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Challenges for the Characterization of Genetically Modified Animals by the qPCR Technique in the Era of Genomic Editing

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Veterinary Science Research 2021年第1期3卷 1-11页

作者： Ribrio Ivan Tavares pereira Batista Darcio Italo Alves Teixeira Vicente Jose de Figueiredo Freitas Luciana Magalhaes Melo Joanna Maria Goncalves Souza-Fabjan Laboratorio de Fisiologia e Controle da Reproducao Faculdade de VeterinariaUniversidade Estadual do Ceara(UECE)Av.Dr.Silas Munguba 1700FortalezaCE60740-903Brazil Faculdade de Veterinaria Universidade Federal Fluminense(UFF)Rua Vital Brazil Filho64NiteroiRJ24230-340Brazil Molecular Genetics Research Unit University Center Fametro(Unifametro)Rua Conselheiro Estelita500FortalezaCE60010-260Brazil

Characterization of genetically modified organisms through determination of zygosity and transgene integration concerning both copy number and genome site is important for breeding a transgenic line and the use of these organisms in the purpose for which it was *** blot,fluorescence in situ hybridization or mating are demanding and time-consuming techniques traditionally used in the characterization of transgenic organisms and,with the exception of mating,give ambiguous *** the emergence of the real-time quantitative PCR technology,different applications have been described for the analysis of transgenic organisms by determination of several parameters to transgenic ***,the accuracy in quantitation by this method can be influenced in all steps of *** review focuses on the aspects that influence pre-analytical steps(DNA extraction and DNA quantification methods),quantification strategies and data analysis in quantification of copy number and zygosity in transgenic animals.

关键词： Absolute quantification Copy number DNA extraction Relative quantification Zygosity

Psychological Complaints at Psychological Emergency Service Associated with Referral to Extended Screening in a Psychology School clinic

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Journal of Psychological Research 2022年第1期4卷 10-17页

作者： Caíque Rossi Baldassarini Naiara Alves pereira Larissa Nicolau Pitta Marcelo Monteiro de Souza Caroline de Oliveira Zago Rosa Fernanda Pessolo Rocha Department of Psychology Barão de MauáUniversity Center14090-062Brazil

The Psychological Emergency Service(PES)at psychology school clinics is an unscheduled and free psychological service to meet urgent *** this service,some patients whose complaints require more time for clinical work are referred to the Extended Screening(ES),a modality composed of six extra *** study aims to analyze the sociodemographic profile of patients seen on PES in a Brazilian Psychology School clinic,and to identify the demands that motivated referral to the ES,for better qualification of the care *** is a descriptive analysis research,carried out based on data from the medical records of 46 patients who went through the PES and were referred to the ES at the institution,between the years 2019 and *** data were collected and,from the session reports,a content analysis of the thematic analysis modality was performed to identify the *** participants’age ranged from 18 to 65 years,with a mean of 32.28 years(sd=10.95).There was a predominance of female participants(71.74%),that completed High School(39.14%)and had an income from one to two Brazilian minimum wages(32.61%).The most frequent complaints were depressive symptoms(56.52%)and difficulties in interpersonal relationships(32.61%).The results obtained,besides allowing the survey of the social and demographic profile of the clientele of the PES at the Psychology School clinic,and demonstrating its social relevance by providing free psychological care,also show to be of great importance for the definition of more accurate criteria for referral to the ES of patients seen on PES.

关键词： Psychological emergency service Extended screening Psychology school clinic

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子宫加压缝合治疗子宫松弛继发产后出血

世界核心医学期刊文摘（妇产科学分册） 2006年第2期2卷 6-6页

作者： pereira A. Nunes F. Pedroso S. 刘亦恒 Dr.Servic.o de Ginecologia e Obstetrcia Hospital Garcia de Orta Pragal-2800 Almada Portugal

