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生理学报 2019年第4期71卷 581-587页

作者：丁惠洁刘珂舟杨珊珊李鹏严明杭州电子科技大学生命信息与仪器工程学院杭州310018 浙江大学光学科学与工程学院杭州310027 浙江大学生物医学工程与仪器科学学院杭州310027

本研究旨在建立观察脑皮层血管堵塞、脑缺血损伤区血管再生、重构的动态变化过程的方法。创建能够在体连续观测大鼠皮层血管缺血后变化的动物光窗模型,并用光化学栓塞法建立大鼠皮层局部脑缺血模型,通过光相干层析成像术(optical cohere... 详细信息

本研究旨在建立观察脑皮层血管堵塞、脑缺血损伤区血管再生、重构的动态变化过程的方法。创建能够在体连续观测大鼠皮层血管缺血后变化的动物光窗模型,并用光化学栓塞法建立大鼠皮层局部脑缺血模型,通过光相干层析成像术(optical coherence tomography, OCT)实时记录血管堵塞的形成过程以及周围小血管的损伤、再生情况。结果显示,激光照射30 min可以形成完全堵塞大鼠皮层血管的栓塞;缺血后24~48 h内,脑损伤程度最大,损伤区域内血管数量达到最少,随后堵塞的主血管开始疏通,缺血区域内周围小血管开始再生;激光照射后皮层浅层/深层的小血管显著消失,在照射后第10天,皮层浅层出现大量新生血管,而深层血管仍未恢复。以上结果表明,本研究建立的方法可以进行不间断地长期观测,为深入定量研究脑缺血恢复期间栓塞血管及周边毛细血管的动态奠定了基础,为损伤区血管重构网络调控模式研究提供帮助,为脑缺血性疾病的治疗提供理论指导和新研究方法。

关键词：光化学栓塞法局部脑缺血光相干层析成像血管再生

Nitric Oxide Inducing Function and Intracellular Movement of Chicken Interleukin-18 in Cultured Cells

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 688-693页

作者： Jian XU~(1,3) Tong-Le DENG~2 Long LI~1 Zhen-Qiang YOU~1 Wang-Jun WAN~2 Lian YU~(1*) 1 Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine.Institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029.China 2 college of biomedical engineering and instrument science,Zhejiang University,Hangzhou 310029,China 3 Medical college of Shihezi University,Xinjiang 832002,China Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine Institute of Preventive Veterinary Medicine Zhejiang University Hangzhou 310029 China

To evaluate the characteristics of chicken interleukin-18 (ChIL-18) in different forms in vitro,the ChIL-18 full-length gene (ChIL-18-F) and the ChIL-18 presumed mature protein gene (ChIL-18-M) werecloned and inserted into the eukaryotic expression vector pCI,to construct recombinant pCI-ChIL-18-Fand *** recombinant plasmids were then transferred into chicken splenic lymphocytes(CSLs).Western blot showed that ChIL-18-F,with a molecular weight of 23.0 kDa,was produced in CSLstransfected by pCI-ChlL-18-F;ChIL-18-M,with a molecular weight of 19.5 kDa,was produced in CSLstransfected by *** nitric oxide (NO) level in the transfected CSLs and the culture mediumat different time points was further examined under confocal microscopy using *** results showed that both pCI-ChIL-18-F and pCI-ChIL-18-M groups showed significantincrease in intracellular and extracellular NO production compared with pCI transfected control *** suggest that both ChIL-18-F and ChIL-18-M could stimulate NO secretion in *** characterizethe intracellular distribution of ChIL-18,ChIL-18-F and ChIL-18-M were each fused to the enhanced greenfluorescent protein gene,and expressed in Vero *** results showed that the ChIL-18-F tended to themembranous region in Vero cells,while ChIL-18-M did *** indicates that the N-terminal 27 amino acidpeptide helped ChIL-18 target to Veto cell membranes.

关键词： chicken interleukin-18 N-terminal peptide nitric oxide splenic lymphocyte intracellular movement

A Novel Method of Signal Enhancement Based on Modified FastICA Algorithm

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A Novel Method of Signal Enhancement Based on Modified FastI...

