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High-throughput extraction on a dynamic solid phase for low-abundance biomarker isolation from biological samples

Microsystems & Nanoengineering 2023年第5期9卷 101-112页

作者： Lucile Alexandre Monica Araya-Farias Manh-Louis Nguyen Nikoletta Naoumi giacomo Gropplero Electra Gizeli Laurent Malaquin Stéphanie Descroix Laboratoire Physico-Chimie Curie CNRS UMR 168Institut CuriePSL Research UniversityParisFrance Institut Pierre-Gilles de Gennes(IPGG) Sorbonne UniversityParisFrance Department of Biology University of CreteHeraklionGreece Institute of Molecular Biology and Biotechnology(IMBB)-FORTH HeraklionGreece Laboratoire d’analyse et d’architecture des systèmes(LAAS)CNRS Elia GroupToulouseFrance Present address:Frédéric Joliot Institute for Life Sciences Pharmacology and Immunoanalysis UnitImmunoanalysis Studies and Research LaboratoryAlternative Energies and Atomic Energy Commission(CEA)Gif-sur-YvetteFrance

Liquid biopsy,in particular circulating tumor DNA(ctDNA)analysis,has paved the way for a new noninvasive approach to cancer diagnosis,treatment selection and *** a crucial step in the analysis,the extraction of the genetic material from a complex matrix needs to meet specific requirements such as high specificity and low loss of ***,we developed a new generation of microfluidic fluidized beds(FBs)that enable the efficient extraction and preconcentration of specific ctDNA sequences from human serum with flow rates up to 15µL/*** first demonstrated that implementation of a vibration system inducing flow rate fluctuations combined with a mixture of different bead sizes significantly enhanced bead homogeneity,thereby increasing capture *** advantage of this new generation of high-throughput magnetic FBs,we then developed a new method to selectively capture a double-stranded(dsDNA)BRAF mutated DNA sequence in complex matrices such as patient ***,as proof of concept,ligation chain reaction(LCR)assays were performed to specifically amplify a mutated BRAF sequence,allowing the detection of concentrations as low as 6×104 copies/µL of the mutated DNA sequence in serum.

关键词： diagnosis specificity thereby

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Chloroquine reduces Th17 cell differentiation by stimulating T-bet expression in T cells

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Cellular & Molecular Immunology 2021年第3期18卷 779-780页

作者： Rodolfo Thome Alexandra Boehm Larissa Lumi Watanabe Ishikawa giacomo Casella Jaqueline Munhoz Bogoljub Ciric Guang-Xian Zhang Abdolmohamad Rostami Department of Neurology Thomas Jefferson UniversityPhiladelphiaPAUSA

Chloroquine(CQ)is a weak base that was originally used to treat malaria infection.1 However,recent findings have pinpointed the modulatory effect of CQ in models of chronic inflammation and viral *** antiviral effect of CQ and its derivative hydroxychloroquine has attracted great attention due to the recent Sars-CoV-2 virus outbreak.

关键词： Th17 stimulating inflammation

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Ecology of Artificial ***,Education and Health

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Sociology Study 2023年第3期13卷 116-125页

作者： giacomo Buoncompagni University of Florence FlorenceItaly

The ecology of Artificial Intelligence could be an interdisciplinary field of study that takes as its object the expressive forms of human culture,starting from its systemic *** to this approach,as in the study of‘old and new’media,AI could also be conceived as an environment capable of influencing human perception and knowledge,emotions and values,a space in which individual and social experiences can take *** following reflection attempts to reflect on a rich and complex topic from a historical and scientific point of view:that of Artificial ***,the characteristics and role of AI in contemporary society will be considered,as well as the implications and links of this new technology,capable of imitating human intelligence,on media,education and *** what we call(perhaps erroneously)AI become our cognitive/communicative and social collaboration environment?

