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Screening and Phylogenetic Analysis of Deep-Sea Bacteria Capable of Metabolizing Lignin-Derived Aromatic Compounds

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Open Journal of Marine Science 2012年第4期2卷 177-187页

作者： Y. Ohta S. Nishi t. Haga t. tsubouchi R. Hasegawa M. konishi Y. Nagano Y. tsuruwaka Y. Shimane K. Mori K. Usui E. Suda K. tsutsui A. Nishimoto Y. Fujiwara t. Maruyama Y. Hatada Institute of Biogeosciences Japan Agency of Marine-Earth Science and Technology (JAMSTEC) Yokosuka Japan

Lignin is one of the most abundant biomasses in nature. It is composed of aromatic moieties and has great potential for use in the production of chemical alternatives to petroleum products. Because of increasing interest in biocatalysis, the potential for industrial application of microbial metabolism of lignin-derived compounds has gained considerable recent attention. Functional screenings of culturable bacteria isolated from sediments and sunken wood collected from the deep sea revealed the existence of a number of previously unidentified bacteria capable of metabolizing lignin-related aromatic compounds. Of the 510 isolates obtained in the present study, 208 completely or partially metabolized these compounds. the 208 isolates were classified into diverse phyla, including Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria. Among the 208 isolates, 61 unique 16S rRNA gene sequences were detected including previously unidentified marine lineage isolates. the metabolites of the isolates were analysed using liquid chromatography/mass spectrometry (LC/MS) or gas chromatography/mass spectrometry (GC/MS). Most of the representative 61 isolates non-oxidatively decarboxylated the substrates to produce the corresponding aromatic vinyl monomers, which are used as feed stocks for bio-based plastics production. Oxidative metabolism of the lignin-related compounds for assimilation was frequently observed. Our study showed that the deep-sea environment contains an abundance of microorganisms capable of both non-oxidative and oxidative bioconversion of lignin-derived aromatic compounds. the ability for bio-conversion of aromatic compounds found in this study will facilitate the development of future biotechnological applications.

关键词： Aromatic Compound Bacteria Deep Sea Lignin

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部署工业物联网(IIot)的关键考量

国内外机电一体化技术 2017年第1期20卷 13-13,15页

作者： colin Geis 美国红狮控制(中国)公司

＂我无法确保改变之后会变得更好;但若想变得更好,就必须改变。＂-Georg ***当今,研究工业物联网（IIo t）及阐述其能为工业组织（比如生产商和资源生产设施等）带来优势的文章不胜枚举,但其中较少涉及工业物联网（IIo t）技术的实际问... 详细信息

＂我无法确保改变之后会变得更好;但若想变得更好,就必须改变。＂-Georg ***当今,研究工业物联网（IIo t）及阐述其能为工业组织（比如生产商和资源生产设施等）带来优势的文章不胜枚举,但其中较少涉及工业物联网（IIo t）技术的实际问题并详细说明整个过程中的各种决策点。

关键词：工业组织物联网部署生产设施生产商决策点枚举

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3β,20α-羟基甾体脱氢酶的动力学性质

生物化学杂志 1991年第2期7卷 177-182页

作者：陈清轩 colin D.Nancarrow Frederick Sweet 中国科学院发育生物学研究所 CSIRO Division of Animal Production Prospect NSW 2148 Australia Depart.of OB/CYN Washington University School of Medicine St. LouisMO 63110 U.S.A.

3β,20α-羟基甾体脱氢酶(3β,20α-Hydroxysteroid dehydrogenase,3β,20α-HSD)是从胎羊血中分离得到的。分子量为35kD。该酶以NADPH为辅酶,有两种底物。以孕酮为底物时,Km=30.8μmol/L,Vmax=0.7nmol min^(-1)(nmol enzyme)^(-1);以5α-二氢睾酮(5α-Dihydrotestosterone,5α-DHt)为底物时,Km=74μmol/L,Vmax=1.3nmol min^(-1)(nmol enzyme)^(-1)。5α-DHt竞争性抑制20α-还原活性,Ki=102μmol/L。16α-溴代乙酰氧基(16α-Bromo acetoxyprogesterone,16α-BAP)是3β,20α-HSD不可逆竞争性抑制剂,t_(1/2)=75min。对3β和20α还原活性的抑制常数Ki分别为23μmol/L和58μmol/L。

关键词：羟甾体脱氢酶动力学

Micro-ESR:微型电子自旋共振光谱仪

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生命科学仪器 2011年第3期9卷 23-26页

作者： Christopher J.WHItE colin t.ELLIOtt James R.WHItE Active Spectrum Inc.

