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Desmarestia anceps Montagne (Phaeophyceae) against Colorectal Cancer Cells: Cytotoxic Activity and Proapoptotic Effects

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Advances in Biological Chemistry 2022年第6期12卷 228-245页

作者： Rafaele Frassini Daniela Steffens Sidnei Moura Cesar Aguzzoli Aline Paternostro Martins Pio Colepicolo Mutue Toyota Fujii Nair S. Yokoya cláudio Martin pereira De pereira Ana cláudia Phillipus Miriam De Barcellos Falkenberg João Antonio Pêgas Henriques Mariana Roesch-Ely Laboratory of Applied Toxicology and Bioproducts University of Caxias do Sul RS Brazil Laboratory of Biosynthetic and Natural Products of Caxias do Sul RS Brazil Area of Exact Sciences and Engineering University of Caxias do Sul RS Brazil Laboratory of Biochemistry and Molecular Biology of Algae University of S&#227 o Paulo S&#227 o Paulo SP Brazil Nucleus Research Phycology Institute of Botany S&#227 o Paulo SP Brazil Laboratory of Lipidomics and Biorganic-LipidomicaBio Federal University of Pelotas Pelotas RS Brazil Study Group on Natural and Synthetic Products (GEPRONAS) Federal University of Santa Catarina SC Brazil

Impairment of apoptosis promotes abnormal cellular proliferation and accumulation of genetic alterations. Identifying bioactive compounds extracted from seaweeds that induce apoptosis in cancer cells may be explored as new agents for cancer chemoprevention and/or chemotherapy. The present study aimed to determine the chemical composition and biological activity of the chloroform crude extract and fractions from Antarctic seaweed Desmarestia anceps. The chloroform extract was obtained by three consecutive macerations and the fractionation by vacuum liquid chromatography. The chemical characterization of the extract and fractions was performed through Gas Chromatography. The cytotoxicity of the crude extract and fractions was evaluated by the MTT assay. Cell death and cell cycle evaluation after 24 hours of exposure to chloroform extract were performed by flow cytometry. A total of 48 compounds were identified. The results indicate that chloroform extract and its fractions presented cytotoxic activity against HCT 116 cell line in a dose dependent-manner. Proapoptotic events were observed after chloroform extract exposition, which promoted an increase of multinucleated cells and reduced cell viability. This study was the first to explore cytotoxic potential of seaweed D. anceps fractions against HCT colorectal cancer cell line, suggesting that these macroalgae may be a promising candidate against anticancer activity.

关键词： Antarctic Seaweeds Antitumor Activity Apoptosis Proapoptotic Effect Colorectal Cancer

Bioengineered exosomal-membrane-camouflaged abiotic nanocarriers: neurodegenerative diseases, tissue engineering and regenerative medicine

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Military Medical Research 2023年第5期10卷 699-725页

作者： Daniela Lopes Joana Lopes Miguel pereira-Silva Diana Peixoto Navid Rabiee Francisco Veiga Omid Moradi Zhan-Hu Guo Xiang-Dong Wang Joao Conde Pooyan Makvandi Ana claudia Paiva-Santos Department of Pharmaceutical Technology Faculty of Pharmacy of the University of CoimbraUniversity of Coimbra3000-548 CoimbraPortugal REQUIMTE/LAQV Group of Pharmaceutical TechnologyFaculty of Pharmacy of the University of CoimbraUniversity of Coimbra3000-548 CoimbraPortugal School of Engineering Macquarie UniversitySydneyNsW 2109Australia Centre for Molecular Medicine and Innovative Therapeutics Murdoch UniversityPerthWA 6150Australia Department of Chemistry Shahr-e-Qods BranchIslamic Azad UniversityTehran 374-37515Iran lntegrated Composites Laboratory(icl) Department of Mechanical and Construction EngineeringNorthumbria UniversityNewcastle Upon Tyne NE18STUK Department of Pulmonary and Critical Care Medicine Zhongshan HospitalFudan University Shanghai Medical CollegeShanghai 200032China Faculdade de Ciencias Medicas NOVA Medical SchoolUniversidade Nova de Lisboa1169-056 LisbonPortugal Centre for Toxicogenomics and Human Health GeneticsOncology and Human ToxicologyFaculdade de Ciencias MedicasNOVA Medical SchoolUniversidade Nova de Lisboa1169-056 LisbonPortugal school of Engineering Institute for Bioengineeringthe University of EdinburghEdinburgh EH93JLUK.

