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Characterization of *** chromosome addition lines DA2H and MA5H

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Journal of Genetics and Genomics 2008年第11期35卷 673-678页

作者： Fang Kong Haiyan Wang Aizhong Cao Bi Qin jianhui ji Suling Wang Xiu-e Wang State Key Laboratory of Crop Genetics and Germplasm enhancement Cytogenetics Institute Nanjing Agricultural University Nanjing 210095 China

in order to transfer useful genes of Hordeum californicum into common wheat （Triticum aestivum L.）, the T. aestivum c.v. Chinese Spring （CS）-H. californicum amphiploid was crossed to CS, and its backcrossing and self-fertilized progenies were analyzed by morpho- logical observation, cytological, biochemical and molecular marker techniques. Alien addition lines with two H. californicum chromo- somes were identified and their genetic constitution was characterized. STS-PCR analysis using chromosome 2B specific markers indi- cated that chromosome H3 of H. californicum belongs to homoeologous group 2, and was thus designated 2H. SDS-PAGe showed that chromosome H2 of H. californicum belongs to homoeologous group 5, and was designated 5H. The CS-H. californicum amphiploid and the chromosome addition lines （DA2H and MA5H） identified were evaluated for powdery mildew （erysiphe graminis f. sp. triticii） resis- tance in field. The preliminary results indicated that the amphiploid showed higher powdery mildew resistance than CS. However, chro- mosome addition lines DA2H and MA5H were highly susceptible to powdery mildew, indicating that major powdery mildew resistant genes of H. californicum should be located on chromosomes other than 2H and 5H.

关键词： Hordeum californicum alien addition lines powdery mildew sequential C-banding/FiSH sequence-tag-sites (STS)

influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance genes between *** strains

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Frontiers of environmental Science & engineering 2020年第6期14卷 191-200页

作者： Qingkun ji Caihong Zhang Dan Li Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention(LAP3) Department of Environmental Science and EngineeringFudan UniversityShanghai 200433China Department of Obstetrics and Gynecology Changhai HospitalSecond Military Medical UniversityShanghai 200433China

The spread and development of antibiotic resistance globally have led to severe public health *** has been shown that some non-antibiotic substances can also promote the diffusion and spread of antibiotic resistance genes(ARGs).Nanofullerene(nC60)is a type of nanomaterial widely used around the world,and some studies have discovered both the biological toxicity and environmental toxicity of nC6O .in this study,cellular and molecular biology techniques were employed to investigate the influences of nC6O at sub-minimum inhibitory concentrations(sub-miCs)on the conjugation of ARGs between the *** *** with the control group,nC6O significantly increased the conjugation rates of ARGs by 1.32-10.82 folds within the concentration range of 7.03-1800μg/*** study further explored the mechanism of this phenomenon,finding that sub-miCs of nC6O could induce the production of reactive oxygen species(ROS),trigger SOSresponse and oxidative stress,affect the expression of outer membrane proteins(OMPs)genes,increase membrane permeability,and thus promote the occurrence of *** research enriches our understanding of the environmental toxicity of nC6O,raises our risk awareness toward nC6O ,and may promote the more rational employment of nC6O materials.

关键词： Nanofullerene Sub-minimum inhibitory concentrations Antibiotic resistance genes Conjugation Molecular biological techniques

Cytotoxicity of Ti–6Al–4V–5Cu Alloy to MC3T3-e1 Cells

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Acta Metallurgica Sinica(english Letters) 2021年第5期34卷 694-700页

作者： ji Zhang Ling Ren Ke Yang institute of Metal Research Chinese Academy of SciencesShenyang110016China