关键词： AI digital culture relation education health

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ISC-GEM地震目录的产出

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地球物理学进展 2019年第5期34卷 1770-1775页

作者：李保昆冯向东万永革 Domenico di giacomo 黄辅琼马延路中国地震台网中心北京100045 河北省地震局石家庄050021 防灾科技学院河北燕郊101601 国际地震中心英国萨彻姆RG194NS

ISC-GEM地震目录是ISC发布和管理的一份全新的、基于仪器记录的全球中强震地震目录,于2013年首次发布.截至2018年2月,该目录从1900-2014年,跨越115年的时间尺度,共计28000余条地震,其中中国陆地区域的地震近千条.ISC-GEM地震目录的产出... 详细信息

ISC-GEM地震目录是ISC发布和管理的一份全新的、基于仪器记录的全球中强震地震目录,于2013年首次发布.截至2018年2月,该目录从1900-2014年,跨越115年的时间尺度,共计28000余条地震,其中中国陆地区域的地震近千条.ISC-GEM地震目录的产出过程中,收集了1900年以后共百余年的观测数据,截至2018年6月,可用的地震波到时数据达20000000余条,振幅数据达6000000余条.目录中的所有地震经过两步法重新定位:EHB方法确定震源深度,ISCloc修定震中和发震时间.矩震级是唯一的震级标度,采用直接计算或由其他震级(M_S和m_b)转换的方式而获得.

关键词： ISC-GEM 地震目录

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Spatiotemporal oxygen dynamics in young leaves reveal cyclic hypoxia in plants

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Molecular Plant 2024年第3期17卷 377-394页

作者： Paolo M.Triozzi Luca Brunello giacomo Novi Gianmarco Ferri Francesco Cardarelli Elena Loreti Mariano Perales Pierdomenico Perata PlantLab Center of Plant SciencesSant'Anna School of Advanced Studies56010 PisaItaly Centro de Biotecnologia y Genomica de Plantas Universidad Politecnica de Madrid(UPM)-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria(INIA-CSIC)Campus de Montegancedo UPMPozuelo de Alarcon28223 MadridSpain Fondazione Pisana per la Scienza 56017 PisaItaly Laboratorio NEST Scuola Normale SuperioreIstituto Nanoscienze-CNRPiazza S.Silvestro1256127 PisaItaly institute of Agricultural Biology and Biotechnology National Research Council56124 PisaItaly Departamento de Biotecnologia-Biologia Vegetal Escuela Tecnica Superior de Ingenieria AgronomicaAlimentaria y de BiosistemasUniversidad Politecnica de Madrid(UPM)28040 Madrid.Spain

Oxygen is essential for plant growth and *** occurs in plants due to limited oxygen avail-abilityfollowing adverse environmental conditions as well inhypoxic niches in otherwisenormoxic ***,the existence and functional integration of spatiotemporal oxygen dynamics with plant development remains *** animal systems dynamic fluctuations in oxygen availability are known as cyclic *** this study,we demonstrate that cyclic fluctuations in internal oxygen levels occur in young emerging leaves of Arabidopsis *** hypoxia in plants is based on a mechanism requiring the ETHYLENE RESPONSE FACTORS type VII(ERFVII)that are central components of the oxygen-sensing machinery in *** ERFVll-dependent mechanism allows precise adjustment of leaf growth in response to carbon status and oxygen availability within plant *** study thus establishes a functional connection between internal spatiotemporal oxygen dynamics and developmental processes of plants.

关键词： Arabidopsis hypoxia cyclic hypoxia leaf development oxygen-sensing mechanism ERFVII

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Effect of orthokeratology on precision and agreement assessment of a new sweptsource optical coherence tomography biometer

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Eye and Vision 2020年第1期7卷 119-127页

作者： Bao Shu Fangjun Bao giacomo Savini Weicong Lu Ruixue Tu Haisi Chen Benhao Song Qinmei Wang Jinhai Huang School of Ophthalmology and Optometry and Eye Hospital Wenzhou Medical University270 West Xueyuan RoadWenzhou 325027ZhejiangChina Key Laboratory of Vision Science Ministry of HealthWenzhouZhejiangPeople’s Republic of China G.B.Bietti Foundation IRCCS RomeItaly

