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黄唇鱼迟缓爱德华氏菌分离鉴定及药敏分析

南方水产科学 2024年第2期20卷 140-149页

作者：李敏叶毅飞李永福谢海燕李鸿超李春枝李希国李本旺陈灼均莫介化卢伟华朱燕秋张险朋东莞市动物疫病预防控制中心广东东莞523086 东莞市人兽共患病重点实验室广东东莞523086

2023年入夏以来,东莞市黄唇鱼(Bahaba taipingensis)自然保护区救护基地出现黄唇鱼发病和死亡病例,症状主要为食欲下降,体表发红、充血,眼球突出,角膜白浊溃烂,吻部溃烂;解剖后发现腹腔有大量腹水,肝肿大呈褐色。为确定其病因,针对性制... 详细信息

2023年入夏以来,东莞市黄唇鱼(Bahaba taipingensis)自然保护区救护基地出现黄唇鱼发病和死亡病例,症状主要为食欲下降,体表发红、充血,眼球突出,角膜白浊溃烂,吻部溃烂;解剖后发现腹腔有大量腹水,肝肿大呈褐色。为确定其病因,针对性制定防治措施,采集死亡病鱼的心脏、肝脏、脾脏、肾脏、肠、鳃丝等组织样品。从病死鱼体内分离到一株优势菌株,命名为DG230920,通过对菌株的形态特征、生理生化特性和16S rRNA基因序列综合分析,鉴定该菌为迟缓爱德华氏菌(Edwardsiella tarda)。病理组织学检测结果显示,心脏、肝脏、脾脏、肾脏和鳃均有明显的炎症反应,肠出血、黏膜上皮细胞脱落,脾淋巴细胞稀疏、内皮细胞变性坏死,肾小管变性坏死,鳃见鳃丝脱落、出血。对分离菌株进行药敏分析,结果为对环丙沙星、左氧氟沙星高度敏感,最低抑菌质量浓度仅≤0.25 mg·mL^(-1);其次是厄他培南,最低抑菌质量浓度≤0.5 mg·mL^(-1)。结果表明,黄唇鱼发病原因可能是迟缓爱德华氏菌感染,分离菌株遗传序列稳定,对临床使用的抗菌药物均为敏感。研究提示,应加强黄唇鱼迟缓爱德华氏菌感染的防治,选用敏感药物科学治疗。

关键词：黄唇鱼迟缓爱德华氏菌细菌分离鉴定病理组织切片药物敏感分析

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Effect of Taichong(LR 3) acupuncture in spontaneously hypertensive rats

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Journal of Traditional Chinese Medicine 2019年第1期39卷 74-80页

作者： Luo Xiaozhou Huang Jianting Yu Juan Tang chunzhi Department of Rehabilitation Chengdu Second People's HospitalChengdu 610000China Department of Rehabilitation Medicine The First Affiliated Hospital of Shenzhen University School of MedicineShenzhen Second People's HospitalFutian DistrictShenzhen 518060China College of Acupuncture and Tuina Hunan University of Traditional Chinese MedicineChangsha 410000China Clinical Medical College of Acupuncture Moxibustion and RehabilitationGuangzhou University of Traditional Chinese MedicineGuangzhou 510000China

OBJECTIVE: To evaluate the effects of Taichong(LR3) acupuncture points(acupoints) on the expression of glucose transporter protein 1(GLUT1) in the hypothalamus of spontaneously hypertensive rats(SHRs) as measured by combined positron emission tomography and computed tomography(PETCT).METHODS: Spontaneously hypertensive rats(SHR)were divided into model, Taichong(LR 3) acupuncture, and sham groups. Additionally, Tokyo Wistar rats were used as the control group. Changes in blood pressure were recorded in different groups of rats before and after the corresponding treatment. Hematoxylin and eosin(HE) staining was used to study basic morphological changes, and immunohistochemistry was used to determine GLUT1 expression in the hypothalamus. Further,PET-CT was utilized to elucidate the antihypertensive mechanism after acupuncture at the Taichong(LR 3) ***: PET-CT indicated activation of the hypothalamus. Measurement of blood pressure showed that acupuncture at the Taichong(LR 3) acupoints lowered blood pressure. HE staining did not show any significant pathological changes, although differences in cell number were observed. Immunohistochemical analysis indicated a GLUT1 downregulation in the SHRs of the Taichong(LR 3) acupuncture group after the ***: Acupuncture at Taichong(LR 3) acupoints lowered blood pressure in SHRs, with possible mechanisms being changes in cell number and GLUT1 expression in the hypothalamus.

