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Genetic associations of inflammatory bowel disease in a South Asian population

World Journal of Clinical Cases 2018年第15期6卷 908-915页

作者： Madunil Anuk Niriella Isurujith Kongala Liyanage Senerath Kuleesha Kodisinghe Arjuna priyadarsin De silva Nimna Rajapakshe Sunali D Nanayakkara Dunya Luke Thilakshi silva Metthananda Nawarathne Ranjith K peiris Udaya p Kalubovila Sujeewa R Kumarasena Vajira Harshadeva Weerabaddana Dissanayake Rohan W Jayasekara Hithanadura Janaka de silva Faculty of Medicine University of Kelaniya Faculty of Medical Sciences University of Sri Jayewardenepura University Medical Unit Colombo North Teaching Hospital Gastroenterology Unit National Hospital of Sri Lanka Gastroenterology Unit Colombo South Teaching Hospital Gastroenterology Unit Teaching Hospital Kandy Gastroenterology Unit Teaching Hospital Karapitiya Human Genetics Unit Faculty of Medicine University of Colombo

AIM To estimate prevalence and phenotypic associations of selected inflammatory bowel disease(IbD)-associated genetic variants among Sri Lankan patients. METHODS A case study of histologically confirmed ulcerative colitis(UC) or Crohn's disease(CD) patients with ≥ 1 year disease duration, who were compared to unrelated, gender-matched, healthy individuals as controls, was conducted at four major centers in Sri Lanka. phenotypic data of the cases were obtained and all participants were genotyped for 16 selected genetic variants: IL12 b :rs1045431, IL23 R :rs11805303, ARpC2 :rs12612347, IRGM :rs13361189, IL26/IL22 :rs1558744, CDH1 :rs1728785, IL10 :rs3024505, FCGR2 A :rs3737240, pTGER4 :rs4613763, IL17 REL/pIM3 :rs5771069, HNF4 a :rs6017342, STAT3 :rs744166, SMURF1 :rs7809799, LAMb1 :rs886774, HLA-DRb5, DQA1, DRb1, DRA :rs9268853, MST1, UbA7, and ApEH :rs9822268. The genotypes of all variants were in Hardy-Weinberg Equilibrium(p > 10^(-3)). To account for multiple hypothesis testing, p-values patients and 465 controls were recruited. Out of the single nucleotide polymorphisms(SNps) tested, the majority were not associated with IbD in Sri Lankans. Significant positive associations were noted between rs886774(LAMb1-gene) and UC(odds ratio(OR) = 1.42, p = 0.001). UC patients with rs886774 had mild disease(OR = 1.66, p p positive association was noted between rs10045431(IL 12 b gene) and upper gastrointestinal involvement in CD(OR = 4.76, p = 0.002). CONCLUSION This confirms the heterogeneity of allelic mutations in South Asians compared to Caucasians. Most SNps and disease associations reported here have not been described in South Asians.

关键词： Inflammatory bowel disease Genetics of inflammatory bowel disease Ulcerative colitis Crohn’s disease LAMb1 gene mutation IL-12b gene mutation

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Ion dynamics into different pore size distributions in supercapacitors under compression

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Journal of Energy Chemistry 2023年第5期80卷 110-119,I0004页

作者： João pedro Aguiar dos Santos Cesar J.b.p.gan Rafael Vicentini Reinaldo F.Teófilo Renato beraldo Leonardo M.Da silva Hudson Zanin brazilian Renewable Energies Center for Innovation on New EnergiesSchool of Electrical and Computer EngineeringUniversity of CampinasAv.Albert Einstein 40CampinasSP13083-852Brazil School of Electrical and Computer Engineering University of CampinasSP 13083-852Brazil Multivariate Chemical Data Analysis Laboratory Department of ChemistryUniversidade Federal de Viçosa36570-900 ViçosaMGBrazil Department of Chemistry Laboratory of Fundamental and Applied ElectrochemistryFederal University of Jequitinhonha and Mucuri's ValleyRodovia MGT 367km 5835000 Alto da Jacuba39100-000DiamantinaMGBrazil