A bio-inspired strategy has recently been developed for camouflaging nanocarriers with biomembranes,such as natural cell membranes or subcellular structure-derived *** strategy endows cloaked nanomaterials with improved interfacial properties,superior cell targeting,immune evasion potential,and prolonged duration of systemic ***,we summarize recent advances in the production and application of exosomal membrane-coated *** structure,properties,and manner in which exosomes communicate with cells are first *** is followed by a discussion of the types of exosomes and their fabrication *** then discuss the applications of biomimetic exosomes and membrane-cloaked nanocarriers in tissue engineering,regenerative medicine,imaging,and the treatment of neurodegenerative ***,we appraise the current challenges associated with the clinical translation of biomimetic exosomal membrane-surface-engineered nanovehicles and evaluate the future of this technology.

关键词： Biomimetic Cell membrane coating Exosome Exosomal-membrane-coated nanoparticle Extracellular vesicle(EV)

Developing an integrated urban ecological efficiency framework for spatial ecological planning:A case on a tropical mega metropolitan area of the global south

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Geoscience Frontiers 2023年第1期14卷 298-309页

作者： Manob Das Arijit Das Paulo pereira Department of Geography University of Gour BangaMaldaWest BengalIndia Environmental Management Laboratory Mykolas Romeris University VilniusLithuania

Rapid urbanization has emerged as one of the most critical challenges to ecological sustainability in urban *** developing countries,the degradation of the ecosystem is more prominent due to the lack of urban ***,it has become urgent for researchers to identify the ecological efficiency(EE)changes imposed by urban expansion and promote sustainable land use *** study aims to develop a comprehensive urban ecological efficiency(UEE)framework in the Kolkata Metropolitan Area(KMA),India,from 2000 to *** component analysis(PCA)was used to develop a remote sensing-based UEE index(UEEI)based on five effective ecological parameters(Greenness,Dryness,Heat,Wetness and vegetation health.A single sensitivity parameter was also calculated to determine the role of a single parameter based on which management strategies can be carried *** findings showed that(i)there were substantial deteriorations of UEE in the last 20 *** 2000 the areas with good EE were about 65.5%which declined to 53.72%in 2010 and 20.87%in *** areas with good UEE decreased 68%and 61%from 2000 to 2020 and 2010 to 2020,respectively;(ii)the areas with good UEE were 52%in 2000,while 38%in *** urban centres(Bhadreshwar,Champdani,Srirampur,Bally,Howrah,Kamarhati,Baranagar,Dum Dum,South Dum Dum,Rajarhat,Bidhannagar)located around the Kolkata megacity are characterized by poor and very poor EE(ranges of 0.60-1.00).Thus,spatiotemporal pattern of UEE could assist to clarify the administrative responsibilities as well as *** addition to this,the UEE framework can help for scientific guidance of urban ecosystem protection and restoration through comprehensive spatial landscape planning.

关键词： Urban ecological efficiency Kolkata metropolitan area Ecosystem services Urban ecological management Urbanization

Effect of pre-and post-wildfire management practices on plant recovery after a wildfire in Northeast Iberian Peninsula

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Journal of Forestry Research 2020年第5期31卷 1647-1661页

作者： Marcos Francos Paulo pereira Xavier Ubeda GRAM(Grup de Recerca Ambiental Mediterrania) Department of GeographyUniversity of BarcelonaMontalegre608001 BarcelonaSpain Environmental Management Centre Mykolas Romeris UniversityVilniusLithuania

Fire and pre-or post-fire management practices shape the distribution and richness of plant ***,the effects of pre-and post-fire management on vegetation recovery were studied at different times,up to 18 months after a *** months after a 2015 wildfire,18 study plots were established(three 4-m^2 plots for each treatment),vegetation regrowth was monitored and vegetal species richness(S),evenness(IT),density(D),diversity(H0)and maximum diversity(HMax)after 2,10 and 18 *** treatments were(1)control,unaffected by 2015 wildfire;(2)no treatment(NT),burned in 2015 wildfire and not managed;(3)managed in 2005 and burned in 2015(M05B);(4)managed in 2015,2 months before wildfire(M15B);(5)cut and manual removal after the 2015 wildfire(CR);(6)cut and no trunk removal randomly deposited on topsoil after the 2015 wildfire(cl).All the treatments were carried out in a Pinus halepensis Miller *** 10 and 18 months after the wildfire,vegetation recovery was greater in NT,CR and cl plots than in M05B and M15B the *** 18 months after the wildfire,Brachypodium retusum(Pers.)*** Rosmarinus officinalis *** still dominant,especially in M15B,corroborating the belief that pre-fire treatment reduced ecosystem resilience and vegetal recovery compared to the NT and post-fire managed *** was significantly lower 10 months after wildfire in control plots,and IT was significantly higher in that inventory than previously in *** months after the wildfire,H0 was significantly lower in *** months post-wildfire,HMax was significantly lower in the control *** months after the wildfire,HMax,was significantly higher in CR,cl and M05B than in the control and M15B ***,pre-fire management was detrimental to post-fire vegetation recovery,while manual post-fire management proved beneficial.