关键词： Hypertension Acupuncture Point LR 3(Taichong) Positron emission tomography computed tomography Glucose transporter type 1 Rats,inbred SHR

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超声弹性成像诊断颈部淋巴结核的价值

中国临床医学 2011年第3期18卷 304-306页

作者：王志远吴泽惠谭永刚杨通明周纯芝梁霞彭惠胡丽娜刘军湖南省肿瘤医院超声科湖南长沙410011 湖南省胸科医院超声科湖南长沙410013 中南大学湘雅第二医院影像科湖南长沙410011

目的:研究颈部淋巴结结核的超声弹性成像表现,评价超声弹性成像在其诊断中的价值。方法:采用弹性5分评分法对44例97个颈部淋巴结结核病灶进行分析。结果:有85.7%(30/35)结节型和83.7%(36/43)干酪坏死型颈部淋巴结结核的超声弹性成像评... 详细信息

目的:研究颈部淋巴结结核的超声弹性成像表现,评价超声弹性成像在其诊断中的价值。方法:采用弹性5分评分法对44例97个颈部淋巴结结核病灶进行分析。结果:有85.7%(30/35)结节型和83.7%(36/43)干酪坏死型颈部淋巴结结核的超声弹性成像评分和为1～2分,有57.8%(11/19)愈合钙化型的弹性成像评分为3～4分。结论:绝大多数颈部淋巴结结核病灶硬度较小,超声弹性成像评分较低,有助于诊断。

关键词：超声弹性成像颈部淋巴结结核

“化浊调肝健脾”法对乙型肝炎病毒相关性肝硬化指标影响的临床研究

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中国中西医结合消化杂志 2024年第2期32卷 115-119页

作者：苏春芝杨倩张金丽孟宪鑫张云凤苏飞张桐张瑜河北省中医院肝病科石家庄050011 河北省中医院脾胃病二科河北省浊毒证重点实验室

目的:观察化浊调肝健脾法治疗乙型肝炎病毒相关性肝硬化(HBV-LC)患者的临床疗效。方法:采用随机数表法将112例HBV-LC患者分为“化浊调肝”治疗组(治疗组)和对照组,每组各56例。对照组给予恩替卡韦分散片抗病毒治疗,治疗组在对照组的基... 详细信息

目的:观察化浊调肝健脾法治疗乙型肝炎病毒相关性肝硬化(HBV-LC)患者的临床疗效。方法:采用随机数表法将112例HBV-LC患者分为“化浊调肝”治疗组(治疗组)和对照组,每组各56例。对照组给予恩替卡韦分散片抗病毒治疗,治疗组在对照组的基础上加用化浊调肝健脾汤,2组均治疗3个月。治疗结束后,比较2组患者治疗前后的临床疗效、中医症状总积分、肝功能、血清肝纤维化四项、Fibrotouch肝脏硬度测量值及外周血炎症因子水平的变化。结果:治疗3个月结束后,治疗组临床疗效总有效率高于对照组,中医症状总积分低于对照组(P<0.05)。2组患者谷草转氨酶(AST)、谷丙转氨酶(ALT)、γ-谷氨酰基转移酶(GGT)、总胆红素(TBIL)、层粘连蛋白(LN)、透明质酸(HA)、Ⅲ型前胶原氨基端肽(PCⅢNP)、Ⅳ型胶原(C-Ⅳ)、肝脏弹性指数、IL-17A、IL-6水平在治疗后均下降,且治疗组各项指标低于对照组(P<0.05)。2组患者IL-10水平在治疗后升高,且治疗组高于对照组(P<0.05)。结论:以“化浊调肝健脾”法则治疗HBV-LC可获得更好的临床疗效,有效改善患者中医症状总积分、肝功能及肝纤维化指标,降低炎症因子水平,延缓肝硬化病情进展。

关键词：乙型肝炎病毒相关性肝硬化化浊调肝健脾炎症因子

A MULTI-COUPLED FINITE ELEMENT ANALYSIS OF RESISTANCE SPOT WELDING PROCESS

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Acta Mechanica Solida Sinica 2006年第1期19卷 86-94页

