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A taxonomic re-assessment in the Chinese Bupleurum(Apiaceae):Insights from morphology,nuclear ribosomal internal transcribed spacer,and chloroplast(trnH-psbA,matK) sequences

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Journal of Systematics and Evolution 2011年第6期49卷 558-589页

作者： Chang-Bao WANG Xiang-Guang MA Xing-Jin HE(college of life science,Sichuan University,Chengdu 610064,China) (college of life science Sichuan University Chengdu 610064 China) *Correspondence: Xing-Jin HE *Correspondence: E-mail: xjhe@*** Tel.: 86-28-85415006 Fax: 86-28-85415006.

As one of the big genera in Apiaceae,Bupleurum *** received considerable attention from plant ***,there are still many gaps in our knowledge of this genus,especially of those species endemic to China that have often been excluded from previous *** spite of a few recent studies on the phylogeny and classification of the Chinese Bupleurum,the most essential problem of species delimitation still remains *** re-evaluate the taxonomy of the Chinese Bupleurum using morphological and molecular *** observations of living plants growing the field and herbarium specimens were made to understand the morphological variation and to ensure species identification for the molecular *** analyses were carried out on sequence data of nuclear ribosomal internal transcribed spacer(nrITS),trnH-psbA,and matK,using parsimony and Bayesian approaches to evaluate species *** of both nrITS and combined datasets of the three regions revealed the following results:(i)the Chinese Bupleurum species were divided into two major lineages;and(ii)most species were well defined,but some traditional inter-and infraspecific relationships were not *** results,along with the results of previous works support that the Chinese Bupleurum species were derived from two different lineages and should be placed in Neves and Watson’s subgenus *** detailed taxonomic re-assessment of several Chinese species resulted in new taxonomic recombinations with rank changes for three species:Bupleurum tenerum,***,and ***;new combination for one variety,*** ***;and the description of one variety and one species,*** *** and ***.

关键词： Chinese Bupleurum chloroplast sequences(trnH-psbA matK) morphology nrITS phylogenetic analyses taxonomy and new species.

New fossil elaterid (Coleoptera: Polyphaga: Elateridae) from Yixian Formation of western Liaoning, China

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Progress in Natural science:Materials International 2007年第10期17卷 1244-1249页

作者： Chang Huali, Ren Dong and Shih Chungkun(college of life science, Capital Normal University, Beijing 100037, China) college of life science Capital Normal University Beijing 100037 China

A new species of the elaterid genus Cryptocoelus Dolin and Nel, 2002 from the Late Jurassic to Early Cretaceous Yixian Formation in the western Liaoning, China is described. Diagnosis of the genus is revised, and the systematic position of the genus is briefly discussed. Because the elaterids originated in the Early to Mid-Jurassic, this new material from the Late Jurassic-Early Cretaceous will enhance our understanding of the transition between ancient elaterids and extant ones, bridge the gap of cryptic relationships between the Mesozoic cupedids and elaterids, and expand our knowledge of their evolutionary history.

关键词： Elateridae new species Yixian Formation China.

A new fossil genus of Mesosciophilidae(Diptera,Nematocera) with two new species from the Middle Jurassic of Inner Mongolia,China

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Progress in Natural science:Materials International 2009年第12期19卷 1837-1841页

作者： Tingting Li,Dong Ren~* college of life science,Capital Normal University,Beijing 100048,China [a]college of life science Capital Normal University Beijing 100048 China

A new genus within the family Mesosciophilidae,Jurasciophila ***.,with two new species,J,curvula *** *** *** *** ***.,are described and *** are established based on fossil specimens with bodies and complete *** of them were collected from the late Middle Jurassic Jiulongshan Formation of Daohugou in southeastern Inner Mongolia,*** with two known genera,Mesosciophilina(Kovalev,1985) and Mesosciophilopsis(Blagoderov,1994),the new genus has transitional characters. The distribution pattern of Mesosciophilidae is briefly discussed.