关键词： Forest management Vegetation recovery Diversity Richness Evenness

Long-term forest management after wildfire(Catalonia,NE Iberian Peninsula)

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Journal of Forestry Research 2020年第1期31卷 269-278页

作者： Marcos Francos Xavier Ubeda Paulo pereira GRAM(Grup de Recerca Ambiental Mediterrania) Department of GeographyUniversity of BarcelonaMontalegre608001 BarcelonaSpain Environmental Management Centre Mykolas Romeris UniversityVilniusLithuania

Studies of post-fire soil status in Mediterranean ecosystems are common;however,few have examined the effects of long-term forest management after a wildfire on physicochemical soil ***,we analyzed differences in soil properties attributable to long-term postfire management and assessed the sustainability of these management practices in relation to the soil *** study area is located in Odena in the northeast region of the Iberian Peninsula consisted of the control forest(burned more than 30 years ago),low density forest(LD;burned in a wildfire in 1986 and managed in 2005)and high density forest(HD;burned in a wildfire in 1986 and no managed).For soils from each plot,we measured soil water repellency,aggregate stability,total nitrogen(TN),soil organic matter(SOM),inorganic carbon(IC),pH,electrical conductivity,extractable calcium,magnesium,sodium,potassium(K),phosphorus,aluminum(Al),manganese(Mn),iron(Fe),zinc,copper,boron,chrome,silicon and sulfur and calculated the ratios of C/N,Ca+Mg/(Na+K)^1/2,Ca/Al and Ca/*** differences were found in TN,IC,SOM,pH,K,Al,Mn,Fe and C/N ratio(p<0.05).All soil properties were found to have largely recovered their pre-fire *** were affected by the post-fire management practices implemented 20 years after the fire,as reflected in their respective physicochemical properties,so that soil properties at the control and LD sites are more similar today than those at the control and HD ***,sustainable forest management can overcome soil degradation in areas affected by wildfire in the medium-and long-term by improving soil properties.

关键词： Soil chemical properties Aggregate stability Post-fire management Wildfire risk Vegetal density

In Silico Pharmacokinetics Studies for Quinazolines Proposed as EGFR Inhibitors

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Open Journal of Medicinal Chemistry 2015年第4期5卷 106-115页

作者： Gabriela Souza Fernandes Michelle Bueno de Moura pereira Ana cláudia Barbosa Marinho Brisa Machado Ana Carla Moreira Matheus Puggina de Freitas Karen Luise Lang Joao Eustáquio Antunes Department of Medicine Federal University of Juiz de Fora Governador Valadares Brazil Department of Pharmacy Federal University of Juiz de Fora Governador Valadares Brazil Department of Chemistry Federal University of Lavras Lavras Brazil

In silico pharmacokinetics studies can aid the search for molecules with potential ability to be drug candidates. In this paper, a number of quinazoline candidates for epidermal growth factor receptor inhibitors—EGFR, important targets for the treatment of cancer, are computationally analyzed. The literature described that 69 quinazoline molecules were synthesized and the respective half maximum inhibitory concentrations (IC50) were obtained. A bilinear parabolic model was built to investigate the druglikeness by correlating the corresponding lipophilicities, which can be represented by the ideal Log P , with the optimal biological activity in terms of pIC50 values. Structural characteristics leading to improved pharmacokinetics parameters were then analyzed. Compound 56 exhibited the lowest IC50 and, therefore, it had the highest ability to inhibit the EGFR. In the present work, the most potent inhibitor 56 is not calculated to be the most promising drug candidate, since it’s out of the parabolic model obtained due to a Log P above 5, which is not within the expected optimum range. Finally, this work is an example of computational prediction that an experimentally, highly active EGFR inhibitor can be unsuccessful as drug candidate because of pitfalls in pharmacokinetics parameters.