The aim of this work was to study the cytotoxicity of Ti–6 Al–4 V–5 Cu(TC4–Cu)alloy for dental applications and evaluate the cell viability of diff erent fabricated TC4–Cu alloys in contact with MC3 T3-e1 cells in ***–6 Al–4 V(TC4)alloy was used as a negative control to evaluate the cytotoxicity level of TC4–Cu alloy so as to provide basic support for the dental clinical *** group TC4 and experimental group TC4–Cu with diff erent fabrications were incubated in the cell culture *** absorbance value of mouse osteoblast MC3 T3-e1 cells was detected by CCK-8 assay after 1,3 and 7 days,*** relative proliferation rate of cells was calculated,and then the toxicity level was valued for each *** morphology on the surface was also studied by observing the cytoskeleton through F-actin filament *** experimental results showed that the absorbance values for the experimental and the negative control groups were not same at diff erent time *** with diff erent fabricated TC4–Cu alloys,the annealing-TC4–Cu alloy showed much better *** mouse osteoblast MC3 T3-e1 cells cultured with annealing-TC4–Cu,rolling-TC4–Cu and solution+aging-TC4–Cu have no toxic eff ects,and these alloys could promote the proliferation of mouse osteoblasts.

关键词： Cytotoxicity Ti–6Al–4V–5Cu alloy Fabrication Biocompatibility

Unified Principal S-N equation for Friction Stir Welding of 5083 and 6061 Aluminum Alloys

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Chinese Journal of Mechanical engineering 2021年第2期34卷 190-200页

作者： Xiangwei Li ji Fang Xiaoli Guan CRRC QiQiHAR ROLLiNG STOCK CO. LTDQiqihar 161000China College of Locomotive and Rolling Stock engineering Dalian Jiaotong UniversityDalian 116028China

With the popularization of friction stir welding(FSW),5083-H321 and 6061-T6 aluminum alloy materials are widely used during the FSW *** this study,the fatigue life of friction stir welding with two materials,i.e.,5083-H321 and 6061-T6 aluminum alloy,are *** tests were carried out on the base metal of these two materials as well as on the butt joints and overlapping FSW *** principle of the equivalent structural stress method is used to analyze the FSW test data of these two *** fatigue resistances of these two materials were com-pared and a unified principal S-N curve equation was *** key parameters of the unified principal S-N curve obtained by fitting,Cd is 4222.5,and h is 0.2693.A new method for an FSW fatigue life assessment was developed in this study and can be used to calculate the fatigue life of different welding forms with a single S-N *** main fatigue tests of bending and tension were used to verify the unified principal S-N curve *** results show that the fatigue life calculated by the unified mean 50%master S-N curve parameters are the closest to the fatigue test *** reliability,practicability,and generality of the master S-N curve fitting parameters were verified using the test *** unified principal S-N curve acquired in this study can not only be used in aluminum alloy materials but can also be applied to other materials.

关键词： 5083 and 6061 aluminum alloy Friction stir welding Master S-N curve Fatigue life

Vibration control of fluid-conveying pipes: a state-of-the-art review

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Applied Mathematics and Mechanics(english edition) 2023年第9期44卷 1423-1456页

作者： Hu DiNG J.C.ji Shanghai Key Laboratory of Mechanics in energy engineering School of Mechanics and Engineering ScienceShanghai UniversityShanghai 200072China Shaoxing institute of Technology Shanghai UniversityShaoxing 312074Zhejiang ProvinceChina Shanghai institute of Aircraft Mechanics and Control Shanghai 200092China School of Mechanical and Mechatronic engineering University of Technology SydneyNew South Wales 2007Australia

Fluid-conveying pipes are widely used to transfer bulk fluids from one point to another in many engineering *** are subject to various excitations from the conveying fluids,the supporting structures,and the working environment,and thus are prone to vibrations such as flow-induced vibrations and acoustic-induced *** can generate variable dynamic stress and large deformation on fluid-conveying pipes,leading to vibration-induced fatigue and damage on the pipes,or even leading to failure of the entire piping system and catastrophic ***,the vibration control of fluid-conveying pipes is essential to ensure the integrity and safety of pipeline systems,and has attracted considerable attention from both researchers and *** present paper aims to provide an extensive review of the state-of-the-art research on the vibration control of fluid-conveying *** vibration analysis of fluid-conveying pipes is briefly discussed to show some key issues involved in the vibration ***,the research progress on the vibration control of fluid-conveying pipes is reviewed from four aspects in terms of passive control,active vibration control,semi-active vibration control,and structural optimization design for vibration ***,the main results of existing research on the vibration control of fluid-conveying pipes are summarized,and future promising research directions are recommended to address the current research *** paper contributes to the understanding of vibration control of fluid-conveying pipes,and will help the research work on the vibration control of fluidconveying pipes attract more attention.