Compressing supercapacitor(SCs)electrode is essential for improving the energy storage characteristics and minimizing ions’distance travel,faradaic reactions,and overall ohmic *** comprising the ion dynamics in SC electrodes under compression are still ***,the ionic dynamics of five aqueous electrolytes in electrodes under compression were studied in this work for tracking electrochemical and structural changes under mechanical stress.A superionic state is formed when the electrode is compressed until the micropores match the dimensions with the electrolyte’s hydrated ion sizes,which increases the *** excessive compression is applied,the accessible pore regions decrease,and the capacitance ***,as the studied hydrated ions have different dimensions,the match between ion/pore sizes *** the LiOH and NaClO4electrolytes,increasing the pressure from 60 to 120 and 100 pSI raised the capacitance from 13.5 to 35.2 F g^(-1)and 30.9 to 39.0 F g^(-1),***,the KOH electrolyte with the lowest and LiCl with the biggest combination of hydrated ion size have their point of maximum capacitance(39.5 and 36.7F g^(-1))achieved at 140 and 80 pSI,*** LiCl and KCl electrolytes,overcompression causes a drop in capacitance higher than 23%.

关键词： Mechanic compression pore size distribution lon dynamics Activated carbon Supercapacitors

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Ancient DNA of the pygmy marmoset type specimen Cebuella pygmaea(Spix,1823)resolves a taxonomic conundrum

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Zoological Research 2021年第6期42卷 761-771页

作者： Jean p.boubli Mareike C.Janiak Leila M.porter Stella de la Torre Liliana Cortes-Ortiz Maria N.Fda silva Anthony b.Rylands Stephen Nash Fabricio bertuol Hazel byrne Felipe E.silva Fabio Rohe Dorien de Vries Robin M.D.beck Irune Ruiz-Gartzia Lukas F.K.Kuderna Tomas Marques-bonet Tomas Hrbek Izeni p.Farias Anneke Hvan Heteren Christian Roos School of Science Engineering&EnvironmentUniversity of SalfordSalford M54WTUK Instituto Nacional de pesquisas da Amazonia ManausAmazonas 69060-001Brazil Department of Anthropology Northern Illinois UniversityDeKalbIL 60115-2828USA College of biological and Environmental Sciences Universidad San Francisco de QuitoQuito 170901Ecuador Department of Ecology and Evolutionary biology University of MichiganAnn ArborMI 48109USA Re:wild AustinTX 78767USA Laboratorio de Evolução e Genética Animal Universidade Federal do AmazonasManausAmazonas 69080-900Brazil Department of Anthropology University of UtahSalt Lake CityUT 84112USA Research Group on primate biology and Conservation MamirauaInstitute for Sustainable DevelopmentTeféAmazonas 69553-225Brazil Experimental and Health Sciences Department(DCEXS) Institut de Biologia EvolutivaUniversitat Pompeu Fabra-CSICBarcelona 08002Spain Department of biology Trinity UniversitySan AntonioTX 78212USA bavarian State Collection of Zoology Zoologische Staatssammlung MünchenStaatliche Naturkundliche Sammlungen Bayerns81247 MunichGermany Geobio-Center Ludwig-Maximilians-Universitat München80333 MunichGermany Department biologie II Ludwig-Maximilians-Universitat München82152 MunichGermany Gene bank of primates and primate Genetics Laboratory German Primate CenterLeibniz Institute for Primate Research37077 GottingenGermany

The pygmy marmoset,the smallest of the anthropoid primates,has a broad distribution in Western *** studies using molecular and morphological data have identified two distinct species separated by the Napo and Solimoes-Amazonas ***,reconciling this new biological evidence with current taxonomy,i.e.,two sub.p.cies,Cebuella pygmaea pygmaea(Spix,1823)and Cebuella pygmaea niveiventris(Lönnberg,1940),was problematic given the uncertainty as to whether Spix’s pygmy marmoset(Cebuella pygmaea pygmaea)was collected north or south of the Napo and Solimoes-Amazonas rivers,making it unclear to which of the two newly revealed species the name pygmaea would ***,we present the first molecular data from Spix’s type specimen of Cebuella pygmaea,as well as novel mitochondrial genomes from modern pygmy marmosets sampled near the type locality(Tabatinga)on both sides of the *** these data,we can confirm the correct names of the two species identified,i.e.,*** for animals north of the Napo and Solimoes-Amazonas rivers and *** for animals south of these two *** analyses of the novel genetic data placed into the context of cytochrome b gene sequences from across the range of pygmy marmosets further led us to reevaluate the geographical distribution for the two Cebuella *** dated the split of these two species to 2.54 million years *** discuss additional,more recent,subdivisions within each lineage,as well as potential contact zones between the two species in the headwaters of these rivers.

