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Development of Standard Reagents for Avian Influenza Virus Subtypes Diagnosis

Journal of Agricultural Science and Technology(A) 2019年第1期9卷 26-37页

作者： Maria Angela Orsi Soraya Cecilia Albieri Camillo Eluana Carolina Fortunato Christian Steffe Domingues Dilmara Reischak Margarida Maria Hoppner Zaroni Tania Rosária pereira Freitas Terra A.Jenson Janice C.Pedersen Agriculture and Livestock National Laboratory-Sao Paulo LANAGRO-SPMinistry of AgricultureLivestock and SupplayMAPACampinasSao PauloBrazil Agriculture and Livestock National Laboratory-Minas Gerais LANAGRO-MGMinistry of AgricultureLivestock and SupplayMAPAPedro LeopoldoMinas GeraisBrazil U.S.Department of Agriculture Animal and Plant Health Inspection ServiceVeterinary ServicesNational Veterinary Services Laboratories(NVSL)AmesIAUSA

Avian influenza(AI)is one of the most relevant viruses in the poultry *** AI virus(AIV)transmission from birds to human causing severe cases and mortality enhanced the magnitude of AI for public ***,the AIV diagnosis laboratories should be able to detect and identify endemic,epidemic and seasonal influenza strains and other wildlife influenza subtypes that cross the country’s *** development in quality controls in according with international rules comes to improve the performance of *** this purpose,the Brazilian Reference Laboratory for Avian Influenza(LANAGRO-SP)established a cooperation with the World Organization for Animal Health(OIE)to produce AIV master seeds,inactivated antigens and antiserum to attend the necessities of Brazil and other South America countries under the high quality control for all *** of AIV master seed lots and seventeen of inactivated antigens lots produced reached hemagglutination(HA)titers of 1:512 and 1:256,*** addition,fifteen AIV antiserum lots with hemagglutination inhibition(HI)titers reaching 1:4,096 were *** AIV reference reagents produced and applied in laboratory routine successfully.

关键词： Avian influenza virus reference reagents subtypes quality control Brazil

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CFD predictions for hazardous area classification

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中国化学工程学报（英文版） 2019年第1期

作者： Andrey Oliveira de Souza Aurélio Moreira Luiz Ant?nio Tavernard pereira Neto Ant?nio Carlos Brandao de Araujo Heleno Bispo da Silva Sidinei Kebler da Silva Jose Jailson Nicacio Alves

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广东农村信息服务项目实施效果分析——以“山村信息化”和“信息直通车”为例

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南方农村 2013年第1期 4-9页

作者：易法敏 CAROL Ting 华南农业大学经济管理学院澳门大学资讯管理系

我国的农村信息化目前处于整合资源、统一平台、强化服务阶段。广东的农村信息化起步早,分析广东的农村信息服务项目实施效果,有助于提炼出很多普遍性的问题。本文认为,政府有必要对前期的农村信息服务项目建设效果进行评估,并在今后的... 详细信息

我国的农村信息化目前处于整合资源、统一平台、强化服务阶段。广东的农村信息化起步早,分析广东的农村信息服务项目实施效果,有助于提炼出很多普遍性的问题。本文认为,政府有必要对前期的农村信息服务项目建设效果进行评估,并在今后的工作中加强部门间协调和引导社会各方力量间的合作。首先,在大规模的网络建设之前应该对政策措施实施更好的评估;另外,必须协调不同各部门间的无序竞争,并以法定形式保证部门行动的一致性和政策的连续性。

关键词：农村信息服务山区信息化信息直通车

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Health（健康） 2020年第10期

作者： Lucas Lima de Carvalho Lucas Rodrigues claro Amanda dos Santos Cabral Marcela pereira da Silva Mello Bruna Liane Passos Lucas Marcelly Valadares Souza Simone Fonseca Lucas Alexandre Oliveira Tellles Ravini Fernandez dos Santos Vieira dos Santos Marcia Augusta pereira dos Santos claudia Lima Campos Alzuguir Maria Cristina Dias da Silva Antonio Eduardo Vieira dos Santos Maria Antonieta Rubio Tyrrell Eduardo Alexander Júlio César Fonseca Lucas

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Murine model to study brain,behavior and immunity during hepatic encephalopathy

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World Journal of Hepatology 2014年第4期6卷 243-250页