Background:To evaluate the effect of orthokeratology on precision of measurements in children using a new swept-source optical coherence tomography(SS-OCT)optical biometer(OA-2000),and agreement between its measurements and those provided by the commonly used IOLMaster based on partial coherence interferometry(PCI).Methods:This study recruited fifty-one eyes of 51 normal children(8–16 years).An operator took measurements with the two ***,a second operator took measurements with the SS-OCT *** orthokeratology was performed for one month,the same operators repeated the same *** length(AL),mean keratometry(Km)at 2.5 mm and 3.0 mm diameters(Km2.5 and Km3.0),central corneal thickness(CCT),anterior chamber depth(ACD),lens thickness(LT)and corneal diameter(CD)were ***:With the SS-OCT optical biometer,the test-retest repeatability of AL measurements was*** 95%limits of agreement of difference between the two devices for CD parameter were up to 1.53 *** orthokeratology,the fluctuation ranges of difference for Km3.0 measurement was 1.11 times higher than before orthokeratology,while the absolute values of difference for AL,Km2.5,ACD and CD measurements were ***:Before and after orthokeratology,the SS-OCT biometer showed high repeatability and reproducibility for all *** orthokeratology contact lenses affected the agreement between SS-OCT and PCI biometers for Km3.0 *** CD measurement showed poor agreement between the two devices.

关键词： Swept source optical coherence tomography Orthokeratology Precision Agreement Children

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digital assets rights management through smart legal contracts and smart contracts

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Blockchain(Research and Applications) 2023年第3期4卷 64-73页

作者： Enrico Ferro Marco Saltarella Domenico Rotondi Marco Giovanelli giacomo Corrias Roberto Moncada Andrea Cavallaro Alfredo Favenza Links Foundation 10138 TurinItaly FINCONS SpA 70132 BariItaly freelance consultant FINCONS GROUP AG Lugano6900 Switzerland

Intellectual property rights(IPR)management needs to evolve in a digital world where not only companies but also many independent content creators contribute to our culture with their art,music,and *** this respect,blockchain has recently emerged as a promising infrastructure,providing a trustworthy and immutable environment through the use of smart contracts,which may enable more agile management of digital rights and streamline royalty ***,no widespread consensus has been reached on the ability of this technology to adequately manage and transfer *** paper presents an innovative approach to digital rights management developed within the scope of an international research endeavour co-financed by the European Commission named *** approach proposes the combined usage of smart legal contracts and blockchain smart contracts to take care of the legally binding contractual aspects of IPR and,at the same time,the need for notarization,rights transfer,and royalty *** work being conducted represents a contribution to advancing the current literature on IPR management that may lead to an improved and fairer monetization process for content creators as a means of individual empowerment.

关键词： Blockchain Consumer protection Contract law Copyright protection Intellectual property Intellectual property rights(IPR) Non-fungible token(NFT) Smart legal contract Smart contract Tokenomics

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Synovial chondromatosis of the foot: Two case reports and literature review

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World Journal of Orthopedics 2019年第11期10卷 404-415页

作者： Luca Monestier giacomo Riva Placido Stissi Mahfuz Latiff Michele Francesco Surace Orthopedic and Trauma Unit ASST Settle LaghiVarese 21100Italy Residency Program in Orthopedics and Trauma University of InsubriaVarese 21100Italy Interdisciplinary Research Centre for Pathology and Surgery of the Musculoskeletal System Department of Biotechnology and Life SciencesUniversity of InsubriaVarese 21100Italy

BACKGROUND Primary synovial chondromatosis(PSC) is a rare arthropathy of the synovial joints characterized by the formation of cartilaginous nodules, which may detach and become loose bodies within the joint and may undergo secondary proliferation. PSC of the foot and ankle is exceedingly rare, with only a few cases reported in the literature. The diagnosis may be difficult and delayed until operative treatment, when it is confirmed by histological assessment. PSC may degenerate into chondrosarcoma. Operative treatment is the gold standard aiming to minimize pain, improve function, prevent or limit progression of arthritis. Surgical treatment consists in debridement by arthrotomic or arthroscopic management, but there is no consensus in the literature about timing of surgery and surgical technique. Thus, the aim of this study is to report the outcomes of the surgical treatment of two cases, together with a literature *** SUMMARY We report two cases of patients affected by PSC of the foot in stage III, according to the Milgram classification: the former PSC localized in the ankle that underwent open surgery consisted of loose bodies removal;the latter in the subtalar joint, and the choice of treatment was the arthrotomy and debridement from loose bodies, in addition to the subtalar arthrodesis. Both patients returned to complete daily and working life after *** Synovial chondromatosis is a rare benign pathology, even rarer in the ankle joint and especially in the foot. Surgery should be minimal in patients with ankle PSC,choosing the correct timing, waiting if possible until stage III. More aggressive and early surgery should be performed in patients with PSC of the foot,particularly the subtalar joint, due to the high risk of arthritic evolution.