关键词： fuid-conveying pipe vibration passive control nonlinear energy sink(NeS) active control semi-active control

in-situ TeM investigation on the evolution of He bubbles and dislocation loops in Ni-based alloy irradiated by H_(2)^(+)&He^(+)dual-beam ions

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Journal of Materials Science & Technology 2023年第7期138卷 36-49页

作者： Zhenbo Zhu Weichi ji Hefei Huang Shanghai institute of Applied Physics Chinese Academy of SciencesShanghai 201800China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China

The synergistic evolution mechanisms of He bubbles and dislocation loops under 30 keV H_(2)^(+)&He^(+)dual-beam ions irradiation at 650℃ in the Ni-based alloy GH3535,which is the most promising candidate structure material for molten salt reactors(MSRs),were revealed via in-situ *** nucleation,merg-ing,and change in the size of the dislocation loops and He bubbles were characterized in detail to study the influences of irradiation fluence and pre-existing dislocation loops on their *** number density of both the He bubbles and dislocation loops increases rapidly and subsequently saturates,whereas their size continuously increases with the increasing ion ***-existing dislocation loops with strong absorption characteristics grow preferentially and suppress the nucleation of dislocation loops during the dual-beam ions ***,the bubbles tend to nucleate within the dislocation loops to form bubble-loop complexes,and then decrease in their number *** details of the un-faulting processes of the Frank loops were discussed,where the energy difference between the two types of loops as well as the evolution of the inside Shockley dislocation loops dominates the unfaulting *** several evolution stages of the loop-punching mechanism are revealed,and the emitted loops can directly form perfect loops as well as unfault the neighboring Frank *** He bubbles inside the loops provide corresponding stress for the formation of rhombic loops,which can achieve rapid growth and sweep ability by merging with the neighboring ***,its dissociation to Shockley dis-location can unfault the Frank loops along their slip *** the four Frank loop variations,the edge-on Frank loop variations have the highest growth rate,followed by the perfect *** related mechanisms based on in-situ experimental observation are discussed in depth.

关键词： in-situ TeM observation Ni-based alloy Dislocation loop Punching loop Bubble-loop complex

effects of domain unfolding and catch-like dissociation on the collective behavior of integrin-fibronectin bond clusters

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Acta Mechanica Sinica 2021年第2期37卷 229-243页

作者： ji Lin Yanzhong Wang jin Qian Department of engineering Mechanics Key Laboratory of Soft Machines and Smart Devices of Zhejiang ProvinceZhejiang UniversityHangzhou 310027China

To gain more understanding of cell-matrix adhesion,we consider an idealized theoretical model of a cluster of integrin-fibronectin bonds at the cell-matrix interface subjected to a dynamic *** distributions of bond traction and interfacial deformation are assumed to obey classical elastic equations,whereas the dissociation/association of individual bonds as well as unfolding/refolding of fibronectin domains are described by stochastic *** stochastic-elasticity coupling,we perform Monte Carlo simulations to investigate how the collective behavior and adhesion performance of the integrin-fibronectin-mediated interface are influenced by two characteristics newly incorporated in the modeling,i.e.,catchlike dissociation between integrin and fibronectin,and unfolding of repeated domains in *** probable unfolding of fibronectin domains is found to have profound effects on the resultant adhesion energy of the integrin-fibronectin-mediated interface,and governs the failure model transiting between uniform decay and catastrophic crack-like rupture.

