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Drosophila X virus-like particles as delivery carriers for improved oral insecticidal efficacy of scorpion Androctonus australis peptide against the invasive fruit fly,Drosophila suzukii

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Insect Science 2024年第3期31卷 847-858页

作者： Qi Xue Luc Swevers clauvis nji tizi taning Faculty of Bioscience Engineering Department of Plants and CropsGhent UniversityGhentBelgium Insect Molecular Genetics and Biotechnology Institute of Biosciences and ApplicationsNational Centre for Scientific Research"Demokritos"AthensGreece

Insect-specific neurotoxic peptides derived from the venoms of scorpions and spiders can cause acute paralysis and death when injected into insects,offering a promising insecticidal component for insect pest ***,effective delivery systems are required to help neurotoxic peptides pass through the gut barrier into the hemolymph,where they can ***,we investigated the potential of a novel nanocarrier,Drosophila X virus-like particle(DXV-VLP),for delivering a neurotoxin from the scorpion Androctonus australis Hector(AaIT)against the invasive pest fruit fly,Drosophila *** results show that the fusion proteins of DXV polyproteins with AalT peptide at their Ctermini could be sufficiently produced in Lepidoptera Hi5 cells in a soluble form using the recombinant baculovirus expression system,and could self-assemble into VLPs with similar particle morphology and size to authentic DXV *** addition,the AalT peptides displayed on DXV-VLPs retained their toxicity,as demonstrated in injection bioassays that resulted in severe mortality(72%)in adults after 72 *** fed to adults,mild mortality was observed in the group treated with DXV-AalT(38%),while no mortality occurred in the group treated with AaIT peptide,thus indicating the significant role of DXV-VLPs in delivering AalT ***,this proof-of-concept study demonstrates for the first time that VLPs can be exploited to enhance oral delivery of insect-specific neurotoxic peptides in the context of pest ***,it provides insights for further improvements and potentially the development of neurotoxin-based bioinsecticides and/or transgenic crops for insect pest control.

关键词： AalT Drosophila suzuki nanocarrier neurotoxic peptides pest control virus-like particle(VLP)

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Myosuppressin influences fecundity in the Colorado potato beetle,Leptinotarsa decemlineata

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Insect Science 2021年第4期28卷 1191-1201页

作者： Shun-Hua Gui clauvis nji tizi taning Guy Smagghe Laboratory of Agrozoology Department of Plants and CorpsGhent UniversityGhentBelgium

Insect neuropeptides regulate various physiological processes,such as reproduction,feeding,growth and development,and have been considered as viable targets in the development of alternative strategies for pest *** these neuropeptides is myosuppressin(MS),a very conserved neuropeptide that has been reported to regulate cardiac and skeletal muscle contractility,feeding and pupal diapause in *** this study,we investigated the involvement of MS in fecundity in a notorious defoliator of potato and other solanaceous plants,the Colorado potato beetle(CPB),Leptinotarsa *** identified an MS-precursor-encoding transcript in the *** transcriptomic database and then evaluated its transcript levels in various CPB *** transcript levels were found to be highest in the central nervous system,gut and muscle of CPB males and *** investigate the role of MS in fecundity,MS was silenced in adult CPBs through RNA interference(RNAi).This resulted in a significant reduction in oviposition(over 80%)and oocyte size(69%)in the treated beetles compared to the ***,the reduction in oviposition in treated females was confirmed to be dependent on MS knockdown and independent of male ***,MS-knockdown in females resulted in decreased levels of ecdysteroid hormone titers and the transcript levels of its ***,the injection of 20-hydroxyecdysone into females following MS knockdown could rescue ovary ***,this study highlights the important role played by MS in regulating fecundity in CPB.

关键词： ecdysteroid fecundity Leptinotarsa decemlineata myosuppressin neuropeptide

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