discussion on the natural chirality related to the origin of life

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Science china chemistry 2010年第4期53卷 792-796页

作者： HU PingPing1,2, PENG Li2, ZHEN ShuJun3, CHEN LiQiang2, XIAO SaiJin1 & HUANG ChengZhi1,3 1key laboratory on Luminescence Real-Time Analysis, ministry of education college of chemistry and chemical engineering, southwest university, chongqing 400715, china 2college of Life Science, southwest university, chongqing 400715, china 3college of Pharmaceutical Sciences, southwest university, chongqing 400715, china key laboratory on Luminescence Real-Time Analysis Ministry of Education Southwest University Chongqing China college of Life Science Southwest University Chongqing China college of chemistry and chemical engineering Southwest University Chongqing China college of Pharmaceutical Sciences Southwest University Chongqing China

Homochirality in life has always been a driving force in scientific research and natural exploration. It has not been satisfactorily explained, and systematic investigations are necessary. This paper reported a homochiral expression of proteins dependent on the stirring direction of growing media. By controlling the stirring direction clockwise (CW) and anticlockwise (ACW) of the culture medium, proteins with distinct secondary structures were obtained, and D-amino acid may be included in the protein cultured with the stirring direction of ACW. Considering the effect of force fields, which might affect the process of folding and refolding of cellular protein in this report, the control of force fields might be a good way to prepare asymmetric drugs, and the rotational direction of the earth is possibly related to the chirality in primitive life molecules.

关键词： homochirality chiral expression prion protein fluorescence

Tungstate ion resonance Rayleigh scattering method for determination of basic triphenylmethane dyes

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Chinese Science Bulletin 2010年第8期55卷 693-700页

作者： YU LiHong,LIU ZhongFang,HU XiaoLi,KONG Ling & LIU ShaoPu School of chemistry and chemical engineering key laboratory on Luminescence and Real-Time Analysis,ministry of education,southwest university,chongqing 400715,china School of chemistry and chemical engineering key laboratory on Luminescence and Real-Time Analysis Ministry of Education Southwest University Chongqing China

In a pH 4.2-6.0 HAc-NaAc buffer medium,the resonance Rayleigh scattering intensities of separate basic triphenylmethane dyes,such as methyl violet (MV),ethyl violet (EV),crystal violet (CV),methyl green (MeG),iodine green (IG),malachite green (MG),light green (LG),and tungstate ion solution,are very weak,but when these cationic dyes react with the tungstate ion (WO 42-) to form ion-association complexes,their RRS are enhanced sharply and new RRS spectra *** reaction products of methyl violet,ethyl violet,and crystal violet with WO 42-have similar spectral characteristics and there are two strong RRS peaks at 330 nm and 560 *** reaction products of methyl green,iodine green,malachite green,light green with WO 42-have similar spectral characteristics and there are three RRS peaks at about 335 nm,450 nm and 580 *** sensitivities of seven kinds of reaction products show a sequence of malachite green>light green>crystal violet>ethyl violet>methyl green>iodine green>methyl *** linear ranges and detection limits of the above seven systems are 0.06-2.78 μg mL-1 and 18.1 ng mL-1 (MG),0.08-4.83 μg mL-1 and 22.6 ng mL-1 (BG),0.10-4.90 μg mL-1 and 31.4 ng mL-1 (CV),0.14-2.95 μg mL-1 and 40.5 ng mL-1 (EV),0.17-3.70 μg mL-1 and 50.2 ng mL-1 (MeG),0.22-5.70 μg mL-1 and 67.1 ng mL-1 (IG),and 0.25-7.30 μg mL-1 and 76.3 ng mL-1 (MV).A sensitive,simple and fast RRS method for the determination of basic triphenylmethane dyes using the tungstate ion as the probe has been *** method is applicable to the determination of malachite green residues in fish flesh.

关键词：三苯甲烷光谱法钨离子染料测定散射瑞利共振

The resonance Rayleigh scattering of the interaction of copper(Ⅱ)-bleomycinA2 with DNA and its analytical application

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Science china chemistry 2010年第3期53卷 618-624页

作者： LIU JiangTao1,LIU ShaoPu2 & LIU ZhongFang2 1School of chemistry,chongqing Normal university,chongqing 400300,china 2School of chemistry and chemical engineering,southwest university,chongqing 400715,china School of chemistry Chongqing Normal University Chongqing China School of chemistry and chemical engineering Southwest University Chongqing China

The forming of bleomycinA2-Cu(Ⅱ) cationic chelate and the interaction of the chelate with DNA have been investigated by using resonance Rayleigh scattering(RRS),molecular absorption and fluorescence *** result shows that in aqueous solution,bleomycinA2(BLMA2) can react with Cu(Ⅱ) to form 1:1 cationic chelate which contributes to the changes of the absorption spectra and the quenched fluorescence of *** the cationic chelate further bound with DNA to form ternary ion-association complexes,the remarkable enhancement of the RRS intensity was *** this work,the optimum conditions for the coordination reaction of BLMA2 with Cu(Ⅱ) and some influencing factors have been *** reaction mechanism of BLMA2-Cu(Ⅱ) binding with DNA was suggested and a binding model was *** addition,the fluorescence quenching type of BLMA2 was investigated.A highly sensitive,simple and rapid new method for the determination of DNA by using BLMA2-Cu(Ⅱ) as RRS probe has been *** detection limits(3σ) are 7.2 ng/mL for ctDNA,7.1 ng/mL for sDNA and 18 ng/mL for *** method can be applied to the determination of trace amounts of DNA.

关键词： bleomycinA2 Cu(Ⅱ) DNA resonance Rayleigh scattering fluorescence quenching

Improved electrochemical performances of polyaniline nanotubes-poly-L-lysine composite for label-free impedance detection of DNA hybridization

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Science china chemistry 2010年第6期53卷 1371-1377页

作者： YANG Tao,JIANG Chen,ZHANG Wei & JIAO Kui key laboratory of Eco-chemical engineering,ministry of education college of chemistry and Molecular engineering,Qingdao university of Science and technology,Qingdao 266042,china key laboratory of Eco-chemical engineering Ministry of Education college of chemistry and Molecular engineering Qingdao University of Science and Technology Qingdao China

A sensitive label-free DNA hybridization biosensing platform was fabricated based on the synergistic effect of polyaniline nanotubes (PANInt) and poly-L-lysine (pLys).The composite of pLys and PANInt was coated onto the carbon paste electrode (CPE) to form a uniform and very stable nanocomposite *** pLys in the composite film not only acts as a membrane to retain good electron transfer capability of PANInt even at physiological pH,but also possesses fine biocompatibility for *** probes with negatively charged phosphate groups were readily linked to the positively charged pLys surface due to the strong electrostatic *** synergistic effect of PANInt and pLys could significantly enhance the sensitivity of DNA hybridization *** phosphinothricin acetyltransferase (PAT) gene fragment from transgenic corn and the polymerase chain reaction amplification of the terminator of nopaline synthase gene from the real sample of a kind of transgenic soybean were detected by this DNA electrochemical biosensor via label-free impedance *** stable composite gives convenient permselectivity properties as a transducer material for the design of modern electrochemical impedance biosensor using [Fe(CN)6]3-/4as an indicator.

关键词： poly-L-lysine polyaniline nanotubes DNA electrochemical biosensor PAT gene NOS gene