作者： Lindisley Ferreira Gomides Pedro Elias Marques Bruno Engler Faleiros Rafaela Vaz pereira Sylvia Stella Amaral Thais Reis Lage Gustavo Henrique Souza Resende Patricia Alves Maia Guidine Giselle Foureaux Fabíola Mara Ribeiro Fabiana Paiva Martins Marco Antonio Peliky Fontes Anderson José Ferreira Remo Castro Russo Mauro Martins Teixeira Márcio Flávio Moraes Antonio Lúcio Teixeira Gustavo Batista Menezes Laboratório de Imunobiofotonica Department of MorphologyICBUFMGBelo Horizonte 31270-901Brazil School of Medicine UFMGBelo Horizonte 31270-901Brazil Department of Physiology ICBUFMGBelo Horizonte 31270-901Brazil Department of Morphology UFMGBelo Horizonte 31270-901Brazil Department of Biochemistry and Immunology UFMGBelo Horizonte 31270-901Brazil

AIM:To propose an alternative model of hepatic encephalopathy(HE) in mice,resembling the human features of the ***:Mice received two consecutive intraperitoneal injections of thioacetamide(TAA) at low dosage(300 mg/kg).Liver injury was assessed by serum transaminase levels(ALT) and liver histology(hematoxylin and eosin).Neutrophil infiltration was estimated by confocal liver intravital *** was evaluated using prolonged prothrombin and partial thromboplastin *** parameters were measured through tail *** levels were quantified in serum and brain ***(EEG) and psychomotor activity score were performed to show brain *** edema was evaluated using magnetic resonance ***:Mice submitted to the TAA regime developed massive liver injury,as shown by elevation of serum ALT levels and a high degree of liver *** intense hepatic neutrophil accumulation occurred in response to TAA-induced liver *** led to mice mortality and weight loss,which was associated with severe ***,TAA-treated mice presented with increased serum and cerebral levels of ammonia,in parallel with alterations in EEG spectrum and discrete brain edema,as shown by magnetic resonance *** agreement with this,neuropsychomotor abnormalities ensued 36 h after TAA,fulfilling several HE features observed in *** this context of liver injury and neurological dysfunction,we observed lung inflammation and alterations in blood pressure and heart rate that were indicative of multiple organ dysfunction ***:In summary,we describe a new murine model of hepatic encephalopathy comprising multiple features of the disease in humans,which may provide new insights for treatment.

关键词： Hepatic encephalopathy Liver injury Thioacetamide Neurological dysfunction Neuropsychomotor abnormalities Intracranial hypertension Cerebral herniation

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囊性纤维化肝病肝纤维化的血清标志物

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世界核心医学期刊文摘（胃肠病学分册） 2005年第3期1卷 57-57页

作者： pereira T.N. Lewindon P.J. Smith J.L. 姜志茹

Hepatic fibrosis contributes to adverse outcome in cystic fibrosis (CF). Early detection of CF liver disease (CFLD) may identify patients at risk of significant complications. To evaluate the utility of serum markers to detect hepatic fibrosis in children with CFLD vs. CF patients without liver disease (CFnoLD) and controls. Sera from 36 CFLD, 30 CFnoLD and 39 controls were assessed for tissue inhibitor of matrix metalloproteinase (MMP) (TIMP)-1, collagen (cl) IV, MMP-2, hyaluronic acid (HA) and prolyl hydroxylase (PH) by enzyme immunoassay and were correlated with hepatic fibrosis score in CFLD. TIMP-1, PH and cl IV were increased in CFLD vs. CFnoLD and controls. Fibrosis score was negatively correlated with TIMP 1 (r=-0.34, P=0.06) and PH(r=-0.48, P=0.008). Receiver operating characteristics analysis showed cl IV (AUC 0.785, Pcl IV, TIMP-1, PH to identify CFLD (AUC 0.831, Pcl IV, PH may be indicators of hepatic fibrogenesis in CF. Increased TIMP-1, PH may be early markers of CFLD.

关键词：肝纤维化血清标志物囊性纤维化 TIMP 酶联免疫法脯氨酰羟化酶早期标志早期检测负相关能将

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颈动脉和椎动脉经管腔血管成形术研究中颈动脉血管成形术、支架术和内膜剥脱术后再狭窄

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世界核心医学期刊文摘（神经病学分册） 2005年第7期1卷 56-56页

作者： McCabe D.J.H. pereira A.C. clifton A. M.M.Brown 雷翀 Box 6 Natl. Hosp. for Neurol./Neurosurg. Queen Square London WC1N 3BG United Kingdom Prof.