Objective: To demonstrate the usefulness of a new method of applying compressive sutures to treat postpartum bleeding secondary to uterine atony. Methods: Multiple sutures were applied longitudinally and transversally around the uterus of 7 women with postpartum uterine atony and postpartum bleeding. Results: The procedure was successful in all cases. Conclusion: Compressive sutures of the uterus were effective in treating uterine atony with postpartum ***:Effectiveness of treatment strategies of some women with pelvic inflammatory disease: A randomized trial Ness R.B./Trautmann G./Richter H.E. et al. [Dr. R.B. Ness, University of Pittsburgh, Graduate School of Public Health, A530 Crabtree Hall, 130 DeSoto Street, Pittsburgh, PA 15261, United States]Abstract:Objective: Among all women with pelvic inflammatory disease (PID), prevention of adverse reproductive consequences appears to be similarly achieved by outpatient treatment and inpatient treatment. We assessed whether outpatient is as effective as inpatient treatment in relevant age, race, and clinical subgroups of women with PID. Methods: Women with clinical signs and symptoms of mild-to-moderate pelvic inflammatory disease (n = 831) were randomized into a multicenter trial of inpatient treatment, initially employing intravenous cefoxitin and doxycycline compared with outpatient treatment consisting of a single intramuscular injection of cefoxitin and oral doxycycline. Comparisons between treatment groups during a mean of 84 months of follow-up were made for pregnancies, live births, time to pregnancy, infertility, PID recurrence, chronic pelvic pain, and ectopic pregnancy. Results: Outpatient treatment assignment did not adversely impact the proportion of women having one or more pregnancies, live births, or ectopic pregnancies during follow-up; time to pregnancy; infertility; PID recurrence; or chronic pelvic pain among women of various races; with or without previous PID; with or without baselin

关键词：子宫松弛产后出血

Morphology Characterization and Kinetics Evaluation of Pitting Corrosion of Commercially Pure Aluminium by Digital Image Analysis

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社会经济地位,而不是非洲血统的基因组,与血压相关:Bambui-Epigen(巴西)老龄化队列研究

Materials Sciences and Applications（材料科学与应用期刊） 2012年第5期

作者： Marinalda C. pereira José W. J. Silva Heloisa A. Acciari Eduardo N. Codaro Luis R. O. Hein

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中华高血压杂志 2016年第2期24卷 109-109页

作者：刘莉叶鹏 Lima-Costa MF Mello Mambrini JV Leite ML Peixoto SV Firmo JO Loyola Filho AI Gouveia MH Leal TP pereira AC Macinko J Tarazona-Santos E

该研究旨在确定非洲基因组起源对巴西以社区为基础的多种族老年人群基线和11年血压变化的作用。方法：纳入随访11年的Bambui-Epigen（巴西）老龄化队列研究中的≥60岁受试者1272人的资料,观察指标为收缩压、舒张压和高血压控制率。潜在... 详细信息

该研究旨在确定非洲基因组起源对巴西以社区为基础的多种族老年人群基线和11年血压变化的作用。方法：纳入随访11年的Bambui-Epigen（巴西）老龄化队列研究中的≥60岁受试者1272人的资料,观察指标为收缩压、舒张压和高血压控制率。潜在的混杂因素包括人口学特征、社会经济地位（教育和家庭收入）、健康指标（吸烟、久坐的生活方式、高密度脂蛋白胆固醇、腰围、糖尿病和心血管病等）、抗高血压药物的使用。

关键词：社会经济地位血压变化队列研究基因组老龄化巴西非洲高密度脂蛋白胆固醇

Economic Analysis of the Use of Restrictive Food Management in the Cultivation of Tilapia(Oreochromis niloticus)in a Recirculation System

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Journal of Agricultural Science and Technology(B) 2022年第2期12卷 31-39页

作者： Giovanni Resende de Oliveira Thiago Archangelo Freato Juan Daniel Villacis Fajardo Tainára Cunha Gemaque Nancy Andrea Villacis Fajardo Daniel pereira da Costa Agricultural Research Company of Minas Gerais(EPAMIG) Experimental Field of LeopoldinaLeopoldinaMG 36700-000Brazil State University of Campinas(UNICAMP) Center for Meteorological and Climate Research Applied to AgricultureCampinasSP 13083-886Brazil University of Amapa State(UEAP) Campus Lakes TerritoryAmapáAP 68950-000Brazil Federal University of Minas Gerais(UFMG) Campus PampulhaBelo HorizonteMG 31270-010Brazil National University of the Peruvian Amazon(UNAP) Iquitos 16024Peru

