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Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS

Asian Pacific Journal of Tropical Biomedicine 2015年第12期5卷 1046-1050页

作者： Erna Susanti ciptati Retty Ratnawati Aulanni'am Achmad Rudijanto Doctoral Program of Biomedical Science Faculty of Medicine Brawijaya University Department of Chemistry Faculty of Science Bandung Institute of Technology Biomedical Science Faculty of Medicine Brawijaya University

Objective:To identify the bioactive compounds in catechins isolation and its components from green tea GMB-4 ***:Green tea GMB-4 clones were extracted with distilled water at *** were eluted into the column with 10%***,the column was eluted with95%ethanol and evaporated *** tea extract was identified by thin layer *** were separated by the stationary phase in column chromatography using polyamide with 10%ethanol eluent and 95%*** results of isolations were analyzed by high performance liquid chromatographic(HPLC)and LCMS/*** of catechins by HPLC was done by external ***:Fraction from 10%ethanol showed that four major peaks at retention time of1.663,2.367,2.950 and 4.890,indicated the presence of four catechins components including catechin,epicatechins,gallocatechin and ***,fraction from 95%ethanol showed two main peaks at retention time of 5.167 and 9.82,which indicated the presence of epigallocatechin gallate(EGCG)and epicatechin gallate(ECG).EGCG(m/z 459),epigallocatechin(m/z 307),ECG(m/z 443),and epicatechin(m/z 291)were isolated and separated successfully using HPLC and LC-MS/***:The HPLC and LC-MS/MS methods were successfully tuned for the qualitative analysis of green tea extract with EGCG and *** major catechins were separated and identified by LC-MS/MS,such as EGCG,epigallocatechin,ECG and *** result of HPLC analysis showed that EGCG and ECG were main components from catechins isolation of green tea GMB-4 clone.

关键词： Catechins Green tea GMB-4 clone LC-MS/MS HPLC

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