关键词： Mesosciophilidae Middle Jurassic Jiulongshan Formation

pH-Dependent Stability of EGX, A Multi-functional Cellulase from Mollusca, Ampullaria crossean

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 603-608页

作者： Wen-Ying LI2, Ji WANG1, Yan-Hong LI1, Ming DING1, Gen-Jun XU1,3, Lan-Ying LIU2, and Fu-Kun ZHAO1,3* 1Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological sciences, the Chinese Academy of sciences,Graduate School of the Chinese Academy of sciences, Shanghai 200031, China 2college of life science, Jilin University, Changchun 130023, China college of life science, Zhejiang University of science and Technology, Hangzhou 310018, China Key Laboratory ofProteomics Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesGraduate School of the Chinese Academy of SciencesShanghai 200031China

The cellulase activity and stability of EGX, a multi-functional cellulase previously purified from the mollusca Ampullaria crossean, was systematically studied under different pH. The pH induced con- formation and stability change of EGX have been investigated by using the intrinsic fluorescence, ANS fluorescence and CD spectrum. It has been found that the conformation and activity of this cellulase were strongly dependent on the pH. EGX was stable for both the enzyme activity and the conformation from pH 5.6 to pH 7.4. As shown by intrinsic and ANS fluorescence, no red shift of emission maximum occurred and a negligible intensity change was observed at pH 5.6–7.4. The activity of EGX remained about 80% in pH 5.6–7.4 and obviously decreased out of side the pH range. Urea-induced changes in EGX at pH 5.4 and pH 8.0 were measured by intrinsic fluorescence and CD spectrum. At pH 5.4, a significantly red shift of emission maximum occurred when the concentration of urea was 5 M compared to the concentration was 3 M at pH 8.0. The α-helix at pH 5.4 was 40.51% in the absence of urea and 31.04% in the presence of 4 M urea. At pH 8.0 the α-helix was 7.23% in the presence of 4 M urea. The data indicated that EGX was much susceptible to urea-induced unfolding at pH 8.0 and much stable at pH 5.4. The greater pH dependent stability of EGX may allow the enzyme to adequately catalyze the hydrolysis of cellulosic materials under natural or industrial extreme conditions.

关键词： multi-functional cellulase stability Ampullaria crossean fluorescence spectroscopy pH-dependent structural change CD spectrum

Plant genomic DNA methylation in response to stresses: Potential applications and challenges in plant breeding

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Progress in Natural science:Materials International 2009年第9期19卷 1037-1045页

作者： Hai Peng, Jing Zhang * Laboratory of Germplasm and Genetics, college of life science, Jianghan University, Wuhan 430056, China [a]Laboratory of Germplasm and Genetics College of Life Science Jianghan University Wuhan 430056 China

The plant genome can respond rapidly and dynamically to stress in a manner that overcomes the restrictions of a highly stable DNA sequence. Abiotic stresses such as chilling, planting density, rubbing, cutting, and successive rounds of subculture generally decrease the levels of DNA methylation. The opposite effect is seen for salt stress, and the effects of heavy-metal stress are species specific. Biotic stres-ses such as pathogenic infection can lead to two contrasting effects on the levels of methylation in plants: hypermethylation on the genome-wide level and hypomethylation of resistance-related genes. Both phenomena may contribute to the adaptation of plants to stress. Although heritable methylation patterns and phenotypic variations that arise in response to stress are of potential value for plant breeding, their exploitation presents great challenges.