关键词： Historic DNA DNA taxonomy pygmy marmoset Cebuella pygmaea C.niveiventris Amazon Type specimen

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Effectiveness of bNT162b2 and CoronaVac vaccines against omicron in children aged 5 to 11 years

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World Journal of pediatrics 2023年第10期19卷 949-960页

作者： Eduardo A.Oliveira Maria Christina L.Oliveira Ana Cristina Simões e silva Enrico A.Colosimo Robert H.Mak Mariana A.Vasconcelos Ludmila R.silva Daniella b.Martelli Clara C.pinhati Hercílio Martelli-Júnior Department of pediatrics Health Sciences Postgraduate ProgramSchool of MedicineFederal University of Minas Gerais(UFMG)R.Engenheiro Amaro Lanari 389/501Belo HorizonteMG30130-100Brazil Department of Statistics Federal University of Minas GeraisBelo HorizonteMGBrazil Department of pediatrics Rady Children’s HospitalUniversity of CaliforniaLa JollaSan DiegoCAUSA Health Science/postgraduate program in Nursing.School of Nursing Federal University of Minas Gerais(UFMG)Belo HorizonteMG30130-100Brazil Health Science/primary Care postgraduate program State University of Montes Claros(Unimontes)Montes ClarosMG39401-089Brazil

background This study aimed to estimate vaccine effectiveness(VE)against omicron variant infection and severe corona virus disease 2019(COVID-19)in children aged 5–11 years hospitalized with acute respiratory *** A test-negative,case–control analysis was conducted from February 2022 to June *** enrolled 6950 eligible children,including 1102 cases and 5848 *** was calculated after immunization with one and two doses of bNT162b2 or *** outcomes were hospitalization with acute respiratory symptoms and detection of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection and severe *** adjusted odds ratio for the association of prior vaccination and outcomes was used to estimate *** For fully vaccinated children,the overall estimated VE against hospitalization with SARS-CoV-2 infection was 42%[95%confidence interval(CI)26 to 54].VE peaked at 29–42 days(67%,95%CI 40%to 82%)and then declined to 19%(95%CI,−20%to 45%)at 57–120 days after the second *** bNT162b2 vaccine had a similar VE against hospitalization with SARS-CoV-2 infection(45%,95%CI,20 to 61)compared to the CoronaVac vaccine(40%,95%CI,17%to 56%).Among cases,56(5%)children died;53(94.6%)were not fully *** cases,the two-dose schedule effectiveness against ICU admission,need for invasive ventilation,severe illness,and death were 10%(95%CI,−54%–45%),22%(95%CI−70%–68%),12%(95%CI,−62%–52%),and 16%(95%CI,−77%–75%),*** For hospitalized children aged 5–11 years during the omicron-predominant period in brazil,two doses of both vaccines had moderate effectiveness against hospitalization with acute respiratory symptoms and SARS-CoV-2 infection and offered limited protection against endpoints of COVID-19 severity.

关键词： Children COVID-19 Effectiveness Omicron Vaccine

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In vitro antileishmanial effects of physalis angulata root extract on Leishmania infantum

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Journal of Integrative Medicine 2018年第6期16卷 404-410页

作者： bruno Jose Martins Da silva Sandro Wilson Gomes pereira Ana paula Drummond Rodrigues Jose Luiz Martins Do Nascimento Edilene Oliveira silva Laboratory of Structural biology Institute of Biological SciencesFederal University of PardBelemPard 66075-110Brazil National Institute of Science and Technology in Structural biology and bioimaging(INCT-INbEb) Rio de Janeiro 21941-901Brazil Laboratory of Electron Microscopy Department of Health SurveillanceMinistry of HealthEvandro Chagas InstituteBelemPard 66087-082Brazil Laboratory of Molecular and Cellular Neurochemistry Institute of Biological SciencesFederal University of PardBelemPard 66075-110Brazil National Institute of Science and Technology in Neuroimmunomodulation(INCT-NIM) Rio de Janeiro 21040-360Brazil

