检索结果-南通市图书馆

Antibiotic Resistance Profile of Salmonella Strains Isolated at the National Clinical Biology and Public Health Laboratory in Bangui, Central African Republic

引用

Health 2024年第2期16卷 160-171页

作者： Marceline Djeintote Ernest Lango-Yaya Zéphirin-Dalengat Vogbia clotaire don.tien.rafaï Augustin Balekouzou Henri Saint-Claver Djemer Wilfried Sylvain Nambei Boniface Koffi Gérard Gresenguet National Blood Transfusion Center Bangui Central African Republic Faculty of Health Sciences University of Bangui Bangui Central African Republic National Laboratory of Clinical Biology and Public Health Bangui Central African Republic National Reference Center for Sexually Transmitted Infections and Anti-Retroviral Therapy Bangui Central African Republic National Center for the Fight against AIDS Bangui Central African Republic

In Africa, each year, there are estimated to be more than 91 million cases of salmonellosis and 137,000 cases of death. The problem of antibiotic resistance in Salmonella strains is a threat to public health. The objective of this study is to evaluate the antibiotic resistance profile of Salmonella strains isolated in biological products analyzed at the National Laboratory of Clinical Biology and Public Health (NLCBPH) in Bangui. This is, therefore, a cross-sectional study with a descriptive aim, running from January to December 2022. It focused on the strains of Salmonella isolated and identified in stools, urines, and blood samples. For each strain of Salmonella isolated, an antibiogram was carried out following the recommendations of the French Society of Microbiology (CASFM, 2022). A total of 93 strains of Salmonella have been recorded. The age group 0 - 9 years was 29% and that of >50 years was 11%. The median age of patients was 30 years with a minimum of 1 and a maximum of 78 years. The female gender was more represented at 52.69% than the male gender at 47.31%, i.e. a sex ratio of 0.89 (M/F). Salmonella strains were much more isolated in stools at 62% followed by urines at 29% and blood at 6%. Salmonella arizonae strains were more represented with 52%. Salmonella strains have a resistance rate to Tetracycline of 62.37% followed by Penicillins of 50%. The rate of multi-antibiotic resistance of the Salmonella strains isolated represented 48.38%. Salmonella spp. strains were multi-resistant at 58.69% followed by Salmonella arizonae strains at 47.91%. There is a significant association between the different families of antibiotics and Salmonella strains (p < 0.05). According to the results obtained, Penicillins, Phenicoles, and Cyclins had a high rate of resistance on Salmonella strains. No strain-producing Broad Spectrum Beta-lactamase has been isolated. Salmonella strains represent a zoonotic health danger, constitute a public health problem and remain a curre

关键词： Salmonella Resistance Antibiotics Central African Republic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antibiotic Resistance Profile of Serotypes of Streptococcus pneumoniae Strains in Bangui, from 2017 to 2022: Case of Serotype 1

引用

Open Journal of Medical Microbiology 2024年第2期14卷 131-145页

作者： Zéphirin Dalengat Vogbia Ernest Lango Yaya Marceline Djeintote Stéphanie Judith N’Yetobouko Jean de Dieu Longo clotaire don.tien.rafaï Christian Diamant Mossoro-Kpindet Gérard Gresenguet Epidemiology and Research Service National Reference Center for Sexually Transmitted Infections and Anti-Retroviral Therapy Ministry of Health Bangui Central African Republic Faculty of Health Sciences Doctoral School of Human and Veterinary Health Sciences University of Bangui Bangui Central African Republic National Laboratory of Clinical Biology and Public Health Bangui Central African Republic National Blood Transfusion Center Bangui Central African Republic Public Department Ministry of Health Bangui Central African Republic

