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Assessment of periportal fibrosis in Schistosomiasis mansoni patients by proton nuclear magnetic resonance-based metabonomics models

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World Journal of Hepatology 2022年第4期14卷 719-728页

作者： milena Lima rodrigues Tatiane Priscila Santos rodrigues da Luz Caroline Louise Diniz Pereira Andrea Dória Batista Ana Lúcia Coutinho Domingues Ricardo Oliveira Silva Edmundo Pessoa Lopes Programa de Pós-Graduação em medicina Tropical Centro de Ciências MédicasUniversidade Federal de PernambucoRecife 50670-901PernambucoBrazil Programa de Pós-Graduação em Química Centro de Ciências Exatas e da NaturezaUniversidade Federal de PernambucoRecife 50670-740PernambucoBrazil Hospital das Clínicas Departamento de Medicina ClínicaUniversidade Federal de PernambucoRecife 50670-901PernambucoBrazil

BACKGROUND The evaluation of periportal fibrosis(PPF)is essential for a prognostic assessment of patients with Schistosomiasis *** WHO Niamey Protocol defines patterns of fibrosis from abdominal ultrasonography,^(1)H-nuclear magnetic resonance(NmR)-based metabonomics has been employed to assess liver fibrosis in some *** To build 1H-NmR-based metabonomics models(mm)to discriminate mild from significant periportal PPF and identify differences in the metabolite *** A prospective cross-sectional study was performed on schistosomiasis patients at a University Hospital in Northeastern *** evaluated 41 serum samples from 10 patients with mild PPF(C Niamey pattern)and 31 patients with significant PPF(D/E/F Niamey patterns).mm were built using partial least squares-discriminant analysis(PLS-DA)and orthogonal projections to latent structures discriminant analysis(OPLS-DA)*** PLS-DA and OPLS-DA resulted in discrimination between mild and significant PPF groups with R2 and Q2 values of 0.80 and 0.38 and 0.72 and 0.42 for each model,*** OPLS-DA model presented accuracy,sensitivity,and specificity values of 92.7%,90.3%,and 100%to discriminate significant *** metabolites identified as responsible by discrimination were:Nacetylglucosamines,alanine,glycolaldehyde,carbohydrates,and *** mms discriminated mild from significant PPF patterns in patients with Schistosomiasis mansoni through identification of differences in serum metabolites profiles.

关键词： metabolomics Portal hypertension Schistosoma mansoni Biomarkers Neglected disease Nuclear magnetic resonance

Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01

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Journal of Forestry Research 2020年第2期31卷 507-519页

作者： Gisela manuela de Franca Bettencourt Carlos Ricardo Soccol Thais Salete Giovanella Luziane Franciscon Daiane Rigoni Kestring Isabel rodrigues Gerhardt Juliana Degenhardt-Goldbach Department of Bioprocess Engineering and Biotechnology Federal University of ParanaAvenida Cel.Francisco Heraclito dos Santos210CuritibaParanaBrazil Department of Agronomy Federal University of ParanaRua dos Funcionarios1540CuritibaParanaBrazil Embrapa Forestry Estrada da RibeiraKm 111ColomboParanaBrazil Embrapa Agricultural Informatics Av.Andre Tosello209CampinasSao PauloBrazil

Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus;however,the efficiency is still low for most species,so many researchers still use seeds instead of clones as initial *** work aimed to develop a genetic transformation protocol,based on a highly efficient in vitro organogenesis protocol,for an Eucalyptus urophylla clone selected in our breeding *** growth regulators were evaluated for indirect organogenesis and *** a two-step protocol,the combination of callus induction media supplemented with 0.5 μm thidiazuron+0.5 μm naphthaleneacetic acid(NAA)and shoot induction media supplemented with 5.0 μm benzylaminopurine+1.0 lm NAA allowed up to 85.6%shoot formation with more shoots per explants when compared with other *** plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance.A RAPD analysis was used to check for somaclonal *** tests using 11 RAPD primers,we did not observe somaclonal variation in the in vitro stages evaluated.