Objective： In the present study, we evaluated the effects of the aqueous extract of physalis angulata root （AEpa） on Leishmania infanturn proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. Methods： I., infantum promastigotes were treated with 50 and 100 μg/mL AEpa for 72 h and then antipro- mastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEpa and effects of AEpa on cytokine secretion after 72-hour treatment. Results： Treatment with AEpa reduced the numbers ofL infantum promastigotes （50% inhibitory concen- tration （ICso） = 65.9μg/mL; selectivity index （SI）= 22.1） and amastigotes （ICso = 37.9 μg/mL; SI = 38.5） compared with the untreated control. Amphotericin b reduced 100% of the promastigote numbers after 72 h of treatment （IC50 = 0.2μg/mL）. AEpa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEpa （100 μg/mL） promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEpa showed no cytotoxic effect on J774-A1 cells （50% cytotoxic concentration 〉1000μg/mL）. Conclusion： AEpa presented antileishmanial activity against the promastigotes and amastigotes of I.. infantum without macrophage cytotoxicity; these results show that natural products such as p. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis.

关键词： Leishmanicidal activity Leishmania infantum Reactive oxygen species Apoptosis physalis angulata

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Structural,microstructural and dielectric characterizations of(bi_(0.5)Na_(0.5))TiO_(3) based lead-free ferroelectric ceramics

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Journal of Advanced Dielectrics 2021年第3期11卷 39-44页

作者： b.R.Moya A.C.silva A.peláiz-barranco J.D.S.Guerra Grupo de Ferroelétricos e Materiais Multifuncionais Instituto de Física Universidade Federal de Uberlandia UberlandiaMinas Gerais 38408-100Brazil Departamento de Física e Química UNESP Campus de Ilha SolteiraSao Paulo 15385-000Brazil Grupo de Materiales Ferroicos Facultad de Física-Instituto de Ciencia y Tecnología de Materiales Universidad de La Habana La Habana 10400Cuba

(1-x)(bi_(0.5)Na_(0.5))TiO_(3)-xbaTiO_(3) lead-free ceramics have been obtained from the conventional solid-state reaction sintering *** structural properties were investigated from X-ray diffraction and Raman spectroscopy *** revealed well-crystallized ceramic samples with perovskite *** properties,obtained from scanning electron microscopy measurements,have shown high density with very low porosity *** dielectric response,analyzed as a function of the temperature and several frequencies,showed very broad peaks with a strong frequency dependence of the temperature for the maximum dielectric permittivity for the modified *** were analyzed considering the influence of the baTiO3 content on the studied physical properties.

关键词： Lead-free bNT Raman spectroscopy dielectric response

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Central nervous system vasculitis and polyneuropathy as first manifestations of hepatitis C

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World Journal of Gastroenterology 2012年第2期18卷 188-191页

作者： Roberto J Carvalho-Filho Janaína Luz Narciso-Schiavon Luciano HL Tolentino Leonardo L Schiavon Maria Lucia G Ferraz Antonio Eduardo b silva Division of Gastroenterology Hepatitis SectionFederal University of Sao Paulo04037-003 Sao PauloBrazil

Sensory or motor peripheral neuropathy may be observed in a significant proportion of hepatitis C virus (HCV)-infected ***,central nervous system (CNS) involvement is uncommon,especially in cryoglobulin-negative *** describe a case of peripheral neuropathy combined with an ischemic CNS event as primary manifestations of chronic HCV infection without *** improvement was observed after antiviral *** discuss the spectrum of neurological manifestations of HCV infection and review the literature.

关键词： Hepatitis C Central nervous system poly-neuropathy Interferon-α

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Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering

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bio-Design and Manufacturing 2019年第4期2卷 225-241页

作者： Joao bCosta Joana silva-Correia Sandra pina Alain da silva Morais Sílvia Vieira Hélder pereira Joao Espregueira-Mendes Rui LReis Joaquim M.Oliveira b′s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkZona Industrial da Gandra4805-017 Barco GmrPortugal ICVS/3b’s-pT Government Associate Laboratory Braga/GuimaraesPortugal The Discoveries Centre for Regenerative and precision Medicine Headquarters at University of MinhoAvepark4805-017 BarcoGuimar'sPortugal Ripoll y De prado Sports Clinic:Murcia-Madrid FIFA Medical Centre of Excellence MurciaSpain Orthopedic Department Centro Hospitalar póvoa de Varzim Vila do CondePortugal Clínica do Dragao Espregueira-Mendes Sports CentreFIFA Medical Centre of ExcellencePortoPortugal Dom Henrique Research Centre PortoPortugal Orthopedic Department University of MinhoBragaPortugal

