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Electrophoretic Coating of Hydroxyapatite on Pyrolytic Carbon Using Glycol as Dispersion Medium

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Journal of Wuhan University of technology(Materials science) 2008年第3期23卷 293-297页

作者：高林 college of life science and technology Huazhong University of Science and Technology college of Chemistry and Environmental Engineering Yangtze UniversityJingzhou 434023China

Hydroxyapatite （HA） coatings on pyrolytic carbon were produced via electrophoretic deposition （EPD） using glycol and ethanol as dispersion medium respectively. The effect of the solubility of HA in the dispersion medium on crack occurrence and adherence of the coating was investigated by means of scanning electronic microscope （SEM） and atomic absorption spectrometer （AAS）. The results show that the solubility of HA in glycol is higher than that in ethanol. The usage of glycol as a dispersion medium can reduce the possibility of crack formation and enhance the adhesive strength between the coating and the carbon substrate. The green coatings can be sintered under vacuum at 1 000 ℃ whether the coatings are obtained using ethanol or glycol as dispersion medium. No HA decomposition was observed up to 1 000 ℃ by vacuum sintering by X-ray diffraction （XRD） analysis.

关键词： hydroxyapatite electrophoretic deposition coatings dispersion medium glycol

Improving DNA damage repair ability of E. coli to UV-radiosensitivity by DNA-mediated gene transfer of low energy ion beam

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Chinese science Bulletin 1999年第19期44卷 1778-1783页

作者： SONG Daojun and YU XunCenter of Ion Beam Bioengineering, Institute of Plasma Physics , Chinese Academy of sciences , Hefei 230031, China college of life science , University of science and technology of China , Hefei 230026, China Center of Ion Beam Bioengineering Institute of Plasma Physics Chinese Academy of Sciences Hefei China college of life science University of Science and Technology of China Hefei China

Using calcium chloride method of transfer gene as control, a new technique of transferring gene by low energy ion beam has been applied to the study of improving DMA damage repair ability of E. coli to UV-radiosensitivity. The genome DMA pieces of Deinococcus radio-durans, as 'foreign' genetic materials, were introduced into the UV-radiosensitive strains of E.

关键词： ion implantation Deinococcus radiodurans transfer gene survival rate unscheduled DNA synthesis.

Mutations in β-G spectrin disrupt the DCVs excytosis in C. elegans

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生物物理学报 2009年第S1期25卷 210-211页

作者： Ya-Ming Jiu, Xian-Guang Lin, Jun-Wei Yu, Zheng-Xing Wu college of life science and technology, Huazhong University of science and technology, Wuhan 430074, P.R.China

β spectrin is a major component of the membrane skeleton, a structure found at the plasma membrane of most animal cells. In C. elegans, the genome encodes only one

关键词： C.elegans β-G spectrin excytosis DCVs

Interaction of Mouse Pem Protein and Cell Division Cycle 37 Homolog

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Acta Biochimica et Biophysica Sinica 2005年第11期37卷 784-787页

作者： Fen GUO Yue-Qin LI Shi-Qian LI Zhi-Wen LUO Xin ZHANG Dong-Sheng TANG Tian-Hong ZHOU college of life science and technology,Jinan University.Guangzhou 510632.China collegeoflifescienceandtechnology JinanUniversityGuangzhou510632China

Mouse Pem,a homeobox gene,encodes a protein consisting of 210 amino acid *** the function of mouse Pem protein,we used the yeast two-hybrid system to screen the library of 7-daymouse embryo with full-length mouse Peru ***-two colonies were obtained after 1.57×10~8 colonieswere screened by nutrition limitation and β-galactosidase *** individual insert fragments wereobtained from the library,and three of them were identified,one of which was confirmed to be the celldivision cycle 37(Cdc37)homolog gene by *** interaction between mouse Pem and Cdc37homolog was then confirmed by glutathione S-transferase pull-down assay,and the possible interaction modelwas suggested.