关键词： Eucalyptus Genetic transformation In vitro regeneration Plant growth regulators Somaclonal variation

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古翼手龙类的德国翼手龙新标本(英文)

地球学报 2010年第S1期31卷 57-58页

作者： Taissa rodrigues Alexander W.A.KELLNER Oliver W.m.RAUHUT Setor de Paleovertebrados DGPMuseu Nacional/Universidade Federal do Rio de Janeiro.Quinta da Boa Vista s/nSao CristóvaoRio de JaneiroRJ20940-040Brazil Bayerische Staatssammlung für Palaontologie und Geologie Richard-Wagner-Strasse 1080333MunichGermany

The pterodactyloid genus Germanodactylus is rela-tively poorly known. Two species have been described, although it has been recently suggested (Wang et al., 2008)

关键词：翼手龙类德国标本

Regulation of ABCB1 activity by microRNA-200c and microRNA-203a in breast cancer cells: the quest for microRNAs’ involvement in cancer drug resistance

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Cancer Drug Resistance 2019年第3期2卷 897-911页

作者： Ana Armada Bruno Costa Gomes miguel Viveiros Jose Rueff António Sebastião rodrigues Global Health and Tropical medicine Instituto de Higiene e Medicina TropicalUniversidade NOVA de LisboaRua da Junqueira 100Lisbon 1349-008Portugal Centre for Toxicogenomics and Human Health GeneticsOncology and Human ToxicologyNOVA Medical School/Faculdade de Ciências MédicasUniversidade Nova de LisboaRua Câmara Pestana 6LisbonLisbon 1150-008Portugal.

Aim:ABCB1 is a major player in cancer drug *** purpose of this study was to functionally assess the regulation of ABCB1 activity in a doxorubicin-resistant breast cancer cell line by miR-200c and ***:Human breast carcinoma cell lines mCF-7(Doxorubicin-sensitive and not expressing ABCB1)and KCR(Doxorubicin-resistant and expressing ABCB1)were used to evaluate the expression levels of miR-200c and miR-203 by Real-time quantitative PCR(RT-qPCR).The effects of transient ectopic expression of miRNA-200c and miR-203 on the expression of ABCB1 in KCR and mCF-7 cells was verified by RT-qPCR and Western *** extrusion activity of the ABCB1 pump was analyzed by fluorescence microscopy and flow cytometry through fluorescence substrate retention assays(DiOC2)in the presence and absence of the ABCB1 inhibitor ***:RT-qPCR results indicated a 100,000-fold increase in ABCB1 mRNA expression levels in KCR cells compared to mCF-7 cells,and is inversely correlated with the expression of miR-203 and *** insertion of miR-200c and miR-203 led to a higher retention of DiOC2 within KCR cells,and slightly reduced the protein levels of ABCB1 in KCR cells,although the high initial expression of ABCB1 masked the reduction in protein *** increased intracellular accumulation of the fluorescent due DiOC2 in the presence of the ABCB1 inhibitor verapamil correlated with the inhibition caused by miR-203 and miR-200c in transfected ***: The present study demonstrates that miR-200c and miR-203 exert a negative modulating effect on the activity of ABCB1 associated with doxorubicin resistance.

关键词： ABCB1 drug resistance drug extrusion breast cancer miR2003 miR203

CYP1A1,CYP2E1 and EPHX1 polymorphisms in sporadic colorectal neoplasms

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World Journal of Gastroenterology 2016年第45期22卷 9974-9983页

作者： Glaucia maria m Fernandes Anelise Russo marcela Alcantara Proenca Nathalia Fernanda Gazola Gabriela Helena rodrigues Patrícia matos Biselli-Chicote Ana Elizabete Silva Joao Gomes Netinho érika Cristina Pavarino Eny maria Goloni-Bertollo Genetics and molecular Biology Research Unit-UPGEm Department of Molecular Biology Sao José do Rio Preto Medical School FAMERP Departament of Biology UNESP-Sao Paulo State University Departament of Surgery and Coloproctology Service Sao José do Rio Preto Medical School FAMERP