Goals: The aim of this study was to determine the antibiotic resistance profile of serotypes of Streptococcus pneumoniae strains circulating in Bangui. Methodology: A prospective and analytical analysis was carried out at the National Laboratory of Clinical Biology and Public Health from 2017 to 2022. The strains came from our study on the contribution to the study of antibiotic sensitivity of Streptococcus pneumoniae strains. The multiplex PCR test was used for its cost-effectiveness in terms of amplifiers which can be purified in order to be sequenced. It also makes it possible to detect several germs as well as their serotypes. For a PCR reaction, several elements are involved in the reaction medium or Master Mix. These are the desoxyribonucleotides (dNTPs), the magnesium ions (MgCl2) and the primers. A set of 14 primers divided into 3 classes were used. Class 1 primers served as an internal control by targeting the cpsA gene. It is a highly conserved gene found in capsular loci characterized to date. The primers of the second class were used to target specific serotypes by specific reactions (out of six possibilities). The group reaction was carried out using the primers of the third class in order to carry out an initial screening of the samples and to classify the pneumococcal isolates. Related serotypes were grouped based on the amplification of common genes. Using the technique of electrophoresis on agarose gel and an ultraviolet radiation device, the migration bands are then visualized and analyzed. The data collected had been entered into Excel 2010 and analyzed with Epi info 7. The exact Fischer chi2 test at the 5% threshold, the relative risk and its 95% confidence interval were used to compare the proportions and determine the associations. Results: 187 antibiotic-resistant strains of Streptococcus pneumoniae were collected. The average frequency of serotypes 1, 9A, 4 and untypeable identified were 43.59%, 18.18%, 18.27% and 39.57% respectively. The fre

关键词： Streptococcus pneumonia Serotype Antibiotic Resistance Bangui

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Contribution of Genomic Surveillance in the Detection and Monitoring of SARS-CoV-2 Variants during the 6 Pandemic Waves in the Central African Republic from 2020 to 2023

引用

Journal of Tuberculosis Research 2024年第3期12卷 151-164页

作者： clotaire don.tien.rafaï Ernest Lango-Yaya Marie Roseline Darnicka Belizaire Ouoko Fa-Ti-Gbia Maurel Annicet Adonis Marcel Mbeko Simaleko Jean-Baptiste Roungou Oscar Senzongo Karl G. Njuwa Fai Boniface Koffi National Laboratory of Clinical Biology and Public Health of Bangui Bangui Republic Central African University of Bangui Bangui Central African Republic World Health Organization Geneva Switzerland Ministry of Health and Population Bangui Central African Republic Africa CDC Addis-Abeba Ethiopia Homegrown Solutions for Health Yaounde Cameroon

Objective: The COVID-19 pandemic has highlighted the need to strengthen diagnosis and genomic surveillance capacities. In 2021, Central African managed five waves of COVID-19 by integrating genomic surveillance into their health monitoring system. This study sought to report surveillance data from the National Laboratory of Clinical Biology and Public Health and describe the circulation of SARS-CoV-2 variants. Materials and Methods: This retrospective, descriptive observational study spans three years, from April 2020 to November 2023. It was conducted on a population of consenting volunteers from across the Central African Republic, who were tested using RT-PCR on nasopharyngeal samples. Data with sufficient information were obtained from the National Laboratory of Clinical Biology and Public Health (LNBCSP) databases. Sequencing was largely carried out at the National Institute of Biomedical Research (INRB) in Kinshasa until May 2023, and subsequently at the LNBCSP. Results and Discussion: Out of 97,864 RT-PCR tests performed, 9,764 were positive, resulting in a prevalence of 9.98%. The average age of the patients was 39.97 years ± 13.76, and the male-to-female sex ratio was 2.12. RT-PCR test positivity was significantly associated with age (p = 0.001), sex (p = 0.013) and clinical manifestations. Ten variants circulated during the five recorded waves, with Omicron (B.1.1.529), Delta (B.1.617.2) variants being predominant. Notably, the B.1.620 and B.640 variants were prominent during the second wave. Conclusion: This retrospective study provides key insights into the COVID-19 pandemic in the CAR. It identifies risk factors and details the circulation of various SARS-CoV-2 variants. Enhancing national genomic surveillance capacities would enable the country to better respond to future pandemic challenges.

关键词： SARS-CoV-2 Variants Central African Republic RT-PCR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mass Screening Using Antigenic and Molecular Diagnostic Tests of COVID-19 in Bangui at the Beginning of the Second Wave in July 2021

引用

Journal of Tuberculosis Research 2024年第1期12卷 1-12页

作者： clotaire don.tien.rafaï Marie-Roseline Darnycka Belizaire Pierre Somse Wilfrid Sylvain Nambei Ernest Lango-Yaya Oscar Senzongo Luc Salva Hereidebona Ulrich Jeffrey Kotemossoua Rabbi Mermoz Senekian Simon Pounguinza Christian-Diamant Mossoro-Kpinde Alain Le Faou Jean De Dieu Longo François-Xavier Mbopi-Keou Gérard Grésenguet Boniface Koffi National Laboratory of Clinical Biology and Public Health Bangui Central African Republic Faculty of Health Sciences University of Bangui Bangui Central African Republic World Health Organization Bangui Central African Republic Ministry of Health and Population Bangui Central African Republic Faculty of Medicine Maieutic and Health Sciences University of Lorraine Pole Brabois Santé Nancy France Faculty of Medicine and Biomedical Sciences University of Yaounde I Yaounde Cameroun

