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Characterization of Function of Three Domains in Dishevelled-1: DEP Domain is Responsible for Membrane Translocation of Dishevelled-1

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Cell Research 2004年第4期14卷 324-330页

作者： Wei Jun PAN, Shu Zhao PANG, Tao HUANG, Hui Yun GUO, Dianqing WU, Lin LI Laboratory of Molecular Cell biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for Biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, 320 Yueyang Rd, Shanghai 200031 college of life science, jilin university, changchun 130061, china. department of genetics and developmental biology, university of connecticut health center, farmington, connecticut 06030, usa. Laboratory of Molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai college of life science Jilin University Changchun China department of genetics and developmental biology University of Connecticut Health Center Farmington Connecticut USA

Wnt signaling plays an important role in embryogenesis and tumorgenesis. Although the mechanism about how Wnts transduce their signaling from receptor frizzled (Fz) to cytosol has not been understood, dishevelled (Dvl) protein was considered as the intersection of Wnt signal traffic. In this study, we characterized the function of three domains (DIX, PDZ and DEP) of Dvl-1 in canonical Wnt signal transduction and Dvl-1 membrane translocation. It was found both DIX and DEP domain were sufficient to block Wnt-3a-induced LEF-1 transcriptional activity and free cytosol β-catenin accumulation; whereas PDZ domain and a functional mutant form of DEP domain (DEP-KM) had no effect on canonical Wnt signaling. In addition, when cotransfected with Fz-7, DEP domain, but not DIX, PDZ or DEP-KM, translocated and co-localized with Fz-7 to the plasma membrane, which was similar to Dvl-1. Furthermore, it was DEP domain that could block Fz-7-induced membrane translocation of Dvl-1 via a possible competitive mechanism. Thes

关键词： Wnt frizzled-7 dishevelled DIX PDZ DEP membrane translocation signal transduction

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Development of rabbit monoclonal and polyclonal antibodies for detection of site-specific histone modifications and their application in analyzing overall modification levels

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Cell Research 2006年第5期16卷 519-527页

作者： Lan Guo~(1,2,*) Benliang Yin~(1,*) Junli Zhou~(1,3) Xueyong Li~3 Xing Wang Deng~(1,2,3) ~1National Institute of Biological science,Zhongguancun life science Park,Beijing 102206,china.~2Peking-Yale Joint center of Plant Molecular genetics and Agrobiotechnology,college of life sciences,Peking university,Beijing 100871,china.~3department of Molecular,Cellular,and developmental biology,Yale university,New Haven,connecticut 06520-8104,usa National Institute of Biological science Zhongguancun Life Science Park Beijing China Peking-Yale Joint center of Plant Molecular genetics and Agrobiotechnology College of Life Sciences Peking University Beijing China department of Molecular Cellular and Developmental Biology Yale University New Haven USA

In addition to DNA sequence information,site-specific histone modifications are another important determinant ofgene expression in a eukaryotic *** selected four modification sites in common histones that are known tosignificantly impact chromatin function and generated monoclonal or polyclonal antibodies that recognize each of thosesite-specific *** used these antibodies to demonstrate that the site-specific histone modification levelsremain relatively constant in different organs of the same *** also compared the levels of selected histonemodifications among several representative organisms and found that site-specific modifications are highly variable amongdifferent organisms,providing new insight into the evolutionary divergence of specific histone modifications.

关键词： histone modification site-specific antibody chromatin

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