AIm To investigate the contribution of polymorphisms in the CYP1A1, CYP2E1 and EPHX1 genes on sporadic colorectal cancer(SCRC) risk. mETHODS Six hundred forty-one individuals(227 patients with SCRC and 400 controls) were enrolled in the study. The variables analyzed were age, gender, tobacco and alcohol consumption, and clinical and histopathological tumor parameters. The CYP1A1 *2A, CYP1A1 *2C CYP2E1 *5B and CYP2E1 *6 polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The EPHX1 Tyr113 His, EPHX1 His139 Arg and CYP1A1 *2C polymorphisms were detected by real-time PCR. Chisquared test and binary logistic regression were used in the statistical analysis. Haplotype analysis was conducted using the Haploview program, version *** Age over 6 2 years was a risk factor for SCRC development(OR = 7.54, 95%CI: 4.94-11.50, P male individuals were less susceptible to SCRC(OR = 0.55, 95%CI: 0.35-0.85, P morphism was associated with SCRC in the codominant(heterozygous genotype: OR = 2.66, 95%CI: 1.64-4.32, P minant(OR = 2.82, 95%CI: 1.74-4.55, P minant(OR = 2.58, 95%CI: 1.59-4.19, P models(OR = 2.84, 95%CI: 1.78-4.52, P morphism was associated with an increased SCRC risk in codominant(heterozygous genotype: OR = 2.81, 95%CI: 1.84-4.28, P mozygous polymorphic : OR = 7. 3 2, 9 5 % C I : 1.85-28.96, P minant(OR = 2.97, 95%CI: 1.97-4.50, P minant(OR = 2.64, 95%CI: 1.74-4.01, P models(OR = 2.78, 95%CI: 1.91-4.06, P med by the minor alleles of the CYP2E1*5B(C) and CYP2E1*6(A) polymorphisms was associated with SCRC(P = 0.002). However, the CYP1A1 *2A, CYP1A1 *2C, EPHX1 Tyr113 His and EPHX1 His139 Arg polymorphisms were not associated with *** In conclusion, the

关键词： Single-nucleotide polymorphisms Colorectal neoplasms Cytochrome P-450 CYP2E1 Cytochrome P-450 CYP1A1 Epoxide hydrolases 1

Four α correlations in nuclear fragmentation: a game of resonances

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Chinese Physics C 2021年第2期45卷 406-413页

作者： m.Huang A.Bonasera S.Zhang H.Zheng D.X.Wang J.C.Wang N.Song X.Tang L.Lu G.Zhang Z.Kohley m.R.D.rodrigues Y.G.ma S.J.Yennello College of mathematics and Physics Inner Mongolia University for NationalitiesTongliao 028000China Institute of Nuclear Physics Inner Mongolia University for NationalitiesTongliao 028000China Cyclotron Institute Texas A&M UniversityCollege StationTX 77843USA Laboratori Nazionali del Sud INFNvia Santa Sofia6295123 CataniaItaly School of Physics and Information Technology Shaanxi Normal UniversityXi'an 710119China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China Chemistry Department Texas A&M UniversityCollege StationTX 77843USA Key Laboratory of Nuclear Physics and Ion-beam Application(mOE) Institute of Modern PhysicsFudan UniversityShanghai 200433China Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China

Heavy ion collisions near the Fermi energy produce a‘freezout’region where fragments appear and later decay,emitting mainly neutrons,protons,alpha particles,and gamma *** decay products carry information about the decaying *** events may result in high yields of boson particles,especially alpha particles,and may carry important information about the nuclear Bose Einstein condensate(BEC).We study‘in medium’fourαcorrelations and link them to the‘fission’of 16O in two 8Be in the ground state or 12C*(Hoyle state)+α.Using novel techniques for the correlation functions,we confirm the resonance of 16O at 15.2 meV excitation energy,and the possibility of a lower resonance,close to 14.72 *** latter resonance is the result of allαparticles having 92 keV relative kinetic energies.

关键词： 4αresonance Hoyle state Bose Einstein condensate heavy ion reactions

Native Species Composition and Diversity of Valuable Woody Plants Regeneration during Fallow Period in Kamwatta, moruca, Guyana

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Open Journal of Forestry 2023年第3期13卷 278-293页

作者： Shondell rodrigues Susy Lewis Colis Primo Faculty of Agriculture and Forestry University of Guyana Georgetown Guyana Environmental Protection Agency Georgetown Guyana

