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The.mal pre.e.e.ce. tole.ance.and te.pe.ature.de.e.de.t re.piration in the.California se. hare.<i>Aplysia californica</i>

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Agricultural Scie.ce. 2013年第6期4卷 46-52页

作者： Ana De.isse.Re.Fe.nando Díaz Alfre.o Salas-Garza Marco Gonzale. Victor Corde.o clara e. galindo-sanchez e.na sanchez-Castre.on Adolfo Sánche. Zamora Ale.e. Lice.-Navarro Instituto de.Inve.tigacione. Oce.nológicas Universidad Autónoma de Baja California Ensenada Baja California México Laboratorio de.Biología Ce.ular y Mole.ular Departamento de Biotecnología Marina Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE) Ensenada Baja California México Laboratorio de.e.ofisiología de.Organismos Acuáticos Departamento de Biotecnología Marina Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE) Ensenada Baja California México Laboratorio de.e.ofisiología de.Organismos Acuáticos Departamento de Biotecnología Marina Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE) Ensenada Baja California México Laboratorio de.Ge.ómica Funcional Departamento de Biotecnología Marina Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE) Ensenada Baja California México Unidad Multidisciplinaria de.Doce.cia e.Inve.tigación Facultad de Ciencias Universidad Autónoma de México Sisal México

The.the.more.ulatory be.avior of se. hare.Aplysia californica was de.e.mine. in a horizontal the.mal gradie.t;with a pre.e.re. te.pe.ature.(PT) of 18.3°C for the.day cycle.and 20.8°C for the.night cycle. The.displace.e.t ve.ocity de.onstrate. an initial rate.of 30 cm·hˉ1 and gradually the.ve.ocity diminishe. to 18 cm·hˉ1 with se.e.al fluctuations mainly at 02:00 am. Critical Te.pe.ature.Maxima (CTMax re.e.s to the.te.pe.ature.point whe.e.at le.st 50% of the.e.pe.ime.tal group have.a loss of attachme.t) was me.sure. at thre. acclimation te.pe.ature. (16°C, 19°C and 22°C). At the.lowe.t acclimation te.pe.ature.(16°C), 50% of the.e.pe.ime.tal group had an attachme.t loss at CTMax 32.7°C, and in a highe. acclimation te.pe.ature.(22°C) CTMax was 36.2°C. The.Oxyge. Consumption Rate.(OCR) was close.y corre.ate. to acclimation te.pe.ature. and at 16°C and 19°C se. hare.had a re.ative.y stable.me.abolic rate. with OCR incre.sing to 9 mg O2 hˉ1·kgˉ1 w.w. in a highe. acclimation te.pe.ature.

关键词： The.more.ulatory Be.avior CTMax Oxyge. Consumption Aplysia californica

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Re.re.sion of the.Auxin Re.ponse.Pathway Incre.se. Arabidopsis Susce.tibility to Ne.rotrophic Fungi

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Mole.ular Plant 2008年第3期1卷 496-509页

作者： Francisco Llore.te.Paul M usksktt Andre. sanchez-Valle. Ge.ma Lope. Brisa Ramos clara sanchez-Rodrigue. Lucia Jorda Jane.Parke. Antonio Molina Ce.tro de.Biote.nologia y Ge.bmica de.Plantas (CBGP) Departamento de Biotecnologia-UPM ETS Ingenieros Agrbnomos Avda Complutense E-28040Madrid Spain De.artme.t of Plant-Microbe.Inte.actions Max Planck Institute for Plant Breeding Research Carl-von-Linne Weg 10 50829 Cologne Germany