The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCp) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 Mpa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 Mpa. by its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 Mpa and dynamical mechanical properties ranging from 1.03 ± 0.08 Mpa to 1.56 ± 0.13 Mpa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCp and SF16/TCp) of the hierarchical sca ffolds Hi8 and Hi16, respectively. both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patie

关键词： patient-specific Indirect printing Hierarchical Silk fibroin Enzymatic-cross-linking Meniscus

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The role of airborne particles and environmental considerations in the transmission of SARS-CoV-2

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Geoscience Frontiers 2021年第5期12卷 1-16页

作者： Longyi Shao Shuoyi Ge Tim Jones M.Santosh Luis F.O.silva Yaxin Cao Marcos L.S.Oliveira Mengyuan Zhang Kelly bérubé State Key Laboratory of Coal Resources and Safe Mining China University of Mining and Technology(Beijing)Beijing 100083China College of Geoscience and Surveying Engineering China University of Mining and Technology(Beijing)Beijing 100083China School of Earth and Environmental Sciences Cardiff UniversityMuseum AvenueCardiffCF103YEUK School of Earth Sciences and Resources China University of Geosciences BeijingBeijing 100083China Department of Earth Science University of AdelaideAdelaideSA 5005Australia Department of Civil and Environmental Universidad de la CostaCalle 58#55-66080002 BarranquillaAtlánticoColombia Departamento de Ingeniería Civil y Arquitectura Universidad de LimaAvenida Javier Prado Este 4600-Santiago deSurco 1503Peru School of biosciences Cardiff UniversityMuseum AvenueCardiff CF103AXWalesUK

Corona Virus Disease 2019(COVID-19)caused by the novel coronavirus,results in an acute respiratory condition coronavirus 2(SARS-CoV-2)and is highly *** recent spread of this virus has caused a global ***,the transmission routes of SARS-CoV-2 are being established,especially the role of environmental *** we review the environmental transmission routes and persistence of *** studies have established that the transmission of this virus may occur,amongst others,in the air,water,soil,cold-chain,biota,and surface *** has also been found that the survival potential of the SARS-CoV-2 virus is dependent on different environmental conditions and *** important pathways include aerosol and fecal *** matter may also be a carrier for *** microscopic particles can be easily absorbed by humans,more attention must be focused on the dissemination of these *** considerations are required to evolve a theoretical platform for epidemic control and to minimize the global threat from future epidemics.

关键词： Aerosol Airborne particles COVID-19 Environmental media SARS-CoV-2

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Hepatocellular carcinoma in Latin America: Diagnosis and treatment challenges

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World Journal of Gastroenterology 2018年第37期24卷 4224-4229页

作者： Federico pinro Jaime poniachik Ezequiel Ridruejo Marcelo silva Hospital Universitario Austral Facultad de Ciencias BiomédicasUniversidad AustralPilarBuenos Aires B1629HJArgentina American Liver Research Educational and Awareness Network Buenos Aires B1629HJArgentina Hepatology Hospital Clínico Universidad de ChileSantiago de ChileRegión Metropolitana Santiago 46010Chile Santa María Santiago de ChileRegión Metropolitana Santiago 834-0518Chile Médica e Investigaciones Clínicas Norberto Quirno Ciudad de Buenos Aires C1425ASGArgentina

Latin America, a region with a population greater than 600000000 individuals, is well known due to its wide geographic, socio-cultural and economic heterogeneity. Access to health care remains as the main barrier that challenges routine screening, early diagnosis and proper treatment of hepatocellular carcinoma(HCC). Therefore, identification of population at risk, implementation of surveillance programs and access to curative treatments has been poorly obtained in the region. Different retrospective cohort studies from the region have shown flaws in the implementation process of routine surveillance and early HCC diagnosis. Furthermore, adherence to clinical practice guidelines recommendations assessed in two studies from brazil and Argentina demonstrated that there is also room for improvement in this field, similarly than the one observed in Europe and the United States. In summary, Latin America shares difficulties in HCC decision-making processes similar to those from developed countries. However, a transversal limitation in the region is the poor access to health care with the consequent limitation to standard treatments for overall population. Specifically, universal health care access to the different World Health Organization levels is crucial, including improvement in research, education and continuous medical training in order to expand knowledge and generation of data promoting a continuous improvement in the care of HCC patients.

关键词： Latin America Liver cancer Limitations Challenge

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