关键词： integrin-fibronectin complex Cell-matrix adhesion Domain unfolding Catch bond Rate dependence Modulus effect

Robust Cross-Linked Na_(3)V_(2)(PO_(4))_(2)F_(3) Full Sodium-ion Batteries

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energy & environmental Materials 2024年第1期7卷 9-20页

作者： jinqiang Gao Ye Tian Lianshan Ni Baowei Wang Kangyu Zou Yingchang Yang Ying Wang Craig e.Banks Dou Zhang Kechao Zhou Huan Liu Wentao Deng Guoqiang Zou Hongshuai Hou Xiaobo ji College of Chemistry and Chemical engineering Central South UniversityChangsha 410083China College of Material and Chemical engineering Tongren UniversityTongren 554300China Department of Chemistry The Chinese University of Hong KongShatinN.T.Hong Kong S.A.R.China Faculty of Science and engineering Manchester Metropolitan UniversityManchester UK State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083China School of Materials Science and engineering Hunan Provincial Key Lab of Advanced Materials for New Energy Storage and ConversionHunan University of Science and TechnologyXiangtan 411201China School of Materials and engineering Zhengzhou UniversityZhengzhou 450001China

Sodium-ion batteries(SiBs)have rapidly risen to the forefront of energy storage systems as a promising supplementary for Lithium-ion batteries(LiBs).Na_(3)V_(2)(PO_(4))_(2)F_(3)(NVPF)as a common cathode of SiBs,features the merits of high operating voltage,small volume change and favorable specific energy ***,it suffers from poor cycling stability and rate performance induced by its low intrinsic ***,we propose an ingenious strategy targeting superior SiBs through cross-linked NVPF with multi-dimensional nanocarbon frameworks composed of amorphous carbon and carbon nanotubes(NVPF@C@CNTs).This rational design ensures favorable particle size for shortened sodium ion transmission pathway as well as improved electronic transfer network,thus leading to enhanced charge transfer kinetics and superior cycling *** from this unique structure,significantly improved electrochemical properties are obtained,including high specific capacity(126.9 mAh g^(-1)at 1 C,1 C=128 mA g^(-1))and remarkably improved long-term cycling stability with 93.9%capacity retention after 1000 cycles at 20 *** energy density of 286.8 Wh kg^(-1)can be reached for full cells with hard carbon as anode(NVPF@C@CNTs//HC).Additionally,the electrochemical performance of the full cell at high temperature is also investigated(95.3 mAh g^(-1)after 100 cycles at 1 C at 50℃).Such nanoscale dual-carbon networks engineering and thorough discussion of ion diffusion kinetics might make contributions to accelerating the process of phosphate cathodes in SiBs for large-scale energy storages.

关键词： dual-nanocarbon networks full sodium-ion battery ion transfer kinetics Na_(3)V_(2)(PO_(4))_(2)F_(3) NASiCON structure

Paleogeomorphy,provenance system and sedimentary system of the Dongying formation in the Qikou sag

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mining Science and Technology 2009年第6期19卷 800-806页

作者： WANG Hua HUANG Chuan-yan ZHAO Shu-e YAN De-tian BAi Yun-feng XiANG Xue-mei CHeN Si XiA Cun-yin LiAO ji-hua Faculty of earth Resources China University of Geosciences Wuhan Hubei 430074 China Daqing Research institute of Petroleum exploration and Development Daqing Heilongfiang 163453 China

Reconstructed synsedimentary paleogeomorpholgy is an effective method in predicting the distribution of sandbodies and can provide valid evidence in the search for reservoirs. Based on a synsedimentary paleogeomorpholgy reconstruction of the third member of the Dongying formation (ed3) in the Qikou sag,the basic paleogeomorphic characteristics of ed3 are described and the spatial distributions of denudation and subsidence areas are discussed. Key boundary faults controlling the deposition of important sediment bodies are proposed and the interrelations among faulted slope-break belts or flexure belts,intra-depression uplifts and sags,sediment sources and sediments input points have been investigated.

关键词： paleogeomorphology provenance system sedimentary system Dongying formation Qikou sag