关键词： Synovial chondromatosis Foot Ankle Review Treatment Case report

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Testicular parenchymal abnormalities in Klinefelter syndrome： a question of cancer？ Examination of 40 consecutive patients

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Asian Journal of Andrology 2015年第1期17卷 154-158,I0012页

作者： giacomo Accardo Gianfranco Vallone Daniela Esposito Filomena Barbato Andrea Renzullo Giovanni Conzo Giovanni Docimo Katherine Esposito Daniela Pasquali Department of Cardiothoracic and Respiratory Sciences Endocrine Unit Department of Biomorphological and Functional Sciences Second University of Naples Naples Italy Department of Clinical and Experimental Medicine Second University of Naples Naples Italy.

Klinefelter syndrome （KS） is a hypergonadotropic hypogonadism characterized by a 47, XXY karyotype. The risk of testicular cancer in KS is of interest in relation to theories about testicular cancer etiology generally; nevertheless it seems to be low. We evaluated the need for imaging and serum tumor markers for testicular cancer screening in KS. Participants were 40 consecutive KSpatients, enrolled from December 2009 to January 2013. Lactate dehydrogenase （LDH）, alpha-fetoprotein （AFP）, and beta-human chorionic gonadotrophin subunit （^-HCG） serum levels assays and testicular ultrasound （US） with color Doppler, were carried out at study entry, after 6 months and every year for 3 years. Abdominal magnetic resonance （MR） was performed in KS when testicular US showed micro-calcifications, testicular nodules and cysts. Nearly 62% of the KS had regular testicular echotexture, 37.5% showed an irregular echotexture and 17.5% had micro-calcifications and cysts. Eighty seven percent of KS had a regular vascular pattern, 12.5% varicocele, 12.5% nodules 〈1 cm, but none had nodules 〉1 cm. MR ruled out the diagnosis of cancer in all KS with testicular micro calcifications, nodules and cysts. No significant variations in LDH, AFP, and ^-HCG levels and in US pattern have been detected during follow-up. We compared serum tumor markers and US pattern between KS with and without cryptorchidism and no statistical differences were found. We did not find testicular cancer in KS, and testicular US, tumor markers and MR were, in selected cases, useful tools for correctly discriminating benign from malignant lesions.

关键词： abdomen magnetic resonance alpha-fetoprotein beta-human chorionic gonadotrohin subunit klinefelter syndrome lactate dehydrogenase testicular ultrasound

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Markers of acute rejection and graft acceptance in liver transplantation

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World Journal of Gastroenterology 2015年第4期21卷 1061-1068页

作者： giacomo Germani Kryssia Rodriguez-Castro Francesco Paolo Russo Marco Senzolo Alberto Zanetto Alberto Ferrarese Patrizia Burra Multivisceral Transplant Unit Department of SurgeryOncology and GastroenterologyPaduaUniversity Hospital35128 PaduaItaly

The evaluation of the immunosuppression state in liver transplanted patients is crucial for a correct posttransplant management and a major step towards the personalisation of the immunosuppressive therapy. However, current immunological monitoring after liver transplantation relies mainly on clinical judgment and on immunosuppressive drug levels, without a proper assessment of the real suppression of theimmunological system. Various markers have been studied in an attempt to identify a specific indicator of graft rejection and graft acceptance after liver transplantation. Considering acute rejection, the most studied markers are pro-inflammatory and immunoregulatory cytokines and other proteins related to inflammation. However there is considerable overlap with other conditions, and only few of them have been validated. Standard liver tests cannot be used as markers of graft rejection due to their low sensitivity and specificity and the weak correlation with the severity of histopathological findings. Several studies have been performed to identify biomarkers of tolerance in liver transplanted patients. Most of them are based on the analysis of peripheral blood samples and on the use of transcriptional profiling techniques. Amongst these, NK cell-related molecules seem to be the most valid marker of graft acceptance, whereas the role CD4+CD25+Foxp3+ T cells has still to be properly defined.

关键词： Liver transplantation Acute cellular rejection Tol

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