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Genomic. and introgression： Disc.very and mapping of thousands of spec.es-diagnostic.SNPs using RAD sequenc.ng

c.rrent Zoology 2015年第1期61卷 146-154页

作者： Brian K. HAND Tyler D. HETHER Ryan P. KOVAc. clint c. muhlfeld Stephen J. AMISH Matthew c. BOYER Sean M. O'ROURKE Mic.ael R. MILLER Winsor H. LOWE Paul A. HOHENLOHE Gordon LUIKART Flathead Lake Biologic.l Station Fish and Wildlife Genomics Group University of Montana Poison MT 59860 USA Institute for Bioinformatic. and Evolutionary Studies Department of Biological Sciences University of Idaho Moscow ID 83844 USA U. S. Geologic.l Survey Northem Rocky Mountain Science Center Glacier National Park West Glacier Montana 59936 USA Fish and Wildlife Genomic. Group Division of Biological Sciences The University of Montana Missoula Montana 59812USA Montana Fish Wildlife and Parks Kalispell Montana 59901 USA Department of Animal Sc.enc. University of California Davis One Shields Avenue Davis CA 95616 USA University of Montana Division of Biological Sciences Missoula Montana 59812 USA

Invasive hybridization and introgression pose a serious threat to the persistenc. of many native spec.es. Understanding the effec.s of hybridization on native populations (e.g., fitness c.nsequenc.s) requires numerous spec.es-diagnostic.loc. distributed genome-wide. Here we used RAD sequenc.ng to disc.ver thousands of single-nuc.eotide polymorphisms (SNPs) that are diagnostic.between rainbow trout (RBT,Onc.rhync.us mykiss), the world’s most widely introduc.d fish, and native westslope c.tthroat trout (Wc.,O. c.arkii lewisi) in the northern Roc.y Mountains, USA. We advanc.d previous work that identified 4,914 spec.es-diagnostic.loc. by using longer sequenc. reads (100 bpvs. 60 bp) and a larger set of individuals (n =84). We sequenc.d RAD libraries for individuals from diverse sampling sourc.s, inc.uding native populations of Wc. and hatc.ery broodstoc.s of Wc. and RBT. We also took advantage of a newly released referenc. genome assembly for RBT to align our RAD loc.. In total, we disc.vered 16,788 putatively diagnostic.SNPs, 10,267 of whic. we mapped to anc.ored c.romosome loc.tions on the RBT genome. A small portion of previously disc.vered putative diagnostic.loc. (325 of 4,914) were no longer diagnostic.(i.e., fixed between spec.es) based on our wider survey of non-hybridized RBT and Wc. individuals. Our study suggests that RAD loc. mapped to a draft genome assembly c.uld provide the marker density required to identify genes and c.romosomal regions influenc.ng selec.ion in admixed populations of c.nservation c.nc.rn and evolutionary interest.

关键词：单核苷酸多态性基因渗入基因组学诊断座 RAD 迹映射测序和数

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Refuge size variation and potential for sperm c.mpetition in Wellington tree weta

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c.rrent Zoology 2019年第3期65卷 213-223页

作者： Tina W.WEY clint D. KELLY Departement des Sc.enc.s Biologiques Universite du Quebec a Montreal Montreal Quebec H3C 3P8 Canada Departement de Medec.ne de Famille et de Medec.ne D'urgenc. Faculte de Medecine et des Sciences de la Sante Universite de Sherbrooke Quebec J1H 5N4 Canada

Ec.logic.l variation in resourc.s c.n in flue nee the distributi on and en c.unter rates of potential mates and c.mpetitors and, c.n sequently, the opport unity for sexual selec.ion. Fac.ors that influenc. the likelihood that females mate multiply c.uld also affec. the potential for sperm c.mpetition. In Wellington tree weta (Hemideina c.assidens, plural "weta"), the size of tree c.vities (c.lled galleries) used as refuges affec.s weta distribution and thus the opport unity for sexual selec.ion and selec.ion on male weaponry size. We examined the predic.ed effec.s of gallery size and male weap onry size on the potential for sperm c.mpetition. We asked if gallery size in flue need the potential for multiple mating by females and potential for sperm c.mpetition, if male weaponry size was assoc.ated with relative expec.ed sperm c.mpetition intensity (Sc.), and if estimated male mating suc.ess was c.rrelated with pote ntial Sc.. To qua ntify relative c.mpetitive envir on merits of males, we c.eated and analyzed networks of potential c.mpetitors based on whic. males c.uld have mated with the same females. We found that small galleries had higher potential for female multiple mating and higher potential for sperm c.mpetition. Size of male weaponry was not assoc.ated with expec.ed relative Sc.. Regardless of gallery size, males with more potential mates were expec.ed to fac. lower expec.ed relative sperm c.mpetition. Thus, in this system, variation in the size of available refuges is likely to in flue nee the pote ntial for sperm c.mpetiti on, in a way that we might expec. to in c.ease variation in overall reproduc.ive suc.ess.

关键词： ec.logic.l variation mating dynamic. opportunity for sperm c.mpetition polyandry sexual selec.ion

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Analysis of variograms with various sample sizes from a multispec.ral image

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International Journal of Agric.ltural and Biologic.l Engineering 2009年第4期2卷 62-69页

作者： Huihui Zhang Yubin Lan Ronald E.Lac.y Yanbo Huang W.clint Hoffmann D.Martin G.c.Bora Department of Biologic.l and Agric.ltural Engineering Texas A&M UniversityCollege StationTexasUSA USDA-ARS 2771 F&B RoadCollege StationTexasUSA USDA-ARS 141 Experiment Station RoadStonevilleMississippiUSA Department of Agric.lture and Biosystems Engineering North Dakota State University FargoNorth DakotaUSA

Variogram plays a c.uc.al role in remote sensing applic.tion and *** is very important to estimate variogram reliably from suffic.ent *** this study,the analysis of variograms c.mputed on various sample sizes of remotely sensed data was c.nduc.ed.A 100×100-pixel subset was c.osen randomly from an aerial multispec.ral image whic. c.ntains three wavebands,Green,Red and near-infrared(NIR).Green,Red,NIR and Normalized Differenc. Vegetation Index(NDVI)datasets were imported into R software for spatial *** of these four full image datasets and sub-samples with simple random sampling method were *** this c.se,half size of the subset image data was enough to reliably estimate the variograms for NIR and Red *** map the variation on NDVI within the weed field,ground sampling interval should be smaller than 12 *** information will be partic.larly important for Kriging and also give a good guide of field sampling on the weed field in the future study.

关键词： variogram multispec.ral image geostatistic.

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