作者： Hou Zhigang Wang Yuanxun Li chunzhi Chert Chuanyao School of Civil Engineering and Mechanics Huazhong University of Science and Technology Wuhan 430074 China School of Mechtronic and Automobile Engineering Yantai University Yantai 264005 China

A two-dimensional axisymmetric finite element model is developed to analyze the transient thermal and mechanical behaviors of the Resistance Spot Welding （RSW） process using commercial software ANSYS. Firstly a direct-coupled electrical-thermal Finite Element Analysis （FEA） is performed to analyze the transient thermal characteristics of the RSW process. Then based on the thermal results a sequential coupled thermo-elastic-plastic analysis is conducted to determine the mechanical features of the RSW process. The thermal history of the whole process and the temperature distribution of the weldment are obtained through the analysis. The mechanical features, including the distributions of the contact pressure at both the faying surface and the electrode-workpiece interface, the stress and strain distributions in the weldment and their changes during the RSW process, the deformation of the weldment and the electrode displacement are also calculated.

关键词： Finite Element Analysis (FEA) Resistance Spot Welding (RSW) electrical-thermal coupling thermo-elastic-plastic analysis thermal behavior mechanical feature

Enormous Promotion of Photocatalytic Activity through the Use of Near-Single Layer Covalent Organic Frameworks

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CCS Chemistry 2022年第7期4卷 2429-2439页

作者： Xiaomin Ren chunzhi Li Wanchao Kang He Li NaTa Sheng Ye Linyan Hu Xiuli Wang Can Li Qihua Yang State Key Laboratory of Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023 University of Chinese Academy of Sciences Beijing 100049 State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhouGansu 730000

Enhancing the charge separation efficiency is highly effective strategy in improving the photocatalytic activity of covalent organic frameworks(COFs)which have the problems of low conductivity and difficult dissociation of *** this work,we report the sevenfold increase in apparent quantum efficiency resulting from the use of a near-single layer COF(SLCOF)in photocatalytic hydrogen evolution compared with bulk *** by transient absorption spectroscopy characterization,100% of photogenerated long-lived electrons in the near-SLCOF can be extracted and participate in the photocatalytic ***,the electron extraction efficiency declined to only about 11% when the COFs were increased to eight layers,implying the difficulty of charge migration among COFs *** near-SLCOF was prepared by deposition of selfexfoliated COFs colloids on SiO_(2),driven by their strong *** work not only sheds light on the significant influence of COF layer thickness on the charge separation efficiency but also provides a new route to prepare and stabilize COF layers for practical applications.

关键词： near-single layer covalent organic frameworks excitons H_(2)evolution photocatalysis TAS

单环刺螠ACE抑制肽的分离、鉴定及活性评价

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中国海洋药物 2024年第3期43卷 51-58页

作者：聂鹏孟乐李针徐炫任春芝唐红军王玉梅孙坤来浙江海洋大学食品与药学学院浙江舟山316022 舟山市妇幼保健院浙江舟山316000 四川聚元药业集团有限公司四川达州635000

目的以单环刺螠(Urechis unicinctus)为原材料,以血管紧张素转化酶(ACE)抑制活性为筛选指标,运用酶解技术和现代分离纯化技术,制备具有降压潜能的ACE抑制肽。方法运用可控酶解技术,以ACE的抑制率为筛选指标,优化酶解条件。运用超滤、S... 详细信息

目的以单环刺螠(Urechis unicinctus)为原材料,以血管紧张素转化酶(ACE)抑制活性为筛选指标,运用酶解技术和现代分离纯化技术,制备具有降压潜能的ACE抑制肽。方法运用可控酶解技术,以ACE的抑制率为筛选指标,优化酶解条件。运用超滤、Sephadex G-15凝胶柱层析、反相高效液相色谱及质谱测序等一系列分离纯化和鉴定技术,制备具有ACE抑制活性的生物活性肽,并通过Lineweaver-Burk法确定其抑制剂类型。结果最佳酶解条件为:胰蛋白酶,37℃、p H 8.0、加酶量4.0%、料液比1∶30、酶解时间4.0 h。通过超滤、Sephadex G-15凝胶层析和反相高效液相色谱(RP-HPLC)进行分离纯化,得到5条活性肽,经质谱和氨基酸序列分析确定其分别为:Pro-Gln-Met-Thr-Phe(DHCY1, 622.75 Da)、Pro-Tyr-Phe-Lys-His(DHCY2,690.8 Da)、Pro-Gly-Trp-Lys-Ala(DHCY3, 557.66 Da)、Pro-Gln-Gly-Met-Ile-Val-Val(DHCY4, 742.94 Da)、Val-Met-Arg-Ile-Ile(DHCY5, 630.86 Da)。其中DHCY1、DHCY4、DHCY5表现出较好的ACE抑制活性,其抑制率分别为(86.26±0.85)%、(92.11±1.13)%、(96.51±1.04)%。经判断,DHCY5为竞争性ACE抑制剂。结论胰蛋白酶酶解单环刺螠蛋白,可以产生具有显著ACE抑制活性的酶解肽,包括ACE竞争性抑制剂,具有潜在的降血压活性,可为单环刺螠酶解肽的开发利用和降血压产品的开发提供理论依据和参考。