关键词： Cancer Treatment Quinazoline Inhibitors Rational Drug Design Pharmacokinetics

Dehydrated Natural Feed for Cats: Influence on Apparent Digestibility Coefficient, Feces Scores, and Serum Immune, Antioxidant, and Metabolic Biomarkers

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Food and Nutrition Sciences 2024年第7期15卷 594-611页

作者： Bruno Giorgio de Oliveira Cécere Guilherme Luiz Deolindo Bianca F. Bissacotti Priscila Marquezin Copetti Maria Rosa Chitolina Schetinger Bruna Klein Matheus Delamea Baldissera Thiago pereira Ribeiro Walter Hugo Cuelho Suarez Daniel Pigatto Monteiro Aleksandro Schafer Da Silva Graduate Multicenter Program in Biochemistry and Molecular Biology University of the State of Santa Catarina Lages Brazil Department of Biochemistry and Molecular Biology Universidade Federal de Santa Maria (UFSM) Santa Maria Brazil Department of Animal Science Universidade Federal de Santa Maria (UFSM) Chapec Brazil Department of Health Science Universidade Franciscana (UFN) Santa Maria Brazil Department of Animal Nutrition Tectron-Tecnologia e Inovao Toledo Brazil

The present study aimed to determine whether natural dehydrated feed (DNF) can replace dry extruded feed while preserving the required daily intake, nutrient digestibility and benefits for immunity and antioxidant response in cats. The animals were divided into two groups of seven animals each. One group received DNF, and the other consumed commercial extruded dry feed (DCF). The experiment lasted 45 days and we measured daily consumption and collected blood and feces samples. The animals in the DNF group showed greater digestibility of fiber and mineral matter than DCF. Cats in the DNF group had a higher volume of feces. DNF cats had lower leukocyte and granulocyte counts and cholesterol levels. Serum enzymatic antioxidant responses and non-enzymatic were higher in DNF cats. Cats in the DNF group showed lower serum acute phase proteins in addition to a tendency toward a higher concentration of transferrin than in the DCF group. Fecal Escherichia coli and total coliform counts of cats fed DNF were higher than DCF. We conclude that the daily consumption of cats is adequate. This feed had greater digestibility of fiber and mineral matter, in addition to an improvement in antioxidant and immunological responses.

关键词： Alternative Feed Antioxidant Companion Animals Health

Antimicrobial and synergistic effects of lemongrass and geranium essential oils against Streptococcus mutans,Staphylococcus aureus,and Candida spp.

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World Journal of Critical Care Medicine 2024年第3期13卷 63-74页

作者： Patrícia Michelle Nagai de Lima Thaís Cristine pereira Lara Steffany de Carvalho Letícia Ferreira dos Santos Carlos Eduardo Rocha Oliveira Lucas de Paula Ramos Maria Cristina Marcucci Amjad Abu Hasna Luciane Dias de Oliveira Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000SPBrazil Anhembi Morumbi University School of MedicineSão Josédos Campos 12230-002SPBrazil Department of Restorative Dentistry Endodontics DivisionInstitute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000São PauloBrazil

BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral *** To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,***,and *** Minimum microbicidal concentration was determined following clinical and Laboratory Standards Institute *** synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide *** were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS *** and *** essential oils were as effective as 0.12%chlorhexidine against *** and *** monotypic biofilms after 24 *** 24 h *** essential oil at 0.25%was more effective than the nystatin group,and *** essential oil at 0.25%was as effective as the nystatin *** *** and *** essential oils are effective against ***,***,***,***,and *** at different concentrations after 5 min and 24 h.

关键词： Antifungal effect Lemongrass Geranium Candida albicans Candida dubliniensis Candida krusei Staphylococcus aureus Streptococcus mutans

GEM... The new IUGS Commission on Geoscience for Environmental Management

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Episodes 2005年第1期28卷 65-66页

作者： pereira, J Univ Kebangsaan Malaysia LESTARI Inst Environm & Dev Bangi 43600 Malaysia

At the International Geological Congress held in Florence, Italy in August 2004 the IUGS Council approved the formation of a new Commission on Geoscience for Environmental Management. This new Commission was formed to continue IUGS activities in the environment following the closure of the IUGS Commission on Geological Sciences for Environmental Planning(COGEOENVIRONMENT) which had completed its term (refer to separate article in this issue).

关键词： IUGS 地质学环境规划发展目标工作思路