In plants, re.istance.to ne.rotrophic pathoge.s de.e.ds on the.inte.play be.we.n diffe.e.t hormone.syste.s, such as those.re.ulate. by salicylic acid （SA）, jasmonic acid （JA）, e.hyle.e. and abscisic acid. Re.re.sion of auxin signaling by the.SA pathway was re.e.tly shown to contribute.to antibacte.ial re.istance. He.e. we.de.onstrate.that Arabidopsis auxin signaling mutants axrl, axr2, and axr6 that have.de.e.ts in the.auxin-stimulate. SCF （Skpl-Cullin- F-box） ubiquitination pathway e.hibit incre.se. susce.tibility to the.ne.rotrophic fungi Ple.tosphae.e.la cucume.ina and Botrytis cine.e.. Also, stabilization of the.auxin transcriptional re.re.sor AXR3 that is normally targe.e. for re.oval by the.SCF-ubiquitin/prote.some.machine.y occurs upon P. cucume.ina infe.tion. Pharmacological inhibition of auxin transport or prote.some.function e.ch compromise.ne.rotroph re.istance.of wild-type.plants to a similar e.te.t as in non-tre.te. auxin re.ponse.mutants. The.e.re.ults sugge.t that auxin signaling is important for re.istance.to the.ne.rotro- phic fungi P. cucume.ina and B. cine.e.. SGTlb （one.of two Arabidopsis SGT1 ge.e. e.coding HSP90/HSC70 co-chape.one.） promote. the.functions of SCF e.-ubiquitin ligase.comple.e. in auxin and JA re.ponse. and re.istance.conditione. by ce.tain Re.istance.（R） ge.e. to biotrophic pathoge.s. We.find that sgtlb mutants are.as re.istant to P. cucume.ina as wild-type.plants. Conve.se.y, auxin/SCF signaling mutants are.uncompromise. in RPP4-trigge.e. re.istance.to the.obligate.biotrophic oomyce.e. Hyalope.onospora parasitica. Thus, the.pre.ominant action of SGTlb in R ge.e.conditione. re.is- tance.to oomyce.e. appe.rs to be.at a site.othe. than assisting SCF e.-ubiquitin ligase.. Howe.e., ge.e.ic additivity of sgtlb axrl double.mutants in susce.tibility to H. parasitica sugge.ts that SCF-me.iate. ubiquitination contribute. to lim- iting biotrophic pathoge. colonization once.plant-pathoge. compatibility is e.tablishe..

关键词： plant de.e.se innate.immunity ne.rotrophic fungi auxin signaling prote.some.

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Astrocyte. and re.rograde.de.e.e.ation of nigrostriatal dopamine.gic ne.rons in Parkinson’s dise.se.re.oving axonal de.ris

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Translational Ne.rode.e.e.ation 2021年第4期10卷 554-572页

作者： Ingrid Morale. Ricardo Pue.tas-Ave.daño Albe.to sanchez Adrian Pe.e.-Barre.o clara Rodrigue.-Sabate.Manue. Rodrigue. Laboratory of Ne.robiology and e.pe.ime.tal Ne.rology Department of Basic Medical SciencesFaculty of MedicineLa Laguna UniversityLa LagunaTenerifeCanary IslandsSpain Ce.te. for Ne.worke. Biome.ical Re.e.rch on Ne.rode.e.e.ative.Dise.se.(CIBe.Ne.) MadridSpain

Obje.tive.The.dopamine.gic nigrostriatal ne.rons(DA ce.ls)in he.lthy pe.ple.pre.e.t a slow de.e.e.ation with aging,which produce. ce.lular de.ris throughout *** 2%-5%of pe.ple.pre.e.t rapid ce.l de.e.e.ation of more.than 50%of DA ce.ls,which produce. Parkinson's dise.se.PD).Ne.roinflammation acce.e.ate. the.ce.l de.e.e.ation and may be.critical for the.transition be.we.n the.slow physiological and the.rapid pathological de.e.e.ation of DA ce.ls,particularly whe. it activate. microglial ce.ls of the.me.ial fore.rain bundle.ne.r dopamine.gic *** synaptic de.ris produce. by DA ce.l de.e.e.ation may trigge. the.parkinsonian ne.roinflammation,this study inve.tigate. the.re.oval of axonal de.ris produce. by re.rograde.de.e.e.ation of DA ce.ls,paying particular atte.tion to the.re.ative.role. of astrocyte. and ***:Rats and mice.we.e.inje.te. in the.late.al ve.tricle. with 6-hydroxydopamine.inducing a de.e.e.ation of dopamine.gic synapse. in the.striatum which was not accompanie. by non-se.e.tive.tissue.damage.microgliosis or *** possible.re.rograde.de.e.e.ation of dopamine.gic axons,and the.production and me.abolization of DA-ce.l de.ris we.e.studie. with immunohistoche.ical me.hods and analyze. in confocal and e.e.tron microscopy ***:The.se.e.tive.de.e.e.ation of dopamine.gic synapse. in the.striatum was followe. by a re.rograde.de.e.e.ation of dopamine.gic axons whose.de.ris was found within sphe.oids of the.me.ial fore.rain *** sphe.oids re.aine. mitochondria and most(e.g.,tyrosine.hydroxylase.the.dopamine.transporte. prote.n,and amyloid pre.ursor prote.n)but not all(e.g.,α-synucle.n)prote.ns of the.de.e.e.ating dopamine.gic *** showe. initial(autophagosome.)but not late.lysosome.)compone.ts of autophagy(incomple.e.autophagy).The.e.sphe.oids we.e.pe.e.rate. by astrocytic proce.se. of the.me.ial fore.rain bundle.which provide. the.lysosome. ne.de. to continue.the.de.radation of dopamine.gic de.ri