电子自旋共振光谱(ESR),又称顺磁共振光谱(EPR),是直接检测自由基、引起石油氧化降解含有未成对电子的活性分子以及食物的唯一方法,也在生物化学研究疾病方面起着一定的作用。本文所描述的微型、高灵敏度的ESR光谱仪通过显著降低光谱技... 详细信息

电子自旋共振光谱(ESR),又称顺磁共振光谱(EPR),是直接检测自由基、引起石油氧化降解含有未成对电子的活性分子以及食物的唯一方法,也在生物化学研究疾病方面起着一定的作用。本文所描述的微型、高灵敏度的ESR光谱仪通过显著降低光谱技术的成本,能够为工业和科学研究者扩大ESR光谱学的实用范围。

关键词：电子自旋共振光谱光谱仪微型科学研究者顺磁共振直接检测活性分子氧化降解

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不要有错必罚

新东方英语（中英文版） 2006年第9期 74-75页

作者： colin Powell 程悦

科林·鲍威尔(colinPowell)是美国历史上第一位黑人国务卿,也是美国政坛地位最高的一名黑人。在以态度强硬、作风凌厉著称的小布什政府中,鲍威尔是核心人物中惟一的一位温和派人物,通常被人们视为比较典型的“鸽派”人物。他待人热情随... 详细信息

科林·鲍威尔(colinPowell)是美国历史上第一位黑人国务卿,也是美国政坛地位最高的一名黑人。在以态度强硬、作风凌厉著称的小布什政府中,鲍威尔是核心人物中惟一的一位温和派人物,通常被人们视为比较典型的“鸽派”人物。他待人热情随和,具有很强的亲和力。鲍威尔年轻时曾经入伍,在完成了步兵军官基础训练后,被派至德国,其间发生的一件事,对他以后的领导风格产生了很大的影响……

关键词：鲍威尔步兵米勒手枪黑人小布什政府美国历史基础训练领导风格德国

Finding Areas at Risk from Floods in a Downpour Using the Lidar-Based Elevation Model

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Journal of Civil Engineering and Architecture 2021年第1期15卷 1-16页

作者： Sultana Nasrin Baby colin Arrowsmith Gang-Jun Liu David Mitchell Nadhir Al-Ansari Nahala Abbas School of Mathematical and Geospatial Sciences Royal Melbourne Institute of TechnologyRMIT UniversityGPO Box 2476Melbourne 3001Australia Lulea University of technology Lulea 97187Sweden School of Engineering&technology Central Queensland UniversityMelbourneVIC 3000Australia

Climate change can impact coastal areas in different ways,including flooding,storm surges,and beach *** these,flooding has a major impact on the operation of coastal drainage *** paper develops a new flood screening model using a LiDAR based digital elevation model(DEM)to improve the implementation of Victorian’s coastal flooding risk assessment and *** elevation models are directed towards protection from cloudbursts and applied to rising sea *** aim is to simulate water flow on the ground and in streams,and the resulting accumulation of water in depressions of the blue spot using *** to the presence of pipes,watercourses,bridges and channels it was required that the DEM data to be *** reservoirs of rain will prevent seawater from flowing across the stream channel into *** rain drain will be open during normal sea levels to allow rain water in the river to move and flow in to the ***,geographic information system(GIS)assists with spatial data management,but lacks modelling capability for complex hydrology problems and cannot be relied upon by decision-makers in this *** improvements are therefore required to improve the processing or analytical capabilities of GIS in *** research shows how the spatial data can be primarily processed by GIS adopting the spatial analysis routines associated with *** objective of this paper is to outline the importance of GIS technology for coastal flood *** a definition of the coastal flood,and,short description of its peculiarities and the urgency of its management,this paper describes the use of GIS technology in coastal flood management,its advantages and the consideration for *** is followed by the information and LiDAR data required for coastal flood management and the application area in coastal flood *** paper method is presented to conduct a first high-resolution DEM screening t

关键词： LiDAR flood-risk model builder blue spot model ESRI DEM

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错误·成长

疯狂英语（阅读版） 2004年第12期 38-39页

作者： colin Powell 朱沛杰

我年轻的时候曾是一名步兵军官。在一次任务中,我被派到了第48步兵团、驻地离德国的法兰克福不远。当时我们有一件战利品,那就是口径为280毫米的巨型原子炮。每架大炮都由几个排的步兵守护,用卡车载着在树林中穿行。

关键词：德国原子炮步兵法兰克福战利品米勒手枪弹膛军官卡车

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1型糖尿病疫苗研究(英文)

1型糖尿病疫苗研究(英文)

2006（第三届）江浙沪儿科学术会议暨浙江省儿科学术年会

作者： colin Dayan 英国布利斯托大学皇家医院内分泌科

type 1 diabetes is an autoimmune condition in which self-reactive t cells cause β-cell destruction． Studies in recent years have shown that people at risk for type 1 diabetes cna be identified 5 or more years before they develop the disease by the presence of serum islet-specific *** provides an opportunity for intervention against the autoimmune process that might prevent at risk individuals developing diabetes at *** treatments such as cyclosporin are known to stop the autoimmune process,but are too toxic for lifelong therapy．

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来源： cnki会议评论

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不要逢错便罚

英语教师 2007年第1期7卷 46-47页

作者： colin Powell 咏希

作为一名年轻的步兵军官,在一次执行任务中,我被分派到了位于德国法兰克福附近的第48步兵团。那时我们最有力的武器要属口径为280毫米的大型原子炮。每个大炮都要由几个排的步兵来守护,还要用卡车拉着在森林周围转来转去,以防苏联军队... 详细信息

作为一名年轻的步兵军官,在一次执行任务中,我被分派到了位于德国法兰克福附近的第48步兵团。那时我们最有力的武器要属口径为280毫米的大型原子炮。每个大炮都要由几个排的步兵来守护,还要用卡车拉着在森林周围转来转去,以防苏联军队猜出大炮的位置。

关键词：步兵原子炮法兰克福大炮军队武器任务米勒兵团口径