Objective: COVID-19 surveillance was established as early as March 2020 in the Central African Republic (CAR), after the WHO statement relating to the identification of several cases outside China. However, given the non-performing molecular biology technical platform in many developing countries in sub-Saharan Africa, the second wave promised to be surprising and formidable. In this context, a mass survey was launched in Bangui to determine the prevalence of COVID-19. Patients and Methods: From March 18 to April 2, 2021, a mass screening campaign took place in tourist places, companies and the main hospital infrastructures. Nasopharyngeal swab samples were collected from participants with and without symptoms of Influenza-like illness (ILI) and stored in VTM tubes. The Ag (COVID-19) and RT-PCR tests were carried out in Bangui at the LNBCSP. The sequencing of RT-PCR SARS-CoV-2 positives was carried out at the INRB. Results: We included 1480 participants of whom 33 (2.23%) were SARS-COV-2 positive, of whom 24 were male and 9 female. This sex difference was statistically significant (p = 0.012) as the sex ratio M/F was 1.09. Sampling sites located in the 1st arrondissement were the most prolific (p = 0.006) and were sequenced. In addition to the analysis of the 33 samples from the predefined sites under study, 17 control sequences from the provinces generated during the same period are added. We detected 2 Variants Of Concern (VOC) including the predominant B.1.620 (43.86%) followed by B.1.1.7 or Alpha (5.10%). Conclusion: The study showed the importance of surveillance and the availability of means of diagnosis of COVID-19. The identified risk factors were sex and sampling site. This study has shown the importance of setting up sentinel sites for COVID-19 surveillance in all regions of the country and the appropriate use of the anti-COVID-19 vaccine.

关键词： COVID-19 Variants Central African Republic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Contribution to the Study of Fungal Strains Contaminating Peanut Pastes in Bangui (Central African Republic)

引用

Journal of Biomedical Science and Engineering 2024年第5期17卷 97-106页

作者： Romaric Lebon Bondom Ernest Lango-Yaya Mireille Morissi Denissio clotaire don.tien.rafai Alban Thibaut Moulo-Oli Zéphirin Vogbia Dalenga Marceline Djeintote Mossoro-Kpindé Boniface Koffi Laboratory of Biomedical Sciences (LASBM) Faculty of Health Sciences University of Bangui Bangui Central African Republic National Laboratory of Clinical Biology and Public Health Ministry of Health and Public Population Bangui Central African Republic

Introduction: Peanut pastes are food products resulting from artisanal or industrial processing, used in cooking in Africa in general and in Central African Republic in particular. These peanut pastes are often contaminated by molds and filamentous fungi involved in the degradation of hygienic and organoleptic or even toxicological quality. This study aims to determine the epidemiological profile of molds contaminating peanut pastes sold on the Central African market. Methodology: This was a cross-sectional study carried out from June to September 2023. Samples of peanut pastes sold on Central African market were taken and analyzed at the National Laboratory of Clinical Biology and Public Health using the conventional microbiology method according to ISO 7954 standards. The data obtained were collected in the ODK 2023.3.1 application and analyzed with the Epi Info 7 software. A multivariate analysis by logistic regression, Ficher’s exact test, and chi2 at the 5% threshold (p Penicillium sp.;11.25% of Mucor sp.;10.63% of Aspergillus terrei;3.13% of Aspergillus niger;1.25% of Aspergillus medullans;28.13% of Aspergillus flavus;2.50% of Aspergillus fumigatus. Peanut pastes stored beyond three days were more contaminated (94.19%). Conclusion: The results of this study made it possible to highlight strains of mold that impact the hygienic and organoleptic quality of peanut pastes sold at the Central African market. Most of the isolated strains were the Aspergillus flavus species which is recognized by its toxigenic effects. This species is much more incriminated in the contamination of foodstuffs with the production of the toxin which causes underlying pulmonary pathologies in humans.