关键词： Astrocyte Phagocytosis Parkinson's dise.se Dying-back de.e.e.ation Me.ial fore.rain bundle Sphe.oid

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Arabidopsis He.e.otrime.ic G-prote.n Re.ulate. Ce.l Wall De.e.se.and Re.istance.to Ne.rotrophic Fungi

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Mole.ular Plant 2012年第1期5卷 98-114页

作者： Magdale.a De.gado-Ce.e.o clara sanchez-Rodrigue. Viviana e.cude.o e.a Mie.e. Paula Virginia Fe.nande. Lucia Jorda Camilo He.nande.-Blanco Andre. sanchez-Valle. Pawe. Be.nare. Paul Schulze.Le.e.t Shauna Some.ville. Jose.Manue. e.te.e. Staffan Pe.sson Antonio Molina Ce.tro de.Biote.nologia y Ge.omica de.Plantas （UPM-INIA） Universidad Politecnica de Madrid Campus de Montegancedo E-28223-Pozuelo de Alarcbn （Madrid） Spain Max-Planck-lnstitute.fLir Mole.ulare.Pflanze.physiologie Am MQhlenberg 2 14476 Potsdam Germany Laboratorio de.Fisiologia y Biologia Mole.ular IFIByNE （CONICET） FCEyN-Universidad de Buenos Aires Pab. II Intendente Guiraldes 2160 Buenos Aires C1428EGA Argentina Pre.e.t addre.s： Laboratory of Phytopathology Wageningen University Droevendaalsesteeg 1 6708 PB Wageningen The Netherlands Max Planck Institut for Zuchtungsforschung Department of Plant Microbe Interactions Carl-von-Linne-Weg 10 D-50829 Koln Germany Institute.of Bioorganic Che.istry Polish Academy of Sciences Noskowskiego 12/14 61-704 Poznan Poland e.e.gy Bioscie.ce. Institute 130 Calvin Hall University of California at Berkeley Berkeley CA 94720 USA

The.Arabidopsis he.e.otrime.ic G-prote.n controls de.e.se.re.ponse. to ne.rotrophic and vascular fungi. The.agbl mutant impaire. in the.Gβ subunit displays e.hance. susce.tibility to the.e.pathoge.s. Gβ/AGB1 forms an obligate.dime. with e.the. one.of the.Arabidopsis Gγsubunits （γ1/AGG1 and γ2/AGG2）. Accordingly, we.now de.onstrate.that the.aggl agg2 double.mutant is as susce.tible.as agbl plants to the.ne.rotrophic fungus Ple.tosphae.e.la cucume.ina. To e.ucidate.the.mole.ular basis of he.e.otrime.ic G-prote.n-me.iate. re.istance. we.pe.forme. a comparative.transcriptomic analysis of agbl-1 mutant and wild-type.plants upon inoculation with P cucume.ina. This analysis, toge.he. with me.ab- olomic studie., de.onstrate. that G-prote.n-me.iate. re.istance.was inde.e.de.t of de.e.sive.pathways re.uire. for re.istance.to ne.rotrophic fungi, such as the.salicylic acid, jasmonic acid, e.hyle.e. abscisic acid, and tryptophan-de.ive. me.abolite. signaling, as the.e.pathways we.e.not impaire. in agbl and aggl agg2 mutants. Notably, many mis-re.uiate. ge.e. in agbl plants we.e.re.ate. with ce.l wall functions, which was also the.case.in aggl agg2 mutant. Bioche.ical analyse. and Fourie. Transform InfraRe. （FTIR） spe.troscopy of ce.l walls from G-prote.n mutants re.e.le. that the.xylose.conte.t was lowe. in agbl and aggl agg2 mutants than in wild-type.plants, and that mutant walls had similar FTIR spe.-tratype., which diffe.e. from that of wild-type.plants. The.data pre.e.te. he.e.sugge.t a canonical functionality of the.Gβ and Gγ1/γ2 subunits in the.control of Arabidopsis immune.re.ponse. and the.re.ulation of ce.l wall composition.

关键词： Plant immunity ce.l wall indole.glucosinolate. xylose ne.rotropic fungi G-prote.n.

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