An intelligent method of shifting cultivation to regenerate vegetation after a long fallow period is critical information for restoration strategies. The literature review identified that the assessment of woody plant species on abandoned farmland has never been done before in Kamwatta, moruca Region 1. In addition, more information should be available in indigenous communities and at the national level, including more documentation of the utilization of natural resources by the Warrau community. This study assessed the valuable woody plant species that grew during the fallow periods. Nine farmlands were randomly selected along a 100 m transect, each with varying fallow periods. In order to collect information on the age of farm abandonment, the researcher employed a mixed-methods approach. First, we found how long each farm had been fallow by administering a semi-structured questionnaire. Then, we conducted a flora survey to gain information on the variety and abundance of valuable plants using the age of farm abandonment as a dependent variable. The data analysis included the number of valuable woody trees with a diameter of >10 cm, seedlings and saplings with a diameter of m, the location of identified trees, and the uses of identified tree species. microsoft Excel was used to code and analyze the questionnaire, while QGIS, Shannon’s Index of Diversity, and Simple Linear Regression were used to analyze the inventory data. The results showed that the respondents listed 38 valuable woody species, and 79% occurred in the studied area. The field verification revealed that the dominant family in the area was mimosaceae. Species abundance increased with fallow up to 10 years before declining in the latter years. Plant diversity and abundance had a weak relationship with the age of abandoned farmlands for woody trees, while the seedlings and saplings showed no relationship.

关键词： Native Woody Plants Fallow Time Forest Regeneration Species Diversity Species Abundance

Polysaccharide-based nanomedicines for cancer immunotherapy:A review

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Bioactive materials 2021年第10期6卷 3358-3382页

作者： Yujun Zeng Yufan Xiang Ruilong Sheng Helena Tomás João rodrigues Zhongwei Gu Hu Zhang Qiyong Gong Kui Luo Huaxi mR Research Center(HmRRC) Department of RadiologyDepartment of NeurosurgeryFunctional and Molecular Imaging Key Laboratory of Sichuan ProvinceNational Clinical Research Center for GeriatricsWest China HospitalSichuan UniversityChengdu610041China Research Unit of Psychoradiology Chinese Academy of Medical SciencesChengdu610041China CQm-Centro de Quimica da madeira MMRGUniversidade da MadeiraCampus da Penteada9000-390FunchalMadeiraPortugal Amgen Bioprocessing Centre Keck Graduate InstituteClaremontCA91711USA

Cancer immunotherapy is an effective antitumor approach through activating immune systems to eradicate tumors by ***,direct administration of“naked”immunotherapeutic agents(such as nucleic acids,cytokines,adjuvants or antigens without delivery vehicles)often results in:(1)an unsatisfactory efficacy due to suboptimal pharmacokinetics;(2)strong toxic and side effects due to low targeting(or off-target)*** overcome these shortcomings,a series of polysaccharide-based nanoparticles have been developed to carry immunotherapeutics to enhance antitumor immune responses with reduced toxicity and side *** are a family of natural polymers that hold unique physicochemical and biological properties,as they could interact with immune system to stimulate an enhanced immune *** structures offer versatility in synthesizing multifunctional nanocomposites,which could be chemically modified to achieve high stability and bioavailability for delivering therapeutics into tumor *** review aims to highlight recent advances in polysaccharide-based nanomedicines for cancer immunotherapy and propose new perspectives on the use of polysaccharide-based immunotherapeutics.

关键词： Polysaccharides Drug delivery systems Nanomedicines Cancer immunotherapy Anticancer efficacy

Development and challenges of cells-and materials-based tooth regeneration

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Engineered Regeneration 2022年第2期3卷 163-181页

作者： Zeyu Fu Yu Zhuang Jinjie Cui Ruilong Sheng Helena Tomás João rodrigues Bin Zhao Xudong Wang Kaili Lin Department of Oral and Cranio-maxillofacial Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyResearch Unit of Oral and Maxillofacial Regenerative MedicineChinese Academy of Medical SciencesShanghai 200011China School of materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China CQm-Centro de Quimica da madeira MMRGUniversidade da MadeiraCampus da Penteada9000-390 FunchalMadeiraPortugal School of materials Science and Engineering Center for Nano Energy MaterialsNorthwestern Polytechnical UniversityXi’an710072China

Tooth defect and loss are common clinical diseases in *** the extension of life expectancy,there is an increasing demand for tooth tissue and whole tooth *** with traditional oral prosthetic treatment,tooth regeneration has unique advantages and has become one of the hotspots towards oral biomedical *** this review,we discussed the development and challenges of tooth regeneration based on cells and materials,including tooth enamel,dentin,dental pulp,cementum,dentin-pulp complex,and the whole tooth regeneration,in order to provide a comprehensive,up-to-date,illustrative overview of tooth regeneration *** mechanisms of the regeneration were also summarized and ***,this review hints the future perspective and research direction of tooth regeneration in the challenging field of regenerative dentistry.

关键词： Tooth regeneration Cells materials Regenerative dentistry