The development of more adequate food programs regarding the intensity and duration of the restriction can be an effective way to minimize the effects of exaggerated food consumption and its negative consequences for the economy of the *** this sense,juvenile Nile tilapia(Oreochromis niloticus)(137±3 g)were submitted to different feeding strategies in a recirculation *** feeding strategies were tested in subsequent weekly cycles:continuous feeding;skipping one meal a week;suppression of two non-consecutive meals a week;suppression of three non-consecutive meals a week;suppression of four non-consecutive meals a *** performance,feed consumption in the post-restriction period and economic indicators were evaluated through projections and profitability *** intake occurred in all tested groups,so that the total feed intake(FI)did not differ significantly between them(p>0.05).Moderate food restriction provided a linear increase in the relative gross margin for each treatment with each change in salary level,as well as greater profitability when compared to the respective control group(p<0.05).

关键词： Feed management feed restriction carcass quality carcass yield operational yield economic efficiency

Non-Hodgkin’s Lymphoma Associated with Metastatic Lung Cancer: Case Report

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Advances in Lung Cancer 2021年第3期10卷 40-47页

作者： Miriam Viviane Baron Gabriela Di Lorenzo Garcia Scherer Célia Regina Martins Korzenieski Julia Braga da Silveira Carolina Boeira Soares Carina Marangoni Marcus Vinicius de Mello Pinto Aline Ronis Sampaio Alexandre Gomes Sancho Esteban Fortuny Pacheco Felice Picariello Joice Nedel Ott Isabel Cristina Reinheimer Carolina Gonçalves Pinheiro Nathália Ken pereira Iketani Cristine Brandenburg Danielle de Mello Florentino Patrícia Froes Meyer Bartira Ercília Pinheiro da Costa Postgraduate Program in Medicine and Health Sciences of the Pontifical Catholic University of Rio Grande do Sul (PUC/RS) Porto Alegre Brazil School of Medicine of the Pontifical Catholic University of Rio Grande do Sul (PUC/RS) Porto Alegre Brazil School of Health and Life Sciences of the Pontifical Catholic University of Rio Grande do Sul (PUC/RS) Porto Alegre Brazil Celulare Institute Rio de Janeiro Brazil Unigranrio Rio de Janeiro Brazil Universidad Finis Terrae Santiago Chile Università degli Studi di Napoli Federico II Napoli Italy Postgraduate Program in Health Sciences of the Faculty of Medicine of ABC (FMABC) S&#227 o Paulo Brazil Hospital Beneficiente Portuguesa of Belém Pará Brazil Federal University of Ceará (UFC) Fortaleza Brazil Uniredentor University Rio de Janeiro Brazil Centro Universitário do Rio Grande do Norte Natal RN Brazil

Lymphomas are neoplastic transformations that affect lymphoid cells. Diffuse large B-cell non-Hodgkin’s lymphoma has a high degree of cell proliferation, accounting for 30% of all lymphomas. Lung cancer is the leading cause of death worldwide and the recommended treatment is chemotherapy. Among the main complications resulting from non-Hodgkin’s lymphoma, lung cancer and chemotherapy used in their treatment, we can mention sepsis, acute kidney injury and febrile neutropenia. Febrile neutropenia can occur by suppressing the production of neutrophils. Sepsis, a widespread infection, is the main cause of acute kidney injury, which can also be caused by hydroelectrolytic complications or by nephrotoxicity. This is a report of a smoking patient with metastatic lung cancer who sought care due to progressive dysphagia, cough with chest pain, fever, and lower airways critical obstruction due to mediastinal lymphadenopathy, being diagnosed with diffuse large B-cell non-Hodgkin’s lymphoma. The patient evolved to death because of a significant worsening of the ventilatory pattern of multifactorial cause, mainly due to sepsis, acute kidney injury, and febrile neutropenia. The patient had mostly classic characteristics of her comorbidities, however, the overlapping of interrelated comorbidities led to the outcome of death. What is unusual about the present case report is that the patient’s characteristics, such as age, sex, and ethnicity, are opposite to those described as risk factors for diffuse large B-cell non-Hodgkin’s lymphoma.