关键词： mPem cell division cycle 37 homolog yeast two-hybrid system

Large-scale information entropy analysis of important sites in mature and precursor miRNA sequences

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science China(life sciences) 2009年第8期52卷 771-779页

作者： DU JiangFeng1,2,WU YongJun2,ZHANG YinXia3,WU Lei1,2,WANG XiaoLong3,TAO ShiHeng1,2 1 Bioinformatics Center,Northwest science and technology University of Agriculture,Yangling 712100,China 2 college of life sciences,Northwest science and technology University,Yangling 712100,China 3 college of science,Northwest science and technology University,Yangling 712100,China Bioinformatics Center Northwest Science and Technology University of Agriculture Yangling China college of life sciences Northwest Science and Technology University Yangling China college of science Northwest Science and Technology University Yangling China

In order to find evidence of consistent sequence conservation or the base correlation degree in miRNA,some important sites in the sequences of reported miRNA and their precursors(pre-miRNA) were investigated via information entropy *** different groups of sites were obtained from special locations(head,tail) in miRNAs of different sources according to taxonomy(animal,plant and virus) and then analyzed by measuring the single base information redundancy(D1(L)) and the adjacent base related information redundancy(D2(L)).The results showed that D2(L) has more information than D1(L),though D1(L) changes roughly consistently with D2(L) in each *** pre-miRNAs are more conservative than those belonging to animals or *** addition,U is dominant in most sites compared with other *** was also found that in the middle of several groups,there were sites where miRNAs were cut down from pre-miRNAs by Enzyme Dicer which were significantly *** phenomenon shows that the conservatism is an aspect of the of miRNA and may be involved in the recognition and cutting by the *** results provided another perspective for understanding more about the primary structure of pre-miRNA.

关键词： miRNA conservative information entropy precursor evolution base correlation degree

PEGylated PLGA Nanoparticles as Tumor Ecrosis Factor-α Receptor Blocking Peptide Carriers:Preparation,Characterization and Release in vitro

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Journal of Wuhan University of technology(Materials science) 2007年第1期22卷 112-116页

作者：刘卫杨祥良 college of life science and technology Huazhong University of Science and Technology

To assess the merits of PEGylated poly （lactic-co-glycolic acid）（PEG-PLGA） nanoparticles as drug carriers for tumor necrosis factor-α receptor blocking peptide （TNFR-BP）, PEG-PLGA copolymer, which could be used to prepare the stealth nanoparticles, was synthesized with methoxypolyethyleneglycol, DL-lactide and glycolide. The structure of PEG-PLGA was confirmed with ^1H-NMR and FT-IR spectroscopy, and the molecular weight （MW） was determined by gel permeation chromatography. Fluorescent FITC-TNFR- BP was chosen as model protein and encapsulated within PEG-PLGA nanoparticles using the double emulsion method. Atomic force microscopy and photon correlation spectroscopy were employed to characterize the stealth nanoparticles fabricated for morphology, size with polydispersity index and zeta potential. Encapsulation efficiency （EE） and the release of FITC-TNFR-BP in nanopartieles in vitro were measured by the fluorescence measurement. The stealth nanoparticles were found to have the mean diameter less than 270 nm and zeta potential less than -20 mV. In all nanoparticle formulations, more than 45% of EE were obtained. FITC-TNFR-BP release from the PEG-PLGA nanoparticles exhibited a biphasic pattern, initial burst release and consequently sustained release. The experimental results show that PEG-PLGA nanoparticles possess the potential to develop as drug carriers for controlled release applications of TNFR-BP.

关键词： tumor necrosis factor-α receptor blocking peptide PEG-PLGA stealth nanoparticles ring-opening polymerization controlled and sustained drug release

In Vitro Construction of Effective M1GS Ribozymes Targeting HCMV UL54 RNA Segments

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Acta Biochimica et Biophysica Sinica 2005年第3期37卷 210-214页

作者： Yun-Zhen SU Hong-Jian LI Yue-Qin LI Hao-Jun CHEN Dong-Sheng TANG Xin ZHANG Hong JIANG Tian-Hong ZHOU college of life science and technology,Jinan University,Guangzhou 510632,China collegeoflifescienceandtechnology JinanUniversityGuangzhou510632China