关键词： DNA methylation Plant breeding Stress

Two new Jurassic stoneflies (Insecta: Plecoptera) from Daohugou, Inner Mongolia, China

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Progress in Natural science:Materials International 2008年第8期18卷 1039-1042页

作者： Yushuang Liu, Dong Ren * college of life science, Capital Normal University, 105 Xisanhuanbeilu, Haidian District, Beijing 100037, China college of life science Capital Normal University 105 Xisanhuanbeilu Haidian District Beijing 100037 China

Two new genera and two new species of family Taeniopterygidae, Mengitaenioptera multiramis gen. et sp. nov. and Noviramonemoura trinervis gen. et sp. nov. are described. These fossils were collected from Daohugou Village (the Middle Jurassic), Inner Mongolia, China. Hitherto, these fossil species from Daohugou are the oldest taeniopterygids. Both simple and complex venations simultaneously occurred in this group, which reveals that the early diversification of taeniopterids was well underway by the Middle Jurassic. Therefore, we prefer to adopt the Comstock’s opinion that the venation of taeniopterids was derived from the simple venation of the older group (such as Palaeonemouridae) at much ancient geological time ago. Some of the descendants carried this simple venation; others evolved into complex venation with many additional crossveins and branches.

关键词： Plecoptera Taeniopterygidae Daohugou Inner Mongolia Middle Jurassic

A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A(CPA)

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Chinese Chemical Letters 1999年第2期10卷 121-122页

作者： Mei DU Sheng JIN Zhen Quan GUO(college of Chemistry and Molecular Engineering. Peking University. 100871)(college of life science, Peking University, 100871) Peking Univ Coll Chem & Mol Engn Beijing 100871 Peoples R China Peking Univ Coll Life Sci Beijing 100871 Peoples R China

The monoclonal antibody ID11D7 to carboxypeptidase A (CPA) was prepared. Theinhibitory effect of McAb ID11D7 on the peptidase activity of CPA was measured. The result reflectsthat the McAb ID11D7 can competitively inhibit the peptidase activity of CPA with an apparentinhibitory constant of 6.3 × 10-9M. Based on this work. the McAb ID11D7 can be used as idiotypicantigen to prepare anti-idiotypic catalytic antibody with the peptidase activity similar to CPA.

关键词： Monoclonal antibody carboxypeptidase A (CPA) competitive inhibition peptidaseactivity

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New fossil caddisfly from Middle Jurassic of Daohugou,Inner Mongolia,China(Trichoptera:Philopotamidae)

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Progress in Natural science:Materials International 2009年第10期19卷 1427-1431页

作者： Meixia Wang,Yunyun Zhao,Dong Ren college of life science,Capital Normal University,Beijing 100037,China [a]college of life science Capital Normal University Beijing 100037 China

A new genus and new species,Juraphilopotamus lubricus *** ***.,from the Middle Jurassic Jiulongshan Formation of Daohugou,Inner Mongolia,China,is described and *** may be the first record of the family Philopotamidae in China,extending the geographic distribution of this family.A detailed description and illustration of the specimen along with a brief review of the fossil Philopotamidae are given.A proposal on dispersionandmigration of Philopotamidae and problems of the paleoenvironment of the Daohugou beds are discussed.

关键词： Insecta Juraphilopotamus Philopotamidae Middle Jurassic Biogeography

New fossil elaterids(Insect:Coleoptera:Polyphaga:Elateridae) from the Middle Jurassic of Inner Mongolia,China

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Progress in Natural science:Materials International 2009年第10期19卷 1433-1437页

作者： Huali Chang,Yunyun Zhao,Dong Ren college of life science,Capital Normal University,Beijing 100048,China [a]college of life science Capital Normal University Beijing 100048 China

A new genus with a new species of fossil elaterid,Paraprotagrypnus superbus *** ***.,from the Middle Jurassic Jiulongshan Formation in Daohugou Village(N41°18.9790,E119°14.3180),Shantou Township,Ningcheng County,Inner Mongolia,China is described and *** genus Paraprotagrypnus belongs to the family of Elateridae,subfamily of Protagrypninae and tribe of *** primitive characters of the new genus and new species shed light on the ages between the Jiulongshan Formation in Inner Mongolia and the Yixian Formation in the western Liaoning Province of *** habitat of the new species is briefly discussed.

关键词： Elateridae New genus Paraprotagrypnus superbus sp.nov. Middle Jurassic Jiulongshan Formation China