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Current Status of <i>M. hyopneumoniae</i>in Pigs in Grenada, West Indies

Open Journal of Veterinary Medicine 2017年第7期7卷 77-84页

作者： Traci M. Vogler Sachin Kumthekar Keshaw Tiwari claude de allie Ravindra Nath Sharma department of Pathobiology School of Veterinary Medicine St. George’s University Grenada West Indies

Mycoplasma hyopneumoniae, cause of enzootic pneumonia is known for serious economic losses in pigs. The disease is prevalent all over the world. There is no published report on Mycoplasma hyopneumoniae incidence in Grenadian pigs. The aim of this study was to estimate seroprevalence of antibodies for Mycoplasma hyopneumoniae in non vaccinated pigs in Grenada. Sera were collected randomly from 459 pigs of all ages from all six parishes of Grenada. Sera were tested for antibodies to Mycoplasma hyopneumoniae using an indirect Enzyme Linked Immunosorbant Assay (ELISA) kit. Antibodies to Mycoplasma hyopneumoniae were found in 8.71% (95% CI: 0.0644 to 0.1167) pigs. The greatest percent of positives (62.5%) (95% CI: 0.4699 to 0.7582) were in youngest group 22.5% (95% CI: 0.1211 to 0.3771) in >2 year. Positive females were overrepresented compared to males by 3:1. This is the first report on the sero-prevalence of Mycoplasma hyopneumoniae in pigs from Grenada, West Indies.

关键词： Grenada Mycoplasma hyopneumoniae Pigs Seroprevalence

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Seroprevalence of Bluetongue Virus Antibody in Ruminants from Grenada

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Open Journal of Veterinary Medicine 2016年第6期6卷 99-103页

作者： Ravindra Nath Sharma Sureni Beckford Keshaw Tiwari Elodie Vinet derek Thomas claude de allie Alfred Chikweto School of Veterinary Medicine St. George’s University St. George’s Grenada Ecole Nationale Vererinaire de Toulouse Toulouse France Veterinary Section Ministry of Agriculture Fisheries Land and Environment Grenada

The aim of the present study was to estimate the seroprevalence of antibodies to bluetongue virus (BTV) among domestic ruminants of Grenada. Sera samples from cattle (133), goat (314) and sheep (481) were tested using competitive Enzyme-linked immunosorbent assay (c-ELISA). Of the total of 928 samples tested, the overall BTV seroprevalence was 78.4% (95% confidence interval (CI ± 2.65). The seropositivity of ovine, caprine and bovine was found to be 71.7% (95% CI, 67.67% to 75.73%), 80.2% (95% CI, 75.79% to 84.61%) and 98.5% (95% CI, 96.43% to 100.57%), respectively. There was statistical significance in the seroprevalence of BTV among bovine, caprine and ovine (p dent from this study that blue tongue virus is endemic in Grenada.

关键词： Blue Tongue Virus c-ELISA Seroprevalence Grenada Ruminants

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Evaluation of Sensitivity and Positive Predictive Values of Cytopathologic Diagnosis of Solid Masses in Dogs

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Open Journal of Veterinary Medicine 2016年第6期6卷 95-98页

作者： Richard M. Kabuusu Tracy Volger Sachin Kumthekar Keshaw Tiwari Alfred Chikweto Muhammad I. Bhaiyat claude de allie Ruth Alexander Carla Richards Ravindra N. Sharma department of Pathobiology School of Veterinary Medicine St. George’s University St. George’s West Indies

In this retrospective study, a total of 275 solid masses were examined for cytopathologic diagnosis. Twenty four percent (67/275) of these cytologic samples were followed by surgical biopsy and histopathologic diagnosis, allowing for comparisons. On average, the cutaneous and subcutaneous solid masses were recognized when the dogs were aged between 6 and 9 years old. The origins of the solid masses included connective tissue tumors 37.1% (23/62), epithelial tissue tumors 33.9% (21/62), round cell tumors 19.4% (12/62), masses of inflammatory lesions 4.8% (3/62) and lesions due to other causes 4.8% (3/62). The sensitivity and positive predictive value (PPV) of cytopathology in the diagnosis of solid masses were 93% (62/67) and 97% (62/64), respectively. Generally, neo-plasms were over diagnosed by cytopathology as was indicated by the positive predictive value. Both the sensitivity and the PPV of cytopathology comparative to histopathology in the diagnosis of inflammatory processes were 100% (3/3). The inflammatory lesions were eventually confirmed as necrotizing myositis, necro-suppurative cystitis and endocrine inflammatory dermatopathy based on histopathology. Less than 8% (5/67) of samples were incorrectly diagnosed by cytology. The study showed high accuracy between cytological and histopathological examination of solid masses in dogs, and thus a reliable diagnostic tool in patient care.

关键词： Cytopathology Histopathology Dog Sensitivity Positive Predictive Value Solid Masses

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