Background and Purpose:Carotid and Vertebral Artery Transluminal Angioplasty Study (CAVATAS) patients with carotid stenosis were randomized between endovascu lar treatment and endarterectomy. The rates of residual severe stenosis and rest enosis and their contribution to recurrent symptoms was ***:Endovas cular patients:were treated by balloon angioplasty alone (88%) or stenting (22 %). Patches were used in 63%of endarterectomy patients. Carotid stenosis was c ategorizedas mild (0%to 49%), moderate (50%to 69%), severe (70%to 99%), or occluded, using standardized Doppler ultrasound criteria at the examination clo sest to 1 month (n=283) and 1 year (n=347) after treatment. Recurrent cerebrovas cular symptoms during follow-up were analyzed. Results:More patients had ≥70 %stenosis of the ipsilateral carotid artery 1 year after endovascular treatment than after endarterectomy (18.5%versus 5.2%, P=0.0001). Residual severe steno sis was present in 6.5%of patients at 1 month after endovascular *** een 1 month and 1 year, restenosis to ≥70%stenosis occurred in 10.5%of the en dovascular group. After endarterectomy,1.7%had residual severe stenosis at 1 mo nth, and 2.5%developed severe restenosis. The results were significantly better after stenting compared with angioplasty alone at 1 month(Pclusions:Carotid stenos is 1 year after endovascular treatment is partly explained by poor initial anato mical results and partly by restenosis. The majority of patients were treated by angioplasty without stenting. Further randomized studies are required to determ ine whether new

关键词：血管成形术内膜剥脱术再狭窄支架术血管内治疗 endarterectomy stenosis endovascular carotid 动脉内膜切除术

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Antimicrobial Activity of a Formulation of Libidibia ferrea L. against Microorganisms of the Dental Biofilm

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Advances in Microbiology（微生物学） 2020年第9期

作者： Raquel de Oliveira Marreiro Maria Fulgência Costa Lima Bandeira Carina Toda Fábio Correia Sampaio Tatiane pereira de Souza Gisely Naura Venâncio Wanderleia Monteiro de Souza Nikeila Chacon de Oliveira Conde

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用腐植酸的结构-性质-功能关系来解释植物生物活性

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腐植酸 2018年第6期 61-61页

作者： Andrés Calderín García Luiz Gilberto Ambrosio de Souza Marcos Gervasio pereira 周丽平中国农业科学院农业资源与农业区划研究所

尽管明确腐殖质(HSs)的结构一性质.功能之间的关系的相关研究已经取得了进展,但仍未有定论。我们分析了37个HSs组分的同位素组成、结构特征和刺激植物根系生长的特性。结果发现:虽然碳的来源不同(C3或C4),但土壤中提取的HSs和腐植酸(HAs... 详细信息

尽管明确腐殖质(HSs)的结构一性质.功能之间的关系的相关研究已经取得了进展,但仍未有定论。我们分析了37个HSs组分的同位素组成、结构特征和刺激植物根系生长的特性。结果发现:虽然碳的来源不同(C3或C4),但土壤中提取的HSs和腐植酸(HAs)在结构上是相似的。

关键词：腐植酸结构生物活性植物性质同位素组成根系生长 HSs

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血管紧张素转换酶基因缺失多态性对心力衰竭患者症状发生和生存率的调节

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世界核心医学期刊文摘（心脏病学分册） 2005年第7期1卷 46-46页

作者： Cuoco M.A.R. pereira A.C. De Freitas H.F.G. 高登峰 Heart Institute(InCor) Clinical Division Univ. of S. Paulo Medical School Av. Dr. Eneas Carvalho Aguiar 44 So Paulo 05403-000 Brazil

Background: Angiotensin-converting enzyme is involved in the pathophysiology of heart failure. We hypothesized that clinical characteristics as well as survival rate in patients with heart failure of different etiologies may be modulated by functional variants DD, ID and II of the angiotensin-converting enzyme gene. Methods: We studied 333 patients with heart failure, aged 43.3±10.5 years, 262(78.7%) men and 71(21.3%) women. Heart failure was ascribed to idiopathic dilated cardiomyopathy in 125 patients. Heart failure was caused by ischemic heart disease in 63 patients, Chagas’disease in 58, hypertensive heart disease in 41, alcoholic cardiomyopathy in 24, and was due to other etiologies in 22 patients. Statistical analysis was performed with the χ2 test, Student’s t-test, analysis of variance, Kaplan-Meier and Cox proportional hazards methods. Results: The DD genotype was associated with increased systolic left ventricular diameter(p=0.031). Earlier onset of symptoms was observed in patients with alcoholic cardiomyopathy and DD genotype(p=0.033, codominant D) and in patients with hypertensive cardiomyopathy and DD genotype(p=0.048, codominant D; p=0.024, recessive D). Mortality was higher in patients older than 50 years with DD genotype(p=0.007, codominant D; p=0.002, recessive D). Variables independently associated with higher mortality in patients older than 50 years were age, diabetes mellitus, Chagas’disease etiology and DD genotype. Conclusions: These results add evidence for an association of the DD genotype of the angiotensin-converting enzyme gene with earlier onset of symptoms and decreased survival rate of selected patients with heart failure.

关键词：基因缺失酒精性心肌病多态性缺血性心脏病病理生理学显性基因左心室隐性基因试验研究统计学分析

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