Seven sequence-specific ribozymes(M1GS RNAs)derived in vitro from the catalytic RNAsubunit of Escherichia coli RNase P and targeting the mRNAs transcribed by the UL54 gene encoding theDNA polymerase of human cytomegalovirus were screened from 11 ribozymes that were designed based onfour rules:(1)the NCCA-3′ terminal must be unpaired with the substrate;(2)the guide sequence(GS)mustbe at least 12 nt in length;(3)the eighth nucleotide must be U,counting from the site-1;and(4)around thecleavage site,the sites-1/+1/+2 must be U/G/C or C/G/*** investigation of the factors affecting thecleavage effect and the optimal ratio for M1GS/substrate was carried *** was determined that the optimalratio for M1GS/substrate was 2:1 and too much M1GS 1ed to substrate *** indicated *** M1GS that cleaved HCMV UL54 RNA segments in vitro were successfully designed and *** studies support the use of ribozyme M1GS as antisense molecules to silence HCMV mRNA in vitro,andusing the selection procedure as a general approach for the engineering of RNase P ribozymes.

关键词： ribonuclease P (RNase P) guide sequence HCMV DNA polymerase

Overview of Rim2/Hipa transposon superfamily:Structure, distribution, transposition and utilization

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Progress in Natural science:Materials International 2008年第4期18卷 375-379页

作者： Pingfang Tian a, Zuhua He b a college of life science and technology, Beijing University of Chemical technology, Beijing 100029, China b Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological sciences, Chinese Academy of sciences, Shanghai 200032, China college of life science and technology Beijing University of Chemical Technology Beijing 100029 China Shanghai Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The rice Rim2/Hipa is a unique transposon superfamily in Oryza genomes, which can be transcriptionally activated by the infection of the rice blast pathogen Magnaporthe grisea and its elicitors. Rim2/Hipa elements belong to En/Spm family, with perfect terminal inverted repeats in both ends, direct and inverted sub-terminal repeats, and 3-bp target site duplication upon insertion. Intriguingly, the typical ’CACTG’ end sequences in the terminal inverted repeats of most Rim2/Hipa elements distinguish them from previously reported CACTA elements, such as En/Spm, Tam1, and Tgm1, which harbor ’CACTA’ sequence in their terminal inverted repeats. In particular, the plentiful Rim2/Hipa elements unevenly distribute over all 12 chromosomes and make up at least 1% of the entire genome. Southern hybridization revealed the existence of Rim2/Hipa elements in all rice varieties examined and the Rim2/Hipa core sequence is unique to rice genomes. Evidence derived from sequence analysis indicated the occurrence of transposition events during the long evolution. The abundance and insertion polymorphisms suggested their applications as novel molecular marker in rice fingerprinting and fine chromo- some mapping. This paper aims at outlining the Rim2/Hipa elements with respect to their structure, distribution, transposition and utilization.

关键词： Rim2/Hipa elements Structure Distribution Transposition Utilization

EMMA:An Efficient Massive Mapping Algorithm Using Improved Approximate Mapping Filtering

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 857-864页

作者： Xin ZHANG~1 Zhi-Wei CAO~2 Zhi-Xin LIN~3 Qing-Kang WANG~1* Yi-Xue LI~4 1 Institute of Micro/Nano science and technology,Shanghai Jiaotong University,Shanghai 200030,China 2 Shanghai Center for Bioinformation technology,Shanghai 200235,China 3 college of life science and technology,Shanghai Jiaotong University,Shanghai 200030,China 4 Bioinformation Center of Shanghai Institutes for Biological sciences,Chinese Academy of sciences,Shanghai 200031,China Shanghai Center for Bioinformation technology Shanghai 200235China Institute of Micro/Nano science and technology Shanghai Jiaotong UniversityShanghai 200030China

Efficient massive mapping algorithm (EMMA),an algorithm on efficiently mapping massivecDNAs onto genomic sequences,has recently been *** process of mapping massive cDNAsonto genomic sequences has been improved using more approximate mapping filtering based on an enhancedsuffix array coupled with a pruned fast hash table,algorithms of block alignment extensions,and *** compared with the classical BLAT software in this field,the computing of EMMA ranges fromtwo to forty-one times faster under similar prediction precisions.

关键词： cDNA mapping maximal exact match enhanced suffix array pruned fast hash table extension algorithm