关键词： Peanut Pastes Fungal Contamination Central African Republic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Impact of a Mass Campaign on the Evolution of the Fourth Wave of the Covid-19 Pandemic in the Central African Republic

引用

Journal of Tuberculosis Research 2024年第3期12卷 142-150页

作者： clotaire don.tien.rafaï Pierre Somse Wilfrid Sylvain Nambei Ernest Lango-Yaya Marie-Roseline Darnycka Belizaire Ulrich Vickos Narcisse Patrice Komas Oscar Senzongo Luc Salva Heredeibona Ulrich Jeffrey Kotemossoua Rabbi Mermoz Senekian Simon Pounguinza Jephté Estimé Kaleb Christian-Diamant Mossoro-Kpinde Alain Le Faou Jean De Dieu Longo Norbert Richard Ngbale Abdoulaye Sepou François-Xavier Mbopi-Keou Gérard Grésenguet Boniface Koffi National Laboratory of Clinical Biology and Public Health Bangui Central African Republic Faculty of Health Sciences University of Bangui Bangui Central African Republic Ministry of Health and Population Bangui Central African Republic World Health Organization Bangui Central African Republic Pasteur Institute of Bangui Bangui Central African Republic Faculty of Medicine Midwifery and Health Professions University of Lorraine Nancy France Faculty of Medicine and Biomedical Sciences University of Yaounde I Yaounde Cameroun Community University Hospital Center of Bangui Bangui Central African Republic

Objective: In the context of increasing cases despite vaccination campaigns, a survey was conducted in the Bangui population from January 17 to 26, 2022, to evaluate the strains of Severe Acute Respiratory Infection Coronavirus 2 (SARS-Cov-2) circulating in a healthy population. Materials and methods: This study was conducted by taking nasopharyngeal samples from randomly selected volunteers. Antigen detection was performed systematically, and RT-PCR was done on the positive samples. Results and discussion: We collected 2,554 samples. Thirty were found RT-PCR positive (1.2%) and sent for viral genome sequencing. Twenty-eight SARS-Cov-2 strains belonged to the Omicron type, and only 2 to the Delta type. Conclusion: Thus, infections were uncommon in the tested population, but the presence of Omicron and Delta types raises concerns that vaccination may not be effective in fighting the virus, and newly designed vaccines should be implemented to better protect the population at risk of infection and reinfection by these variants.

关键词： COVID-19 SARS-CoV-2 Variants

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antibiotic Resistance Profile of Serotypes of Streptococcus pneumoniae Strains in Bangui,from 2017 to 2022: Case of Serotype 1

引用

Open Journal of Medical Microbiology（医学微生物学） 2024年第2期 131页

来源：博看期刊评论

在线全文

博看期刊

学校读者我要写书评

暂无评论

Contribution to the Study of Fungal Strains Contaminating Peanut Pastes in Bangui (Central African Republic)

引用

Journal of Biomedical Science and Engineering（生物医学工程） 2024年第5期 97页

作者： Romaric Lebon Bondom Ernest Lango-Yaya Mireille Morissi Denissio clotaire don.tien.rafai Alban Thibaut Moulo-Oli Zéphirin Vogbia Dalenga Marceline Djeintote Mossoro-Kpindé Boniface Koffi

来源：博看期刊评论

在线全文

博看期刊

学校读者我要写书评

暂无评论

MiR-26b是前列腺癌中的抑癌微RNA(英文)

引用

生物化学与生物物理进展 2017年第6期44卷 504-514页

作者： Daguia Zambe John clotaire 王蕴非黄伟伟赵飞张斌雷鸣西北农林科技大学生命科学学院杨凌712100 Faculty of Science University of Bangui Central Africa 中国科学院生物物理研究所北京100101

微RNAs(又称miRNAs或miRs)是一类长度为19~24个核苷酸的单链非编码RNA分子.miRNA通过与其靶向的mRNA分子序列特异性互补配对,调节mRNA表达水平,抑制转录后的蛋白质翻译.miRNA在肿瘤中既可作为致癌因子也可作为抑癌因子.本研究前期已报道... 详细信息