Influence of the MFB stimulation on the mesencephalic dopaminergic system

ICEMI'2005第七届国际电子测量与仪器学术会议

作者： Wu Lili1 Chen Yuquan1 Huang Pinggao2 Mao Yuru3 Zhang Baojun4 (1State Specialized Laboratory of biomedical Sensors college of biomedical engineering and instrument science Zhejiang University Hangzhou 310027 PR China (2Guilin University of electronic Technology Guilin 541004 China) (3Chinese Research Academy of Environmental sciences Beijing 100012 China) (4college of Computer science Zhejiang University Hangzhou 310027 China)

Independent component analysis (ICA) has attracted much attention in the field of signal processing. A novel method of speech signal enhancement by using modified FastICA algorithm is presented in thi

关键词： Independent component analysis (ICA) modified FastICA algorithm signal enhancement Newton iteration.

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Influence of the MFB stimulation on the mesencephalic dopami...

葡萄糖氧化酶传感器中酶的固定与电子转移研究进展

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Qian LI~(1,2),Shao-Min ZHANG~(1,2,*),Yi YU~(1,2),Huai-Jian ZHANG~(1,2),Qiao-Sheng ZHANG~(1,2),Xiao-Xiang ZHENG~(1,2) 1 Qiushi Academy for Advanced Studies,Zhejiang University,Hangzhou 310027,China 2 college of biomedical engineering and instrument science,Zhejiang University,Hangzhou 310027,China

＜正＞Objective As the main component of brain’s reward center,the electrical stimulation of the medial forebrain bundle（MFB） leads to the activation of the mesocorticolimbic dopaminergic *** effect elicits the intense intracranial self-stimulation（ICSS）,which strengthens the rats to perform a series of reward-motivated reinforcement *** the MFB is assembled by the axon bundles of dopaminergic neurons both in the mesocorticolimbic pathway and nigrostriatal pathway,the present study aims to explore that the MFB stimulation not only activates the brain’s reward center,but also impacts the nigrostriatal pathway,which participates the cortico-basal ganglia *** 18 adult male Sprague-Dawley rats were randomly divided into GroupsⅠ,ⅡandⅢ.Group I:stimulating electrode was implanted into hibateral MFB（Aβ:-3.60;ML:±1.80;DV:+9.20）.By using an eight-arm radial maze,the rat’s preferable turning direction was recorded when the stimulation was delivered in the arm exit,which reliably reflected the different effects of MFB stimulation on the locomotor behavior of bilateral *** II:stimulating electrode and single-channel local field potential（LFP） recording electrode were implanted into the bilateral MFB and the primary cerebral cortex（M1, Aβ:+2.50;ML:±3.00;DV:+1.20）.After recording the Ml-LFPs pre and post stimulation,wavelet packet transform（WPT） was applied to make time-frequency spectrum ***Ⅲ:stimulating electrode and 7-channel spike recording bundle were implanted into the unilateral MFB and the ipsilateral entopeduncular nucleus（EP,Aβ:-2.40;ML:±2.80;DV: +7.60）.After getting the EP-spikes pre and post stimulation,detecting and sorting algorithm helped to draw each neuron’s ***-Stimulus Time Histogram（PSTH） and Inter-Spike Intervals（ISIs） were plotted to decode the firing patterns of the EP-GABAergic ***（1）Unilateral MFB stimulation caused a striking asymmetry in the rat’s locomotor *** contralateral turning w

关键词： medical forebrain bundle mesocorticolimbic dopaminergic pathway nigrostriatal pathway basal ganglia extrapyramidal system

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生物医学工程与临床 2009年第4期13卷 366-369页

作者：潘晓东刘枫解放军第117医院浙江杭州310013 浙江大学生物医学工程与仪器科学学院浙江杭州310013

回顾了葡萄糖氧化酶(GOD)传感器研究的新进展。介绍了GOD的固定(包括物理吸附、化学偶联、使用载体等方法的研究进展)、电子的转移(使用碳纳米管、纳米颗粒等方法的研究进展),并对GOD传感器的发展前景进行了预测。

关键词：葡萄糖氧化酶酶传感器酶的固定电子的转移碳纳米颗粒碳纳米管

The Detection of Defects in Optical Fibers Using a Hybrid Opto-electronic Correlator

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Chinese Journal of Lasers 2000年第3期9卷 15-21页

作者： LIU Yange LIU Wei ZHANG Yimo ZHOU Ge (college of Precision instrument and Opto-electronics engineering, Tianjin University Opto-electronic Information science and Technology Lab., Tianjin 300072, China) 天津大学光电信息技术科学开放实验室天津

A hybrid opto-electronic correlator for detecting defects i n optical fibers is proposed. After the light from a He-Ne laser being expanded and filtered it is not collimated but directly passes a Fourier transform lens and illuminates a reference fiber and a test fiber at the same input plane. The Fourier transform spectrum of the two fibers is therefore obtained at the rear focal plane of the lens, where it is sampled via a CCD array connected with a computer through a frame grabber. The computer performs filter, inverse Fourier transform and setting threshold operation on classification. The system is an equivalent of joint transform correlator with a Fourier lens of long focal length. The experiment results for optical fibers having incoordinate defects are presented. The results indicate that the system can be used for fiber defect detection, and has the advantages of high identification, compact configuration, easy adjustment and flexible manipulation.