关键词：单环刺螠酶解肽 ACE抑制活性分离纯化

Chaos in coupled atom–molecular Bose–Einstein condensates

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Communications in Theoretical Physics 2023年第4期75卷 16-23页

作者： Haiming Deng Jianquan Huang Dajun Lei chunzhi Jiang Chao Kong School of Physics and Electronic-Electrical Engineering Xiangnan UniversityChenzhou 423000China

We investigate the chaotic dynamics of a coupled three-level atom–molecular Bose–Einstein condensate system composed by one molecular mode and two atomic *** the increase of atom–molecular coupling strength,we reveal the emergence of chaotic oscillations of the relative population difference between two atomic modes,which can be proven by the broad windows with a huge number of frequencies in spectral density and the chaotic trajectories in phase space *** different effects of initial states on atomic population oscillations are revealed,where for more particles in the initial state of the molecular model,chaos appears in the larger parameter region of system ***,we find that strong intermolecular interaction strength can suppress chaos resulting from strong atom–molecule *** is due to the difficult transformation between atomic and molecular modes,as well as the relatively independent dynamic evolution of atoms and molecules.

关键词： atom-molecular Bose-Einstein condensates dynamics chaos atom-molecular coupling spectral density

机器学习法优化髋关节置换术围手术期治疗策略

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东南大学学报（医学版） 2024年第2期43卷 229-236页

作者：费俊梁马成王黎明蒋纯志李旭祥王思娜赵杨曾逸文南京医科大学附属南京医院/南京市第一医院骨科江苏南京210006 南京医科大学公共卫生学院生物统计学系江苏南京211112

目的:基于机器学习法建立预测模型,探讨其对老年股骨颈骨折髋关节置换术围手术期输血和进入ICU的预测价值。方法:分析南京市第一医院2012年1月至2021年12月的股骨颈骨折行髋关节置换患者500例的临床资料,建立髋关节置换术后输血的预测... 详细信息

目的:基于机器学习法建立预测模型,探讨其对老年股骨颈骨折髋关节置换术围手术期输血和进入ICU的预测价值。方法:分析南京市第一医院2012年1月至2021年12月的股骨颈骨折行髋关节置换患者500例的临床资料,建立髋关节置换术后输血的预测模型和进入ICU的预测模型,并且评估不同模型的预测效能。探讨影响髋关节置换术围手术期治疗的危险因素,绘制受试者工作特征(ROC)曲线,使用ROC曲线下面积(AUC)、准确率、灵敏度、特异度和F1得分来评价模型的预测性能,获得预测性能最佳的模型预测变量的重要性评分。结果:以输血为结局变量,平衡数据前随机森林的AUC值、准确率及特异度均是4个模型中最高的;平衡数据后支持向量机的AUC值、准确率、特异度以及F1得分均是最高的。以是否进ICU为结局变量,平衡数据前随机森林算法的AUC值最高,随机森林算法表现较好;平衡数据后支持向量机算法的AUC值和F1得分最高,表现最好。以是否输血为结局变量,预测变量重要性评分结果显示,术前血红蛋白和术前肌酐具有较高的重要性。以是否进ICU为结局变量,预测变量重要性评分结果显示,平衡数据前术前血红蛋白、年龄和术前肌酐具有较高的重要性;而平衡数据后术前肌酐和术前白蛋白具有较高的重要性。结论:围手术期重点关注患者的年龄、术前血红蛋白、术前肌酐、术前白蛋白,加强髋关节置换术围手术期管理,有助于老年股骨颈骨折患者的恢复,减少并发症。

关键词：机器学习股骨颈骨折髋关节置换术输血 ICU 危险因素预测模型