微RNAs(又称miRNAs或miRs)是一类长度为19~24个核苷酸的单链非编码RNA分子.miRNA通过与其靶向的mRNA分子序列特异性互补配对,调节mRNA表达水平,抑制转录后的蛋白质翻译.miRNA在肿瘤中既可作为致癌因子也可作为抑癌因子.本研究前期已报道miR-26b在前列腺癌细胞系中低表达,并且抑制细胞自噬.本研究进一步全面揭示miR-26b对前列腺肿瘤细胞的作用.我们发现过表达miR-26b能够在体外抑制前列腺癌细胞的增殖和侵袭,并抑制裸鼠体内原位异种前列腺肿瘤的生长.为了探究miR-26b对前列腺癌细胞增殖和侵袭的潜在调控机制,我们进行了表达谱芯片鉴定miR-26b调控基因.表达谱芯片分析表明,在前列腺癌细胞系PC-3中过表达miR-26b后,显著上调的基因57个,显著下调的基因55个(变化倍数均大于2,且P值小于0.05).差异基因的功能多与细胞增殖、凋亡调控、蛋白质磷酸化和泛素化修饰调控过程相关,并且富集在多种信号通路中,例如TNF和TGF-β信号通路.在这些筛选出的基因中,CEACAM6表达水平下调2.17倍;序列分析及实验验证表明,CEACAM6的3′UTR区存在miR-26b的互补序列,是miR-26b的直接靶标.本研究证明了miR-26b能够靶向结合抑制CEACAM6的表达,从而抑制前列腺癌细胞在体外和体内的细胞增殖和侵袭活性,miR-26b是前列腺癌中的抑癌microRNA.

关键词： miR-26b 前列腺癌细胞细胞增殖和侵袭表达谱芯片 CEACAM6

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Contribution to the Study of Antibiotic Sensitivity of Streptococcus pneumoniae Strains in Spinal Cerebral Fluids in Bangui from 2017 to 2022

引用

Journal of Biomedical Science and Engineering 2023年第7期16卷 95-106页

作者： Zéphirin-Dalengat Vogbia Ernest Lango Yaya Marie Colette-Nganda Banguet Jean de Dieu Longo clotaire -rafaï Maurel Anicet Adonis Ouoko Fa-Tigbia Christian Diamant Mossoro Gérard Gresenguet Ministry of Health Epidemiology and National Reference Center for Sexually Transmitted Infections and Anti-Retroviral Therapy Research Service Bangui Central African Republic Faculty of Health Sciences University of Bangui Bangui Central African Republic Ministry of Health National Laboratory of Clinical Biology and Public Health Bangui Central African Republic Emergency Department at the Pediatric University Complex Bangui Central African Republic Ministry of Health Training Project Manager and Head of Public Department Bangui Central African Republic

A prospective and analytical study was carried out from January 2017 to Decem-ber 2022, at the National Laboratory of Clinical Biology and Public Health (LNBCSP) in Bangui. 352 samples of cerebrospinal fluid (CSF) were confirmed out of 2065, coming from the four hospitals in Bangui. This study aimed to evalu-ate the evolution of antibiotic sensitivity to strains of Streptococcus pneumoniae. CSF had been collected from patients who presented with meningeal syndromes. Based on the leukocyte count (White blood cells ≥ 5 mm3), an aliquot was trans-ferred to trans-isolate medium and sent to the LNBCSP for confirmatory testing, culture and determination of antibiotic sensitivity. The antibiotic sensitivity of Streptococcus pneumoniae strains was tested according to the methods recom-mended by the Antibiogram Committee of the French Society of Microbiology. The data collected was entered into Excel 2010 to be analyzed with Epi Info 7.2. Ficher’s exact test, chi2 at the 5% threshold (p < 0.05) was used to compare pro-portions and analyze associations between variables. The average sensitivity rate to β-lactams was 74.43%. The sensitivity rate of Fluoroquinolones was 54.54%. That of levofloxacin was 87%. The average rate of sensitivity to β-lactams for the age group under 5 years old was 79.25%. That of fluoroquinolones was 52.59%. Levofloxacin had 90.37%. The average sensitivity rate to β-lactams for the age group over 5 years old was 76.03%. Fluoroquinolones had 45.16%. Levofloxacin had 69.58%. The average sensitivity rate to β-lactams for males was 76.68%. Fluoroquinolones had 54.26%. That of levofloxacin was 83.40%. The sensitivity rate to β-lactams for females was 74.41%. That of fluoroquinolones was 51.16%. Levofloxacin had 67.44%. Cyclins had 28.68%. The study noted an association between age and sensitivity (p < 0.05;CI [1.05-2.57]). Strains of Streptococcus pneumoniae were always detected in the CSF. The average rate of sensitivity to macrolides was 36.93%;aminoglyc

关键词： Streptococcus pneumoniae Sensitivity Antibiotics Central African Republic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：