关键词： joint transform correlation Fourier transform optical fiber defect detection

Positive Effect of Noises on Sensory Systems

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Journal of Bionic engineering 2004年第1期1卷 61-65页

作者： Jun Liu, Guang Li Institute of biomedical engineering and instrument science, Zhejiang University, Hangzhou 310027,P.R.China Zhejiang University Zhejiang University

Stochastic resonance phenomenon in the biological sensory systems has been studied through the signal detection theories and the psychophysical experiments. In this paper, sensory systems are considered as a threshold detector including the receiver part and the classifier part. Compared with conventional models regarding the receiver part of sensory system as a linear or single non-linear system, a summing network was constructed by MacCulloch-Pitts neurons to simulate the receiver part. The simulation results show that the relevant index of the detectability of signal exhibit the stochastic resonance behaviours. The psychophysical experiments were carried out through the 2IFC (two interval two alternative forced choice) method. The experimental results qualitatively verify the conclusion in accordance with the theoretical model. These works give a proof that stochastic resonance is not only epiphenonmenon in sensory systems.

关键词： stochastic resonance signal detection theory psychophysical experiment MacCulloch-Pitts neuron

Design and Implementation of a Virtual Multi-channel Optical Ring

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Chinese Journal of Lasers 2001年第6期10卷 12-17页

作者： JING Wencai ZHOU Ge ZHANG Yimo LI Haifeng ZHANG Hongxia (college of Precision instrument & Opto-electronics engineering, Tianjin University, Key Laboratory of Optoelectronics Information Technical science, EMC, Tianjin 300072, China) 天津大学精密仪器与光电子工程学院教育部光电信息技术科学重点实验室天津 300072

The hardware and the communication protocol of a scalable optical ring have been designed. A hardware routing has been achieved on the network interface card to minimize data packet communication latency and a simplified communication protocol has been designed to reduce communication overheads. To reduce the data packet blocking probability and have a more efficient usage of the data link layer bandwidth, a virtual multi-channel transmission mechanism has been achieved. The network supports a maximum of 16 virtual channels for each physical data link. Time slots can be dynamically assigned to each virtual channel. Zero wait time has been achieved for the switching between different virtual channels.

关键词： communication protocol scalable optical ring hardware routing virtual multi-channel transmission time slot

Forecasting Loss of Ecosystem Service Value Using a BP Network: A Case Study of the Impact of the South-to-north Water Transfer Project on the Ecological Environmental in Xiangfan, Hubei Province, China

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biomedical and Environmental sciences 2003年第4期16卷 379-391页

作者： YUN-FENG CHEN, JING-XUAN ZHOU, JIE XIAO, AND YAN-PING LIEnvironmental science and engineering college, Huazhong University of science and Technology, Wuhan 430074, Hubei, China Environmental science and engineering college Huazhong University of Science and Technology Wuhan 430074 Hubei Chin

Objective To recognize and assess the impact of the South-to-north Water Transfer Project (SNWTP) on the ecological environment of Xiangfan, Hubei Province, situated in the water-out area, and develop sound scientific countermeasures. Methods A three-layer BP network was built to simulate topology and process of the eco-economy system of Xiangfan. Historical data of ecological environmental factors and socio-economic factors as inputs, and corresponding historical data of ecosystem service value (ESV) and GDP as target outputs, were presented to train and test the network. When predicted input data after 2001 were presented to trained network as generalization sets, ESVs and GDPs of 2002, 2003, 2004... till 2050 were simulated as output in succession. Results Up to 2050, the area would have suffered an accumulative total ESV loss of RMB 104.9 billion, which accounted for 37.36% of the present ESV. The coinstantaneous GDP would change asynchronously with ESV, it would go through an up-to-down process and finally lose RMB89.3 billion, which accounted for 18.71% of 2001. Conclusions The simulation indicates that ESV loss means damage to the capability of socio-economic sustainable development, and suggests that artificial neural networks (ANNs) provide a feasible and effective method and have an important potential in ESV modeling.

关键词： Artificial neural network BP Ecosystem service value South-to-north Water Transfer Project