关键词： Acute Kidney Injury Febrile Neutropenia Sepsis Chemotherapy

Early Hepatic Encephalopathy after a Transjugular Intrahepatic Portosystemic Shunt Procedure: Case Report

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Open Journal of Gastroenterology 2021年第6期11卷 81-88页

作者： Miriam Viviane Baron Gabriela Di Lorenzo Garcia Scherer Michele Paula dos Santos Andres Felipe Mantilla Santamaria Cristine Brandenburg Isabel Cristina Reinheimer Marcus Vinicius de Mello Pinto Aline Ronis Sampaio Alexandre Gomes Sancho Alexandre Gomes Sancho Esteban Fortuny Pacheco Felice Picariello Danielle de Mello Florentino Joice Nedel Ott Carolina Gonçalves Pinheiro Nathália Ken pereira Iketani Sonia Carvalho Patrícia Froes Meyer Bartira Ercília Pinheiro da Costa Postgraduate Program in Medicine and Health Sciences of the Pontifical Catholic University of Rio Grande do Sul (PUC/RS) Porto Alegre Rio Grande do Sul Brazil School of Medicine of the Pontifical Catholic University of Rio Grande do Sul (PUC/RS) Porto Alegre Rio Grande do Sul Brazil Industrial University of Santander (UIS) Bucaramanga Colombia University of Vale do Acaraú (IDJ/UVA) Ceará Brazil Celulare Institute Rio de Janeiro Brazil Unigranrio Rio de Janeiro Brazil Universidad Finis Terrae Santiago Chile Università degli Studi di Napoli Federico II Napoli Italy Uniredentor University Rio de Janeiro Brazil Postgraduate Program in Health Sciences of the Faculty of Medicine of ABC (FABC) S&#227o Paulo Brazil Hospital Beneficiente Portuguesa of Belém Pará Brazil Rigshospital Copenhagen East K&#248benhavn Denmark Centro Universitário do Rio Grande do Norte Natal Brazil

Patients with decompensated hepatic cirrhosis may present systemic alterations and dysfunction of multiple organs. Ascites, portal hypertension, esophageal varices, and hepatic encephalopathy are common complications arising from cirrhosis. The aim of this paper is to report a case of a patient with liver cirrhosis and the complications of a transjugular intrahepatic portosystemic shunt. Male, elderly, and ex-alcoholic, diagnosed with liver cirrhosis, ascites, and esophageal varices. He underwent transjugular intrahepatic portosystemic shunt due to portal hypertension and returned to the hospital after 24 hours with agitation and mental confusion. He had a bowel movement stop, neurological worsening, loss of renal function, hepatic hydrothorax, hepatic encephalopathy, hypernatremia, hypocalcemia, and hypophosphatemia. He underwent a new procedure to occlude the transjugular intrahepatic portosystemic shunt, showing improvement of the mental status and ascites. However, continued with decompensation and hydro-electrolytic disorders. He evolved with worsening of the ventilatory pattern, and neurological and renal function, with a fatal outcome. Esophageal varices due to portal hypertension can be corrected with the transjugular intrahepatic portosystemic shunt. However, complications such as hypernatremia, hepatic hydrothorax, and hepatic encephalopathy may occur. Therefore, there is a need for reintervention to shunt or reduce its caliber. Thus, for patients with advanced age and decompensated cirrhosis, the potential risks and benefits of this procedure should be carefully evaluated due to the risk of complications and death.

关键词： Ascites Hypernatremia Portasystemic Shunt Transjugular Intrahepatic Hepatic Encephalopathy

Crithidia deanei infection in normal and dexamethasone–immunosuppressed Balb/c mice

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